| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,428,361 | 0 | T | G | 32.4% | 160.8 / 11.3 | 75 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase TruA |
| Reads supporting (aligned to +/- strand): ref base T (8/42); new base G (24/0); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGA > NZ_CP009273/2428116‑2428605 | tAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTGGGAGATCATACCGGGCCGGGTaaag > 2:87376/1‑248 (MQ=255) aGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAAACCa > 2:630579/1‑250 (MQ=255) aGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATcc < 2:412841/249‑1 (MQ=255) gAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCAc > 1:143810/1‑250 (MQ=255) aaCAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTGCGGGGAAtacccc > 2:952229/1‑245 (MQ=255) aaaaTGGCATAAACCCAGACGCCGTACTCCGCGTCCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgcg < 2:589613/250‑1 (MQ=255) aaaaTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgcg < 2:357066/250‑1 (MQ=255) aaaTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAATAAAACGGTGAGGGGAAAACACcccgg > 1:856510/1‑249 (MQ=255) aTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTATCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGATAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTGGGAGAATAAAACGGTCAGGGGTATACCCcgcgcc > 2:1008421/1‑247 (MQ=255) ggCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATTCACCGCGAc > 1:708255/1‑247 (MQ=255) aaCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa < 1:46117/249‑1 (MQ=255) ccAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCCTAACCGGCCGGGGAATCCACCCCGCCCCAATACAGc‑cc > 1:826842/1‑250 (MQ=255) aGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGACATAGCGGGCCCATTGGCGGTTTTTGGAGAATATACCAGTCAGTGTGATTCACCACGCCCCAAGACAAC‑CCtc > 2:283582/1‑249 (MQ=255) gCCGTACGCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGc < 2:560534/250‑1 (MQ=255) ccGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCt < 1:155378/250‑1 (MQ=255) aCTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCCGGGGAATCCACCGCGACCAGATAAAAC‑CCTTTCGCTTTTg > 1:608694/1‑250 (MQ=255) gcgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGtt < 2:182370/250‑1 (MQ=255) ccgACCAGCTCTTCCAGAAGCCCGTCAAGGTGCAGGATAAAATCGAAGAAGAAATAAATCATGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGtt < 2:41734/249‑1 (MQ=255) aGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGTCCGGGGAATACACCCCGACCAGATACAAC‑CCTTCCGCTTTTTCCGTTTCTgcc > 1:414529/1‑250 (MQ=255) ccAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGC‑CCTTCCGCTTTTTCCCTTTCTGCCCCCcgcg > 1:334686/1‑250 (MQ=255) aTGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt < 2:74774/250‑1 (MQ=255) aTGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt > 1:205963/1‑250 (MQ=255) tgCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccgcc < 2:301999/250‑1 (MQ=255) tggATAAAATCGTGGAGGTAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGTGTACAGCCTGAAATGTTCCGAGCATTATTCTCTGTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAGACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCGGACGCTTTTGAGTGTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGc < 2:857096/249‑1 (MQ=255) aTAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGGCAGGGGAAATCACCCCGACCCGAAAAAAC‑CCCTTCGCCTTTTCCGTTGCTGCCGCCCCCGGTCTGTCCttttgcccccgccac > 1:8048/1‑238 (MQ=255) aaaaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAAC‑CCTTCCGCTTTTGCCGTTGCTGCCCCCCGCGGTCTGTCCTTTTCCGCCAGCAActc > 1:942318/1‑250 (MQ=255) aaaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActc < 1:652358/249‑1 (MQ=255) cGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGCACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCGGGCAGGGGGATCCACCGCGCCCCGATACAGC‑CCTTCCCCTTTTTTCGTTTCTGTCCCCCGCGGTGTGTCCTTTTCCGCCAGCCACTCTGcgt > 2:873742/1‑248 (MQ=255) gATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGGTGATCATTCCGGGCCGGGCAATCCACCGCGACCAGATACCGC‑CCCTCCACTTTTTTCGTTGTTGTCGCCCGCGGTCTGTCCTTTTTCGCCAGCACctcctgcat > 2:733212/1‑244 (MQ=255) gAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAACATTACGGGGTGGGGAATCCCCCCCCACCCAATACAACTCCTCTCCCTTTTCCCG‑TGCCGCCCCCCGACTTCTTTCCTTTTCCGCCAGCCACTCTGCTATCCa > 2:109656/1‑250 (MQ=255) ggAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCACCCCGACCAGATACAGC‑CCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCAGCAAATCTTCTATCCCGc > 1:163344/1‑250 (MQ=255) aataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct < 1:634683/250‑1 (MQ=255) aatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAAACCAACGCGGACAAATACAGC‑CCTTCCCCTTTTTGCGTTTCTGGCGCCAGCGTTCTGTCCTTTTCCGCCCGCAACCCTTCTATCCCGGTCTcccg > 1:384049/1‑248 (MQ=255) aGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑g < 1:826588/249‑1 (MQ=255) tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACAGC‑CCTTCCCCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTCCGCCCGCAAACCTTCTATTCCACCCTCCCGCT‑GGTtg > 1:647581/1‑250 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTggg < 1:568841/250‑1 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCACCGCGACCCGATAAAGC‑CCCTCCGCTTTTTCCGTTTCTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTCCCGCT‑GGGTTTGGGc > 1:325260/1‑250 (MQ=255) tttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGc < 1:63324/249‑1 (MQ=255) tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCAGGGGAATTCCCCGCGACCCGAAACCAC‑CCCTTCGCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCCTTTCCGCCCGCAACCCCGCTATCCCGCCCCCCGGCTGGGTTGTggcgccccct > 2:874173/1‑242 (MQ=255) gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCCGGGGAATCCACCCCGACCAGAAAAAAC‑CCCTCCGCCTTTTCCGTTGCTTCCGCCCGCGGTCTGTTCTTTTCCGCCCCCAACTCTTCTATACAGCTCTCCGGCG‑TGTTGTGGGGA‑CCCACCTCCACCAGGcctgc > 2:151997/1‑246 (MQ=255) aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGAc < 1:87376/250‑1 (MQ=255) tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACaa < 1:140047/247‑1 (MQ=255) ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTcc < 2:327081/250‑1 (MQ=255) gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCt < 2:419499/250‑1 (MQ=255) cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATACCCGTCAGGGGAATCCACCCCGGCCCGAAACAGC‑CCCTCCGCTTTTTTCGTTGCTTCCCCCAGCGTTCTGTTCTTTTCCGCCCCCAACTCTGCTATCCACCTCTCCCGCC‑GGTTGTGGGGG‑CCCACTTCCCTCACGCTGCCGAGACTAtttccg > 2:949583/1‑246 (MQ=255) cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTg < 2:213479/250‑1 (MQ=255) tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGAc < 1:141865/249‑1 (MQ=255) cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATatga < 2:412943/250‑1 (MQ=255) ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATCCCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGt < 1:636458/249‑1 (MQ=255) gCTACAGCCTGAAATGTTCCGATCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTa < 2:470348/249‑1 (MQ=255) cTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACa < 1:788411/250‑1 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGC‑CCTTCCCCGTTTTCCCGTGCTTGCGCCCGAGGTCTTTCCCTTGTCCCCCGCAAACCTTCTTTCCAGCTCTCCCGCG‑GGGTGTGGGGA‑CCGACTTTCACCACGGTGCCGACAATCTTATTAGCCCTATGATGTACAAAACCATTCGCttt > 2:841562/1‑250 (MQ=255) ttCCGTGCATTATTCTCGTTAGCCCGCCAGAAATAGCGGGCCCATTGGCGTGTTTGGAAGATCATACCGGTCAGGGTAATCCACCGTGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACATCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTa < 2:899483/250‑1 (MQ=255) ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTa < 1:810639/250‑1 (MQ=255) tCCGAGCATTATTCTCGTTATTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTaa < 2:138760/250‑1 (MQ=255) cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCCCCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAat < 2:225222/249‑1 (MQ=255) ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACACTCCCGTCAGGGGAAACCAACCCGACCAGATAAAGC‑CCTTCCGCTTTTGCACCTGGTTCCCCCACCGCTCTGTCCTTTGCCCCCCGCAACTCTCCTATTCAGCCCTCCCGCC‑GGTTGTGGGCC‑CCCGCTTTCTTCCCGCTGACGCTCAAATTCCTTACCATATTATGTCCAAAAGCATTTGTTTTTatatacacc > 2:476336/1‑245 (MQ=255) ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATTATACCCGGCAGGGGAATCCACCCCGCCCCGGTACAGC‑CCCTCCGCTGTTTCCGTTGCCTCCCCCACCGGTCTTTCCCTTGCCCCCCCCAACCCTTCTACCCCGCCCCCCCGCC‑GGTTTTGGGCACCCCCCCTCCACCCGCCTG‑CGCCACAAATCCTGATCATATGATATACACAAACACTCGCTTTAattacatcaccccc > 2:142/1‑238 (MQ=255) aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAATATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAg < 2:889922/249‑1 (MQ=255) ggccGACAGAAATAGCGGGCCCATTGGCTGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGCCCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGa < 2:200319/248‑1 (MQ=255) ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGAcc < 2:163344/250‑1 (MQ=255) cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCg < 1:213142/250‑1 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTg < 1:486373/250‑1 (MQ=255) aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGAcgc < 2:205963/250‑1 (MQ=255) gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAAc < 1:709121/250‑1 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGtt < 2:76909/249‑1 (MQ=255) aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑ACTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACTTTACTATGCATAACGTTGCGCCAc > 2:784512/1‑250 (MQ=255) cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATACCGTTGCGCCACgg > 2:233890/1‑250 (MQ=255) aTACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACggg > 2:230668/1‑250 (MQ=255) aTACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACgg < 1:1008421/249‑1 (MQ=255) ccGGTCAGGGTAATCCACCGCGGCCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATTAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGt < 2:856510/249‑1 (MQ=255) ccGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGtt > 1:230970/1‑250 (MQ=255) gTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCgg < 2:786231/250‑1 (MQ=255) gTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCgg < 2:325260/250‑1 (MQ=255) cAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGa > 2:231908/1‑250 (MQ=255) | TAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGC‑CCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCT‑GGTTGTGGGCA‑CCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGA > NZ_CP009273/2428116‑2428605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |