| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,377 | 0 | A | C | 53.3% | 4.0 / 10.7 | 15 | Q29P (CAG→CCG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (8/0); total (12/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAG > KT2440/3440245‑3440508 | cTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCGGGGTGTGGCAgg > 3:75376/1‑135 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCGGGCGGGGCGCCGGCACCCGGGGGGGGGGCGGCCCACGccca > 5:160516/1‑131 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCGGGCCGCGCCCCGGCACCCGGGGTGTGGGCGGCCCACCCCgat > 6:48158/1‑134 (MQ=255) aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGGGGCCGGCCCACGCCGATACGGCGCAGCt > 7:118306/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCACCGCCGATAAGGCGCCGCTCGCTGATCAGGc > 7:13875/1‑135 (MQ=255) cggTGCTGGCGCGCGCGCTTGGGCCAGCCGCCGGGTGGCGGGGGCGGTGGTGCGGGCCGGCTGGGGTGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGa < 7:113741/134‑1 (MQ=255) cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGATCAGGCCGCGGCCTTCg > 7:73660/1‑135 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCAACCCCCATAATGCGCCGCGCGCTGATCACGCCCATGGCTTAGGCAACCAg > 8:76584/1‑135 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGGCGCCGGGGGGGGGCCGGCCAACCCCGATAAGGCGCCGCGCGCGGATCAGGGCGCGGACGTCGGCCAGccg > 3:140076/1‑133 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCACGGGGGGGCCGGCCCACCCCAATAAGGCGCCGCTCCCGGCTCATGCCGCGGACTTCGGCGAGCac > 2:142352/1‑134 (MQ=255) gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGGCGCCTGGGTGGGGGCGGCCACCGCCGATAAGGCGCCGCTCGCTGCTCAGGCCGAGGACGTCGGCAAGCAGTTGGCGGAGAAgg > 3:107147/1‑135 (MQ=255) gcggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTAGCGGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGCGCGGGAGAAGTCTgcagcag > 6:155152/1‑135 (MQ=255) aGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGAGCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACTGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa < 6:205232/135‑1 (MQ=255) gtgGCAGGCCAACGCCTATAAGGCGCCGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAgctggct > 3:25891/1‑133 (MQ=255) ggCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTgaag < 4:75376/135‑1 (MQ=255) | CTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAG > KT2440/3440245‑3440508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |