breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 121,211 Δ1 bp coding (1708/2664 nt) aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 274976–275642 286962–286347 10706–11987 46 [44] [45] 46 [BW25113_RS01350]–[BW25113_RS01405] [BW25113_RS01350],BW25113_RS01350,BW25113_RS23265,BW25113_RS01360,yagB,BW25113_RS26050,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,BW25113_RS23270,BW25113_RS01405,[BW25113_RS01405]
* * ÷ NZ_CP009273 387279–388428 397243 8816–9965 46 [45] [0] 96 [BW25113_RS01920]–[phoA] [BW25113_RS01920],BW25113_RS01920,BW25113_RS01930,iprA,ampH,sbmA,yaiW,yaiY,yaiZ,ddlA,BW25113_RS25915,iraP,[phoA]
* * ÷ NZ_CP009273 651593 651927 335 105 [0] [1] 97 dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
* * ÷ NZ_CP009273 3793508 3793685 178 95 [0] [0] 92 [waaZ]–[rfaY] [waaZ],[rfaY]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 269666 =NA (NA)105 (1.210) 85/496 0.1 100% intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
?NZ_CP009273 = 651592 0 (0.000)intergenic (‑168/‑421) dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
* ? NZ_CP009273 360104 =31 (0.360)61 (0.700) 55/496 1.3 61.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 47 (0.540)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 388422NA (NA)93 (1.070) 84/494 0.1 100% intergenic (‑65/‑7) BW25113_RS01920/BW25113_RS01930 IS3‑like element IS3 family transposase/autotransporter outer membrane beta‑barrel domain‑containing protein
?NZ_CP009273 397244 = 0 (0.000)coding (42/1416 nt) phoA alkaline phosphatase
* ? NZ_CP009273 651928 =1 (0.010)96 (1.100) 84/496 0.1 99.0% intergenic (‑504/‑85) dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
?NZ_CP009273 = 684501 NA (NA)intergenic (‑68/+298) BW25113_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
* ? NZ_CP009273 1203246 =9 (0.100)73 (0.900) 69/464 0.3 88.8% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 10 (0.120)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032619 (0.100)62 (0.760) 58/464 0.8 87.1% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 10 (0.120)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 2282398 =NA (NA)90 (1.030) 83/496 0.1 100% pseudogene (3/2532 nt) yejO autotransporter YejO
?NZ_CP009273 3793686 = 0 (0.000)coding (640/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY
* ? NZ_CP009273 = 3646590NA (NA)95 (1.090) 83/496 0.1 100% pseudogene (3/379 nt) BW25113_RS18165 hypothetical protein
?NZ_CP009273 = 3793507 0 (0.000)coding (50/852 nt) waaZ 3‑deoxy‑D‑manno‑oct‑2‑ulosonate III transferase WaaZ