Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,384,2650CG42.0% 53.9 / 23.2 51intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (6/23);  new base G (21/0);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 5.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  KT2440/5384133‑5384395
                                                                                                                                    |                                                                                                                                    
gCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGtttt                                                                                                                                  >  4:195029/1‑137 (MQ=255)
                  aaTTAAATCAGAACACACGGCAAAGCATGTAGTACCGACAGCGGAGTACTGGCGGAGGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTgg                                                                                                               <  1:199684/136‑1 (MQ=255)
                                acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCggggagt                                                                                                 >  6:171523/1‑133 (MQ=255)
                                acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTAGTTTTTTTT‑GTCTGGAATTTTTGCGCTGCGGGTCG‑a                                                                                                >  6:129256/1‑137 (MQ=255)
                                     gCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTGTTTTTTT‑GGAGGGAATTTTGGCGCTGCGGGTCC‑taaaaa                                                                                           >  4:351437/1‑136 (MQ=255)
                                       tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTGTTTTTGT‑GGCGGGGAATTTGGAGCGGCGGGTGG‑AAAAATCg                                                                                         >  4:166673/1‑137 (MQ=255)
                                       tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGAATTTGGGGGCGGGGGGGGG‑AAAAAtag                                                                                         >  4:152004/1‑135 (MQ=255)
                                        aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGCTTTTTGGCGCTGCGGGGCG‑AAAAAtacg                                                                                        >  4:164821/1‑134 (MQ=255)
                                           cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCAGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGAtt                                                                                     <  4:167202/137‑1 (MQ=255)
                                            aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGT‑TGGGGGGATTTTGGGGGTGCGGGTGG‑GAAAATAGGAttt                                                                                    >  5:157305/1‑137 (MQ=255)
                                            aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TGCGGGTATTTTGTGGCTGGCGGGCG‑AAAAAAAGGAtat                                                                                    >  7:35575/1‑135 (MQ=255)
                                              gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTaa                                                                                  <  1:62451/137‑1 (MQ=255)
                                                cgtaCCGACAGCGGAGAACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAACACGCCCTGGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AATAATCGGATTTAAcc                                                                                <  6:382088/136‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                <  1:83489/137‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                <  1:57143/137‑1 (MQ=255)
                                                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                <  6:273060/137‑1 (MQ=255)
                                                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAccc                                                                               <  6:455050/137‑1 (MQ=255)
                                                   aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGAAATTGGGCGCTGCGGGTCG‑AAAAAACGCATTTAAcccca                                                                             >  7:341004/1‑136 (MQ=255)
                                                       aCAGCGGAGTACTGGCGGATGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GCCGGGAATTTTTGCGCGGCGGGTGGAAAAAAACGGTTTTAACCCGttct                                                                          >  5:318211/1‑135 (MQ=255)
                                                       aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑TCCGGGAATTTGGGGGCTGGGGGTGG‑AAAAATCGGATTTTAACCCGTTCt                                                                         >  6:151925/1‑137 (MQ=255)
                                                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTT‑TGCTGGAATTTTGGCGCGGCGGGTCG‑AAAAAAAGGATTTAACACCattatt                                                                        >  2:9615/1‑137 (MQ=255)
                                                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCGCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TCCGGGGATTTTGGGGGGGCGGGGGG‑GAAAATCGGGTTTTACCCCattagt                                                                        >  6:414152/1‑134 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTT‑GGCTGGGATTTTGGGGCTGGGGGGGG‑AAAAATCGGATTTTAACCCTGtgttgt                                                                      >  8:136741/1‑137 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GCCGGGCATTTGGGCGCTGCGGGGGG‑AAAAATAGGATTTAACCCCGTTCTTGt                                                                      >  8:133874/1‑137 (MQ=255)
                                                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCATCGGGCGTCGTTTTTTTT‑GCCTGGTATTTTTGGGCTGCTGGTGG‑AAATATCGGATTTAAAACCTTtgttgt                                                                      >  6:348506/1‑137 (MQ=255)
                                                            ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TGCGGGTATTTTGGGGCTGCGGGGGG‑AAAAATAGGGTTTTACCCCATtttttttt                                                                    >  5:394458/1‑133 (MQ=255)
                                                              aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTT‑GGGGGGTATTTTGGGGGGGCGGGGGG‑AAAAAAAGGATTTTAACCCATtgttgttgag                                                                  >  8:391117/1‑133 (MQ=255)
                                                                tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                <  3:104436/137‑1 (MQ=255)
                                                                 aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGTATTTGGGGGCGGCGGGGGG‑GAAAAACGGATTTAACCCCATTATTGTTgggggg                                                               >  8:264416/1‑134 (MQ=255)
                                                                    ggCGGACGGGGGTTCAAATCCCCCGGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  1:345131/137‑1 (MQ=255)
                                                                      cGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGTATTTGGGGGGTGCGGGGGG‑GAAAATAGGATTTTACCCCTTTCTTGTAGGGGGTTTgcc                                                          >  4:164727/1‑137 (MQ=255)
                                                                        gACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGGATTTGGGGGCTGGGGGGGG‑CAAAAGTGGGTTTTACACCGTTCGTGTTGGGGGGTTGCCac                                                        >  7:60742/1‑136 (MQ=255)
                                                                          ggggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                      <  2:351592/136‑1 (MQ=255)
                                                                          cGGGGGTTCTAATCCCACCTGCTCCACCAAATACAAAGATGAAGACGCCCTGGGGCGTCTTTTTTTGT‑GCCTGGAATTTTGGCGCTGCGTGTCG‑AAAAATCTGATTTAACCCCTTTCTTGTAGGGCGTTTCCGAAAg                                                      <  6:374545/137‑1 (MQ=255)
                                                                           gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGGCGTCTAATTTTTCCG‑TGAGGGTCG‑TAAAATACGATTTAAACCCTGTATTTTATAGGGTTTCGCaaaaa                                                     >  4:301028/1‑135 (MQ=255)
                                                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGGGGGGATTTTGGGGGGGGGGGGGG‑GAAAATGGGGTTTTAACCCCTTGTTGTAGGGGGGTTGCCAAAGAtt                                                   >  2:252071/1‑136 (MQ=255)
                                                                                 tCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                               <  1:252071/137‑1 (MQ=255)
                                                                                 tCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGAATTAACCCCTTTCTTGTAGCGCGTTTCCCAAAGATActgc                                               <  7:326672/137‑1 (MQ=255)
                                                                                                  caccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTT‑TGCTGGAATTTTGGCGGTGCGGGGCG‑AAAAATCGGATTTAACCCCTGTCTTGTAGGGCGTTTGCCAAAGATAAATCATGCGCCTGTTTTCCGt                              >  6:407272/1‑137 (MQ=255)
                                                                                                     caAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTT‑GGGTGGGATTTTGGGGCGGGGGGGGG‑CAAAATAGGTTTTTAACCCGTTGTTGTTGGGGGTGTTCCAAATATACTGACGGGGCCGGTTTTGCGTTgg                           >  8:41569/1‑136 (MQ=255)
                                                                                                     caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGc                           <  3:411417/137‑1 (MQ=255)
                                                                                                      aaaTCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGTATTTGGGCGCTGCGGGGGG‑AAAAATAGGGTTTAACCCCTTTCTTGTTGGGCGGTTTGCAAAGATAATGCCTGCGCCGGTTTTACGTTggg                          >  2:66623/1‑135 (MQ=255)
                                                                                                       aaTCGAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTCGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCGCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                         <  5:129256/137‑1 (MQ=255)
                                                                                                       aaTCCAAAGAATAAGACGCCCTCGGGCGTCTGTTTTTGT‑TGCGTGAATTTGGGCGGTGCGGGGCG‑AAAAATAGGTTTTAACCACTTTCTTTTAGGGCGTGTGCCCAACAAAAAGCCTGCCCCGGGTTTCCGTTGCgg                         >  4:351514/1‑137 (MQ=255)
                                                                                                       aaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                         <  1:45576/137‑1 (MQ=255)
                                                                                                               taagaagaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCTGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                 <  3:103547/136‑1 (MQ=255)
                                                                                                                aagaagaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGAGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAtttttt                <  7:391117/137‑1 (MQ=255)
                                                                                                                    agaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg            <  3:195029/137‑1 (MQ=255)
                                                                                                                    agaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg            <  1:9615/137‑1 (MQ=255)
                                                                                                                       cGCCCTCGGGCGTCTTTTTTTGT‑GACTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtt         <  4:35228/137‑1 (MQ=255)
                                                                                                                              gggCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct  <  7:136741/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  KT2440/5384133‑5384395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑