Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,689,048	C→T	9.5%	V295M (GTG→ATG)	CJ019_04426 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,689,048	0	C	T	9.5%	125.7 / 3.4	42	V295M (GTG→ATG)	CJ019_04426	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (14/24);  new base T (2/2);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAGGTGCTTCGACAGCAAGT  >  KT2440/4688916‑4689167                                                                                                                                     |                                                                                                                        gTGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCg                                                                                                                       >  2:350928/1‑135 (MQ=255) gTGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCg                                                                                                                       <  2:304997/135‑1 (MQ=255) gTGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACATTCTCCAGCTTGCCCATCCCCAGATCCGCCAACGCTCCCTGCTGGATTTCAGGAAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCg                                                                                                                       >  1:290621/1‑135 (MQ=255)  tGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCgg                                                                                                                      <  7:284798/135‑1 (MQ=255)       aCGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACtg                                                                                                                 >  3:381573/1‑135 (MQ=255)             accaTCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCaaa                                                                                                           >  6:328656/1‑135 (MQ=255)              ccaTCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCATCGGGACTGTGCAAAt                                                                                                          >  2:353166/1‑135 (MQ=255)              ccaTCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCATCGGGACTGTGCAAAt                                                                                                          >  2:347927/1‑135 (MQ=255)                      cATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCCCCGGGACTGTGCAAATGCAGAAGt                                                                                                  >  6:219539/1‑135 (MQ=255)                         cGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGc                                                                                               <  7:174836/135‑1 (MQ=255)                         cGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGc                                                                                               <  3:212622/135‑1 (MQ=255)                                  gAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCtt                                                                                      <  7:222080/135‑1 (MQ=255)                                   aaTCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCttc                                                                                     >  5:182118/1‑135 (MQ=255)                                             tcCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATg                                                                           >  2:23084/1‑135 (MQ=255)                                                aGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGATGTATTTCATGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTATTCATTCACCATGAc                                                                         <  1:2082/134‑1 (MQ=255)                                                   ttGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGAAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGAcgcgc                                                                     <  3:373229/135‑1 (MQ=255)                                                      cccATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGAcgcgcgc                                                                   <  8:121239/134‑1 (MQ=255)                                                         aTCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGTTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTc                                                               <  5:1752/135‑1 (MQ=255)                                                         aTCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTc                                                               <  7:221909/135‑1 (MQ=255)                                                         aTCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTc                                                               <  4:61105/135‑1 (MQ=255)                                                              cAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCa                                                          >  3:326737/1‑135 (MQ=255)                                                                  tGCGCCAACGCGCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCg                                                      <  5:290293/135‑1 (MQ=255)                                                                    cgcCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGAt                                                    <  7:334885/135‑1 (MQ=255)                                                                     gcCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGAt                                                    <  3:157882/134‑1 (MQ=255)                                                                     gcCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGAt                                                    <  2:156373/134‑1 (MQ=255)                                                                     gcCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGAt                                                    <  1:350928/134‑1 (MQ=255)                                                                       cAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGAtgtg                                                 >  3:284737/1‑135 (MQ=255)                                                                         aCGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGAAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGgggg                                               >  7:186651/1‑135 (MQ=255)                                                                                ctgctgTATTTCAGGTACCGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACa                                          <  5:219539/133‑1 (MQ=255)                                                                                ctgctgTATTTCAGGTAACGCAGGGGTAACGCGCAACGTTTTCAGAACCCCGACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACa                                          <  2:290621/133‑1 (MQ=255)                                                                                ctgctgTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACa                                          <  2:29547/133‑1 (MQ=255)                                                                                ctgctgTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACa                                          <  3:68708/133‑1 (MQ=255)                                                                                ctgctgTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACa                                          <  7:235386/133‑1 (MQ=255)                                                                                         ttCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCa                                <  4:247386/134‑1 (MQ=255)                                                                                          tCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAgg                              >  2:11029/1‑135 (MQ=255)                                                                                           cAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCATCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAgg                              <  1:347927/134‑1 (MQ=255)                                                                                           cAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCATCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAgg                              <  1:353166/134‑1 (MQ=255)                                                                                                  cGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGatat                       <  6:155777/134‑1 (MQ=255)                                                                                                     aGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAg                   >  6:352423/1‑135 (MQ=255)                                                                                                               cgcAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAGGTGCTTCg           >  7:62906/1‑133 (MQ=255)                                                                                                                     gTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGATGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGCCCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAGGTGCTTCGACAGCAAg   >  5:178383/1‑135 (MQ=255)                                                                                                                      ttttCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAGGTGCTTCGACAGCAAGt  <  5:328656/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                        GTGAGCACGGCCACCATCGCTCATCGAACTGCAGAATCACTTTCTCCAGCTTGCCCATCCCCAGATGCGCCAACGCTCCCTGCTGTATTTCAGGTAACGCAGGGGTAAAGCGCAACGTTTTCAGAACCCCCACCGGGACTGTGCAAATGCAGAAGTCGCACACGTCTTCATTCACCATGACGCGCGCGCTCGACCAGTCGATGTGGGTGACAGGACTGTTCAGGCGGATATCCAGGTGCTTCGACAGCAAGT  >  KT2440/4688916‑4689167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑