| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,690,862 | T→G | 46.0% | L281R (CTG→CGG) | yhaJ_2 → | HTH‑type transcriptional regulator YhaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,690,862 | 0 | T | G | 46.0% | 3.0 / 2.7 | 27 | L281R (CTG→CGG) | yhaJ_2 | HTH‑type transcriptional regulator YhaJ |
| Reads supporting (aligned to +/- strand): ref base T (8/5); new base G (2/11); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG > KT2440/4690736‑4690979 | tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGGCTGGgcgcgc > 5:322589/1‑135 (MQ=255)tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGGCTGGgcgcgc > 3:27076/1‑135 (MQ=255)tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGgcgcgc > 6:100986/1‑135 (MQ=255)tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGgcgcgc > 4:235152/1‑135 (MQ=255) gCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGGCCGTTGGGGCTGGGcgcgcg > 1:236468/1‑135 (MQ=255) gCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGGTTGGCCTTGgcgccgg > 3:353448/1‑132 (MQ=255) cGCAGCCATTGGGCGAACCGCTGATGGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGCCCAACCGCC‑GGCGCTTGTGGGTAGACATCATCTGGATCAAGGCGCGCCCGTTGGGCCTGGGcgcgcg < 7:253352/133‑1 (MQ=255) cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGGCCGGTGGGGCGGGGCGCGCGgcgc > 3:359260/1‑135 (MQ=255) cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCGGGGCGCGcgccgc > 6:10150/1‑135 (MQ=255) gctgtGGCGGCGGTGCGGTTGGAAAAC‑TGGGCAACCCCCGGGCGTTTGGGGGTACAATTAATCTGGAACACGCCGCGCCCGTGGGGCGGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAg < 6:301120/131‑1 (MQ=255) gCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCGACCTGGAAGTCATGCGCCAGCGGTTCGCGAAGGCGccc > 8:96617/1‑135 (MQ=255) cgtggTGCGCTTCGAAAACATGGCCAACCGCC‑TGCGCTGGTGGGTAGACATCATCTGGAGCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGccccc < 3:106705/132‑1 (MQ=255) cgcggggcgcTCCGACAACATGGCCAACCGCC‑TGCGCTGGGGGTTCTACATCATCTGTATCAAGGCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGccccc < 7:96617/129‑1 (MQ=255) gtggggcgcTTCTAAAACATGCCCAACCGCG‑GGCGGTTGGGGTTCCACATAAGGTGGATCACGGCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGcccccc < 5:10150/130‑1 (MQ=255) gcTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCt < 8:263442/135‑1 (MQ=255) caTGCCCAACGGCC‑TGCGGTTGGGGGTTCAAATCATCTGCATCACGGCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCtt < 8:367456/135‑1 (MQ=255) ggCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGGCCGTTGGGCCAGGGCGCGCGGCGCCACCCGGAACGCCCGGGCCCGCCGGTAGACCAGGGGCCCCCCACGGGCAGCTATACCTTGcc > 6:336592/1‑134 (MQ=255) cGCC‑TGCGCTGGGGGTCCAACACCTTCTGGATCACGGCCCGCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTcc < 2:275642/135‑1 (MQ=255) ggTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCGACCGGGAAGTAATGCGCCAGCGGTTGGAGAAGGAGCCCCCCCCGGCCAGAGATGCCATGCACGCCTCCAGGCCCAGCAGGcc > 1:281831/1‑134 (MQ=255) acctcatcGGGATCACGGCCCGCCGGTTGGGCCGGGGCGCGCGACGCTACCTGGAACTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGGCCAGGCCCTGCAGGCGGTCGc < 7:20482/132‑1 (MQ=255) aCATCATCTGGAGCACGCCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc < 2:281831/135‑1 (MQ=255) atcatcTGCATCACGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc < 6:269285/133‑1 (MQ=255) gATCATGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCttt < 6:322589/135‑1 (MQ=255) aTCCAGTCGCGCCCGGGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCCCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTg < 5:299234/135‑1 (MQ=255) ccccggcgcgCCCGTGGGGCGGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTgg < 1:330995/129‑1 (MQ=255) ggcgggcCGGTGGGGCGTGGGCGCGCGACGCTTCCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg < 1:80033/130‑1 (MQ=255) ggcgcgCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg < 6:41970/133‑1 (MQ=255) | TGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCC‑TGCGCTTGTGGGTAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATGCGCCAGCTGTTCGAGAAGGAGCCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG > KT2440/4690736‑4690979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |