| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 434,373 | 0 | T | G | 52.6% | 1.5 / 14.5 | 19 | G68G (GGT→GGG) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base T (6/3); new base G (1/9); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCCCGCAAGGGCCGGAAGTGGTGGTCGA‑CGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGG > KT2440/434244‑434504 | accccccCAAGGGCGGGATGGGGGGGTCCC‑CGGCCCGGGGTTGCTGGCCTTCTGCAGCAAAAACACCCGGGGCCGGCCCAACCCCCCCCGGGTGACCCCCGCCGGGCCGGCCGGCGCCGCGCGgggggggggggggg < 6:398888/134‑1 (MQ=255) cccccGCAAGGGCCGGAAGGGGGGGGCGA‑CGGCCAGCGGTTGCGGGCCTTCTGCCGCAATGACAACCGGGGCCGGGCCAACCACCCCGCGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTgggggg < 4:237713/135‑1 (MQ=255) gCAAGGGCCGGAAGTGGTGGTCGAGCGGCC‑GCGGTTTGTGGGCGTCTGGCGCAAATCCGACCTGGGCCGGGGCCACCCCCCCCACGGTCTCGCCGCCCGGCCCGCCGGCGCCCCGCGCGgggggggggggggggggg > 6:145094/1‑137 (MQ=255) gCAAGGGCCGGAAGTGGTGGTCGA‑CGGCCCCCGGTTTCTGGCCGTCTGGACGAATTAAAACCAGGGCCTGGGCCACCACCCCGCGGTGATAGCCCCCTGGCAGGCCCGCGCCGCGCGCGGGGGTGTggggggggggg > 8:289706/1‑137 (MQ=255) gAAGTGGTGGTCGA‑CGGCCCCCGGTTTGTGGGCGTGTTGAGGAATGACAACCAGGGGCCGGCCAACCACCCCCAGGTGATAGCCCCCGGGCCCGCCGGCGCCGCGCGCTGGGGGGGGGGGGGGGGGGGCGCGccccc > 8:33780/1‑136 (MQ=255) cgccGGCCCGGGGGTGCGGGCCTTCTGCCCCAATGACACCCGGGGCCGGGCCAACCCCCCCGCGGGGCCCGCCGCCGGGCCGGCCGGCGCCGCGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGcc < 2:91746/134‑1 (MQ=255) ctgCGGTGGGTGGCCTTATGCAGCAATGACTACCTGGGCCTGCCCAACCACCCCGTGGGGATCGCCGCCTGGCAGGCCGGCGCCGTGCGGTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCAcc < 4:204468/135‑1 (MQ=255) gtgggggCGTTCTGCCCCAATGCCTACCTGGGCCGGGCCAACCCCCCCGGGGGGAGCGCCGCCGGGCCGGCCGGCCCCGCGCGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCa < 2:175921/132‑1 (MQ=255) tggtGGCCTTCGGCCCCAATGACAACCGGGGCCTGGCCAACCCCCCCGCGGGGAGCGCCGCCGGGCCGGCCGGCGCCGCGCGGTGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 8:283042/134‑1 (MQ=255) gggtGGCCTGTTGCCGCAAGGACTCCCGGGGCCGGGCCAACCCCCCCGTGGGGAGCCCCCGCGGGCCGGCCGGCGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 2:210959/134‑1 (MQ=255) ggCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCCCCagc > 8:322018/1‑135 (MQ=255) gCCTTCTGCCCCAATTACTCCCGGGGCCGGCCCAACCCCCCCGCGGGGTTGGCCGCCTGGCTGGCCGGGGCCGCGGGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCa < 7:33780/137‑1 (MQ=255) caATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTTGTGGGCCACAGCACCCCGCACCTGCAGGTTCAAGAgg > 5:408282/1‑137 (MQ=255) cccggggccgggccAACCCCCCCGCGGGGCTCCCCGCCGGGCCGGCCGGGGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCt < 7:289706/133‑1 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCCCAGCCCCCCGCCCCAGCAGGTTGAAGCGGCCCTGGCTGACCTCACCGgccg > 8:243713/1‑137 (MQ=255) ccccccGCGGTGCGCGCCCCCGGGCAGGCCGGCGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgccc < 5:166374/135‑1 (MQ=255) ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTGGCACCGGGTAGTTGGCCACAGCACCCCGCCCCTTCAGGTTGAAGAGTCCCTGGCTGAACTCACCGGcaccccgc > 6:242354/1‑137 (MQ=255) ccGCGGTGATCCCCGCCGGGCAGGCCGGCGCCGTGGGGTGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCcgcgc < 5:403688/137‑1 (MQ=255) aTCGCCGCCGGGCCGGCGGGGGCCGCGCGGTGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt < 4:167993/137‑1 (MQ=255) ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCCCCCTCAGGTGGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgttg > 5:60000/1‑136 (MQ=255) cAGGCCGGGGCCGAGCGCGGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGc < 1:18985/137‑1 (MQ=255) ggggggggggggggggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAt < 1:246488/131‑1 (MQ=255) gggggggggggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTggg < 7:295043/136‑1 (MQ=255) | AAGCCCGCAAGGGCCGGAAGTGGTGGTCGA‑CGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGG > KT2440/434244‑434504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |