Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC54.2% ‑3.9 / 12.6 27L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (5/6);  new base C (9/4);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGA  >  KT2440/4245016‑4245277
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ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCCTTGGCCCGGCGCCCGGCCGGTTCGGCGGCAACGGCCGGCAACTGAGCGGCGGGGCAGGCGTGGGGGTCCCACCCGGCGGCCCggggg                                                                                                                                 >  5:125493/1‑133 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCCGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCCACCCGGCGGCCCGGgcg                                                                                                                                 >  3:54481/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCggggg                                                                                                                                 >  7:181148/1‑133 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                             <  3:91266/135‑1 (MQ=255)
         aTCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCAACCCGGCGGCCCGGGCGCGCTCgggg                                                                                                                        >  3:167628/1‑133 (MQ=255)
                                  cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACCACTCGTCGAGGAAcagca                                                                                               >  3:52609/1‑135 (MQ=255)
                                      gcgaccgCCTTTGGCCAGGCGCCCGGCCTGTGCGGCGGCAACGGCCGGAAACGGCGCGGGGGCGCAGACTTCGCGCACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGgcg                                                                                           <  2:176814/129‑1 (MQ=255)
                                              cagtGGCCTGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGAGTGCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                                   <  8:93260/132‑1 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGAACAGCACGCGCTTGGCCt                                                                                   >  2:177688/1‑135 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCCATCGGCGCCCCCCAACTCGTGGCGGGACACCACGCGCGTGGCCt                                                                                   >  7:74668/1‑135 (MQ=255)
                                                    ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCCAGGAACACCACGCGCTTTGCCTGCTCGc                                                                             >  8:37852/1‑135 (MQ=255)
                                                        gcgcCCGGCCTGTGCGGCGGCACCGGCAGGCAAGTGCGCGTCGGCGCAGACTTCGCTCACCACCCGGGCGGCCTGGGCGCGAGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                         <  6:145604/135‑1 (MQ=255)
                                                                ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCCCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGcc                                                                 >  2:130203/1‑135 (MQ=255)
                                                                       ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGGCGAGGAACCGCAGGCGCGTTGCCTGCTCGCTGGCGCTCAGCCGGGGCAg                                                          >  6:97762/1‑135 (MQ=255)
                                                                             aaCGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                                    <  2:38060/135‑1 (MQ=255)
                                                                                 gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTGGGCGAGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCCGcgg                                                >  7:171771/1‑133 (MQ=255)
                                                                                   aggcaACTGCGCGGCGGCGCAGACTGCGCTCCCCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                              <  4:43195/135‑1 (MQ=255)
                                                                                          tGCTCGTCGGCGCAGCCTTCGCTCACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                       <  7:148511/135‑1 (MQ=255)
                                                                                             tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGGTAGGCGCCCCCCCACTCGTGGGGGGACAGCAGGCGCGTGGCCTGCTCGCGGCCGCGCAGCCGGTGCCGGTGGCCGCTGGCGCCgggctcg                                    >  2:61378/1‑130 (MQ=255)
                                                                                                          ctgcGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCa                       <  8:168727/132‑1 (MQ=255)
                                                                                                             cgcgcaccaccCCGGCGGCCCGGGCGCGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATg                     <  3:48020/129‑1 (MQ=255)
                                                                                                              gCTGACCAACCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGc                    <  8:215207/134‑1 (MQ=255)
                                                                                                                   ccACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt              <  6:68882/135‑1 (MQ=255)
                                                                                                                   ccAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt              <  4:51974/135‑1 (MQ=255)
                                                                                                                   cacccccGGCGGCCGGGGCGCGTGCGCCCCCCCACAACTCGGCGAGCAACAGCAGGCGCTTGGCCGGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt              <  2:105566/131‑1 (MQ=255)
                                                                                                                      aCCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCAGCTCGCTGCCGATCAGCCCTGGCCGGTTCCCGCTGGCGCCCGCATCGCGGCGGTAGCCCCTGCCGgtgtttc           >  2:118030/1‑132 (MQ=255)
                                                                                                                               ccAGGGCGCGATCGGTGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCAGTAACCCATGCTGGTGTAGCCGGCCTTGa  >  3:143719/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                    
CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGA  >  KT2440/4245016‑4245277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑