Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,384,2890CG27.5% 84.6 / 11.3 52intergenic (+552/‑306)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (12/25);  new base G (14/0);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.09e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACG  >  KT2440/5384165‑5384418
                                                                                                                             |                                                                                                                                  
acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCggggagt                                                                                                                        >  6:171523/1‑133 (MQ=255)
acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTAGTTTTTTTT‑GTCTGGAATTTTTGCGCTGCGGGTCG‑a                                                                                                                       >  6:129256/1‑137 (MQ=255)
     gCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCCCCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTGTTTTTTT‑GGAGGGAATTTTGGCGCTGCGGGTCC‑taaaaa                                                                                                                  >  4:351437/1‑136 (MQ=255)
       tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTGTTTTTGT‑GGCGGGGAATTTGGAGCGGCGGGTGG‑AAAAATCg                                                                                                                >  4:166673/1‑137 (MQ=255)
       tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGAATTTGGGGGCGGGGGGGGG‑AAAAAtag                                                                                                                >  4:152004/1‑135 (MQ=255)
        aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGCTTTTTGGCGCTGCGGGGCG‑AAAAAtacg                                                                                                               >  4:164821/1‑134 (MQ=255)
           cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCAGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGAtt                                                                                                            <  4:167202/137‑1 (MQ=255)
            aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGT‑TGGGGGGATTTTGGGGGTGCGGGTGG‑GAAAATAGGAttt                                                                                                           >  5:157305/1‑137 (MQ=255)
            aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TGCGGGTATTTTGTGGCTGGCGGGCG‑AAAAAAAGGAtat                                                                                                           >  7:35575/1‑135 (MQ=255)
              gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTaa                                                                                                         <  1:62451/137‑1 (MQ=255)
                cgtaCCGACAGCGGAGAACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAACACGCCCTGGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AATAATCGGATTTAAcc                                                                                                       <  6:382088/136‑1 (MQ=255)
                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                                       <  1:83489/137‑1 (MQ=255)
                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                                       <  1:57143/137‑1 (MQ=255)
                agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAcc                                                                                                       <  6:273060/137‑1 (MQ=255)
                 gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAAccc                                                                                                      <  6:455050/137‑1 (MQ=255)
                   aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGAAATTGGGCGCTGCGGGTCG‑AAAAAACGCATTTAAcccca                                                                                                    >  7:341004/1‑136 (MQ=255)
                       aCAGCGGAGTACTGGCGGATGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GCCGGGAATTTTTGCGCGGCGGGTGGAAAAAAACGGTTTTAACCCGttct                                                                                                 >  5:318211/1‑135 (MQ=255)
                       aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑TCCGGGAATTTGGGGGCTGGGGGTGG‑AAAAATCGGATTTTAACCCGTTCt                                                                                                >  6:151925/1‑137 (MQ=255)
                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTT‑TGCTGGAATTTTGGCGCGGCGGGTCG‑AAAAAAAGGATTTAACACCattatt                                                                                               >  2:9615/1‑137 (MQ=255)
                        cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCGCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TCCGGGGATTTTGGGGGGGCGGGGGG‑GAAAATCGGGTTTTACCCCattagt                                                                                               >  6:414152/1‑134 (MQ=255)
                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTT‑GGCTGGGATTTTGGGGCTGGGGGGGG‑AAAAATCGGATTTTAACCCTGtgttgt                                                                                             >  8:136741/1‑137 (MQ=255)
                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GCCGGGCATTTGGGCGCTGCGGGGGG‑AAAAATAGGATTTAACCCCGTTCTTGt                                                                                             >  8:133874/1‑137 (MQ=255)
                          gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCATCGGGCGTCGTTTTTTTT‑GCCTGGTATTTTTGGGCTGCTGGTGG‑AAATATCGGATTTAAAACCTTtgttgt                                                                                             >  6:348506/1‑137 (MQ=255)
                            ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑TGCGGGTATTTTGGGGCTGCGGGGGG‑AAAAATAGGGTTTTACCCCATtttttttt                                                                                           >  5:394458/1‑133 (MQ=255)
                              aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTT‑GGGGGGTATTTTGGGGGGGCGGGGGG‑AAAAAAAGGATTTTAACCCATtgttgttgag                                                                                         >  8:391117/1‑133 (MQ=255)
                                tACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCg                                                                                       <  3:104436/137‑1 (MQ=255)
                                 aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGCGGGTATTTGGGGGCGGCGGGGGG‑GAAAAACGGATTTAACCCCATTATTGTTgggggg                                                                                      >  8:264416/1‑134 (MQ=255)
                                    ggCGGACGGGGGTTCAAATCCCCCGGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                                   <  1:345131/137‑1 (MQ=255)
                                      cGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGTATTTGGGGGGTGCGGGGGG‑GAAAATAGGATTTTACCCCTTTCTTGTAGGGGGTTTgcc                                                                                 >  4:164727/1‑137 (MQ=255)
                                        gACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGGATTTGGGGGCTGGGGGGGG‑CAAAAGTGGGTTTTACACCGTTCGTGTTGGGGGGTTGCCac                                                                               >  7:60742/1‑136 (MQ=255)
                                          ggggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                                             <  2:351592/136‑1 (MQ=255)
                                          cGGGGGTTCTAATCCCACCTGCTCCACCAAATACAAAGATGAAGACGCCCTGGGGCGTCTTTTTTTGT‑GCCTGGAATTTTGGCGCTGCGTGTCG‑AAAAATCTGATTTAACCCCTTTCTTGTAGGGCGTTTCCGAAAg                                                                             <  6:374545/137‑1 (MQ=255)
                                           gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGGCGTCTAATTTTTCCG‑TGAGGGTCG‑TAAAATACGATTTAAACCCTGTATTTTATAGGGTTTCGCaaaaa                                                                            >  4:301028/1‑135 (MQ=255)
                                             gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTT‑GGGGGGGATTTTGGGGGGGGGGGGGG‑GAAAATGGGGTTTTAACCCCTTGTTGTAGGGGGGTTGCCAAAGAtt                                                                          >  2:252071/1‑136 (MQ=255)
                                                 tCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                                                      <  1:252071/137‑1 (MQ=255)
                                                 tCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGAATTAACCCCTTTCTTGTAGCGCGTTTCCCAAAGATActgc                                                                      <  7:326672/137‑1 (MQ=255)
                                                                  caccaAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTT‑TGCTGGAATTTTGGCGGTGCGGGGCG‑AAAAATCGGATTTAACCCCTGTCTTGTAGGGCGTTTGCCAAAGATAAATCATGCGCCTGTTTTCCGt                                                     >  6:407272/1‑137 (MQ=255)
                                                                     caAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTT‑GGGTGGGATTTTGGGGCGGGGGGGGG‑CAAAATAGGTTTTTAACCCGTTGTTGTTGGGGGTGTTCCAAATATACTGACGGGGCCGGTTTTGCGTTgg                                                  >  8:41569/1‑136 (MQ=255)
                                                                     caAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGc                                                  <  3:411417/137‑1 (MQ=255)
                                                                      aaaTCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGT‑GGCTGGTATTTGGGCGCTGCGGGGGG‑AAAAATAGGGTTTAACCCCTTTCTTGTTGGGCGGTTTGCAAAGATAATGCCTGCGCCGGTTTTACGTTggg                                                 >  2:66623/1‑135 (MQ=255)
                                                                       aaTCGAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTCGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCGCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                                <  5:129256/137‑1 (MQ=255)
                                                                       aaTCCAAAGAATAAGACGCCCTCGGGCGTCTGTTTTTGT‑TGCGTGAATTTGGGCGGTGCGGGGCG‑AAAAATAGGTTTTAACCACTTTCTTTTAGGGCGTGTGCCCAACAAAAAGCCTGCCCCGGGTTTCCGTTGCgg                                                >  4:351514/1‑137 (MQ=255)
                                                                       aaTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                                <  1:45576/137‑1 (MQ=255)
                                                                               taagaagaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCTGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                                        <  3:103547/136‑1 (MQ=255)
                                                                                aagaagaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGAGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAtttttt                                       <  7:391117/137‑1 (MQ=255)
                                                                                    agaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                                   <  1:9615/137‑1 (MQ=255)
                                                                                    agaCGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                                   <  3:195029/137‑1 (MQ=255)
                                                                                       cGCCCTCGGGCGTCTTTTTTTGT‑GACTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtt                                <  4:35228/137‑1 (MQ=255)
                                                                                              gggCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct                         <  7:136741/137‑1 (MQ=255)
                                                                                                     tttttttGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg                  <  8:286205/137‑1 (MQ=255)
                                                                                                        ttttGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg               <  6:157305/137‑1 (MQ=255)
                                                                                                                      aTTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACg  <  3:68742/137‑1 (MQ=255)
                                                                                                                             |                                                                                                                                  
ACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGT‑GCCTGGAATTTGGGCGCTGCGGGTCG‑AAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACG  >  KT2440/5384165‑5384418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑