Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT244044,2930T.98.6% 337.6 / ‑3.3 72intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (21/50);  minor base G (1/0);  total (22/50)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGC  >  KT2440/44157‑44420
                                                                                                                                        |                                                                                                                               
ttCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCt                                                                                                                                 >  3:165695/1‑137 (MQ=255)
ttCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACATTTAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGACCTGTGGGGGCCt                                                                                                                                 >  2:451815/1‑137 (MQ=255)
   gATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttt                                                                                                                              >  6:8498/1‑137 (MQ=255)
       atGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttttttt                                                                                                                          <  5:47073/136‑1 (MQ=255)
           aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcct                                                                                                                      <  3:234107/137‑5 (MQ=255)
           aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcct                                                                                                                      >  1:204574/1‑133 (MQ=255)
           aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcct                                                                                                                      >  3:103700/1‑133 (MQ=255)
                gCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAAt                                                                                                                >  4:178910/1‑137 (MQ=255)
                   aagtGGTCCAGTTATCCCTTTGCGGGTTCTGTTAGTAAATCGATCATTACTCATTCAGCTTGAGTGGCTTAAGTGCTGCTTTGTTTAAAGGTGTATATTTGGCGACCATTGGGCTGCTTTTTTTTGGCTATAATTCa                                                                                                             <  4:414262/134‑1 (MQ=255)
                   aaCTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCa                                                                                                             <  6:160056/137‑1 (MQ=255)
                   aaCTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCa                                                                                                             <  4:72788/137‑1 (MQ=255)
                     cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGTTTTTTTTGCTTaaattaaat                                                                                                            >  6:14749/1‑131 (MQ=255)
                     cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAg                                                                                                           >  6:348615/1‑137 (MQ=255)
                        ggCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAATGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAg                                                                                                        <  6:370106/137‑1 (MQ=255)
                         gCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAgg                                                                                                       <  5:420012/137‑1 (MQ=255)
                         gCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAgg                                                                                                       <  3:365537/137‑1 (MQ=255)
                          ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                                      <  8:6135/137‑1 (MQ=255)
                          ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCAATCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                                      <  2:72392/137‑1 (MQ=255)
                              ttATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTc                                                                                                  <  4:103700/137‑1 (MQ=255)
                                      tGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAc                                                                                          >  7:277265/1‑137 (MQ=255)
                                          gggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcat                                                                                      >  7:28867/1‑137 (MQ=255)
                                                tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATg                                                                                >  8:284405/1‑137 (MQ=255)
                                                   tAGTAAAACGATCATTACGCATTCACCTTGAGTTTCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  8:349721/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACTCATCGCATGCCg                                                                             <  5:348615/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  1:13501/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  5:50092/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  5:221210/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  5:11081/137‑1 (MQ=255)
                                                   tAGTAAAACGAGCATTACGCATTAACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGGGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  5:14749/137‑1 (MQ=255)
                                                      tAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTg                                                                          <  8:102859/137‑1 (MQ=255)
                                                        aaaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                        <  7:253894/137‑1 (MQ=255)
                                                         aaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAg                                                                       <  3:406160/137‑1 (MQ=255)
                                                         aaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAg                                                                       >  5:362616/1‑137 (MQ=255)
                                                          aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGt                                                                      <  1:155027/137‑1 (MQ=255)
                                                              gCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCAt                                                                  <  2:273760/137‑1 (MQ=255)
                                                              gCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCAt                                                                  <  7:284405/137‑1 (MQ=255)
                                                                aTTACGCTTTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGACTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  3:125130/137‑1 (MQ=255)
                                                                aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  5:283330/137‑1 (MQ=255)
                                                                aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  1:356165/137‑1 (MQ=255)
                                                                aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  3:189775/137‑1 (MQ=255)
                                                                  tACGCATTCACCTTGAGTGGCTTAACTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCGTAAAATTAAAGTAGGCGTTCGATTGTACGCATACCATGACGGTGAAGTtgatgaaa                                                              >  2:438582/1‑135 (MQ=255)
                                                                   aCGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTa                                                             >  8:233160/1‑137 (MQ=255)
                                                                    cGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTac                                                            <  1:378766/137‑1 (MQ=255)
                                                                     gCATTCACCTTGAGTTGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTaca                                                           <  6:421322/137‑1 (MQ=255)
                                                                       aTTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACAcc                                                         >  6:138233/1‑137 (MQ=255)
                                                                         tCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                       <  2:303457/137‑1 (MQ=255)
                                                                         tCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                       <  2:204574/137‑1 (MQ=255)
                                                                              ttGAGAGGCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGACTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                                  <  6:151844/137‑1 (MQ=255)
                                                                               tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCa                                                 <  5:205547/137‑1 (MQ=255)
                                                                                 aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt                                               <  8:135903/137‑1 (MQ=255)
                                                                                  gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTATGCGTTCGATTGTACGCATCGCATTCCGGTGAAGTTAATGACTACACCAATGACCAGta                                              >  1:386600/1‑136 (MQ=255)
                                                                                        tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                       <  4:226257/122‑1 (MQ=255)
                                                                                        tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                       >  3:226257/1‑122 (MQ=255)
                                                                                        tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCa                                        >  5:225107/1‑137 (MQ=255)
                                                                                         aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCaa                                       >  2:353992/1‑137 (MQ=255)
                                                                                          aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                >  2:25104/1‑111 (MQ=255)
                                                                                          aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  1:25104/111‑1 (MQ=255)
                                                                                          aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAt                                      >  5:4823/1‑137 (MQ=255)
                                                                                            tGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                                    <  3:178910/137‑1 (MQ=255)
                                                                                                ccTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGc                                >  7:364066/1‑137 (MQ=255)
                                                                                                 cTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                               <  4:39217/137‑1 (MQ=255)
                                                                                                 atttGGTAAAAGGTGGATATTTGTCGCACATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                               <  1:416538/136‑1 (MQ=255)
                                                                                                  tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGa                              <  4:386888/137‑1 (MQ=255)
                                                                                                  tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGa                              >  4:57076/1‑137 (MQ=255)
                                                                                                       tAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCg                         >  2:31764/1‑137 (MQ=255)
                                                                                                         aaaGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                                  <  3:351469/110‑1 (MQ=255)
                                                                                                         aaaGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                                  >  4:351469/1‑110 (MQ=255)
                                                                                                              tGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGTCCAGTCTCTTCAATGTGAGCTCCCTCGCCGTCAt                  >  5:314964/1‑137 (MQ=255)
                                                                                                              tGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCAt                  >  4:397421/1‑137 (MQ=255)
                                                                                                                gATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATcc                <  3:30792/137‑2 (MQ=255)
                                                                                                                gATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt                <  7:327154/137‑1 (MQ=255)
                                                                                                                gATATTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt                <  5:138233/137‑1 (MQ=255)
                                                                                                                 atatTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg               <  7:66386/137‑1 (MQ=255)
                                                                                                                 atatTTGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg               <  8:364066/137‑1 (MQ=255)
                                                                                                                      tGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg          <  4:360572/137‑1 (MQ=255)
                                                                                                                      tGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg          <  1:250744/137‑1 (MQ=255)
                                                                                                                      tGGCGCCCATTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg          <  8:28867/137‑1 (MQ=255)
                                                                                                                              aTTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGc  <  4:165695/137‑1 (MQ=255)
                                                                                                                              aTTGGGCGCCTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGc  <  1:43220/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                               
TTCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGC  >  KT2440/44157‑44420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: