Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,245,9970GC33.3% 128.2 / 20.2 63P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
Reads supporting (aligned to +/- strand):  ref base G (11/31);  new base C (21/0);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 6.28e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCT  >  NZ_CP009273/4245748‑4246245
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          
aGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑g                                                                                                                                                                                                                                                            <  1:48939/250‑1 (MQ=255)
   ccttGACGAGCAGGAAACCGGGGGCCCACGGCGGCCAGACGTTGAGGGGCAAAGTGCATCCGCGCAGGGCGCCCCATTTTCTGGGCGAAAGTTTAATCCTTGCCGTGTTCATCCACCATGCGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTGCGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑Ggcg                                                                                                                                                                                                                                                         <  2:563478/247‑1 (MQ=255)
   cccTGCCGAGCAGGCAAGCGGGGGCCTAGGGCAGCCAGACGTGAACGGGCAAAGTGCATACGCGCCGCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTGCGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑Ggcg                                                                                                                                                                                                                                                         <  2:71786/249‑1 (MQ=255)
    ccTGACGAGCAGGCAAACGGGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑Ggcgc                                                                                                                                                                                                                                                        <  2:782291/250‑1 (MQ=255)
             cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CCGCGGCGGGGGTAACTCCCCTTTTT‑TACGCCCC‑Cgccccgcgccca                                                                                                                                                                                                                                                >  2:225024/1‑239 (MQ=255)
                   aaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCAATTCGCCCTTTT‑TTCGCCCC‑CGCGCGCGCCCcaaaaaccc                                                                                                                                                                                                                                         >  2:936084/1‑244 (MQ=255)
                          gCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑TAAGCCCC‑CGCGCGCGACCAAAAATC‑ACCGacacc                                                                                                                                                                                                                                 >  1:959562/1‑249 (MQ=255)
                          gCCTACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACt                                                                                                                                                                                                                                 <  1:452442/250‑1 (MQ=255)
                                  cAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCAt                                                                                                                                                                                                                         <  1:415115/250‑1 (MQ=255)
                                      cAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTGACATCTCCTTTTT‑TCTCCCCC‑CCCGGCCGACCCCACATC‑ACCCACCCCGGCCCCaccctc                                                                                                                                                                                                                     >  2:54351/1‑245 (MQ=255)
                                           gTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCACCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTAACTTCGCCTTTTT‑CACGCCCC‑CGCGCGCGACCCAACATC‑ACCCACACACGCACCACCCGCA‑CAta                                                                                                                                                                                                               >  1:261706/1‑249 (MQ=255)
                                            ttGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATcc                                                                                                                                                                                                              <  1:81146/250‑1 (MQ=255)
                                                 gggCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTTACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGACCAAACATC‑ACCCACACTGGCACCATCTGCA‑CATCCAGAtt                                                                                                                                                                                                         >  1:306508/1‑250 (MQ=255)
                                                    cAAAGTGCATACGCCCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTgg                                                                                                                                                                                                      <  1:861760/250‑1 (MQ=255)
                                                     aaaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACTTCGCCTTTTT‑TCCGCCCC‑CGCGCGCGGCCCAACATC‑ACCGACACTTGCACCATCTGCA‑CATCCAGAATTtgg                                                                                                                                                                                                     >  1:575640/1‑250 (MQ=255)
                                                       aGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGtt                                                                                                                                                                                                   <  1:282011/250‑1 (MQ=255)
                                                         tGCACACGCCCCACCCGCCCCATTTTCGGAGCTATTGTTTTATCCGGGCCGTGTTCTCCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGc                                                                                                                                                                                                 <  2:438168/250‑1 (MQ=255)
                                                           cATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCC‑GGCGCGCGGCCCAACATC‑CCCGACACCGGCACCCTCTGCA‑CATCCAGAATTTGGTTGCTa                                                                                                                                                                                               >  1:808725/1‑250 (MQ=255)
                                                                 cgcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTGCCCATTGCCTTTTT‑CCAGCCCC‑CGCCCCCGACCCACAATCAACCACCACGGCCACCCTCTGAC‑CAACCGGA‑TTGGGGTGCTAccggg                                                                                                                                                                                          >  2:66766/1‑245 (MQ=255)
                                                                      cccgcgcCAGTTTCGGCGCGATAATTTTACCCGCGCCGTGTCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATc                                                                                                                                                                                    <  2:448704/248‑1 (MQ=255)
                                                                       gcgcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCGCAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACCTCGCCCTTTT‑CACGCCCC‑CGCGCGCGGCCAAACAAC‑ACCCCCCCCGGGCCCATCCTCACCACCAAGATTTGGGTTGCTCGGGTGCaaacg                                                                                                                                                                                    >  2:300384/1‑248 (MQ=255)
                                                                         tcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGCTTCTCTTCTCCTTTTT‑TTCCCCCC‑GCCGCGCGCCCACACAAC‑CCCCACACCGGGCCCACCCGCC‑CCTCCCCCATTGGGTTGCTACGCTGCCAACCgag                                                                                                                                                                                 >  2:601199/2‑250 (MQ=255)
                                                                           gcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCAATTCGCCCTTTT‑TACGCCCC‑CGCGCCCGACCCAACATC‑CCCCACACCGGCCCCCTCCTCC‑CATCCCGCATTGGGTTGCTCCGGTGCAAATCgagg                                                                                                                                                                                >  2:291461/1‑248 (MQ=255)
                                                                              aGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACTTTGCCTTTTT‑TACGCCCT‑CGCGCGCGACCAAAAACC‑CCCGACACAGGGCCCATCTTCT‑CACCCCAATTTTGGTTGCTTCCGTGCACATAGAGATAGt                                                                                                                                                                            >  2:696866/1‑250 (MQ=255)
                                                                                tttCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCTCCTTTTT‑TACGCCCC‑CGCGCGCGACCAAACACC‑ACCGACACCGGCCCCCTCTTCC‑CATCCCGATTTTGGTTGCTACGGTGCAAATCGAGGTAGTCg                                                                                                                                                                          >  1:165930/1‑250 (MQ=255)
                                                                                       gCGATATTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACa                                                                                                                                                                   <  1:519418/250‑1 (MQ=255)
                                                                                          aTAGTTTTACCCGTCCCTGGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCt                                                                                                                                                                <  2:398299/250‑1 (MQ=255)
                                                                                           tAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt                                                                                                                                                               <  1:494260/250‑1 (MQ=255)
                                                                                             gTTTTACCCGGCCCGTGTTCACCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAACCTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCCCTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa                                                                                                                                                              <  2:330643/249‑1 (MQ=255)
                                                                                                    ccggcccgtgTTCTCCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc                                                                                                                                                      <  2:41790/245‑1 (MQ=255)
                                                                                                        gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCCCTTCGCCTTTTT‑CACGCCCC‑CGCCCGCGGCCAAACATC‑ACCGACCCCGGCCCCCCCTGCA‑CACCCCGCTTTGGGGTTGTACCGGGCAAAACGGTATAAGCGGGGGACAACCTAATATACacttct                                                                                                                                                  >  2:24514/1‑250 (MQ=255)
                                                                                                          gtgttccccccGCCATAGGGCGCAGCAAAACTGACGGGGAGAACCGCACAAAGCTGCCGCGACCGGGCCACAGTACAGCGAAAAATTGCTGTCCGCCGTTAAGCATGCG‑CAGCGGCGGGTTCCCTCCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACGCTTCttt                                                                                                                                                <  2:162065/241‑1 (MQ=255)
                                                                                                                 aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgt                                                                                                                                          <  1:396050/249‑1 (MQ=255)
                                                                                                                  tCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtgt                                                                                                                                        <  1:13801/250‑1 (MQ=255)
                                                                                                                  tCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGGCCAAAAATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGGTGCTACGGGGCAAATCGAGATAGGCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtg                                                                                                                                         >  1:366772/1‑249 (MQ=255)
                                                                                                                     gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGCCCAAAAAAC‑ACCCACACCTGGACCCTCTCCC‑CAACCCGGGTTGGGGTGGTTCGGGGCGAAAAGAGATAGTAGTGGAACAGGTTAATATACTCTTCtttttgcggggaa                                                                                                                                      >  2:230224/1‑239 (MQ=255)
                                                                                                                           cGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTg                                                                                                                                <  1:510343/249‑1 (MQ=255)
                                                                                                                                  gCGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACAcg                                                                                                                        <  2:250073/250‑1 (MQ=255)
                                                                                                                                        cggACGGGGAGAAACGCACAAAACTTTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGccc                                                                                                                  <  2:445929/248‑1 (MQ=255)
                                                                                                                                           aCGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCGCAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccg                                                                                                                 <  1:300384/248‑1 (MQ=255)
                                                                                                                                            cGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCAACTCTCCTTTTT‑TACGCCCC‑CGCGCGCCACCCAAAAAC‑ACCGACACTGCCACCCCCTGCC‑CAACCACATTTGGGGTTTTACGGTGCACAAAGAGATAGTTGTGGAACAACGTATTAAACTCCTTTTTCTGCGTGTAATAGGGGAACACAcacgcgcccccg                                                                                                              >  2:689108/1‑242 (MQ=255)
                                                                                                                                             ggCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTGCCTTTGCCTTTTT‑TACCCCCC‑CGCGCGCGACCCAACACC‑AACCACCCTGGCACCACCTGCC‑CATCCCGATTTGGGGTGGTACCGGGCTAATCCAAATAGTCGGGGACAAGCTTTATAAAACATTCTTTTTGCGCGTACTAGGTGGCCACACCAGGGccgccgc                                                                                                             >  2:862855/1‑249 (MQ=255)
                                                                                                                                                           aaaaCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGACCAAACATC‑ACCGACACTGGCACCCTCTGCA‑CATACAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGGACAGCTTAATAAACTCTTCTTTCTGCGGGTAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAg                                                                                               >  1:234038/1‑250 (MQ=255)
                                                                                                                                                            aaaCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGACCCAACATC‑ACCGACACTGGCACCATCTGCC‑CATCCAGATTTTGGGTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAAAACATAgc                                                                                              >  1:256325/1‑250 (MQ=255)
                                                                                                                                                               cggTCGCGACCGCCCAACAGTACAGCAAAAAATTTCTGTACGCGGTAAAGCATCCG‑CGGCGGCGTGTCCACTTCGCCTTTTC‑CACGCCCG‑GCCGCGCGACAAAACACC‑ACCGACACGGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTAGGGTGAAAAGCGAGATATTGGGGGAACAGCTTAATAGCCTCTTCTTTCGGGGTGTAATAGGTGACCACACGCGGCCCGCCGTGAATGAACACATAGcgcg                                                                                           <  2:814490/248‑1 (MQ=255)
                                                                                                                                                                  tCGCGCCCGAGCCACAGTACAGCAAAAAATTTCTTTACGCCTTAAAGCATCCG‑CAGCGGCGGTTTCCCTCCGCTTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑CCCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCgg                                                                                          <  1:458857/248‑1 (MQ=255)
                                                                                                                                                                  ccgcgCCCGAGCCACAGTACAGCAAAAAATTTCTGTCCGCCGTAAAGCATACG‑CGGCGGC‑GGTTCCCCTCCCCTTTTTCCCCGCCCC‑GGCGCGCGACAAAACATC‑CCCGACACTGGCACCATCTGCA‑CACCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCgg                                                                                          <  1:397429/247‑1 (MQ=255)
                                                                                                                                                                      gccggcgCCACAGTACAGCAAATATTTTCTGCCCGCCGTGAAGCAACCG‑CAGCGGGGTTTTCAC‑TCCCCTTTTT‑CCCCCCCCGGGCGCGCGACAAAACCCC‑CCCGACACGGGCACCTTCTGCA‑CATCCAGTTTGGGGTTGCTACGGTGAAAAGCGGGATAGTCGGGAAACAGTTTAATAGACTCTTCTTTCGGGGTGAAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAg                                                                                    <  2:566085/244‑1 (MQ=255)
                                                                                                                                                                                          aaaaaTTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑CGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGGTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAAACTCTTCTTTCGGCGTGTAATAGGTGAACACAAGCGGCCCCCCGTGAAAGAACAACAAACGCGGCAGGAGCGGGCCGTCGATTTTCcgcg                                                                >  1:228648/1‑250 (MQ=255)
                                                                                                                                                                                          aaaaaTTTCTGCCCCCCTTTAACCA‑ACGCCGGGGGCGGTTTCAC‑TCCCCTTTTT‑CCCCCCCCGGGCGCGCGACAAACCACC‑CCAGACACTGGCCCCATCTGCA‑CATCCAGATTTGGTTTGCTCCGGTGAAAATCGAGATAGTCGGGAAACAGCTTAATAGACTCTTCTTCCGGCGTGTAAGAGTTGACCACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCg                                                                <  2:903078/250‑1 (MQ=255)
                                                                                                                                                                                              aTTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCCCCGGGAATGAACACATAGCGCGGCAGTAGCGGGCCGTCGATTTCCAGCGGctc                                                            >  1:290441/1‑250 (MQ=255)
                                                                                                                                                                                                                   tccG‑CAGCGGCGGGTTCCCTCCGCCTTTTT‑CCCCCCCC‑GGCGCGCGACCAAACATC‑CCCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAtg                                       <  1:936084/248‑1 (MQ=255)
                                                                                                                                                                                                                     cG‑CAGCGGGGGTTTCACTTCCCTTTTTC‑CACGCCCC‑GGCGCGCGACAAAACATC‑CCCGACACGGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAtgtg                                     <  1:317256/250‑1 (MQ=255)
                                                                                                                                                                                                                         aggggcggTTTCCCTCCGCCTTTTT‑CCCCCCCG‑GGCGCGCGACAAAACACC‑CCCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGAAAATCGAGATAGTCGTGAAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTAAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCa                                  <  1:773540/247‑1 (MQ=255)
                                                                                                                                                                                                                             gcggGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGaca                              >  1:363310/1‑250 (MQ=255)
                                                                                                                                                                                                                                 gTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTgg                          >  1:848401/1‑250 (MQ=255)
                                                                                                                                                                                                                                   tCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgc                        >  1:44470/1‑250 (MQ=255)
                                                                                                                                                                                                                                    cccTTCCCCTTTTC‑CCCGCCCC‑GGCGCGCACCAAAACCAC‑CCCGCCGCGGGCACCTTCTGCT‑CAGCCAGATTTGGGTTGCGAGGGTGCAAACGGAGATAGTCGGGGAACAGCTTAATAGACTCTTCTTTCGGGGTGTAAGGGGTGACCACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcg                       <  2:614530/248‑1 (MQ=255)
                                                                                                                                                                                                                                          gCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc                 >  2:484717/1‑250 (MQ=255)
                                                                                                                                                                                                                                            cTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGt               >  1:606052/1‑250 (MQ=255)
                                                                                                                                                                                                                                                tt‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAg            >  1:750590/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 t‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAtg                                       >  1:960076/1‑221 (MQ=255)
                                                                                                                                                                                                                                                        ccc‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTg    >  1:395/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          c‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCt  >  1:82971/1‑250 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          
AGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACG‑CAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACTGGCACCATCTGCA‑CATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCT  >  NZ_CP009273/4245748‑4246245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑