Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,856,8860TG53.6% ‑4.2 / 12.5 29S297A (TCG→GCG) hdfR_4HTH‑type transcriptional regulator HdfR
Reads supporting (aligned to +/- strand):  ref base T (1/12);  new base G (3/12);  total (5/24)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGC  >  KT2440/2856751‑2857013
                                                                                                                                       |                                                                                                                               
ccccgCGACCTGGTGGGCGAGCCGCTCATCAACGAGTAATTGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTCCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGGGTGGCCGCGGGCCGGCTc                                                                                                                                <  2:94651/133‑1 (MQ=255)
cccAGCGACCTGGTGGGCGAGCCGCTCAGCAACGAGGAAATGGTGGTGCTGGCCCGGCGGGGGCACCCGCTGTTGCACACTCACCTGCCGCTCAAGGGCGTGCACCCGGCGCGCTGGGTGTTGCCGCGGGCCGGCTc                                                                                                                                <  6:321676/136‑1 (MQ=255)
  cAGCGACCTGGTGGGCGAGCCTCACAACAACGAGGAAATCGTGGTGATGGCCCGTCGGGGGAACCAGCTCATGCACACTCACCTGACCCTCAAGGGCGTGCACCAGGCTCGCTGGGTGTTGCCGCGGGCCGGCTCGc                                                                                                                              >  7:274678/1‑137 (MQ=255)
  cAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGgcgcgc                                                                                                                              >  8:420107/1‑137 (MQ=255)
       aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGGGGGGCGCCGGcc                                                                                                                         >  2:356908/1‑137 (MQ=255)
       aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGGGGGGCGCCGGcc                                                                                                                         >  2:85140/1‑137 (MQ=255)
       aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCGGGGggccggcc                                                                                                                         >  8:113934/1‑137 (MQ=255)
           ggtggGCGAGCCGCTCATCACCGAGCAAATGGTGGTGCGGGCCCGTCGGGGGCACCCGCGGTTCCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTc                                                                                                                     <  7:94306/137‑1 (MQ=255)
                                  tggAAATGGTGGTGCTGGCCCGTGGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc                                                                                              <  8:366051/136‑1 (MQ=255)
                                  cgtaaaTGGTGGTGCGGGCCCGGCGGGGGCACCCGCTGGTCCACACTCACCTGCCGCTCAAGGGCGTGAACCAGGCGCGCTGGGTGTTGCGGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc                                                                                              <  6:452234/134‑1 (MQ=255)
                                  aGGAAATGGTGGTGCTGGCCCGGCGGGGGCACCCGCTGTTCCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc                                                                                              <  5:310846/137‑1 (MQ=255)
                                    aaaaGGGGGGTGCGGGCCCGGCGGGGGCACCCGCTGTTGCACACTCACCTGCCGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgccgc                                                                                            <  2:4420/136‑1 (MQ=255)
                                             gggCTGGCCGGGCGGGGGCCCCGGCTGTTGCACACTCACCTGACGCTCACGGGGGTGCACCCGGCGGGCGGGGTGTGCCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCt                                                                                   <  5:118230/135‑1 (MQ=255)
                                                      cGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGCGCCGGCACGCCGGCCCGTCAGCTGCTCGGCACCATCTGAGCAGCCCCGGGGCTGACCGCGcc                                                                          >  2:281293/1‑137 (MQ=255)
                                                        gcGGGGGCACCGGCTGTAGCCCACACACCTCCCCGACACGGGCGCCCCCCAGCGGGGGGGGGTGGGGCGGGGGGCGGGCGCGCCGCCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt                                                                        <  3:252375/136‑1 (MQ=255)
                                                        gcGGGGCCACCCGCTGTTCCCCCCGCCCCTCCCCCTCCCGGGCGTGAACCCGGCGCGCGGGGGGTCGCCCCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt                                                                        <  4:238242/136‑1 (MQ=255)
                                                          ggggcCCCCGGCTGTGCCCCACTCCCCTGCCGCGCCGGGGCGGGCACCGGGGGCGCGGGGGGTGGCGGGGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTgg                                                                      <  2:186530/137‑1 (MQ=255)
                                                                  ccGCTGTGGCACCCTCACCTGACGCTCAAGGGCGTGCACCAGGCGGGGTGGGTGTGGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCggtggt                                                              <  1:311623/137‑1 (MQ=255)
                                                                               cTCACCTGCCGCTCAGGGGGGGCCACACGGCGCGGGGGGTGTTCCGGCGGGCCGGCGCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGAt                                                 <  6:13444/137‑1 (MQ=255)
                                                                                    ccgccGCTCACGGGCGGCCACCTGGCGGGGGGGGTGTGGCGGGGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGc                                            <  4:371569/133‑1 (MQ=255)
                                                                                          cTCAAGGGCGTGCACCAGGCGCGGGGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                      <  6:398681/137‑1 (MQ=255)
                                                                                          cTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCGGGCGGGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                      <  8:288435/137‑1 (MQ=255)
                                                                                          cTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt                                      <  7:113934/137‑1 (MQ=255)
                                                                                            cAAGGGCGTGCACCAGGCGGGCTGGGTGTTGCCGGGGGCCGGCGCGCCGCCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATcc                                    <  3:430740/137‑1 (MQ=255)
                                                                                              cgggCGTGCACCAGGCGCGGTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGt                                  <  6:423045/136‑1 (MQ=255)
                                                                                                 gCGTGCACCCGGCGCGCTGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGc                               <  6:295669/137‑1 (MQ=255)
                                                                                                                  ggggTGGTGCCGGGGGCCGGCGGGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGa              <  4:130266/136‑1 (MQ=255)
                                                                                                                    ggTGTGGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACa            <  3:333484/137‑1 (MQ=255)
                                                                                                                              ggggcgggcTCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTggcggc  <  7:226768/136‑1 (MQ=255)
                                                                                                                              ggggCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTggcggc  <  1:356908/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                               
GCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGC  >  KT2440/2856751‑2857013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑