breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 3,793,677 (T)7→6 coding (649/699 nt) rfaY ← lipopolysaccharide core heptose(II) kinase RfaY

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 274995–275641 286981–286346 10706–11987 65 [64] [64] 65 [BW25113_RS01350]–[BW25113_RS01405] [BW25113_RS01350],BW25113_RS01350,BW25113_RS23265,BW25113_RS01360,yagB,BW25113_RS26050,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,BW25113_RS23270,BW25113_RS01405,[BW25113_RS01405]
* * ÷ NZ_CP009273 387279–388427 397243 8817–9965 65 [63] [1] 114 [BW25113_RS01920]–[phoA] [BW25113_RS01920],BW25113_RS01920,BW25113_RS01930,iprA,ampH,sbmA,yaiW,yaiY,yaiZ,ddlA,BW25113_RS25915,iraP,[phoA]
* * ÷ NZ_CP009273 3629457 3646466–3645390 15934–17010 132 [0] [63] 65 yhiN–[BW25113_RS18160] yhiN,pitA,uspB,uspA,BW25113_RS26225,BW25113_RS26230,dtpB,rsmJ,prlC,rlmJ,gorA,dinQ,arsR,arsB,arsC,BW25113_RS18155,[BW25113_RS18160]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 269666 =NA (NA)139 (1.220) 114/496 0.0 98.6% intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
?NZ_CP009273 = 651895 2 (0.020)intergenic (‑471/‑118) dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
* ? NZ_CP009273 360104 =58 (0.510)77 (0.680) 65/496 1.9 55.8% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 64 (0.560)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 388422NA (NA)110 (0.970) 93/494 0.3 99.1% intergenic (‑65/‑7) BW25113_RS01920/BW25113_RS01930 IS3‑like element IS3 family transposase/autotransporter outer membrane beta‑barrel domain‑containing protein
?NZ_CP009273 397244 = 1 (0.010)coding (42/1416 nt) phoA alkaline phosphatase
* ? NZ_CP009273 651892 =2 (0.020)116 (1.020) 107/496 0.1 98.3% intergenic (‑468/‑121) dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
?NZ_CP009273 = 684501 NA (NA)intergenic (‑68/+298) BW25113_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
* ? NZ_CP009273 1203246 =10 (0.090)85 (0.800) 77/464 0.7 87.9% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 14 (0.130)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326110 (0.090)96 (0.900) 88/464 0.3 89.2% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 14 (0.130)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 36294560 (0.000)128 (1.130) 102/494 0.1 100% intergenic (+203/+112) yhiM/yhiN DUF2776 domain‑containing protein/NAD(P)/FAD‑dependent oxidoreductase
?NZ_CP009273 3645397 = NA (NA)pseudogene (747/747 nt) BW25113_RS18155 hypothetical protein