Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,036,390	0	A	G	39.2%	80.0 / 10.3	51	N206D (AAT→GAT)	srkA	stress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base A (18/13);  new base G (20/0);  total (38/13)
Fisher's exact test for biased strand distribution p-value = 6.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCC  >  NZ_CP009273/4036145‑4036637                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                             cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGCGACATGGA‑GACTGGGCAGCCGGGGata‑t                                                                                                                                                                                                                                                         >  1:426767/1‑250 (MQ=255)                tAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTTTGCGGCTTCCAGGG‑GACTGCCCCCCCGGGGATA‑TTTTTTGGGGC‑GGTgg                                                                                                                                                                                                                                         >  1:402300/1‑250 (MQ=255)                                    gggCGCAAACAGATTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCACGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGCAGCATTCACCGATCTGCGGCTACATGGC‑GACTGCCACGCCGGGAATC‑TTCTCTCGCGC‑GGTGGTGCCA‑TGTTTGTTGATCTgt                                                                                                                                                                                                                    >  1:397077/1‑249 (MQ=255)                                         cAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGG‑GACTGCCACGCCGGGGATA‑TTTTCTGGGGC‑GGTGGTCCAA‑TGTTTTTTTATCTGggtggt                                                                                                                                                                                                               >  1:146089/1‑248 (MQ=255)                                             cAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTTCCGTTCTGCGGCTCCCTGGA‑GACTGCCACGCCGGGGATA‑TTTTCTTGCGC‑GGTGGTCCCA‑TGTTTGTTGTTCTGGATGGTGCAc                                                                                                                                                                                                           >  2:291274/1‑250 (MQ=255)                                                cTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTCCCGTTCTGCGGCTACATGGG‑GGCTGCCACGCCGGGGATA‑TTTTTTGTCGC‑GATGGGCCAA‑CGTTTGTTGTTCTGGGTGGTGCACGt                                                                                                                                                                                                         >  2:39374/1‑249 (MQ=255)                                                  ttttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGGATA‑TTCTCTGGCGC‑GGTGGTCCCA‑TGTTTTTTGTTCTGGgtgatggcagtaat                                                                                                                                                                                                      >  1:47265/1‑242 (MQ=255)                                                        ccATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAGTA‑TTCTCTGGCGC‑GATGGTCCCA‑TGTTTGTTGATCTGGATGTTGCACGTAATGGtt                                                                                                                                                                                                  >  1:304093/1‑247 (MQ=255)                                                          aTCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGTGGGTGCCTGGG‑GACGGCCCACCCGGGAGAG‑TTTTCTGGCGCAGAGGGGCAAA‑T‑TTTATTGGTCTGGTTGATGCACGTAAAggggccgc                                                                                                                                                                                              >  2:152696/1‑244 (MQ=255)                                                                cGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGG‑GACTGCCACGCCGGGGATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGTTCTGGGTGATGCACGGAATGGTCCAGCCGtt                                                                                                                                                                                          >  1:89314/1‑248 (MQ=255)                                                                      tCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTTACCGGTCTGCGGCCACATGGA‑GACTGCCCCGCCGGGAAAA‑TTCTCTGGCGG‑GGTGGTGCCA‑TGTTTGTTGATCTGGGTGATGCACGTAATGGGCTCGCCGTGCTGGAgtt                                                                                                                                                                                  >  2:366510/1‑250 (MQ=255)                                                                               aCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCTCCATTGAGGACT‑CCCCGCCGGGGAAA‑TTTTCTTGCGC‑GCTTGGCCAATTTTTTTTTGTTTTGGTTG‑TGCAACTTATGGTCCAGCCGGTCCGGGTTTTTgggtttt                                                                                                                                                                         >  2:305065/1‑245 (MQ=255)                                                                               aCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCACATTGA‑GACTGCCACGCCGGGAATA‑TTTTCTTGCGC‑GGTGGTCCCA‑TGTTTTTTTGTCTGGTTGATTCACGTAATTGTCCCGCCGTTCTGGATTTGTGGGTGtt                                                                                                                                                                         >  1:814695/1‑250 (MQ=255)                                                                                     atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGGTACATGGA‑GACTGCCACGCCGGGGATA‑TTTTCTGGCGC‑GATGGTCCAA‑TGGTTGTTGGTCTGGGTGATGGCCGGAATGGGCGCGCCGGTCCGGGTTTGTGGGTGTTggt                                                                                                                                                                      >  2:253518/1‑245 (MQ=255)                                                                                     atCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAAGTTTACCGTTCTGCGGCTACATGGG‑GAATGCCACCCCCGGGAAA‑TTCTCTGGCGC‑GGTGGGTCAA‑TGGTTGTTGATCTTGGTGGTGCACGTAATGTTCCAGCCGGTTCGGATTTTTGGGTGTTGCTcca                                                                                                                                                                   >  2:406736/1‑248 (MQ=255)                                                                                                           gTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGGTACATGGA‑GAATGCCACGCCGGGGAAA‑TTTTCTGGCGC‑GGTGGTCCCA‑TGTTTGTTGATCTGGGTGATGCCAGGAATGGGCCAGCCGTTCAGGGTTTGTGGATGTTGGTCCATGGGGGTAAAGCCGAGGAGC‑gg                                                                                                                                            >  2:115/1‑250 (MQ=255)                                                                                                             ttGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGA‑GAATGCCACGCCGGGGATA‑TTCTCTGGCGC‑GGTGGGCCAC‑AGTTTGTTGTTCTGGATGATGCACGTAATGGTCCAGACGTTCAGGATTTTTGGGTGTTGCTCAATGGGGGTAAAGGCGAGCAGC‑GGAt                                                                                                                                          >  1:657870/1‑250 (MQ=255)                                                                                                               gAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTTTGCGGGTCCACGGA‑GACTGCCACCCCGGGGATATTTTTCTGG‑GC‑GATGGGCCCA‑TGTTTGTTTGTCTTGGTGGGGCACGTACTTGTCCAGCCGTTCCGGGTTTGTGGGTGTTGCTTAAATGCGGTAAAGACggggcagggggtc                                                                                                                                         >  2:107088/1‑238 (MQ=255)                                                                                                                     gctacccgaaTACCTTCCGGTTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTgg                                                                                                                                  <  2:426767/241‑1 (MQ=255)                                                                                                                                 ccTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTg                                                                                                                      <  1:384986/250‑1 (MQ=255)                                                                                                                                  cTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCTACATGGA‑GACTGCCACCCCGGGGATA‑TTCTCTTGCGC‑GATGGTCCCA‑TGTTTTTTGATCTGGATGATGCACGGAATTGTCCAGCCGTTCCGGGTTTTTGGTTGTTGTTCCATGGCGATAAAGACGGGCCGC‑GGGTGCAACTGGTAACTatttttt                                                                                                                      >  2:7771/1‑248 (MQ=255)                                                                                                                                       gggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGCTCCGCGGCTACATGGG‑GACTGCCCCCCCGGGGAAA‑TTTTCTGGCGC‑GTGGGGTCAA‑AGTTTTTTGTTCTGGGTGAAGTACGTAATGGTTCCGGCGGTCAGGGTTTTTGGGTGTTGCGTAAAGGGGATAAAAACGGGCAGG‑GGAGGGAAACGGGAACTATTATTTAAGG‑tt                                                                                                               >  2:11444/1‑250 (MQ=255)                                                                                                                                        ggTTGAAGACGGCTTGCCAGAAAGCGGCGAATGAGCTGAGTGACGCCGTTACAGCGATCGGGCGGGAAGATTTCACCGTTCTGCGGCTACAGGGG‑GGCTGCCAGGCAGGGAATA‑TGCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGAGGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTa                                                                                                              <  2:128533/250‑1 (MQ=255)                                                                                                                                                       tCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACCTTGG‑GACTGCCACGCCGGGGATA‑TTCTCTTGCGC‑GATGGTCCAA‑TGTTTTTTGGTCTTGGTGATGCACGTAATTGTCCAGCCGTTCAGGATTTGTGGATTTTTGTCAATGGCGGTAAAGCCGAGCAGC‑GGGTGCAACTGGGAACTATTTTTTAAGC‑TTATGAAGAATTTAGCgg                                                                                               >  1:778002/1‑249 (MQ=255)                                                                                                                                                          tGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGA‑GACTGCCCCCCCCGGGATA‑TTTTCTGGCGC‑GATGGTGCCA‑AGTTTTTTTATTTTGGTGATGCACGGAATTGTCCCGCCGTTTCGGATTTTTGGGTTTTTCTCCATGGGGGTTAAACCCGGCCGCGGGATCAAATTGGAAATTTTTTTTTAAGT‑TTATAAAAATTTTatgggtt                                                                                             >  2:210156/1‑244 (MQ=255)                                                                                                                                                           gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCTCCGTTCTGCGGCTACCTGGA‑GACTGCCACGCCGGGGATA‑TTCTCTGGCGC‑GATGGGCCCA‑TGTTTGTTGATCTGGATGATGGACGTAATGGTCCCGCCGGTCCGGATTTTTGGATGTTGTTCAATGGCGATAAAGCCCAGCAGC‑GGGTGCGAATGGAGACCATTATTTAAGC‑TTATGAAGAATTTAGCGAGttt                                                                                           >  2:806361/1‑249 (MQ=255)                                                                                                                                                            aaaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGA‑GACTGCCACGCCGGGGATA‑TTTTCTTGCGC‑GGTGGGCCAA‑TGTTTGTTTGTCTGGGTGATGGACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGTTCAAAGGCGATAAAGCCGAGCAAC‑GGATGCAACTGGGAAATATTATTGAAGC‑TTATTAAAGAtttttaggggtc                                                                                           >  2:199006/1‑240 (MQ=255)                                                                                                                                                            aaaGAGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGtt                                                                                            <  1:468702/248‑1 (MQ=255)                                                                                                                                                              aGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGac                                                                                        <  1:681612/250‑1 (MQ=255)                                                                                                                                                                      aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTGGGCTACATGGA‑GACTGCCCCGCCCGGGATA‑TTTTCTGGCGC‑GATGGGCCCA‑TGTTTTTTGTTCTTGGTGATGCACGTAATGGTCCAGCCGTTCCGGATTTGTGGGTGTTGTTCCATGGCGGTTAAGCCGAGCAGC‑GGGTGGAACTGGGAACTATTTTTTTAGCTTTTTGAAAAATTT‑GTGAGTTTGACACCCCTg                                                                                 >  2:584410/1‑249 (MQ=255)                                                                                                                                                                           cTGGTTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCg                                                                           <  2:624267/250‑1 (MQ=255)                                                                                                                                                                                                  cTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACgcgc                                                     >  1:621810/1‑249 (MQ=255)                                                                                                                                                                                                      cGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGc                                                <  1:690250/250‑1 (MQ=255)                                                                                                                                                                                                       gggAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCg                                               <  2:251177/250‑1 (MQ=255)                                                                                                                                                                                                          aaGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGttt                                            >  2:43767/1‑250 (MQ=255)                                                                                                                                                                                                            gATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg                                          >  2:716376/1‑250 (MQ=255)                                                                                                                                                                                                            gATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg                                          >  1:366158/1‑250 (MQ=255)                                                                                                                                                                                                                   ccGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttatta                                   >  2:394913/1‑250 (MQ=255)                                                                                                                                                                                                                     gTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATc                                 >  1:387080/1‑250 (MQ=255)                                                                                                                                                                                                                        cTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGACGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTg                              >  2:828472/1‑250 (MQ=255)                                                                                                                                                                                                                        cTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCtt                               <  1:332084/249‑1 (MQ=255)                                                                                                                                                                                                                         tGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGc                             >  1:536343/1‑250 (MQ=255)                                                                                                                                                                                                                                aCATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCt                       >  2:496325/1‑249 (MQ=255)                                                                                                                                                                                                                                         aCTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgcggcg              <  2:454538/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTgg          <  1:115/250‑1 (MQ=255)                                                                                                                                                                                                                                              cACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTggg         >  2:70748/1‑250 (MQ=255)                                                                                                                                                                                                                                                cGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCt       <  1:719855/250‑1 (MQ=255)                                                                                                                                                                                                                                                   cGGGAATA‑TTCTCTGGCGC‑GATGGTTCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGAGTTGGGCTGAt    >  2:374337/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGAt    >  1:250651/1‑250 (MQ=255)                                                                                                                                                                                                                                                    gggAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATc   <  2:89314/250‑1 (MQ=255)                                                                                                                                                                                                                                                     ggAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATcc  >  1:31246/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                             CGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGA‑GACTGCCACGCCGGGAATA‑TTCTCTGGCGC‑GATGGTCCAA‑TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGC‑GGATGCAACTGGAAACTATTATTGAAGC‑TTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCC  >  NZ_CP009273/4036145‑4036637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑