Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,874,567	A→C	33.4%	intergenic (+13/‑174)	CJ019_04568 → / → uacT_1	hypothetical protein/Uric acid transporter UacT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,874,567	0	A	C	33.4%	43.3 / 4.0	47	intergenic (+13/‑174)	CJ019_04568/uacT_1	hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (11/18);  new base C (13/4);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC  >  KT2440/4874436‑4874696                                                                                                                                    |                                                                                                                                  gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACGGGCTCGCCAAAGGCGCCCAGGTCAACTGCCACCCCCTCGCCacg                                                                                                                                 >  5:20479/1‑132 (MQ=255) gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACGGGATCGCCAAAGCCCGCCAGGTCAACTGCCAGCACCTCCCCCGGc                                                                                                                                >  7:246247/1‑135 (MQ=255)    ttCAGGCCCAAGGGTGTGCCAGCCCGTTCATGCCGCGGGGAAAAATCCCCCATATCACAACCTACCCGCGCAAGTGCGTGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc                                                                                                                             <  8:220504/135‑1 (MQ=255)      ctggCGCATGGGGTGGCCAGCCATATGATGCGGCTGGGCAATTTCACCCACATGACAAAGTATGCGCGCAAGATCGTCGGGGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCTCCTCGCCAGGCAAc                                                                                                                             <  4:98211/131‑1 (MQ=255)       aGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGAAAGCCCAAAGCGCCCAGCTCAACTGACACCCCCCCGCCCCGCCAcccc                                                                                                                          >  3:98211/1‑134 (MQ=255)          cgcAAGCGGTTGCCTGCCAGAGCATGCGGCTGGGAAACATCCCCCAGATCACCACGCACGCGCGCAAGGTCGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGccc                                                                                                                       <  7:201388/135‑1 (MQ=255)           gcATGCGGTGGCCTGCCATAGCATGCCGCTGGGCAACATAACCAATTTTACCACCTAAGAGCGAATGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGcccc                                                                                                                      <  4:279757/135‑1 (MQ=255)           gcAAGCGGTTGCCCGCCAGATCATGCCGCTGGGCAACATCACCCAGATCACCACCGAAGCGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGcccc                                                                                                                      <  8:53981/135‑1 (MQ=255)               gCGGTTGCCAGCCATATCATCCCGCTGGCCAACATCCCCCAGATTACCCTGGAAGCCCGCACGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCaa                                                                                                                  <  1:130312/135‑1 (MQ=255)                cGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAg                                                                                                                 >  4:170323/1‑135 (MQ=255)                 gggtcccagcctgATCATCCCGCTGGCAAACATCACCCATATCACCACCGAAGCGCGCAAGCGCGTGGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAgg                                                                                                                <  5:180017/130‑1 (MQ=255)                      ccagccagATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAACAGCGCAAGCTCGTCGGCGACTGGATAGCCAAAGCCGCCCCGGTCAACTCACAGCACCTAGCCCGGCAACCCGCCCCCCAAGGGatta                                                                                                           >  6:102281/1‑132 (MQ=255)                       ctgccagTTCTTGCCGCTGGGAAAATACACCCTGATCACCACCTAAGAGCTCAAGGTGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATc                                                                                                           <  8:276465/132‑1 (MQ=255)                       cacccatATCATCCCGCTGGCAAAAATCCCCCAGAGCACCACCTAAGCGCGCAAGCGCGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCa                                                                                                          <  4:372067/132‑1 (MQ=255)                         cccagAGCATGCCGCTGGGCAACATCCCCCAGATGACCACCGAAGAGCGCATGCGGGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc                                                                                                        <  3:339616/134‑1 (MQ=255)                                          ggCAACATCACCCATATCACCCCCGAAGAGCCCATGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg                                                                                       <  8:365657/135‑1 (MQ=255)                                           gCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCACGCCAAAGGCGCCCAGGTCAACTGCCAGCACCTCGCCCGGCAACCCGCCCCCCCACGGGATCACCTTCCCCGGCCGGCGCggg                                                                                      >  7:29380/1‑135 (MQ=255)                                           gCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCCACCCGCCCCCCACGGGACTCACCTTCCCCGGCACGAGCcgg                                                                                      >  3:141989/1‑135 (MQ=255)                                                            accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGc                                                                     >  7:326336/1‑135 (MQ=255)                                                            accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCACGCAACCCGCCCCCCCAGGGCATCACCTTCCCCTGCAGGAGCCGGCGTGCCCGCGACGAGGc                                                                     >  3:35792/1‑135 (MQ=255)                                                            accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTAGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGc                                                                     >  1:47844/1‑135 (MQ=255)                                                               accGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGCATCACCTTCCCCTGCAGGAGCGGGCGTGCCCGCGCAGCGGCCg                                                                   >  7:365770/1‑134 (MQ=255)                                                               accGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAAATGAAACCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTACCCCTGCAGCAGCGGGCTTGCCCGCGAACAGGccc                                                                   >  8:274889/1‑133 (MQ=255)                                                                 cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATa                                                                >  7:26887/1‑135 (MQ=255)                                                                    agagCGCAAGCTCGTCGGCGACTAGATCGCCAAAGGCGCCCAGGTCAACTGACCGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGCGGCCGAAAGcc                                                             >  1:45010/1‑135 (MQ=255)                                                                      agCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCCGGTCAACTGCCCGCACCTCGCCCGGCAACCCGCCCCCCCAGGGCATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGCAGCGGCCGCTAGcccc                                                           >  5:152402/1‑135 (MQ=255)                                                                                cgtcgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCCAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGCAGCGGCCGATAGCCCCAGCCGCAAGc                                                 >  8:272186/1‑135 (MQ=255)                                                                                cgtcgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGc                                                 >  7:312114/1‑135 (MQ=255)                                                                                   cgGCGACTGGATCGCCAAAGGCGCCCACGTCAACTGACAGCACCACGCCCGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGAGCGGGCATGCCCGCGAAGAGGCCGCTAGCCCCAGCCGCAAGCTcc                                              >  3:274092/1‑135 (MQ=255)                                                                                     gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCCCCTTCCCCGGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGCTAGCCCCCGCCTCAAGCGCCAt                                            >  2:18845/1‑135 (MQ=255)                                                                                     gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGCATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCAt                                            >  8:156424/1‑135 (MQ=255)                                                                                     gCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGACTCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGCCGCGGCCGCTAGCCCCAGCCTCCAGCTccca                                            >  1:122128/1‑133 (MQ=255)                                                                                        aCTGGATCGCCAATGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCACGGTAATCCCCTTCCCCTGCTGGCGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCAtgtg                                         <  2:90136/135‑1 (MQ=255)                                                                                             caccccAACGGCGCCCCGGTCACCGTCCAGCACCGCCCCTGCCACCCCCCCCCCCCCGGCAAACCCGTACCCCTGCTGGCGGGGGGTGGCCCGCCACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                    <  6:256699/131‑1 (MQ=255)                                                                                             aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                    <  2:47844/135‑1 (MQ=255)                                                                                             aTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                    <  8:326336/135‑1 (MQ=255)                                                                                             aTCCCCAATGGCGCCCGGGTCAACTCACACACCCTCGCCAGCCACCCCCCCCCCCAAGGGAATCACCTTCGCCGGCTGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                    <  6:15573/135‑1 (MQ=255)                                                                                                 ccAATGGCGCCCAGGCCAACTGACAGCACCTCGCCAGGCACCCCGCCCCCCATGTGATTCACCTTCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                <  3:179814/135‑1 (MQ=255)                                                                                                 caaacggCGCCCAGGTCAACTGCCCGCACCTCCCCCGGCAACCCGCCCCCCGTGGGAAGCCCCTTCCCGGGGCGGTGGGGGCTGGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac                                <  8:181596/130‑1 (MQ=255)                                                                                                  cAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGc                                                 >  8:68522/1‑117 (MQ=255)                                                                                                  cAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGc                                                 <  7:68522/117‑1 (MQ=255)                                                                                                         gcCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACaagcaagc                        >  2:221561/1‑135 (MQ=255)                                                                                                             aGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCg                    >  7:269413/1‑135 (MQ=255)                                                                                                              ggTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGa                   <  8:269413/135‑1 (MQ=255)                                                                                                                tCAACTGAACGCACGGCGCCAGGCAACCCGCCCCCCAAGGTAATCACATTCCCCTGCTGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                 <  2:45010/135‑1 (MQ=255)                                                                                                                     tcaCAGCACCGCGCCCGGCAACCCGCCCCCCACGGCAATCACCTTCCCCGGCCGGAGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCa            <  3:170323/133‑1 (MQ=255)                                                                                                                          gCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCa        >  7:5772/1‑134 (MQ=255)                                                                                                                               tCGCCCGGCACCCCCCCCCCCAGGGGAAGCACCTCCCCCGGCCGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc  <  4:141989/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGC  >  KT2440/4874436‑4874696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑