| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,334,688 | 0 | T | G | 50.0% | 9.5 / 13.6 | 26 | intergenic (+231/‑32) | folK/panB | 2‑amino‑4‑hydroxy‑6‑hydroxymethyldihydropteridine pyrophosphokinase/3‑methyl‑2‑oxobutanoatehydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (3/10); new base G (13/0); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCG > KT2440/5334566‑5334800 | gCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTACAGCGcc > 4:72819/1‑135 (MQ=255)gCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTACAGCGcc > 3:62024/1‑135 (MQ=255) tCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GGTTTTCAGCGcccgg > 8:50165/1‑132 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTTCAGCGCCTGAAc > 8:37150/1‑135 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTACAGCGCCTGaa > 3:121693/1‑134 (MQ=255) cGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTTGAGCGCCCGAGCg > 1:95510/1‑135 (MQ=255) tgtgGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAg < 4:62024/135‑1 (MQ=255) ggCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTAAAGCGCCGGAACGAggta > 7:66649/1‑133 (MQ=255) ccAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTc < 2:95124/135‑1 (MQ=255) aaaGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCt < 4:121693/135‑1 (MQ=255) aGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTAAACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCAGAACGAGGAATTtactac > 4:66509/1‑135 (MQ=255) tGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 1:159702/135‑1 (MQ=255) tACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGaa < 5:66397/135‑1 (MQ=255) ccGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGt < 8:180287/134‑1 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTTAC‑GCGCCGGTACCAGGAATTTGCTACCTGCGCGAAGTAAACCATaccaac > 5:20693/1‑132 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCGGCACGATGTAGTTGCTACCTGCCGGAAGTAACCCGTACCAccc > 5:50123/1‑135 (MQ=255) ccTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGc < 8:27955/135‑1 (MQ=255) ccGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCTGAACGAGGAATTTGCTACATGCCTGAAGTAAACCTTACCACCC‑TTAACCGCCGCAAgg > 7:193760/1‑135 (MQ=255) cGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGc < 6:156463/135‑1 (MQ=255) cATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTACAGCGCCTGGACGAGGAATTTCCGAACTGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCAAAAGGCCaac > 6:100219/1‑134 (MQ=255) tCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCCGGACGCGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑ATAACGGCCGCAAGGCCAAggg > 2:73401/1‑135 (MQ=255) gatgatGTGCCGCTCCGGAAGATGACTACACGCGTTGACTGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGCCAAGGGTGaaaaaa < 6:163573/135‑1 (MQ=255) gCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGCCAAGGGTGAAAAAATAACCATg > 6:105667/1‑135 (MQ=255) cGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCt < 7:91671/135‑1 (MQ=255) cGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGCAGTAACCCTTTCCACCC‑GTAACGGCCGCAAGGCCAACGGTGAAAAAATCACCATGCTGAcca > 7:24535/1‑134 (MQ=255) tACACGCGTTGAATGCAGTCGT‑GTTTTTCAGCGCCTGCAAGAGGAATTTACTACCAGCCTGAAGTAACCCATACCACCCATTAAC‑GCCGCAAGGCCCAGGGGGAAAAAAAAACCAAGCTGACCGGCGacgccccg > 2:199906/1‑130 (MQ=255) | GCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGT‑TTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCC‑TTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCG > KT2440/5334566‑5334800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |