BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,327,818	Δ1 bp	coding (2514/2598 nt)	topA →	type I DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,327,817	0	A	.	99.0%	905.2 / 1.9	190	coding (2513/2598 nt)	topA	type I DNA topoisomerase
Reads supporting (aligned to +/- strand): ref base A (0/2); new base . (95/93); total (95/95)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 193 total aligned reads displayed.

CGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAG  >  NZ_CP009273/1327568‑1328062

  gTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCata                                                                                                                                                                                                                                                     <  1:602547/250‑3 (MQ=255)

      cATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGtc                                                                                                                                                                                                                                                <  1:1189756/250‑1 (MQ=255)

       aTTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGtct                                                                                                                                                                                                                                               >  1:270540/1‑250 (MQ=255)

            cTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCg                                                                                                                                                                                                                                          >  1:861112/1‑250 (MQ=255)

               ggtgaccGTGCGAAATATCCGATGCTTATATCGGGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGGGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGaa                                                                                                                                                                                                                                       <  2:1130468/245‑1 (MQ=255)

               aGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGa                                                                                                                                                                                                                                        <  1:534192/249‑1 (MQ=255)

                gCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGaaa                                                                                                                                                                                                                                      <  1:313313/250‑1 (MQ=255)

                 cTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGaaaa                                                                                                                                                                                                                                     <  1:410069/250‑1 (MQ=255)

                    ccGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAg                                                                                                                                                                                                                                   <  1:1410497/249‑1 (MQ=255)

                           aaaaaTCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGCGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAg                                                                                                                                                                                                                           >  1:741213/1‑250 (MQ=255)

                             aaaTCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGTGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGc                                                                                                                                                                                                                         >  2:306078/1‑250 (MQ=255)

                              aaTCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCg                                                                                                                                                                                                                        <  1:817973/250‑1 (MQ=255)

                                tCAGATGCTTATTGCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGGCCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGAc                                                                                                                                                                                                                      <  1:1398847/250‑1 (MQ=255)

                                  aGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGActg                                                                                                                                                                                                                    <  2:1338398/250‑1 (MQ=255)

                                  aGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGAGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGActg                                                                                                                                                                                                                    >  1:689850/1‑250 (MQ=255)

                                          aTTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGt                                                                                                                                                                                                              >  1:1091427/1‑248 (MQ=255)

                                          aTTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTc                                                                                                                                                                                                             <  2:593242/249‑1 (MQ=255)

                                          aTTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGACTGGt                                                                                                                                                                                                              <  2:629030/248‑1 (MQ=255)

                                               gTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCCtt                                                                                                                                                                                                       >  2:206851/1‑250 (MQ=255)

                                                  ctgcGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTa                                                                                                                                                                                                    <  2:825722/250‑1 (MQ=255)

                                                    gcggtagggTGCTGTGGGTGTGTGCCTGGCTGCCAACACTTTCACGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATg                                                                                                                                                                                                  <  2:1024517/243‑1 (MQ=255)

                                                     ggggACGGGTCTGCCGGTGTGGTCATGGCTGCCAACGCTTTCCCGAAATCGCGTGAAACGCTTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGt                                                                                                                                                                                                 <  2:1072760/247‑1 (MQ=255)

                                                     cGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGt                                                                                                                                                                                                 >  1:1352761/1‑250 (MQ=255)

                                                        gaaggTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGTGCCACTGGTGGAAGAGCTTTATCGCCTCCGCGATCGTCTGCCGGAAAAACTGCGTTATCTGGCGGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACTATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCACTTTGGAAAAAGACGGAAAGGCGACTGGCTGGTCAACATTTTATGTTgc                                                                                                                                                                                              >  2:64299/4‑249 (MQ=255)

                                                        gACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGa                                                                                                                                                                                              >  2:591491/1‑250 (MQ=255)

                                                         aCGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGAt                                                                                                                                                                                             >  2:1449688/1‑250 (MQ=255)

                                                              gctgcCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCa                                                                                                                                                                                         >  1:381704/1‑249 (MQ=255)

                                                               ctgcCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCaaa                                                                                                                                                                                       >  1:1702166/1‑250 (MQ=255)

                                                               ctgcCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTACGCATTTTATGTTGATGGCaa                                                                                                                                                                                        >  2:432809/1‑249 (MQ=255)

                                                                    ggTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGt                                                                                                                                                                                  >  1:1018666/1‑250 (MQ=255)

                                                                      tgtgtTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGTGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTg                                                                                                                                                                                <  2:733369/250‑1 (MQ=255)

                                                                        tgtTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGaa                                                                                                                                                                              <  1:1634105/250‑1 (MQ=255)

                                                                          ttCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAg                                                                                                                                                                             >  1:954954/1‑249 (MQ=255)

                                                                                gCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGaaaaa                                                                                                                                                                       >  2:243900/1‑249 (MQ=255)

                                                                                 cTGCCAACACATTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTGCCGCTACCGCCGGACGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTGCGGAGAAAGACCGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGgaaagaaa                                                                                                                                                                     <  2:1654764/250‑1 (MQ=255)

                                                                                   gacAACACTTTCCCGAAAGCGCGTGAAACGCGTGGGCCACTGGTGGAAGAGCGTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATa                                                                                                                                                                   <  2:1368522/248‑1 (MQ=255)

                                                                                    ccAACACTTTCCCCAAATCGCGTGAGACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTGCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATaa                                                                                                                                                                  <  2:1323814/250‑1 (MQ=255)

                                                                                      aaCACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTCTGTTGATGGCAAATGGGTTGAAGGTAAAAAACAAcc                                                                                                                                                                >  1:554515/1‑250 (MQ=255)

                                                                                      aaCACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAAcc                                                                                                                                                                <  1:1336238/250‑1 (MQ=255)

                                                                                        cacTTTTCCGAAATCGCGTGAAACGCGTGCGCCATTGGCCGAAGAGCTTTATCGTTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGCAGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTCCGCTCTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGTCGGAAAGGCGACTGCCTGGTTAGCATTTTTTGTAGATAGCAAAGAGGTTGAAGGAAAAAAATAATCta                                                                                                                                                              >  2:97242/1‑249 (MQ=255)

                                                                                         acTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt                                                                                                                                                             <  1:389599/250‑1 (MQ=255)

                                                                                         acTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCtt                                                                                                                                                              >  2:1562236/1‑249 (MQ=255)

                                                                                             tCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAAt                                                                                                                                                          <  2:163856/249‑1 (MQ=255)

                                                                                             tCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAAt                                                                                                                                                          <  2:947144/249‑1 (MQ=255)

                                                                                             tCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAAt                                                                                                                                                          <  2:1383880/249‑1 (MQ=255)

                                                                                               ccGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCt                                                                                                                                                       >  2:93875/1‑250 (MQ=255)

                                                                                               ccGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTGCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCt                                                                                                                                                       <  2:1600213/250‑1 (MQ=255)

                                                                                                       gcgTGAAACGCGGGCGGCACTGGTGGAAGAGCTTTATCGCGTCGGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGtt                                                                                                                                               <  1:96360/250‑1 (MQ=255)

                                                                                                        cgTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGttt                                                                                                                                              <  1:192919/250‑1 (MQ=255)

                                                                                                         gTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCACCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGtttt                                                                                                                                             <  2:864724/250‑1 (MQ=255)

                                                                                                          tGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGATGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTTTGTCAGGtttt                                                                                                                                             >  2:1470372/1‑249 (MQ=255)

                                                                                                            aaaCGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAACAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                                                                                                           >  2:124417/1‑249 (MQ=255)

                                                                                                            aaaCGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                                                                                                           >  1:507579/1‑249 (MQ=255)

                                                                                                             aaCGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTtat                                                                                                                                          >  1:527979/1‑249 (MQ=255)

                                                                                                                 cgTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACa                                                                                                                                     <  1:293172/250‑1 (MQ=255)

                                                                                                                     cgcCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAgg                                                                                                                                 >  2:744580/1‑250 (MQ=255)

                                                                                                                     cgcCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAg                                                                                                                                  <  1:1363639/249‑1 (MQ=255)

                                                                                                                      gcCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGTCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAggg                                                                                                                                >  1:993210/1‑250 (MQ=255)

                                                                                                                           tggtggAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGTAAAACTGCGTTATCTGGCCGCTGCGCCACAGCAGGATCCGGAAGGAAATAAGACCATGGGTCGCTTTAGCCGTAAAACCCAACAGC‑CTATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGTATTTTACGTTGATGGCAAAAGGGTTGAAGGAAAAAACTAACCTTTAATTCTGACAGGATTTTATAAACAAAGGGTcgc                                                                                                                            >  2:413425/1‑249 (MQ=255)

                                                                                                                            ggtggAAGAGCTTTGTCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAGGTCTGTCAGGTTTTTATAAACAAAGGGTCGCGa                                                                                                                          <  2:336536/250‑1 (MQ=255)

                                                                                                                             gtggAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGaa                                                                                                                         <  1:834691/250‑1 (MQ=255)

                                                                                                                             gtggAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGa                                                                                                                          <  1:1666385/249‑1 (MQ=255)

                                                                                                                               ggAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAg                                                                                                                       >  2:888730/1‑250 (MQ=255)

                                                                                                                                gAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAg                                                                                                                       <  1:839288/249‑1 (MQ=255)

                                                                                                                                 aaGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGGTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCg                                                                                                                     <  2:481654/250‑1 (MQ=255)

                                                                                                                                   gagCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGc                                                                                                                   >  1:1263383/1‑250 (MQ=255)

                                                                                                                                      cTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCt                                                                                                                >  2:323056/1‑250 (MQ=255)

                                                                                                                                          aTCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCtttt                                                                                                             <  2:1051311/249‑1 (MQ=255)

                                                                                                                                           tCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttttt                                                                                                            <  1:445937/249‑1 (MQ=255)

                                                                                                                                              gtaccGCGAACGGCTGCAGGAAAAACTGCGTTAACAGGCCGATGAGCAACAGCAGGATCCGGAAGGTAATAAGAACATGGTTCGCTTTAGCCGTAAAACCAAACAGCAA‑AAGTATCTACGGAAAAAGACGGAAAGGCGACTGGCTGGTAAGAATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCATTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                                                                                                        <  1:12/247‑1 (MQ=255)

                                                                                                                                              cTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                                                                                                        >  2:1245077/1‑250 (MQ=255)

                                                                                                                                                tCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTg                                                                                                       >  1:85773/1‑249 (MQ=255)

                                                                                                                                                 ccGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTg                                                                                                       <  2:120944/248‑1 (MQ=255)

                                                                                                                                                     gACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCatat                                                                                                  >  1:1600799/1‑249 (MQ=255)

                                                                                                                                                      aCCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                                                                                                >  2:979167/1‑250 (MQ=255)

                                                                                                                                                        cGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATattatt                                                                                              >  1:1648238/1‑250 (MQ=255)

                                                                                                                                                         gTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAAAAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTAttt                                                                                             >  2:456531/1‑250 (MQ=255)

                                                                                                                                                                ggAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttctt                                                                                       >  1:1590403/1‑249 (MQ=255)

                                                                                                                                                                  aaaaaCTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTcac                                                                                    <  2:129783/250‑1 (MQ=255)

                                                                                                                                                                  aaaaaCTGCGTTATCTGGCCGATGCGCCACAGCAGGAGCCGGAAGGTAATAATACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGATCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTGTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTcac                                                                                    <  1:1205473/250‑1 (MQ=255)

                                                                                                                                                                        tGCGTTATCTGGCCGATGCGCCACAGCAGGCTCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTCTTGCATATTATTTTTTCTTCACACCta                                                                               >  2:831988/1‑249 (MQ=255)

                                                                                                                                                                                cTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCATCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTTAGGTTTTTATAAACAAAGGGTCGCGAAAGCGACCCTTTTTTATTGAATATTATTTTTTCTCCACACCTATACATTAAg                                                                      >  1:1579498/1‑250 (MQ=255)

                                                                                                                                                                                   gCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGc                                                                    >  1:870777/1‑249 (MQ=255)

                                                                                                                                                                                          cgcCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATg                                                            <  2:270540/250‑1 (MQ=255)

                                                                                                                                                                                            ccACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAACGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTCTTGCATATTATTTTTTCTTCACACCTATACACTACGGCTATAAATGa                                                           >  2:541872/1‑249 (MQ=255)

                                                                                                                                                                                                agcagGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAg                                                      >  2:218810/1‑250 (MQ=255)

                                                                                                                                                                                                   agGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTgg                                                   >  1:923475/1‑250 (MQ=255)

                                                                                                                                                                                                           gAAGGTAATAAGACCATGGTACGCATTAGACGTAAAACCAAACAGC‑ATATGTCTAGTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGtt                                           <  2:223922/250‑1 (MQ=255)

                                                                                                                                                                                                            aaGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTa                                          <  1:645192/250‑1 (MQ=255)

                                                                                                                                                                                                                      gACCATGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCttttt                                >  2:784160/1‑250 (MQ=255)

                                                                                                                                                                                                                          aTGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTGCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTttatt                            <  2:1499860/250‑1 (MQ=255)

                                                                                                                                                                                                                           tGGTTCGCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTttatta                           <  1:179288/250‑1 (MQ=255)

                                                                                                                                                                                                                                 gCTTTAGCCGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTaaa                      <  1:102417/249‑1 (MQ=255)

                                                                                                                                                                                                                                        ccGTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTAt                >  1:1437249/1‑248 (MQ=255)

                                                                                                                                                                                                                                          gTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGt            >  1:761827/1‑250 (MQ=255)

                                                                                                                                                                                                                                          gTAAAACCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGt            >  1:895075/1‑250 (MQ=255)

                                                                                                                                                                                                                                              aaCCAAACAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGCCGACTGGCTGGTCAGCATTTTAGGTTGATGGCTAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTGTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAAGCGTATTAGTCAcc        <  2:390337/250‑1 (MQ=255)

                                                                                                                                                                                                                                                ccAAACAGC‑ATATGTCTCATCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAGCCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCTTATTAGTCACCCg      <  1:1153050/250‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaCAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAATAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTTTTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGTTATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACTCGc     >  1:173690/1‑248 (MQ=255)

                                                                                                                                                                                                                                                    aCAGC‑ATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAg  <  1:161283/250‑1 (MQ=255)

CGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAG  >  NZ_CP009273/1327568‑1328062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`