Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,718	0	G	C	32.7%	77.5 / 13.4	53	pseudogene (520/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base G (13/22);  new base C (12/5);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 3.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATAATTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAG  >  NZ_CP009273/2221469‑2221967                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                          ataatTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTccg‑ccg                                                                                                                                                                                                                                                                           <  2:470313/250‑1 (MQ=255) ataatTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTGCT‑CGCGTTccg‑ccg                                                                                                                                                                                                                                                                           >  1:506217/1‑250 (MQ=255)         cattttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑c                                                                                                                                                                                                                                                                  <  2:151282/249‑1 (MQ=255)          attttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTGC‑‑CGCGCTGCG‑CCA‑CCCCTG‑cct                                                                                                                                                                                                                                                                >  1:623048/1‑248 (MQ=255)           ttttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑ttt                                                                                                                                                                                                                                                              <  2:215069/250‑1 (MQ=255)                 ccGCGACATCGTTGAGCTTCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGt                                                                                                                                                                                                                                                        <  2:147134/250‑1 (MQ=255)                     gACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTAccc                                                                                                                                                                                                                                                    <  2:345382/250‑1 (MQ=255)                     gACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTAccc                                                                                                                                                                                                                                                    <  1:474826/250‑1 (MQ=255)                        aTCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCCATTGGCGGGGTTTGTC‑CCCGTTCCC‑CCC‑CCCCGG‑T‑TTTAACCGACCCCA‑g                                                                                                                                                                                                                                                 >  2:403963/1‑250 (MQ=255)                          cGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTGTC‑CCGGCTCCC‑CCC‑CCCGGG‑C‑TTTAACCgatcccgggg                                                                                                                                                                                                                                                >  2:39735/1‑241 (MQ=255)                            ttGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCggg                                                                                                                                                                                                                                            <  2:83859/250‑1 (MQ=255)                               aGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTGCT‑CCCGGTCCG‑CCG‑CCCCTG‑C‑TTTAACCCGACCCA‑gggggg                                                                                                                                                                                                                                            >  1:975501/1‑247 (MQ=255)                                       ccGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCt                                                                                                                                                                                                                                <  1:31883/248‑1 (MQ=255)                                       ccGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTcc                                                                                                                                                                                                                              <  1:854832/250‑1 (MQ=255)                                           gTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTGCT‑CGCGTTACG‑CCC‑GCCGTG‑C‑CTTAACCCCACCCA‑GGCGGGCCAG‑T‑A‑CCCCCGccc                                                                                                                                                                                                                          >  1:344515/1‑250 (MQ=255)                                                 cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTc                                                                                                                                                                                                                     <  2:1030760/249‑1 (MQ=255)                                                 cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCt                                                                                                                                                                                                                    <  2:821602/250‑1 (MQ=255)                                                       ttAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTGCT‑CGCGGTCCG‑CCG‑CCCCTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCCATTCTTT‑tccc                                                                                                                                                                                                             >  1:627301/1‑250 (MQ=255)                                                           tatCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTCT‑CGCGGTCCG‑CCG‑CCCCTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑GAA‑CCCCCCCCCGCTTCTTT‑TCCCCG‑a                                                                                                                                                                                                         >  1:123638/1‑249 (MQ=255)                                                               ggTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATc                                                                                                                                                                                                     <  2:433030/249‑1 (MQ=255)                                                                   tCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCga‑g‑a                                                                                                                                                                                               <  1:976799/249‑1 (MQ=255)                                                                      acacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCCGCAACTGGC‑GGGTTTTCT‑TGCGTTCCG‑TCC‑CCCCTGCC‑TTTTACCG‑ACCCACGCCGGC‑CGG‑TAA‑CC‑CCCCCCCCTTATTT‑TCCCAC‑CTATCGA‑G‑GT‑ggc                                                                                                                                                                                          >  2:390066/1‑248 (MQ=255)                                                                         ccTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑Gc                                                                                                                                                                                      <  2:696854/250‑1 (MQ=255)                                                                                   aaaaattacccccTTTCTGATAAAGCGGGCGATCGGCTGCAATAATGGTTTCCTGTTTTTTTCTTTTTTGCGACAGGA‑CGTTTTTTACCGCCCCCTGGTTTTCCCCCCCCCAGTACAGGCGGGCTCCGCTCCCAGCCAGAATTGGGC‑GGGTTTGCC‑CGCTTCCCG‑CCC‑CCCGTG‑T‑TTAACCCGTCCCCG‑GGCGGG‑CAG‑TAC‑CCCCCGCCCGATTCTTC‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTaa                                                                                                                                                                            <  2:829433/240‑1 (MQ=255)                                                                                    agaagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTGCT‑CGCGTTCCG‑CCG‑CCCCGG‑C‑TTTTACCGTACCCA‑GGCGGG‑CAG‑GAA‑ACTCCCCCCGATTCTTT‑TCCCCG‑ACATCGA‑G‑AT‑GCCA‑CCCCGCCCGTAAg                                                                                                                                                                           >  1:892075/1‑250 (MQ=255)                                                                                            tCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTTAACGTACCCC‑GGCGGG‑CAG‑CAA‑CCTCCGCCCGGTTCTTT‑TCCCCG‑AGATCGA‑G‑AT‑GCCA‑ACCCCCCGTTAAGC‑CCAAAg                                                                                                                                                                   >  1:755454/1‑249 (MQ=255)                                                                                                gTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTCT‑CCCGTTCCG‑CCGCCCCCGG‑T‑TTTAACCG‑ACCCC‑GGCGGG‑CGG‑TAACCCCCCCCCCGCTTCTTTCCCCCAG‑ATCTAG‑‑G‑AG‑GCCC‑CCCCCCCGTTAA‑C‑CCAAAGTAccc                                                                                                                                                              >  2:931459/1‑249 (MQ=255)                                                                                                       tataGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTGT‑CGCGTTTCGCCCC‑CCCGGG‑T‑TTAAACCGTCCCCA‑‑GCGGG‑GCG‑GAA‑ACTCCCCCCGGTTCCTT‑TCCCCG‑ATTTTCA‑G‑AG‑GTCC‑ACCCGCCGCTAAACGCAACAGCACCTTTggg                                                                                                                                                         >  2:583708/1‑248 (MQ=255)                                                                                                        ataGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTCT‑CGCGTTCCC‑CCC‑CCCGTG‑C‑TTTAACCGTACCCC‑GGCGGG‑GAG‑GAA‑CCTCCGCCCGATTCTTT‑TCCCCG‑ATATCGA‑G‑AT‑GGCA‑ACCCGCCCGTTAGC‑CCAAAATACCCCTTGTGTa                                                                                                                                                      >  1:564141/1‑250 (MQ=255)                                                                                                            ctcgcgagcgcgcgcgATTATGGTTTTCTGCTTTTTTTCGTTTTGCGGAAAGA‑CCGTTTTTAGCGCCGCCTGGTTTTGCCCCTCCCAGTACAGGGGGCCTCCGCTGCCAGCCAGCATTGGGG‑GGGTTTGCT‑CGCGTCCCG‑CCG‑CCCGTT‑T‑TTTACCCGTCCCCG‑GGGGGG‑CAG‑TAC‑CCCCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑CCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCAc                                                                                                                                                  <  2:201148/238‑1 (MQ=255)                                                                                                                    cGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCGGCCAGCAATTGGC‑GGGGTTTCT‑CGCGTTCCC‑CCC‑CCCCCG‑CTTTTAACCTAACCCA‑‑GCGGG‑GGG‑TAA‑CCTCCCCCCCGTTCTTT‑TCCCCG‑ATTTTGC‑G‑ATGGCCA‑CCCCCCGCTTCACC‑AAAAAGACCCGTGGGGGTACCCCggccggg                                                                                                                                           >  2:758700/1‑245 (MQ=255)                                                                                                                                 ggTGTTTTTCTTTTTTGCTTTTGGCAGCAGGA‑CGTTTTTTACCGCGGCCGGGGTCGCCCACCCCCAGTACAGGCGGGCCACGCTCCCAGACAGCAATGGGC‑GGGTGTTCT‑CGCTTCCCG‑CCG‑CCCTTG‑T‑TTAAACCGTCCCGA‑GGCGGG‑CAG‑TCC‑CCTCCGCCGGATTCTTC‑CCCCAG‑ATAACGA‑G‑AT‑GCCG‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGcc                                                                                                                             <  2:590476/250‑1 (MQ=255)                                                                                                                                    gtTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTTCT‑CGCGTTTCC‑CCC‑CCCCTG‑C‑TTTTAACCTAACCC‑CGCGGG‑GCG‑GAA‑CGTCCCCCCCCTTCTTT‑CCCCCG‑AGAACGA‑G‑AT‑GGCA‑GCCCGCCCCTTAGC‑CAAAAAGACCCGTTGTGTTCCACGGGGCGGGCCGACCTGCGGCCg                                                                                                                            >  2:118112/1‑248 (MQ=255)                                                                                                                                       cTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTCT‑CCCGTTCCC‑CCC‑CCCCTG‑C‑TTTTACCGTACCCC‑GGCGGG‑GCG‑TAA‑ACCCCCCCCCCTTCTTT‑TCCCCC‑ATATCGG‑GAAG‑GCCG‑CCCCCCCGTTAGCC‑CAAAATAACCGTGGGGGACCCCGGGGCCGGCCGACCCTCGGGCCgggcg                                                                                                                        >  2:29402/1‑247 (MQ=255)                                                                                                                                        ttctttttttCTATTTGCGGAAACA‑CGGTTTTAACCGCCCCGGGGGTTGCCCCCTCCCAGTACAGGGGGTCCCCCCGCCCAGCCAGAAATGGGG‑GGTTTTGCT‑CGCTTCCCG‑CCG‑CCCGTG‑C‑TTTAACCGTCCCCA‑GGCGGG‑CAG‑TAC‑CCCCCGCCCGATTTTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑g                                                                                                                      <  2:506217/244‑1 (MQ=255)                                                                                                                                         tctttttttGTATTTGGTG‑AAGACCCGTGTTTACCGCCGCGTGGGTCTACCACACCCAGCACAGGGGGTCCGCGCTCCCACCCAGAATTGGGC‑GGGTTCGCC‑CTGCTCCCG‑CCG‑CCCGTG‑T‑TTTAACCGTA‑CCA‑GGGGGG‑AGGTTAA‑CCTCCGCCCTTTTCTTT‑CCCCAG‑AAATCGG‑G‑AG‑CCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑Gtt                                                                                                                    <  2:975819/246‑1 (MQ=255)                                                                                                                                                ttCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGGTGCT‑CGCGTTCCG‑CCC‑CCCGTG‑C‑CTTTAACGTACCCA‑AGCGGG‑CAG‑GTA‑CCTCCCCCCCACTTTTT‑TCCCCG‑ATATACA‑G‑CA‑GCCA‑GGCCGGCCTTCAGC‑CAAAAAGACCCGTGGGTTGCCACGGGCCGGGCGGACCTGGGGCCGGGTC‑GCTCagcgg                                                                                                              >  2:634827/1‑246 (MQ=255)                                                                                                                                                    aTTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTTGC‑GGGGTTTCC‑CGCCGTCCG‑CCC‑CCCGCG‑G‑TTTAACCCTTCCCA‑GGGGGG‑GAG‑CTA‑CCCCCGCCCGCTTTCTT‑CCCCCG‑ATACCGAGG‑GT‑GCAA‑GCCCCCGGTAAGGC‑CCAAACACCGGTTGGGGACCCCCGGGCCGG‑CGTGCCTGCCGCCGGCGC‑GTTTATA‑ACaaaac                                                                                                        >  2:62254/1‑248 (MQ=255)                                                                                                                                                       tgctgcAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGTTTGT‑CGCGTTTCC‑CCC‑CCCCTG‑C‑TTTTACCCGACCCC‑AGCGGG‑CAG‑TAA‑ACCCCCCCCCCTTCTTT‑TCCCCG‑ATATCGA‑G‑AA‑GCCA‑ACCCCCCCTTTAAC‑CAAAAGCACCCTTTGGGTACCCCCGGGCGGGCGGCACTTCCGCCCGAGC‑GTTTAGAGGCAAACgcc                                                                                                      >  2:845589/1‑248 (MQ=255)                                                                                                                                                                            cgccgcCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGGGTGGC‑CGCGGTTCC‑CCG‑CCCCTG‑G‑TTTTACCCGACCCA‑GGCGGG‑GAA‑TAA‑ACCCCCCCCCATTCTTT‑TCCCCG‑AAATCGA‑G‑GT‑GCCACCCCCCCCGTTAGGC‑AAAAGGAACCTCTGG‑GTATCCCGGGGCGGGCGCGACTTCCGGCGGAGCTGTTC‑GA‑GCACACGGCCCCCGTAAGCCTTCTCCCgtc                                                                                 >  2:247032/1‑248 (MQ=255)                                                                                                                                                                                   gtgtttcgCCTCTCCCAGCCCAGGCGCTCTACGCTCCCAGCCAGCATTTGTG‑GGGTTTCCC‑CGCTTCCCG‑CGC‑CCCGTT‑C‑‑TTACCCCTTCCCAGGGGGGG‑CAT‑TAA‑CCCCCGCCCCATTTTTC‑CCCCAG‑ATATCGA‑A‑AT‑GCCG‑CCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACAtttttt                                                                          <  2:1004617/243‑1 (MQ=255)                                                                                                                                                                                             aCTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTTCT‑CGCCGTTCG‑CCG‑CCCCTG‑C‑CTTTAACGTATCCC‑GGCGGG‑GAG‑GAA‑ACTCCGCCCGCTTCTTT‑TCCCAC‑ATATCTG‑G‑AT‑GCCA‑GCCCGCCGCTTAGC‑CAAAAATAACGGTGGGGGACCCCCGGGCGGGCGTACCTGCGGCCGGGTC‑GTTCAGG‑GCACACGGCCCCAATTAGCCTTCTGGCGACATTTTTTGCCGGTccct                                                                >  2:236517/1‑248 (MQ=255)                                                                                                                                                                                                cccAGTCCAGGCGGCCCCCGCTCCCACCCGGC‑ATTGGG‑GGGTTTGCTCCGCCTCCCC‑CCC‑CCCGTC‑T‑TTTACCCGACCCCG‑GGGGGG‑AAG‑TAC‑CCCCCGCCCGATTCTTC‑CCCCAA‑ATACCGA‑G‑AT‑CCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATc                                                             <  2:396835/250‑1 (MQ=255)                                                                                                                                                                                                        cggcggacaccGCTCCCCGCCAGCAAGTGGG‑GGGTTGCCT‑CGCGTCCCG‑CCC‑CCCTTT‑C‑TTCACCCGTCCCCG‑GGCGGG‑CAG‑TAC‑CCCCCCCCCGATTCTTC‑CCCCAA‑ATATCGA‑G‑CT‑CCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGaaa                                                     <  2:5223/240‑1 (MQ=255)                                                                                                                                                                                                          gCGGGCTACGCTCCCACCCAGCAATTGGC‑GGGTTTGCT‑CGCGTCCCC‑CCG‑CCCGTG‑C‑TTTAACCGTCCCCG‑GGCGGG‑CAG‑TAC‑CCTCCGCCCGATTCTTC‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAg                                                   <  1:336597/250‑1 (MQ=255)                                                                                                                                                                                                               cTACCCTGCCAGCCAGCAATGGGG‑GGGTTTGCT‑CGCGTCCCG‑CCG‑CCCGTG‑C‑TTTACCCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTC‑CCCAAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGAt                                              <  1:403963/250‑1 (MQ=255)                                                                                                                                                                                                                tACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGGAAAGTCGATc                                             >  1:363499/1‑250 (MQ=255)                                                                                                                                                                                                                        cagccagcAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGAAC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCCCCATATCCGCCGAAAAGACGATCCGCGGAc                                      >  1:996546/1‑249 (MQ=255)                                                                                                                                                                                                                          ggcagcAAGTGGGGGGGTTGGCC‑CGCGTCCCC‑CCC‑CCCGTC‑C‑TTTAACCGTCCCCG‑GGCGGG‑CAG‑TAC‑CCCCCGCCCGTTTCTTC‑CCCAAG‑ATACCGG‑G‑AT‑CCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCa                                    <  2:442055/248‑1 (MQ=255)                                                                                                                                                                                                                                    ggC‑GTGTTTGCT‑CCCGTCCCG‑CCG‑CCCGTG‑T‑TTTAACCGTCCCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCTGATTCTTC‑CCACAG‑ATATCGA‑G‑GT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTgg                          <  1:689203/249‑1 (MQ=255)                                                                                                                                                                                                                                              gCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCtt                >  1:930531/1‑250 (MQ=255)                                                                                                                                                                                                                                                  cgcgTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGc             >  1:352229/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gcgTTCCG‑CCG‑CCCGTG‑C‑TTTACCCGTCCCGG‑GGCGGG‑GAG‑TAC‑CCCCCGCCCGTTTC‑TT‑CCCCAGAAGAAGGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGcc            <  1:403467/250‑1 (MQ=255)                                                                                                                                                                                                                                                   gcgTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGcc            >  1:72017/1‑250 (MQ=255)                                                                                                                                                                                                                                                      ttCCG‑CGG‑CCGGTG‑C‑TTTAACCGTCCCCA‑GGGGGG‑AAG‑TAC‑CCCCCGCCCGTTTCTTT‑CCCCAG‑ATTTCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTgg         <  1:587618/250‑1 (MQ=255)                                                                                                                                                                                                                                                            ccg‑ccCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt     >  1:953809/1‑249 (MQ=255)                                                                                                                                                                                                                                                              g‑ccCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCag  >  1:440867/1‑250 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                          ATAATTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGA‑CCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTGCT‑CGCGTTCCG‑CCG‑CCCGTG‑C‑TTTAACCGTACCCA‑GGCGGG‑CAG‑TAA‑CCTCCGCCCGATTCTTT‑CCCCAG‑ATATCGA‑G‑AT‑GCCA‑GCCCGCCGTTAAGC‑CAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATC‑GTTCAGA‑GCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAG  >  NZ_CP009273/2221469‑2221967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑