| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,009,484 | 0 | A | G | 34.4% | 157.9 / 33.8 | 90 | D257G (GAT→GGT) | pqiB | intermembrane transport protein PqiB |
| Reads supporting (aligned to +/- strand): ref base A (11/48); new base G (31/0); total (42/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGAT > NZ_CP009273/1009243‑1009730 | cTGTTCCGTGGCTATCGGGTAGGTTCGGGTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAggg < 2:689532/249‑1 (MQ=255) ttCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCa < 2:745883/248‑1 (MQ=255) ccGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGcc < 1:512861/249‑1 (MQ=255) tGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTg < 1:338535/250‑1 (MQ=255) gCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGc < 1:494887/250‑1 (MQ=255) tATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGG‑GGGAGCTTTGATGTGGCGGGAGGGCTGGGTTTTGGGGAGGCCGTGGGAc > 1:512270/1‑250 (MQ=255) tCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCAcc < 1:194574/249‑1 (MQ=255) gggTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGCAGGTTTTTTGTGCCGGGAGGGCTGGGTTTTGGGGAGGCAGGGGCACCgg > 2:988285/1‑248 (MQ=255) tAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAAc < 2:666511/250‑1 (MQ=255) ggTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCttt < 2:737274/249‑1 (MQ=255) gAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGGGTGGGTTTTGGGCAGCCCGGGGGACCGGAAAAAGGCTTTG‑ttt > 1:95837/1‑250 (MQ=255) ccTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGG‑GTCAGCATTTGTGTGGCGGAAGGGCCTGAGTTTGGGGAGCCCGGGGCCCCGGAAACCACTTTTG‑tttttttttattg > 2:832857/1‑241 (MQ=255) ttCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCCTTTATGTGCCGGGAGGGCTGGGTTTTGGGCAGCCAGTGGCACCGAAAAAAGCTTTCG‑TTTTGTTTgttgat > 1:6396/1‑249 (MQ=255) ttCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGG‑GTCCGCTTTGATGTTGCGGGAGGGGTGGGTTTTGGGGAGCCAGCGGGACCGAAAACAGCTTTCG‑TTTTTTTTgatgat > 1:42507/1‑249 (MQ=255) acacAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGTTGTGCCGGGAGGGGTGGGTTTTGGGGGGCCAGTGGCGCCGAAAAAAGCGTTTG‑TTTTGTATGATGATTagaa > 2:417270/1‑248 (MQ=255) aaaaaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTTAGCTTTGATGTGCCGGGAGGGCTGGGTTTTGGGGAGCCAGTGGGGCCGAAAAAAACTTTTG‑GTTTTTTTGGTTATTAGAAGAGCAt > 1:764179/1‑250 (MQ=255) aaaaaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCAt < 1:611903/250‑1 (MQ=255) aaaaCGCAATATCAGCTATCCACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCAtt < 2:142949/250‑1 (MQ=255) aaaaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGGCGGGAGGGCTGGGTTTAGGGGAGCCAGGGGCACCGGAAACAGCTTTCG‑TTTTTTTTGATGATCAGAAGAGAAtt > 1:450867/1‑250 (MQ=255) aaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTTGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCa < 1:909751/250‑1 (MQ=255) aaCGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAGAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCa < 2:333926/250‑1 (MQ=255) gCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGTGGGGTTTG‑TGTGCGGGGGGGGGTGGGGTTTGGGGAGCCCGGGGCGCCGAAAACAGATTTTG‑TGTTGTGTGTTGTTTAGAAGAGcgattagg > 2:607816/1‑242 (MQ=255) gCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGa < 1:963733/250‑1 (MQ=255) aaTATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCTGCTTTTATTTGTCGGGAGGGCTGGGTTTTGGGCAGGCAGTGGGACCGGAAAAAGCTTTCGTTTTTGTTGGTTGTTC‑GAAGAGGCTTCAGGAtt > 1:262795/1‑250 (MQ=255) atatCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGTCGGAAGGGCTGGGTTTAGGGCAGCCAGTGGGACCGAAAAAAGACTTTG‑TTTTGTATGATGATTAGAAGAGCATTCAgggatt > 1:569523/1‑246 (MQ=255) atCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGTGGCCGGCTTTGTTG‑GCCGGGAGGGCTGGGTTTAGGGGAGCCCGTGGGACCCGAAAAAAATTTCG‑GTTTTTTTTGTGATCAGAAGAGCATTCAGGATTCGt > 1:577948/1‑250 (MQ=255) aGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGACGGAAGGTCTGGGTTTAGGGCAGGCAGTGGGACCGGAAAAAGATTTCG‑TTTTGTTTGATGATCAGAAGAGCATTCAGGATTCGTTGt > 1:397113/1‑250 (MQ=255) tATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCGTTTGTTTGCCGGGAGGGCTGGGTTTTGGGGAGGCCGGGGGACCGGAAACAGCTTTCG‑TTTTTTGTTTTGTGTAGAAGAGCATGCAGGATTCGTTTTaca > 2:378385/1‑250 (MQ=255) tCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACAcc < 1:600478/250‑1 (MQ=255) cTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATc < 2:26005/250‑1 (MQ=255) cTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGCCGCGTTGATGTGCCGGAAGGGCTGGGTTTTGGGGCGGCAGGGGGACCGGAAACAGCTTTTG‑TTTTTTTTGGTTATCAGAAGAGGATTCAGGATTCGGTGTACACCGAt > 1:501735/1‑249 (MQ=255) cACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCt < 2:45713/250‑1 (MQ=255) aCCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGGAGCTTTGATGGGGCGGGAGGGCTGGGGTTATGGGAGCCAGGGGGAACGGAAAAAGATTTTG‑TTTTGTTTGGTGAACAGAAGAGCATTCAGGGATTGGTGTAAACCGATCACATTGATTATTTg > 1:389430/1‑250 (MQ=255) cgtATGACCGACTGGTGACCGACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGAt < 2:696892/248‑1 (MQ=255) tATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGCAGCCTTTATGTTCCGGGAGGGCTGGGTTTTGGGCCGCCCCGGGCCCCGCAAACAGCCTTCG‑GTTTGTATGTTGGTCAGAAGAGCACTCCCGCATCGTTGTTACCCCCCCACATTTATTATTTGATGt > 2:807808/1‑250 (MQ=255) aTGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGt < 1:475739/249‑1 (MQ=255) ccGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGG‑GTCAGGTTTGTTTTGGCGGGAGGGGTGGGTTTTGGGGAGGCAGGGGCACCCAAAAAAACCTTTG‑GTTTTTTTGGTGGTTAGAAGGGCATTCAGGGTTTGGTTTTCACCGATCACATTTGTTTTATTATGtttttt > 2:23851/1‑250 (MQ=255) gACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTaa < 1:28701/250‑1 (MQ=255) gACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGGTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTaa < 2:807211/250‑1 (MQ=255) ccAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCGGCTTTTGTGTGCCGGGGGGGCTGGGGTTTTGGGAGGCCGTGGCACCGCAAAAAGCTTTTG‑TTTTTTTTTATGATCAGAAGAGCATTTCAGAGTCTTTGTTTACCGCTCACATTTTTTATTTTATGTTTTTTTTAGATTTGGt > 2:538361/1‑250 (MQ=255) aacaaTGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTAc < 1:481229/250‑1 (MQ=255) aacaaTGTTCGCTTCGGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTAc < 1:39252/250‑1 (MQ=255) ttCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCt < 1:793804/250‑1 (MQ=255) gCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCCGCTTTGGTGTGGCGGGAGGGCTGGATTTTGGGGAGCCCGGGGGCCCGGAAAACGCATTTG‑TTTTTTATTATGATTAGACGAGCAATCAGGATTTGTTGTTTTACCCTCACATTTGATATTTGATGTTCTTTTAAGTTTCGGGACGCGGGCTgt > 2:594857/1‑248 (MQ=255) tGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCTGCCATTATGTGACGGGAGGGACGGGTTTTGGGCAGGCAGTGGGGCCGCTAAAAGGTTTCT‑GTTTTTTTGTTTGTCAGGAAAGCATTTAGGGATCTTTGTACACCGATCACCTTGCTTTTCTGATGTTTTTTTTAGATTTGGTACGCGGTCTgtcacccg > 2:69756/1‑243 (MQ=255) gAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCTGTCTGCAACCGGGa < 1:266445/250‑1 (MQ=255) aTAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTcc < 2:425292/250‑1 (MQ=255) aGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGCGGCTTTTTTGTGCCGGAAGGGGTTGGTTTTGGGGCGGCAGGGGCACCGGAAAAAACTTTTG‑TTTTTTATGTTGATCCAAAGAGAATTCAGGAATCGTTGTTAAACGGTCACATTTGTTATTTGGTTTTCTTTAAAGATTTTGTTCGCGGGCTGCAAACGGGGGCTCCgg > 2:528421/1‑250 (MQ=255) gTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTTATGTGGCGGAAGGGCTGGGTTTTGGGGAGCCAGTGGCACCGAAAACAGCTTTTG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTAAACCGATCACATTTATTATTTGATTGTCTTTAAAGATTCGGTACGCGGGCTGCAACCGGGGGCTCCGGt > 1:165444/1‑250 (MQ=255) gCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTaga < 1:32043/250‑1 (MQ=255) acttGCGGTTGATCTGACGTCAGGAGTGATGGGTGTGGAGATGGGCTCAATGACAACTCTGCTGAGTGGCGGT‑GTCGGCTTGGATGTGCCGGAAGGTTTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCTTTGTACACCGATCGCATTGATTAGCTGATGTTCTTTGAAGATTCGGTACGCGGTCTGCAGCCGGGAGCTCCGGTagag < 2:352202/248‑1 (MQ=255) cttGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGAGTTGTTGTGCCGGAAGGTTTGGGTTTTGGGGAGCCAGGGGCCCCGACAACAGCTTTTG‑TTTTTTATGGTTATTAAGAGAGGATTATGGATTCGTTGTAATCCGATCACCTTGTTTTTGTTATTTTCTTTTAAGATTTGTTTAGCGGTCCTCAACCCGTGGCTCCGGtgtacg > 2:519688/2‑245 (MQ=255) ctgTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCg < 2:509466/248‑1 (MQ=255) ggTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCgg < 1:322855/250‑1 (MQ=255) gACGTCAGCAGGGATGCGTGTGGAGATGGTCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGc < 2:819830/250‑1 (MQ=255) gACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGC‑GGTCGGATTGTTGTGCCGGGAAGGCTGGGTTTTTGGCAGCCAAGGGGCCCGAAAAAAACTTTTG‑TTTTTTTTGATGATTAGGAGAGACATCAGGATTCCTTTTACACCCGTCAACTTTCTTATCTTAAGTTTTTTTTAGATTCGGTCCGCGGGCTGCAACCGGGAGCTCCCCGAGAGGTCCGGGGGGTTCGc > 2:314786/1‑250 (MQ=255) aCGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTTTTGTGGCGGAAGGGCTGGGTTTTGGGGAGCCAGGGGGACCGAAAACAGCTTTCG‑TTTTTTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTTATTATCTGATGTTCTTTTAAGATTCGGTACGCGGGCTGCAACCGGGAGCTCCGGGAGAGTTTCGGGGGGTTCGcc > 1:736165/1‑250 (MQ=255) aCGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCg < 2:753210/248‑1 (MQ=255) cGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GGCAGCGTTTATGTGGCGGAGGGGCTGGGGTTAGGGGAGCCAGTGGGACCGGAAACAGCTTTCG‑TTTTGTATGGTGATTAGAAGAGCATTCAGGAATCGTTTTAAACCGGTCACATTGATTTTTTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACAGGGAGCTCCGGGGGAGGTGCGGGGGATTCGGCt > 2:637419/1‑250 (MQ=255) gTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTg < 2:30877/250‑1 (MQ=255) tCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTTATGTGGCGGGAGGGCTGGGTTTTTGGGAGCCAGGGGGACCGAAAAAAGCTTTCG‑TTTTTTATGATGATCAGAAGAGCATTCAGGGTTCGTTGTTCACCGATCACATTGATTATTTGATGTTTTTTTAAAATTTGGTACGCGGTCTGCAACCGGGGGCTCCGGGAGGGGTTCGGGGGATTTGCCTgg > 1:103849/1‑250 (MQ=255) gggATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTTTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTTTCTGATGTTCTTTTAAGATTCGGTACGCGGTCTGCAACCGGGGGCTCCGGTAGAGTTCCGGGGGATTCGCCTGGGTACCg > 1:229534/1‑250 (MQ=255) aTGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTaa < 1:159138/250‑1 (MQ=255) cGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGGAGGTCTGGGTTTTGGGCAGCCAGGGGCACCGAAAAAAGCTTTCG‑TTTTTTATGATGATTAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTTTCTGATGTTCTTTTAAGATTTGGTACGCGGGCTGCAACCGGGAGGTCCGGTAGAGTTCCGGGGGATTTGCCTGGGTACCGTAAGc > 1:35257/1‑249 (MQ=255) gtgtGGAGATGGGCTCATTGACAACGCTGCTGAGTGGTGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCa > 2:341798/1‑249 (MQ=255) tgtgGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTTAGCTTTGATGTGCCGGAAGGTCTGGGTTTTGGGGAGCCAGTGGGACCGGAAAAAGCTTTTG‑TTTTTTATGATGATCAGAAGAGCATTCTGGATTCGTTTTTCACCCATCACATTTATTTTTTGATGTTCTTTTAAAATTTCGGACGCGGGCTTGCACCCGGAGCTCCGGTAGAGTTTCGGGGGATTCGCCTTGGGAACGTGAGCaa > 1:872158/1‑249 (MQ=255) tgGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAg < 2:676131/249‑1 (MQ=255) cATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTTATGTGCCGGGAGGGCTGGGTTTAGGGCAGCCAGTGGCACCGAAAAAAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTTAAGATTCGGTACGCGGTCTGCAACCGGGGGCTCCGGGAGAGGTCCGGGGGATTCGCCTGGGGACCGTAAGCAAAGTGCCATTCTTTgc > 1:266943/1‑250 (MQ=255) aCGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAata < 2:801241/250‑1 (MQ=255) cGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAatat < 1:147612/250‑1 (MQ=255) ctgctgAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTTTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGc < 2:688484/250‑1 (MQ=255) ctgAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt < 1:68998/249‑1 (MQ=255) ctgAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTc < 1:917189/250‑1 (MQ=255) aGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAg < 1:741258/249‑1 (MQ=255) tGGCGGT‑GTCAGCTTTGATGTGCCGGGAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACa > 1:566698/1‑250 (MQ=255) tGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACa > 1:119475/1‑250 (MQ=255) gCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACAtt < 1:519199/250‑1 (MQ=255) gCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACAtt < 2:266943/250‑1 (MQ=255) tCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGGTTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACga > 2:612393/1‑250 (MQ=255) tCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACga > 1:158148/1‑250 (MQ=255) aGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgat < 1:27854/249‑1 (MQ=255) gCTTTGATGTGCCGGAAGGTCTGGATTTAGTGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga < 2:193552/250‑1 (MQ=255) gCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga > 2:26353/1‑250 (MQ=255) tttGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatgat > 1:557429/1‑249 (MQ=255) tGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTa < 2:764179/249‑1 (MQ=255) aTGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCg < 1:230348/250‑1 (MQ=255) ccGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt < 2:535753/249‑1 (MQ=255) ccGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTc > 2:489389/1‑250 (MQ=255) ccGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCC‑GGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTAACGTCTTcc > 2:631442/1‑250 (MQ=255) gATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGAt < 1:659808/248‑1 (MQ=255) | CTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGATAGTGGCATTGCGGTTGATCTGACGTCAGCAGGGATGCGTGTGGAGATGGGCTCATTGACAACGCTGCTGAGTGGCGGT‑GTCAGCTTTGATGTGCCGGAAGGTCTGGATTTAGGGCAGCCAGTGGCACCGAAAACAGCTTTCG‑TTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGAT > NZ_CP009273/1009243‑1009730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |