Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,350,3600TG37.7% 71.3 / 11.8 53R28R (CGT→CGG) CJ019_04090hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/27);  new base G (20/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350228‑4350495
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aGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGtttga                                                                                                                                      >  5:339349/1‑135 (MQ=255)
         aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGCCCGGGTGATCTACCTgg                                                                                                                             >  8:281076/1‑137 (MQ=255)
         aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGGTTTATCTACCTgg                                                                                                                             >  8:321079/1‑137 (MQ=255)
         aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGGTTCATATACCAgg                                                                                                                             >  6:208566/1‑137 (MQ=255)
           tGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGAt                                                                                                                           >  6:224308/1‑137 (MQ=255)
             tCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                         <  2:163733/137‑1 (MQ=255)
                   gATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑gg                                                                                                                  <  1:258927/137‑1 (MQ=255)
                   gATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑gg                                                                                                                  <  1:136530/137‑1 (MQ=255)
                     tCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGcc                                                                                                                <  7:119660/137‑1 (MQ=255)
                        gTGACTTGATCGAGGCCCCCCGCCCTTGCTTGACTGCCCGCTGCCGCGCACGCTGGACTACGTTGACCACAGTCAGCGGGGGCTGACCCGTCGGCGCTGGCGCGACCGTTTCTTCTACCTGGATGCCGAT‑GGCCAAc                                                                                                             <  6:45108/137‑1 (MQ=255)
                                   gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCCTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTAcgcgcc                                                                                                  >  8:214031/1‑135 (MQ=255)
                                   gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGTTTACTCAACCAGGATGCCCAT‑GGCCCACGGGGACGCGAc                                                                                                  >  7:32075/1‑137 (MQ=255)
                                     ggCCCGCCGCCCATGCTGGCCTGCCCCCGGCCGCGCACCCTGCACTACGTAGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACTGGTACGCGACAg                                                                                                <  7:403249/137‑1 (MQ=255)
                                      gcacgccgcCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGGTGCTCGACCGGGATGGCGAG‑GGCCCACAGGGGCGCGGCAGc                                                                                               >  7:172412/4‑137 (MQ=255)
                                        ccgccgCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTTGCTCTACCTGGATGCCGAT‑GGCCCACGGGTTCGCGCCcgcgc                                                                                             >  7:211162/1‑136 (MQ=255)
                                         cgccgcCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCCCGCGGTTCATATAACAGGATTGCCAT‑GGCCAACCCGTAAGGGACAGCgca                                                                                            >  2:79007/1‑135 (MQ=255)
                                                 aTGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTgc                                                                                    <  1:176191/137‑1 (MQ=255)
                                                      tGAGTGCCCGCTGCCGCGCACCCTGACGTACGTTGACGTGAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACGGTTTCTTCTACCTGGATGCCGTT‑CGACAACGGGTACGCGACAGCGAAACCCTTgcgcgct                                                                               <  3:372340/137‑2 (MQ=255)
                                                          tGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcgc                                                                           <  8:31788/137‑1 (MQ=255)
                                                           gCCCCCTGCCGCGCACCCTGCACTAGGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcgcc                                                                          <  6:48501/137‑1 (MQ=255)
                                                           gCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCGACCTGGATGACGCA‑GGGCAACGCGTTCGCGTCAGCGAACACCCTGCGCGCCTcccc                                                                          >  4:339874/1‑137 (MQ=255)
                                                               ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGGGGGGGCGCCCGGTTCATGGACCTGGGTGGCGCT‑GGGCCACGGGGACGCGCCCGCGCAACCCGTGCGCGCATcggcgccg                                                                      >  2:62162/1‑135 (MQ=255)
                                                               ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGCCCGTTTAATCTACCAGGATGCCGAT‑GGCCAACGGGTTCGCGACAGCGAAACCCTTGCGCGCAAGGCCGCaa                                                                      >  6:231259/1‑136 (MQ=255)
                                                                   ccGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGt                                                                  <  5:51872/137‑1 (MQ=255)
                                                                        cACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcc                                                             <  8:311532/137‑1 (MQ=255)
                                                                         aCCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTCATCTACCTGGATGCCGAG‑GGGCCAAGGGTACGCGCCAGCCAAAACCATGCCCGCAACGCCCCATTGGTgtaccc                                                            >  4:52252/1‑132 (MQ=255)
                                                                          cccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccc                                                           <  4:403256/137‑1 (MQ=255)
                                                                          cccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccc                                                           <  7:281076/137‑1 (MQ=255)
                                                                          cccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccc                                                           <  7:59505/137‑1 (MQ=255)
                                                                           ccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTAATCTACCTGGATGCCGAT‑TGCCAACGGGTACGCGACAGCGAAACCCATGCGCGCCTCGCCGCATTTGTGGTacccc                                                          >  1:54990/1‑137 (MQ=255)
                                                                            cTGCACTACGTTGACGACAGTCAGCGGGGCCTGACCCGTCGGCGCTGGCGCGACCTTTTCATCTACCTGGATGCCGAT‑GGCCAACGTGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                         <  1:79007/137‑1 (MQ=255)
                                                                                aCTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGGCCGGTTAATATACCTGGATGCCGAT‑TGCCAACCGGGACGCGCCAGCGAAACCCATTCGCCCCACGCCCCAATTGTGATCCCCCCGGcc                                                     >  4:296879/1‑137 (MQ=255)
                                                                                 cTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  7:129710/137‑1 (MQ=255)
                                                                                  tACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTCATCTTCCGGGATGCCGAT‑GGCCCACGGGTACGCGCCCGCGCAACCCTTGCGCGCCTCGCCGCCTTGGTGATCCCCCCGGCCTa                                                   >  4:281504/1‑137 (MQ=255)
                                                                                  tACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTAATCTACCTGGATTCCGAT‑GGCCAACCGGTACCCGCCAGCGCAACCCATTCGCCCAAAGCCGCAATGGTGGTACCCCCCGccga                                                   >  4:298289/1‑135 (MQ=255)
                                                                                    ggTTCACGACAGTCAGCGGGGCCTGACCCGGCGGCGGTGGCGCGACCGTTTCATCTACGTGGATCCCGAT‑GCCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  1:397634/136‑1 (MQ=255)
                                                                                    cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCGTaca                                                 <  7:242455/137‑1 (MQ=255)
                                                                                    cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  1:243985/137‑1 (MQ=255)
                                                                                    cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  7:440431/137‑1 (MQ=255)
                                                                                        gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGGCCGGTTGATCTACCTGGATTCCGAT‑GGCCCACCGGTAAGCGACAGCGAAACCCATGCGCGCATCGCCGCCTTGGTGATCCCCCCCGCCTACACGGa                                             >  2:1130/1‑137 (MQ=255)
                                                                                        gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGCCAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGa                                             >  2:81654/1‑137 (MQ=255)
                                                                                        gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCCGGCCCCGTTGATCTAACTGGAAGCCCAT‑GGCCAAACGGTACCGCCCCCCGAAACCCATGCCCCCCTCGCCCCCATGTTGGACCCCCCCGCCTACACCGa                                             >  8:440431/1‑137 (MQ=255)
                                                                                         acgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAt                                            <  6:339349/137‑1 (MQ=255)
                                                                                         acgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAt                                            <  5:224308/137‑1 (MQ=255)
                                                                                          cgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGGGACCGGTTGATCTACCTGGCTTCCGCTGGGCCACCGGGT‑CGCCACCGCGAAACCCATGCGCGCCACCGCGCATTGGGGATACCCCCCGCCAAAACGGAtg                                           >  8:230278/1‑137 (MQ=255)
                                                                                          cgacAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCCGTTAATCTACCATGCTTCCGCT‑TGGCAACGGGGTAGCGACCGCGAAACCCAGGCGCGCATCGCCCCATTTGTGATGCCCCCCGCCAACACGGAtg                                           >  8:264683/1‑137 (MQ=255)
                                                                                            acAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCGAACGGGCTTGCCAT‑GGCCCACCCGTACGCGCCACCGCAAACCATTCGCGCAACGCCGCATTGGTGATGCCCCCCGCCTACACgcaagtt                                         >  2:32330/1‑131 (MQ=255)
                                                                                                   gCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCt                                  <  4:197659/137‑1 (MQ=255)
                                                                                                   gCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCCGGGCATCGAACCGGCTGCCCCT‑GGCCCACGGGGACGCCCCCGCGCATCCCGTGCGCGCAAAGCCGGCGTGGTGGTGCCCCCGGCCTACCCGGGTGTGTGGGTcg                                  >  6:418563/1‑136 (MQ=255)
                                                                                                            gACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATc                         <  6:135435/137‑1 (MQ=255)
                                                                                                               ccGTCGGCGCGGTCGCGACCGGTTAATCTTACATGATTCCGAT‑TGCCCAACGGTAAGCGACAGCGCAACCCGATCGCGCATAGACGCATTGGTGCTAGCCCCGGCCTACACGGATGTGTGTGTCTTCGCCGCTACGc                      >  8:241217/1‑137 (MQ=255)
                                                                                                                 gTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCaa                    <  6:155092/137‑1 (MQ=255)
                                                                                                                  tCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAg                   <  8:32075/137‑1 (MQ=255)
                                                                                                                                   gTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCAcc  >  2:345523/1‑137 (MQ=255)
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AGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGAT‑GGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350228‑4350495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑