| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,740,407 | 0 | T | G | 52.2% | 0.2 / 11.0 | 23 | E53A (GAG→GCG) | CJ019_01643 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base G (0/12); total (0/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGA > KT2440/1740292‑1740530 | cTTCTCTTGGAAGGCCGGGGGCATTGTTGGCTGCATGGATGATCCTCGGGGGGGTCAGGCGTGGTATGCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCgcggc < 7:262464/137‑1 (MQ=255) ttCTCTTGGAAGGCGGGGTGCATTGTTGTCTGCATGGATGATCGTCGGGTGGGTCAGGCGTGGTTTTCGAAGGCCTCGGCCTTACGAACTATTCGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCgcggcg < 7:347616/137‑1 (MQ=21) tGCCTGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGACGGCCTCGGCCTTACTAACGATTCGAACTTGGGCGGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg < 5:60009/137‑1 (MQ=17) tGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg < 3:362007/137‑1 (MQ=255) ggATGCGCCTGGGGTGGGGCCGGCGTGGTATTCGACGGCCTGGGCCTTACGAACGATTCCAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg < 1:247403/137‑1 (MQ=17) tatCCTCGGGGGTGTCAGGGGTGTTATTCGAAGGCCTCGGCCTTACGAAGGATTCGAAGTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCcac < 4:259664/136‑1 (MQ=17) ggggtgggtCTGGCGGGGATTTCCAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCGCGGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAagaca < 4:432786/136‑1 (MQ=18) ggggtggggCCGGCGTGGTTTGCGAAGGCATCGGCCTTACGAACTTTTCGATCTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAagaca < 4:149471/136‑1 (MQ=18) gggtCCGGCGTGGTATTCGAAGGCCTCGGCCTAACGAACGATTCGAAGTTGGGCGGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa < 1:395514/137‑1 (MQ=18) gggtCAGGCGTGGTATTCGCAGGCCTCGGCCTTACGAACGATTCGAACTTGGGGGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa < 8:222584/137‑1 (MQ=255) gggtCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa < 6:437840/137‑1 (MQ=255) gggtCAGGCGTGGTATTCGAAGGCCGCGGCCTTACGAACGATTCGCACTGGGGCGGGGCGCGGCGCGGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa < 1:300650/137‑1 (MQ=18) ttttgcGATGGCCTGGGCCTTAGGAACTATTGGAACTTGGGCGGTGCGCCGCGCGGGCGCGCGGCGGTCGCGGGGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCaga < 5:223960/132‑1 (MQ=25) ttCGACGGCCTCGGCCTTACGAACTATTCGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCg < 4:55815/137‑1 (MQ=255) tgcGAAGGCCTCGGCCTTACGAACGATTGGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCg < 4:49305/135‑1 (MQ=255) gacggCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAg < 3:195393/134‑1 (MQ=255) cggCCTCGGCCTTACTAACGATTCGAACTTGGGCGGGGCGCCGCTCCGGGGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg < 2:430416/136‑1 (MQ=255) aGGCCTCGGCCTTACGAACTATTCGAACTTGGGCGTTGCGCGGCTCCGTCGCGCGGCGTTCGCGTCTCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg < 2:289726/137‑1 (MQ=255) ccTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGGGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtgatg < 5:456521/137‑1 (MQ=255) cTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtgatga < 5:208251/137‑1 (MQ=255) tCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTgatgat < 2:269709/137‑1 (MQ=255) ggCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGAtct < 8:98152/137‑1 (MQ=255) ggCGGGGCGCCGCGCCGGGGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACga < 8:423606/137‑1 (MQ=255) | CTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGA > KT2440/1740292‑1740530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |