| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,331,852 | 0 | A | C | 33.3% | 175.9 / 11.3 | 85 | D42A (GAC→GCC) | melA | alpha‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base A (44/12); new base C (1/27); total (45/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGTATTATGCGCGCGCTACG > NZ_CP009273/4331606‑4332101 | cTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGAccc > 2:312473/1‑250 (MQ=255) aGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGAcccc < 1:86785/248‑1 (MQ=255) ttACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGc > 1:938274/1‑250 (MQ=255) ctgctgCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGTCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTg > 1:330888/1‑250 (MQ=255) tgctgcTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTg > 2:940824/1‑249 (MQ=255) ggATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATAtt > 1:540841/1‑250 (MQ=255) aGTTTATGGAAATGCTCAACCTGGAAGCCGGGGTTTTTCTGAAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCCCCAAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAACC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGc < 1:11235/250‑1 (MQ=255) gTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCATGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCg > 1:962625/1‑250 (MQ=255) tttATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGt < 1:235156/250‑1 (MQ=255) cTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGAt > 2:78376/1‑250 (MQ=255) cTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGAt > 2:864964/1‑250 (MQ=255) tCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGAtt > 2:791616/1‑250 (MQ=255) cAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGAtt > 1:195474/1‑249 (MQ=255) aaCCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGAtt > 1:695882/1‑248 (MQ=255) gAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTcagcag < 1:47192/248‑1 (MQ=255) aGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGc > 2:67432/1‑250 (MQ=255) ccGGAGGTTTTCTGC‑GATTCCGCCTGCCATGATGTAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCa < 2:315626/250‑1 (MQ=255) cGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAg > 2:847290/1‑250 (MQ=255) gAGGTTTTCTGCAGATT‑CGCCCGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGCAAGCTGATGGATTCAGCAGGGGCCAGCg > 1:371124/1‑250 (MQ=255) cGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCcac > 2:794298/1‑250 (MQ=255) gCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCcaca > 1:132600/1‑250 (MQ=255) ccATGATGAAGTTATTCAAGCAAGCTAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAACATCACCTGCCACCCCCaa > 2:423047/1‑250 (MQ=255) gaATTTATTAAAGAAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCTTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTAAAACC‑GGCGAATATTGCCCTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaaag < 1:874220/250‑1 (MQ=255) ttATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TGGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGc < 1:478461/250‑1 (MQ=255) ttCAACCAAGCCTGAAGTTCTGCTGGAGTTCTGCACCAAAATTTATATTTATCGCGGGTGGT‑TCG‑AGTTTTTTCTTTAAAAATT‑TT‑TTGGGTGA‑TGTGTCCAATCGCGGGGCGCGAAAAAC‑GGAGCATATTGCCTTGATGG‑ACT‑GTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCtt < 2:522458/250‑1 (MQ=255) caagcCAGGAGATCTGCATGATTTCTGCACCCAAAATTACATTTATCGGCGCGGGT‑GCG‑ATGATTTTCTTTAAAATTA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGa < 1:85934/250‑1 (MQ=255) caagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGa > 1:142811/1‑250 (MQ=255) aagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCACCAGAAAGAAGCCTTAGAGGAt > 2:879667/1‑250 (MQ=255) aagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAggca > 2:459159/1‑248 (MQ=255) aagcCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGAt > 2:86656/1‑250 (MQ=255) aagcAAGGAGATCAGCTGGAGGCTTGCACCAAAATTTAAATTTTTCGGCGTTGGT‑TCG‑ACGATTTTCGTAAAAAATA‑TT‑CTTGGTGA‑GGTTTTCCACCGCGGGGCGCTGAAAAC‑GGCGCATTTTGCCTTGAGGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGAt < 2:907272/250‑1 (MQ=255) ccAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGcc > 1:74848/1‑250 (MQ=255) ccAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGcc > 1:163641/1‑250 (MQ=255) cAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCACCAGAAAGAAGCCTTAGAGGATGcg > 2:812729/1‑248 (MQ=255) aGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGAATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGa > 2:448443/1‑250 (MQ=255) agaTCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAttt > 1:724562/1‑250 (MQ=255) agaTCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGAtgc > 2:360134/1‑248 (MQ=255) ttgtttccACCAAAAATTACATTTATCGGCGTTGTT‑TCG‑AGTATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTCCCACCGCGGGGCGCTGAAACC‑GGCGAATTTGGCCTTGAGGG‑ACT‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGtggtgg < 2:646130/243‑1 (MQ=255) gtgTCTCCACACAAATTTAAATTTCGGGGCG‑TTGT‑TCGAAAGATTTTC‑TTAAAAATA‑TTGCTTGGGAG‑TGTGTCCCCCGGCGGGCCTCTAAAAAC‑GGCGCATATTCCCCTGAGGG‑ACA‑TTGCCCCA‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATGCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCgtggtg < 2:412623/248‑1 (MQ=255) cggccccaaaaaTCACATTT‑TCGGGGGCGGTGTCG‑AGGTTTTTCGTAAAAAAT‑‑TT‑TTTGGGGAGTGTGTCCCACCGGGGGGCGCGAAAAAC‑GGCGAATATGCCCCTGATGA‑AAA‑TGGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCAt < 2:948869/241‑1 (MQ=255) cTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCAtt > 1:564692/1‑250 (MQ=255) tGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCAttt > 1:867575/1‑250 (MQ=255) ccccaaaaaTTACTTTTATGGGCGTTGTT‑TCG‑ACTTTTTTGTTAAAAAATT‑TT‑TTTGTTAT‑TGTGTCCCTCCGGGGGCCGC‑GAAAACGGGCGCAT‑TTTCCCTTGTGG‑ACATTTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCGGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTc < 2:552455/244‑1 (MQ=255) cACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTTGCAttt > 2:208537/1‑248 (MQ=255) tttcATTTATCGGCTCTGGT‑TCG‑ACTATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTTTCCCAGCGGGGGGCGCAGAAAAC‑GGCGCATTTTCCCCTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCgg < 2:358256/247‑1 (MQ=255) aCATTTATCGGCGCTGTT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑GGTTTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TGGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGt < 1:597167/249‑1 (MQ=255) tCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGCTTTTGTCGTGGTGGCATTTCAGATTGGCGGGTGTGaaac > 2:766802/1‑248 (MQ=255) tCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGCTGTGaat > 2:317417/1‑248 (MQ=255) cGGCTCGGGT‑TCG‑ACGTTTTTCTTAAAAAATT‑TT‑CTGGGTGA‑TGTTTTCCACCGGGGGGCCCTGAAAGG‑GGCGCTTTTTCCCTTGAGGA‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCt < 2:875173/250‑1 (MQ=255) cGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGGTATTaaaat > 2:29628/1‑247 (MQ=255) gcTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTTGACCTTgga > 1:144634/1‑248 (MQ=255) tGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGTCGGCTATTAACCttttaag > 1:960227/1‑245 (MQ=255) tt‑tCG‑ACGTTTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCACCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACg < 1:204604/247‑1 (MQ=255) tt‑tCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGt < 1:51786/249‑1 (MQ=255) aTTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTTAAACTTGCACGGGGACttgtt > 2:807770/1‑246 (MQ=255) ttaaaaaaTT‑TT‑CTTGGTGG‑GTTTGCCCACCGCGCGCCGCTAAAACC‑GGCGCATTTTCCCCGGATGG‑ACT‑TGGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATGCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAgg < 2:330888/247‑1 (MQ=255) cgtAAAAATATTT‑TTTGGGTA‑TGTTTCCAATCGCGGGGCGC‑GAAAACGGGGGTATTTTCCCCTGAGGG‑AAA‑TTGACCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGa < 2:540841/247‑1 (MQ=255) tAAAAATA‑TT‑CTTGGTGA‑TGTGTCCCACCGCGGGGCGCGGAAACC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGt < 1:567738/249‑1 (MQ=255) aaaaaaTT‑TT‑TTTGGGGA‑GTGGTCCCACGGCGAGGCGCTAAAAAC‑GGCGCATATTGCCCTGATGG‑CCT‑TGGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGt < 2:243217/248‑1 (MQ=255) aaTA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTGCACCGTGACTTATTTTGGTGTCTGt > 1:628860/1‑249 (MQ=255) ata‑tT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGGGACTGATTTTGGGGGCTgga > 1:321298/1‑247 (MQ=255) ta‑tT‑TTGGGTGA‑TGTGTCCCACCGGGAGGCGCTGAAAGC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTaa < 2:321298/249‑1 (MQ=255) cTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGGGGCTGATTTTGAGGGCTGTAAGCgg > 1:263862/1‑249 (MQ=255) ttGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTGCACGGGGACTGATTTCGTGGTCTGTAAGCGGCa > 1:867698/1‑250 (MQ=255) ggTGA‑TGTGTCCCGTCGCGGGGCGCGAAAACC‑GGCGCATATTGCCCGGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGc < 1:643789/247‑1 (MQ=255) a‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTGCACGGTGGCTGATTTTGGGGGCTGTTAGCGGCATGGTCt > 1:257537/1‑250 (MQ=255) ttCCAGCGGGAGGCGCTCAAACC‑GGCGCATATTGCCTGAATGG‑ACT‑TTGACCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAAc < 1:240127/250‑1 (MQ=255) cATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGGCTGATTTTGAGGTCTGTAAACGGCATGGTCTGGAACaa > 1:342860/1‑249 (MQ=255) cccgAGGCGCGAAAACC‑GTGGAATATTGCCCGAATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCa < 1:544344/248‑1 (MQ=255) gcgAGGCGCTGAAAAC‑GGCTCATATTGCCCGGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTATAAGAGTCGCATATTGTTGTGCTTTAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAATAAGCCTTATAGGATGCCGATTTTGTCGTGGTGGCATTTCAGCTTTTCTTTTATTAACCTTGCACTTTTACTCATTTCGTTCTCTTTAAGCGGCATGTTCTGGAACAAACCAt < 1:154030/250‑1 (MQ=255) gagcctgtGAAACC‑GGGGAATATGCCCTTGAGGG‑ACA‑‑TGACCCCAA‑CCCGGCTGGAAGATCGGCATATTGTGGTGCGTAAGCGGATGGATTCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑Tg < 2:17738/243‑1 (MQ=255) gggCGTTGAAACC‑GGCGCTTATTGCCCTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGc < 1:955920/249‑1 (MQ=255) ctaaaaCGGGCGAATTTTCCCCTGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATa < 2:529261/248‑1 (MQ=255) aaaaC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAc < 1:512412/248‑1 (MQ=255) aaC‑GCCGCATATTCCCTGGAGGG‑ACA‑TGACCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTg < 2:257537/250‑1 (MQ=255) c‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACGTGCACGGGGACGGATTTGGAGGTGTGTAAGAGGCAGGGGGTGGAAAAAACCAT‑TGCCGATAC‑Gtgtgg > 1:767492/1‑246 (MQ=255) ggcaatttGCCCTGAAGGAACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCCCCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGc < 1:432371/244‑1 (MQ=255) catttgtccttGAGGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCgg < 1:364752/240‑1 (MQ=255) cATATTGCCCTGTGGG‑ACA‑TTGCCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCgg < 2:132600/250‑1 (MQ=255) atatTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAATTTTCTCATATTGTGGTTCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACagaatta > 1:320801/1‑115 (MQ=255) cTGATGG‑ACA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGtatta < 1:360062/250‑1 (MQ=255) cccgaagggctttGACCCC‑ACCCCGCCTAGAAGATTCGAATATTGGGGGGCATAAGCTGATGGATTCCGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCTATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGtat < 1:564635/239‑1 (MQ=255) aaCA‑TTGGCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGTATTATgcgc < 1:249358/248‑1 (MQ=255) cA‑TTGCCCCC‑C‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGTATTATgcgcgcg < 1:21496/250‑1 (MQ=255) gACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAAACTTGCCAGGGGACTGGTTTTGAGGTCTGTAAGCGGCATGGTCTGGGACAAACCAA‑TTCCGGTTCAGTTGGGGCC‑GGCGGGATTTTTCGCGCGCTAc > 1:856783/1‑250 (MQ=255) aCCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTACCCTTCCACGGCGCAAGATTTTCGGGGCTGTTAGTGGACTGGTCTGGAAAAAACCCTACGCGGATA‑‑GATGGGGCAGGGCGGGGTTATGCGCGGCCTACg > 2:620355/1‑250 (MQ=255) | CTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATT‑CGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGT‑TCG‑ACGATTTTCGTTAAAAATA‑TT‑CTTGGTGA‑TGTGTTCCATCGCGAGGCGCTGAAAAC‑GGCGCATATTGCCCTGATGG‑ACA‑TTGACCCC‑A‑CCCGCCTGGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAT‑TGCCGATAC‑GTTGGGGCCGGGCGGTATTATGCGCGCGCTACG > NZ_CP009273/4331606‑4332101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |