| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,038,854 | 0 | A | C | 53.8% | 5.4 / 12.1 | 13 | F69V (TTC→GTC) | CJ019_05655 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCCGATCCAGCCGATCATCGGGCCGAATGCACGCGCCGACCAGGTAAACGAAGTACCGCTGTCAGGCTCCGCCGAGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCATCGGCAAGAAGCCCACGATGAACACGGCCGGCAGGTGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCGGTCAGGGTATAGACCGGGGCGATGGTGGAAATGCCCAGCACCACGCTGGCCAGCAGCCCCAAGCGGCCCTTGGCCAGGCCT > KT2440/6038721‑6038987 | ccgccgATCCAGCCGCTCATCGGGCCGAATGCCCGCGCCGCCCAGGTAAACGAAGTACCGCTGTCAGGCTCCGCCGAGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCCTCGGCAAGAAGCCCACGATGAac > 2:55776/1‑135 (MQ=255) cgccgATCCAGCCGATCATCGGGCCGAATGCACGCCCCGACCAGGTAAACGAAGTACCGCAGTCAGGCTCCGCCGAGTTCAGCTCGCGATAGCCCAGGGCCACCACCAGCATCGGCAAGAAGCCCACCATGAaca > 5:194932/1‑135 (MQ=255) ccGAATGCACGCGCCGACAAGTTAAAGGAAGTACCGGTGTCGGGCGCCGCCGAGTTCAGCCCGCTATCGCCCTGCGCCACCAGCACCTGCGGCATGATGCCCTCGTTCACCACGGCCGGCAGGTGGGCGCCCAcc < 4:150987/135‑1 (MQ=255) gAAGGCACGCGCCGACCAGGTAACCGAAGTACCGCTGGCCGGCTCCGCCGAGTTCAGCGCGCGATAGCCCAGGGCCACCAGCAGCAGCGGCAAGACCCCCCCGATCACCACGGCCGGCAGGTGGGCGCCCACC‑Tc < 8:87094/135‑1 (MQ=255) tggCACGCGCCGACCAGGAAAACGAATAACCGCTGCCAGCGCCGGCCGAGTTCACCTCGGGAAAGCCCAGGGCCACCAGCAGCTGCGGCAAGGAGCCCACGATGAACACGGCCGGCAGGTGGGCGCCCACC‑Tcac < 7:35937/133‑1 (MQ=255) ccgcgCCGACCAGGTACCCGAAGTACCGCTGTCGGGCCCCGCCGAGGTCAGCTCGCGATCCCCCAGGCCCACCAGCAGCATCGGCAAGAAGCCCACGATTACCACGGCCGGCAGGTGGGCGCCCACC‑Tcacgcac < 8:207468/134‑1 (MQ=255) gacttACCGCTGTCCGCCCCGGCCTAGTGCCGCGCGCTATCCCCCCGGGCCACCACCACCCGCGGAAACAAGCCCACGAGCACCACGGCCGGCAGGGGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCg < 8:91503/131‑1 (MQ=255) gacctACCGCTGTCCGCCCCCGCCGAGTTCCGCGCGCTAACCCCCAGGCCCCCCCCCAGCATCGCCAAGACCCCCCCGATCACCACGGCCGGCAGGTGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCg < 8:91500/131‑1 (MQ=255) cacgtcccGCGGGCAGGCGCCGCCGAGTCCACCGCGCTCTAGCCCCGCCCCCCCACCAGCAGCCGCAACAACCCCCCTAGTACCACGGCCGGCAGGTGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCg < 5:14263/129‑1 (MQ=255) aGTACCGCTGTCAGGCTCCGCCGAGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCATCGGCAAGAAGCCCACGATGAACACGGCCGGCAGGTGGGCGCCCCCCAACCCGCCCGGTCGGGCCAGGGCCGCCgg > 2:82496/1‑135 (MQ=255) cgccgAGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCATCGGCAAGAAGCCCACGATGAACACGGCCGGCAGGGGGGCGCCCCCC‑GCCCGCCCGGTAGGGGCCCGGGCGCCGGGCAGGGGATATCCCGGGGc > 1:194418/1‑135 (MQ=255) aGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCATCGGCAAGAAGCCCACGATGAACACGGCCGGCCGGTGGGCGCCCCCC‑ACCCGCCCCGGAGGGCCCCGGGCGCCGGGCAGGGGATAGACCGGGGCGAtgg > 5:49450/1‑135 (MQ=255) ttCAGCGCGCGAGAGCCCAGGGCCACCAGCAGCAGCGGCAACACCCCCACGATGAACACGGCCGGCAGGTGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCGGTCAGGGTATAGACCGGGGCGAtggtg < 6:47876/135‑1 (MQ=255) aGCCCAGGGCCCCCACCACCATCGCCACCACGCCCACCCTCACCCCGGCCGCCATGGGGGCGCCCACC‑TCACGCACGGGAGGGCCAAGGGCGCCGGTCAGGGTAAAGACCGGGGCGATGGTGGAAATGCCCAGca < 7:107763/135‑1 (MQ=255) gcagcaTCGGCAAGAAGCCCACGATGAACACGGCCGGCAGGTGGGCGCCCCCC‑TCCCCCACGGTAGGGCCAAGGGCGCCGGTCAGGGTTTAGTCCGGGGGGGTGGTGGTAATTCCCCGCCCCACGCGGGCcagca > 5:119784/1‑135 (MQ=255) aaCCCGGCCGGCCGGGGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCGGTCAGGGTATAGACCGGGGCGATGGTGGAAAGGCCCAGCACCACGCTGGCCAGCAGCCCCAAGCGGCCCTTGGCCAGGCCt < 5:101255/135‑1 (MQ=255) | CCGCCGATCCAGCCGATCATCGGGCCGAATGCACGCGCCGACCAGGTAAACGAAGTACCGCTGTCAGGCTCCGCCGAGTTCAGCTCGCGATAGCCCAGGGCCACCAGCAGCATCGGCAAGAAGCCCACGATGAACACGGCCGGCAGGTGGGCGCCCACC‑TCACGCACGGTAGGGCCAAGGGCGCCGGTCAGGGTATAGACCGGGGCGATGGTGGAAATGCCCAGCACCACGCTGGCCAGCAGCCCCAAGCGGCCCTTGGCCAGGCCT > KT2440/6038721‑6038987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |