Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 452,940 | 0 | G | C | 27.6% | 91.0 / 11.0 | 61 | intergenic (‑36/+274) | pqqB/CJ019_00410 | Coenzyme PQQ synthesis protein B/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base G (23/19); new base C (0/16); total (23/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.71e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGA > KT2440/452804‑453075 | gCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAaaaacg > 4:151955/1‑137 (MQ=255) tCAGGGTGCCATCACGGTAGCCTTTGCTGTTGACGCTGTTGCAGTTCCACTGCGGTAACCCGCCACCGGGGGCGGAGCCGATAACCTGTATTTACATGGCCACTCTCTATGCAGAAACCGGATCTAAaaaacgaaaa < 6:463890/137‑1 (MQ=255) aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCa > 7:52596/1‑137 (MQ=255) ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGt > 7:1876/1‑137 (MQ=255) ttGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATTGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGa > 3:245603/1‑137 (MQ=255) aGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAcc > 1:350917/1‑137 (MQ=255) gTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAccc > 1:411920/1‑137 (MQ=255) gTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAccc > 4:137991/1‑137 (MQ=255) gccGCAGTTGATGTACCACTGCGGGAACCCGCCACCGGGGGCGGACCCGATACCCTGGATTAACATGGCCACTCTCTATCCACAAACCGTATCCAAAAACCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTaa < 6:145548/135‑1 (MQ=255) gACGCTGTTGCAGTGCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACTAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTaa < 8:251132/137‑1 (MQ=255) gcagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGcc > 4:187968/1‑137 (MQ=255) gcagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGcc > 2:42342/1‑137 (MQ=255) tgtggcagtcCCACTGCGGGAACCCGCCACCGGGGGCGTAGCCGAGAACCTGCATGTACTTGGCCACGCTCTATCCAGAAACCGGATCGAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTg < 6:316060/133‑1 (MQ=255) tggcagttCCACGGCGGGAACCCGCCACCGGCGGCGGTGCCGAGACCCGGCATGAACATGGCCACGCTATATGCACAACCCGGAGCGAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGc < 8:24572/135‑1 (MQ=255) gcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCtt > 1:219271/1‑137 (MQ=255) cACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGtt < 8:304061/137‑1 (MQ=255) ccTGCGGGAACCCGCCACCGGCGGCGTAGCCTATAACCTGTATGTAAATGGCCACTCTATATGCACAAACCGGATCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 1:66882/136‑1 (MQ=255) aCTGCGGTAACCCGCCACCGGCGGCGTAGCCGACAACCTGGATTTACATGGCCACTCTCTATGCAGAAACCGGATCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 2:94061/137‑1 (MQ=255) aCTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCATAAACCGGATCGAAAAACCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 7:371619/137‑1 (MQ=255) ggtaaCCCGCCACGGGCGGCGTACCCCAGAACCTGTATGAACATGCCAACTCTCATTCCAGAACCCGGACCTAAAAAACTAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGaa < 7:420170/134‑1 (MQ=255) ggtaaCCAGCCACCGGGGGCGGAGCGGATAACCTTCATGTAACTGGCCACTAACAATGCAGAACCCGCATAGAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGaa < 7:243667/134‑1 (MQ=255) gAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGt > 8:258353/1‑137 (MQ=255) ccGCCACCGGCGGCGGAGCCTACTACCTGTTTGTACTTGCCCACTAACTATGCACAAACGGTATCTAAAAAACGAACACGCGCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAt < 4:433977/137‑1 (MQ=255) ccGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGAc > 1:104714/1‑137 (MQ=255) gtggggggAGCCGATAACCTGGATGTACTTGGCCACTCTCTATGCATAAACCGGATCTAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACtt < 3:360955/131‑1 (MQ=255) gAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGcc > 5:32401/1‑137 (MQ=255) ccGATAACCTGTATGTACATGGCCACTCTCTATGAAGAAACCGTATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCAAt < 6:164621/137‑1 (MQ=255) ccGACAACCTGTTTGTACATGGCCACTCACTATGCATAACCCGGATCTAAAAAACCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt < 4:438420/137‑1 (MQ=255) ccGACAACCTGTATTAAAATGCCCACTCTATATGCACAACCCGCATCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt < 1:115203/137‑1 (MQ=255) gaACCTGTATGTACATGGCCACTCTCTATGCCGAAACCGGATCTAAAAAACGAAAACGCCCCGGGAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGc < 8:443766/137‑1 (MQ=255) aaCGTGTATGAACCTGGCCACTATCTATCCACAACCCGGATCCAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 5:47568/137‑1 (MQ=255) aaCCTGGATTTACATGGCCCAGCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 6:432376/137‑1 (MQ=255) aaCCTGGATTTACATGGCCACTCTCTATCCACAACCCGGATCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 3:197650/137‑1 (MQ=255) aaCCTGGATTTACATGCCCACTCTCTATGCAGAAACCGGATCCAAAAAACGAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 2:385120/137‑1 (MQ=255) aaCCTGGATGTACATGGCCACTCTCTATGCAGAAACCGTATCTAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 5:11915/137‑1 (MQ=255) aaCCTGGATGTACATGGCCACTCTCTATGCAGAAACCGTAGCAAAAAAACTAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCa < 7:38696/137‑1 (MQ=255) aCCTGGATTTACTTGGCAACTCTAAATCCACAAACCGGATCAAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAg < 3:151955/137‑1 (MQ=255) aCCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAg < 2:350917/137‑1 (MQ=255) ccTGGATGTACATGGCCACTCTCTATCCAGAAACCGGATCGAAAAAACGAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAgg < 5:406832/137‑1 (MQ=255) cTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGt < 8:1876/137‑1 (MQ=255) tgtaTGTACATGGCCACTCTATATGCACAAACCGGACCTAAAAACCCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTc < 5:12779/134‑1 (MQ=255) tGGATTTACATGGCAACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTc < 5:206102/137‑1 (MQ=255) tGGATGTACATGGCCACTCTCTATGCAGAAACCGGAGCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTc < 5:77430/137‑1 (MQ=255) ttACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgtgt < 2:34521/136‑1 (MQ=255) aacttGGCCAGTATATATGCACAACCCGGATCGAAAAACCCAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTa < 7:195624/133‑1 (MQ=255) aCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGttt > 6:404354/1‑137 (MQ=255) aCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCCGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTCTATGCAGGttt > 6:430634/1‑137 (MQ=255) aCTCTCTATGCAGAAACCGCATCGAAAAAACGAAAACGCCCCGGCAGGCAGAGCCGTGAAAACCTAAGCCTGGCATAGAGGTTAACGAAGTACATGCTGACTTCGAAGCCGATACGCAGTTCAGTTTATGCacttta > 5:282914/1‑132 (MQ=255) ctctctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTg < 8:52596/137‑1 (MQ=255) ctctctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGCCGTGAAACCCTAAGCCTGGCTTACCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTg > 2:203421/1‑137 (MQ=255) atctctATGCACAAACCGTATCTAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTg < 3:439592/136‑1 (MQ=255) tctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCTGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTc > 8:4333/1‑137 (MQ=255) tctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTc > 5:405182/1‑137 (MQ=255) ttATTCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc < 3:140395/136‑1 (MQ=255) ctATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc < 7:258353/137‑1 (MQ=255) atATGCAGAAACCGGAGCTAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc < 6:389526/136‑1 (MQ=255) atATGCACAACCCGGATCCAAAAAACGAACACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc < 3:321476/136‑1 (MQ=255) atATGCAAAAACCGGTTTAAAAAAACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTcc < 4:245603/136‑1 (MQ=255) gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACAt > 2:443884/1‑137 (MQ=255) aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATgg > 6:201342/1‑137 (MQ=255) aCCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAAGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAAtt > 8:230190/1‑137 (MQ=255) ccGGATCGAAAAAACGAAAAAGCCCCGGCAGGCCGAGGAGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAACCTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGACCACATGGAATTa > 5:216786/1‑137 (MQ=255) tCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCt > 4:32309/1‑137 (MQ=255) cgaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGa < 7:109444/137‑1 (MQ=255) | GCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGA > KT2440/452804‑453075 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |