Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,094,8080AC43.4% 12.7 / 14.1 53L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/9);  new base C (18/5);  total (39/14)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAA  >  KT2440/2094675‑2094935
                                                                                                                                     |                                                                                                                               
ttctGCGGGGGCCTTGCGGTCATGTACGGGTAGATGGGCCGGGGCAAACATTTTATCAATTTCGGTCTGCTGCTCTTCATACACCTGGGGCGCCGGCTGCTTGCCAAAATACGCCAGGCCGCGGTCGACGGCCAGgc                                                                                                                              <  2:35555/137‑1 (MQ=255)
     ccGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGcc                                                                                                                         >  4:127228/1‑137 (MQ=255)
            cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATAATGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCgg                                                                                                                  >  4:348645/1‑137 (MQ=255)
            cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATAAACCTTCGGCTCCGGCTGCTTGCGGAAATACTGCACGCCGAGCTCGCAGGGCCGGCGCGCCTGGGCgg                                                                                                                  >  1:222819/1‑137 (MQ=255)
            cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCGGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCgg                                                                                                                  <  2:13012/137‑1 (MQ=255)
                cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTg                                                                                                              >  2:17036/1‑137 (MQ=255)
                  gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCcgggcgggg                                                                                                            >  6:98850/1‑136 (MQ=255)
                      aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCgtt                                                                                                        >  3:192706/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCCGGCGCGCCTGGCCGGGCTGGGCGTTTTGCACGGcc                                                                                              >  2:304843/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGACGGCCCCGCGCGCCAGGCCGGGCTGGCCGTTTTTCAGGGcc                                                                                              >  6:13265/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGGCCGGCCGGGCGGGCGTGTTTGGAGGGcc                                                                                              >  7:294527/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGGCGTTTTTGAGGGcc                                                                                              >  7:14449/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCGGGCTGGGCGTTTTTGAGGGcc                                                                                              >  5:305983/1‑137 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCCCCGGGCCGGGCGGGGCGTTTTTCAGGGcg                                                                                              >  5:252745/1‑136 (MQ=255)
                                cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCGGTTTTTGAGGGcc                                                                                              >  5:81504/1‑137 (MQ=255)
                                     gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGGTGGGCGTTTTGGGGGGcggggtg                                                                                         >  7:17426/1‑131 (MQ=255)
                                     gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTAGGCTCCGGCTGCTTGCGCAAATACTTCAAGCCGCGCTCGAAGGCCCACCCCGCCGGGCCCGGCGGGCGCTTTTGAAGGGccgggtg                                                                                         >  4:166999/1‑132 (MQ=255)
                                        cGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCGTTTTGGAGGGCCGGTTgggc                                                                                      >  5:173109/1‑135 (MQ=255)
                                        cGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCGGTTgggc                                                                                      >  7:386504/1‑135 (MQ=255)
                                             cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCAAGCCGCGCTCGAAGGCCCGGCGCGCCTGCCCGGGCTGGCCTTTTTGCATGGCCTGTTGGCCGAGgt                                                                                 >  6:186051/1‑137 (MQ=255)
                                               ataGTTTATCAATATACGTCAGATGCTCTTCATACACCTTCGGCTCCGCCTGCTTGCTAAAATACTGCAGGCCGCGCTCTAAGGCCAGGCGCGCCTGGCGGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGgttg                                                                               <  4:206690/135‑1 (MQ=255)
                                                    ttCTCAACATCCGCCAGCTCCACAACATACACCTTCGGCACCGCCTGCTGCCCCAAATACAGCCCCCCGCGCTCGAAGGCCAGGCGCCCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaa                                                                          <  2:78715/137‑1 (MQ=255)
                                                      ctcAAGATCCGGCCGCTGCTCTACATACACCTTCGGCTCCGGCTGCTTGCGAAAATACTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                        <  6:305983/137‑1 (MQ=255)
                                                       tcAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGGCGGGCTGGCCGTTTTGGAGGGGCGGTTGGCCGGGGGTGTTgaagaa                                                                       >  3:13481/1‑137 (MQ=255)
                                                        cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCGGGCTGGCCGTTTTTCAGGGGCGGTTGGGCGAGGGTGTTgaagaaa                                                                      >  4:192947/1‑136 (MQ=255)
                                                        cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCAGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGGCCGGGCTGGCCGTTTTGAAGGGCCTGTTGGCCGAGGTTGTTGAAGAAc                                                                      >  7:251741/1‑137 (MQ=255)
                                                            aTACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGGCGGGCTGGCCTTTTTTCAGGGCCTGTTGGGCGGGGTTGTTTAATAActctt                                                                  >  4:178226/1‑136 (MQ=255)
                                                                gTCCGCTGCTCTTCACACACCTGCGGCGCCGGCTGCTTGCGCAAATAGTCCCCGCCGCGCGCGACGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                              <  7:268712/137‑1 (MQ=255)
                                                                gTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCGatatt                                                              >  4:20492/1‑136 (MQ=255)
                                                                 ccAGCTCCTATTCAAACACCTTCGGCCCCGGCTGCTGGCCAAAATCCTCCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                             <  2:16276/136‑1 (MQ=255)
                                                                  cAGCTGATCTTCAACCCCCTTCGCCTCGGGCTGCTGGCGCAAATCCTCCCCGCGGCGCGCGAAGGCCAGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgca                                                            <  3:340037/137‑1 (MQ=255)
                                                                   ggctgcgATTCATAAACCTGCGGCGCGGGATGATTGGGAAAATCATGCACGCCGCGCTCGACGGCCAGGGGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcag                                                           <  8:251741/136‑1 (MQ=255)
                                                                   cgctgctCTTCATACCCCTTCGGCTGCGGCTGTGTGCGCAAATACTGCACGCCGCGCTCGCCGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcag                                                           <  7:262227/136‑1 (MQ=255)
                                                                   aGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGGCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcag                                                           >  8:344978/1‑137 (MQ=255)
                                                                   aGCTGCTCTTCATACACCTGCGGCGCCGGCGGCTCTCGAAAATACCGCCCGCCGCGCTCGAAGGCCCGGCGCGCCTGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcag                                                           <  8:294527/137‑1 (MQ=255)
                                                                        ctctTGATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCCCGCCAGGCCGGGCTGGCCTTTTTTCAGGGGCTGTTGGGCGAGGGTGTTGAAGAACTCAATATgcatcagca                                                      >  5:27096/1‑137 (MQ=255)
                                                                                     ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGGCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                         >  5:234167/1‑137 (MQ=255)
                                                                                     ttCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCCGGCTGGGCTTTTTGCAGGGCCGGGTGGGCCAGGTTGTTTAAAAACTATATATGGAGCAGCACCAGGTTATTGCg                                         >  5:268226/1‑137 (MQ=255)
                                                                                       cGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGAGCTCGAAGGCCCGGCGCGCCGGGCCGGGGGGGGCGTTTTGCAGGGCCGGTTGGGCGCGGGTGTTGGAGAACTCTATATGAAGCAGCCCCAGGATATGGCggg                                       >  5:444450/1‑136 (MQ=255)
                                                                                        ggatCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTTGCCCGGCTGGCCTTTTAGCAGGGCCAGTTTGCCGAGGTAGTTGAACCACTCAATAAGCAGCAGCCACAGGATATGGGGGAt                                      >  6:392607/4‑137 (MQ=255)
                                                                                        ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTATATATTCTGCCGCCCCCGGATATGGCGGAt                                      >  7:189860/1‑137 (MQ=255)
                                                                                        ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGGTGAAGAACTCTATATTCAGCAGCACCAGGATATGGCggct                                      >  6:246250/1‑135 (MQ=255)
                                                                                          cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCCCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGGTGGGCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                    >  4:213143/1‑137 (MQ=255)
                                                                                               gctggtTGCCAAAATACCGCACGCCGCGCCCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                               <  5:14464/137‑1 (MQ=255)
                                                                                                 tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                             >  8:414413/1‑137 (MQ=255)
                                                                                                                  cACGCCGCGCGCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa            <  8:14449/137‑1 (MQ=255)
                                                                                                                    cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTTCAGGGCCTGTTGTCCGAGGTTGTTGAAGAACTCAATATTCAGCAGCACCAGGATATGTGGGATTTCGTTGAACCAATTCTTGGACTCGACa          >  4:403057/1‑137 (MQ=255)
                                                                                                                    cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa          >  3:217447/1‑137 (MQ=255)
                                                                                                                    cgccgcGCTCCACGCCAAGCTCCGCCCGCCCTGTCTTGCATTTTTTCAGCGACTGTTGGCCGAGGTTGTAGCAGAACTCTATCTGCACCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa          <  8:169030/137‑1 (MQ=255)
                                                                                                                     gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAg         >  6:151875/1‑137 (MQ=255)
                                                                                                                     gccgcGCTCGAAGGCCAGGCGCGCCTGGCCCGGCAGGCCTTTTTGCAGTGCCTGTTGGCCGAGGTTGTTGAAGAACTCAATATGCAGCAGCAACAGGATATGGCGGTTTTAATTGACCCAATGCTTGGCCTCGACac         >  8:180814/1‑136 (MQ=255)
                                                                                                                          gcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc    >  7:361260/1‑137 (MQ=255)
                                                                                                                            tCGAAGGCCCGGCGCGCCTGGCCGGGCGGGGCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaa  >  8:47833/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                               
TTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAA  >  KT2440/2094675‑2094935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑