Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,524,7320TG44.4% 21.2 / 11.4 37Q67P (CAG→CCG) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/13);  new base G (0/16);  total (8/29)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTT  >  KT2440/1524596‑1524858
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ggACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCCGCCAGGTTCAGAAGCAGGCGCCGCGGTCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                                                >  5:24042/1‑137 (MQ=255)
         aTGGCAGTGCTCGCGGGCAGCGGTGTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCggggg                                                                                                                       <  6:412313/137‑1 (MQ=255)
              tgTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGACGGCGCTGAAAGTGCTCCTGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGTGGCGGGGGGGGGGAGGGt                                                                                                                  <  2:317784/136‑1 (MQ=255)
              tgTGCGCGCGGGCAGCTGGGGGCAACTGGCTTACCAGCAGGCGCTGCATGTGATCCTGGGGCTCGGCGTGGTGCAGCAGCAGGCGCAGCGGGCGCACCTGGCTCAGCAGGGGGGAAGGGGGCTGGGGGGGGGAGGGt                                                                                                                  <  4:250393/136‑1 (MQ=255)
                  ggcgggggCAGCCGGTTCAAAGGGGCTTTCCAGCAGGCGCTGCATGTGCTCCCGGGGCTCGGCCTGTTTCCCCAGCTGGGGCAGGGGGCGAACGTGGCGAAGCAGGGGGGATGGGGGGGGGGGGGGGGAGGGTTTGt                                                                                                              <  6:415514/132‑1 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                                             >  5:430385/1‑137 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                                             >  5:344329/1‑137 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                                             >  7:219749/1‑137 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCAGGGCGGGGGAGGGTTTGTa                                                                                                             >  6:323740/1‑137 (MQ=255)
                             ccGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATgggg                                                                                                   >  1:134921/1‑137 (MQ=255)
                             ccGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATgggg                                                                                                   >  1:129143/1‑137 (MQ=255)
                                tttGCATGTGGGTTTCCGGCTGGCGGTGAATGTGCTCCGGGGGCGGGGCGGGGTTCAGAAGCCGCGGCGGGGGGGCCCCGTGGCGCAGGGGGGGGGATGGGGGTGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTa                                                                                                <  4:420182/136‑1 (MQ=255)
                                     acgtGGCTTTCCCGCTGGCGCGGCAAGTGCGCCCGGCGCGCGGCCTGGTTCAGCAGCAGGCGCAGGGGGGGCACCTGGCGCAGCTGGGCGTATGGGGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGt                                                                                           <  4:352811/134‑1 (MQ=255)
                                               cctgcagGCGCTGCAAGGGGTCCCGGCGCTCGGCCTGGTTCCGCAGCAGGCGCAGCGGGCGCAGCTGGCGCAGCTGGGGGTAAGGTGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                 <  3:226720/134‑1 (MQ=255)
                                               ccAGCTGGCGCTTCACGGGCTCCCGGGGTTCGGCCGGTTTCAGCTGCTGGCGCTGCGTGGGCCCCTGGCGCAGCAGGGGGTAAGGTGGCTGGGGGGGGTTGGGCTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                 <  6:109720/137‑1 (MQ=255)
                                                ccgcagGCGCTGCAAGTGCTCCAGGCGCGCGGCCTGGTTCAGCCCCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTg                                                                                <  2:222898/135‑1 (MQ=255)
                                                ccgcagGCGCTGCAAGGGCGCCAGGCGCGCGGCCGGGTGCCGCCGCGGGCGCTGGGGGCGCACCGGGCGCAGCTGGGGGTAAGGGGGGGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTg                                                                                <  7:332455/135‑1 (MQ=255)
                                                 cgctggCGCTGCAAGTGCTCCCGGCGCGCGGCCTGGTTCCGCAGCAGGGGCAGCGGGCGCACCTGGCGCAGCTGGGCGTAAGGGGGCTGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                               <  4:98337/133‑1 (MQ=255)
                                                 cgcagGCGCTGCATTGGCTCCGGGCGCGCGGCCTGGTTCAGCCGCCGGCGCAGGGGGCGCCCCTGGCGCTGCAGGGCGTATGGGGGGGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                               <  4:311398/136‑1 (MQ=255)
                                                 cgcagGCGCTGCAATGGCGCCAGGCGCGCGGCCTGGGTCAGCAGCAGGCGCAGCGGGCGCACCGGGCGCAGCAGGGCGTAAGGTGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                               <  8:322049/136‑1 (MQ=255)
                                                   cagGCGCTGCAAGTGCTCCAGGCGCGCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCTCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGc                                                                             <  3:31716/137‑1 (MQ=255)
                                                     ggggcTGAAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCCGCAGGCGCCGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGcac                                                                           <  5:456444/134‑1 (MQ=255)
                                                         cTGCAAGTCCTCCAGCCGCGCGGCCTGGGTCAGCAGCAGGCGCAGCGGGCGCACCGGGCGCAGCCGGCCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTc                                                                       <  5:432675/137‑1 (MQ=255)
                                                            catgTGCTCCCGGCGCGCGGCCGGGTCCCGCCGCCGGGGCTGGGGGCCCACGGGGCGCCGCCGGGCGGATGGGGGGGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTaa                                                                    <  4:310628/134‑1 (MQ=255)
                                                             atgTGCTCCAGGCGCGCGGCCGGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGAAGCCGGGGGTAAGGGGGCTGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAAc                                                                   <  8:282937/135‑1 (MQ=255)
                                                                     ccggCGGTGGGCCTGGTGCTGCCGCAGGCGGAGGGGGCGCACGTGGCGCAGCTGGGGGTAGGGGGGCGGGGCGGGGGCGGTTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                           <  5:323740/135‑1 (MQ=255)
                                                                     ccggCGCGCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCGGGCGCAGCCGGGCGTAAGGGGGCTGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                           <  3:281731/135‑1 (MQ=255)
                                                                     ccgcggcTCTGCCTGGTGCAGCCGCAGGCGCTGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                           <  5:277319/132‑1 (MQ=255)
                                                                           cTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCtg                                                     >  8:81294/1‑137 (MQ=255)
                                                                             cGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCtgtg                                                   >  5:251734/1‑137 (MQ=255)
                                                                                                    cgggggCGCACCGGGCGCTGCAGGGGGGAAGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAccc                            <  2:241646/134‑1 (MQ=255)
                                                                                                          ggcACCTGGGGCAGCAGGGCTGAAGGTGGCGGGGGGGGGTAGGGTTTGTAGGCGATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                      <  3:257319/136‑1 (MQ=38)
                                                                                                          ggacccTGGCGCTGCAGGGCGTATGGGGGCGGTGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                      <  7:128539/133‑1 (MQ=38)
                                                                                                          cgcACGTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                      <  6:74057/137‑1 (MQ=255)
                                                                                                             acgtGGCGCAGCTGGGCGTATGGTGGGTGGGCGGGGGAGGGTTTGTAGGCCTTGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCGGGCCTCTTCGCGGGGGAACCCGCTCCTGcc                   <  8:347777/134‑1 (MQ=38)
                                                                                                                  gcgcTGCTGGGGGGAGGGTGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGt              <  6:344329/137‑1 (MQ=38)
                                                                                                                    ccagccgGGCGGAAGTTGGCGGGGCGGGGGGGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTa            <  4:231250/136‑1 (MQ=39)
                                                                                                                              ggAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCtt  <  6:30263/137‑1 (MQ=255)
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GGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTT  >  KT2440/1524596‑1524858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑