Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,322,608	A→C	V164G (GTG→GGG)	pmrB ←	two‑component system sensor histidine kinase PmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,322,608	0	A	C	100.0%	314.5 / NA	81	V164G (GTG→GGG)	pmrB	two‑component system sensor histidine kinase PmrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (51/30);  total (51/30)

CGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGT  >  NZ_CP009273/4322363‑4322852                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     cggcgCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGcccccc                                                                                                                                                                                                                                                  <  1:159413/250‑1 (MQ=255)   gcgcTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGGCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCg                                                                                                                                                                                                                                                <  2:915882/250‑1 (MQ=255)         tctGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTg                                                                                                                                                                                                                                          >  1:342045/1‑250 (MQ=255)             gCAATAGCAGGGCTTGATGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTCCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCCCCAGTGGCgc                                                                                                                                                                                                                                      >  2:565831/1‑249 (MQ=255)               agaaGCAGGGTTTGTGGGCGCGGTGCGACCAGGGTATTGAGTTGTTCATAGGAGGGAAAAAGAACACCTCCCAGCAGTTTTCAATGTTGAAAATTCCCGAAAAAAAAT‑GTCTG‑CCC‑GGCCCGCCCCGGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGAAA‑ACCCCCCCCCGCCAGTGGCGTTc                                                                                                                                                                                                                                    <  2:336279/247‑1 (MQ=255)                     aGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                                                                                                                              >  1:900469/1‑250 (MQ=255)                          ttGCTGTCGCTGGCCGAGCATGGTCTGGATTTCGTCATAGGAGGGAAGAATGACCTCTTCCAGCATTTTTACATGTTGATAATCCCCGAAAAAAAAT‑GCCTG‑CCC‑GCCCCGCCCCAGTGGCAGCAGCTGGGAGCCTCTCCCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCTTTTTCGCAAGCATTTCCAGATGCAA‑ACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTg                                                                                                                                                                                                                          <  2:536915/249‑1 (MQ=255)                                    tGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAg                                                                                                                                                                                                               >  1:898278/1‑250 (MQ=255)                                     ggTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                                                                                                                              >  2:197104/1‑250 (MQ=255)                                         gAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGCGCCGTTCGCAGTTCGTGCGCGACGCCGGCGGt                                                                                                                                                                                                           >  2:956472/1‑249 (MQ=255)                                             aTGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaac                                                                                                                                                                                                       >  2:143195/1‑249 (MQ=255)                                              tGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaa                                                                                                                                                                                                     >  1:16617/1‑250 (MQ=255)                                                  aCTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACTCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTTCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCACCGCCAGTGGAGTTCGCAGTTCGTGCGCGACGACAGCGGTCAACAACCtt                                                                                                                                                                                                 >  2:252053/1‑250 (MQ=255)                                                         tCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTAt                                                                                                                                                                                          >  2:203824/1‑250 (MQ=255)                                                           gTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATc                                                                                                                                                                                         >  2:763586/1‑249 (MQ=255)                                                           gTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATc                                                                                                                                                                                         >  1:197587/1‑249 (MQ=255)                                                             cATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                                                                                                                      >  2:663598/1‑250 (MQ=255)                                                              aTACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCCGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGc                                                                                                                                                                                     >  2:57793/1‑250 (MQ=255)                                                                  gAGGGGAGAATGACTTCTCCCAGCAGTTTAACATGTGGAAAATACCAGGAAGAAAAT‑GCCTG‑TCC‑GCCGCGCGCAGGTGGCAGCAGCGGGGAGCTGCTCTCCAACATC‑TAACCAACCCGTCCCACTAAGGGTGCTACATCAATGGGATGCGTTTCCGCCAGAAGTCCCAGATGCAA‑CCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGc                                                                                                                                                                                 <  2:180963/250‑1 (MQ=255)                                                                  gAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGc                                                                                                                                                                                 >  2:819725/1‑250 (MQ=255)                                                                        gaaagaaCATTTTCCGACATTTTTACAGGTGAATATGTCCCGAAAGAAAAT‑G‑CCG‑TCCGGGCACGCGCCGGTGGCAGCGGCGGGGGGCCCCTCCCAACCTTC‑TAACCAGCCCGTGCCACTAACGGTGCTACATCATTGGGATGCGTTTTCGCCAGCAGTTCCAGATCAAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCa                                                                                                                                                                           <  2:407174/244‑1 (MQ=255)                                                                        agaATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGt                                                                                                                                                                             >  2:235067/1‑248 (MQ=255)                                                                                atcacccAGCAGTTTTACTTGTTAATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCGGTTGCAGCAGCGGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCATTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGAAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                                                                                                                   <  1:435531/245‑1 (MQ=255)                                                                                aTCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg                                                                                                                                                                   >  2:873248/1‑250 (MQ=255)                                                                                 tCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGa                                                                                                                                                                  >  2:99153/1‑250 (MQ=255)                                                                                  cTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAc                                                                                                                                                                 >  1:692389/1‑250 (MQ=255)                                                                                           gTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                                                                                                                        >  2:944558/1‑250 (MQ=255)                                                                                            ttttACATGTTGATAATTCCCGAAAAAAAAT‑GACTG‑TCC‑GGCACGCCCCAGTTGCAGCAGCTGGGAGCCGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGtt                                                                                                                                                        <  1:273720/249‑1 (MQ=255)                                                                                             tttACATGTTGATAATTACCGGAAGAAAAT‑GATTG‑TCC‑GGCATGCGCCAGTTGCAGCAGCTGGGAGACGCTCCCCATCATC‑CGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCCA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGAGCGACGTCAGCGGTAAACCACCTTTTGTTATTCAGCGTGCTGGTCAGGCGCCTGACCAGCTCGttt                                                                                                                                                       >  2:385537/1‑249 (MQ=255)                                                                                               taaattttGAAAATCACCAGAAGAAAAC‑G‑CTGCTCC‑GGCCCGCGCCTGTGGCCGCGCGTGGGAGACGCTTCCCATCACC‑TGATCAAGCCGTCCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑CCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTaa                                                                                                                                                     <  2:167822/243‑1 (MQ=255)                                                                                               tACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAAc                                                                                                                                                    >  1:257796/1‑250 (MQ=255)                                                                                                    gTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg                                                                                                                                                >  1:244807/1‑249 (MQ=255)                                                                                                      tgaaaatccccGGAAAAAAAG‑CACTG‑CCC‑GGCACGCGCCGGTTGAGGCAGCGGGGGGCCGCTCTCCAACATC‑TGAACAAGCCGTGCCACTAAGGGTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTCCCAGATGCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                                                                                                                              <  2:112865/240‑1 (MQ=255)                                                                                                      tGAAAATTTCCCGAAAAAAATCGCCTG‑CCC‑GGCGCGCCCCGTTGGCCACGGCGGGGAGCCCCTCCCCTTCTTC‑TGAACAAGCCGTGCCACTACCGGGCCTACATCAATGTGTTGCGTTTCCGCCGGCAGTCCCAGATGCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                                                                                                                              <  2:326087/250‑1 (MQ=255)                                                                                                        aTAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAac                                                                                                                                            >  2:228188/1‑249 (MQ=255)                                                                                                         tAATTACCGGAGGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacca                                                                                                                                          >  1:787134/1‑250 (MQ=255)                                                                                                         aattcacggaaagaaATT‑GCCTG‑TCG‑GGCCCCCGCC‑GTTGGCGCCGGTGGGGGGCCCCCTCCCTCCTCTTGTCCAAGCCGGCCCACTACCGGGGCTACATCAATGTGTTGCTTTTCCGCCAGCATTCCCAGATGCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacct                                                                                                                                          <  2:534686/240‑2 (MQ=255)                                                                                                               ccGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCTCtt                                                                                                                                    >  2:233059/1‑250 (MQ=255)                                                                                                                  gaagaaAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGa                                                                                                                                 >  1:771575/1‑250 (MQ=255)                                                                                                                    agaaAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAtt                                                                                                                               >  2:849223/1‑250 (MQ=255)                                                                                                                     gaaAAG‑GACGG‑TCC‑GCCCCGCCCCGGTGGCAGCAGCTGGGAGACGCCCCCCATCATC‑TAATCAAGCCGGGCCACTAACGGTGCTACATCAATGTGATGCGTTTCCGCCAGAAGTTCCAGATGAAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt                                                                                                                              <  1:151842/250‑1 (MQ=255)                                                                                                                        aaT‑GACTG‑TCC‑GGCCCGCGCCAGTTGCAGCAGCGGGGAGACGCTCCCCATCATC‑TGATCAGCCCGTCCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGa                                                                                                                           <  1:641531/250‑1 (MQ=255)                                                                                                                         aT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAg                                                                                                                          >  2:696491/1‑250 (MQ=255)                                                                                                                              cTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTg                                                                                                                      >  2:258077/1‑250 (MQ=255)                                                                                                                                  ccc‑GGCACGCGCCAGTGGCAGCGGCGGGGAGCCGCTCCCCATCATC‑GGATCAAGCCGTCCCACTAACGGTGCTACATCAATGTGGTGCGTTTCCGCCGGCAGTCCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg                                                                                                                   <  1:579107/249‑1 (MQ=255)                                                                                                                                       gcccgcgcgAGTTGCAGCAGCGGGGAGACGCTCCCCATCATC‑TAATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCCCCAGCATTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgt                                                                                                               <  1:74326/247‑1 (MQ=255)                                                                                                                                          cgcgCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGCTTTCGAGGGTGGCGCTGTGaa                                                                                                            >  2:608182/1‑250 (MQ=255)                                                                                                                                             gcCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGCGGCGCTGTGAATgg                                                                                                         >  2:651753/1‑250 (MQ=255)                                                                                                                                                          gcTGGGAGCCGCTCCCCATCATC‑TGATCAAGCCGTCCCACTAACGTTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGCAA‑ACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTaa                                                                                            <  1:491106/250‑1 (MQ=255)                                                                                                                                                              ggacccGCTCTCCATCATC‑TGACCAACCCGTGCCTCTAACGGTGCTCCATCAATTTGAGCCTTTTCCGCCAGCATTCCCAGACCAAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTg                                                                                        <  2:78281/245‑1 (MQ=255)                                                                                                                                                                  aCGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACGTCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTcgg                                                                                    >  1:260077/1‑250 (MQ=255)                                                                                                                                                                       ctcCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTg                                                                               >  2:695032/1‑250 (MQ=255)                                                                                                                                                                        cccATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAAGGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTgc                                                                              <  1:50396/249‑1 (MQ=255)                                                                                                                                                                         ccATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTgcg                                                                             >  1:647541/1‑250 (MQ=255)                                                                                                                                                                            tcatc‑TGATCAAGCCGGGCCACTAACGGTGCTACATCAAGGTGATGCGTTTTCGCCAGCAGTTCAAGATGCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTgcgcgc                                                                          <  1:210795/250‑1 (MQ=255)                                                                                                                                                                             catc‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGGGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCt                                                                         >  2:848732/1‑250 (MQ=255)                                                                                                                                                                              atc‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGAGCGCGCtt                                                                        >  2:906520/1‑250 (MQ=255)                                                                                                                                                                                  tGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTc                                                                       >  1:224215/1‑248 (MQ=255)                                                                                                                                                                                  ggtccAAGCCGGGCCACTACCGGTGCTACAACAATGTGATGCTTTCGCCCCAGCATTCCCAGACCCAA‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTcc                                                                      <  2:214139/245‑1 (MQ=255)                                                                                                                                                                                    cacaaccggggCCACAACCGGCGCTACATCAATTTGTGGCGTTTCCGCCAGCGTTCCCAGATGAAA‑CCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCa                                                                     <  2:242845/238‑1 (MQ=255)                                                                                                                                                                                    aTCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGc                                                                   >  1:137046/1‑250 (MQ=255)                                                                                                                                                                                              gCCACTGACGGTCCACCATCAAGGTGACGCGTTTCCGCCGGCATTCCAAGACGCAC‑CCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCa                                                         <  2:536969/250‑1 (MQ=255)                                                                                                                                                                                               ccACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAg                                                        >  1:798100/1‑250 (MQ=255)                                                                                                                                                                                                    aaCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCg                                                   >  1:874503/1‑250 (MQ=255)                                                                                                                                                                                                         tGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGTGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgc                                              >  2:278652/1‑250 (MQ=255)                                                                                                                                                                                                          gCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcg                                             >  2:322081/1‑250 (MQ=255)                                                                                                                                                                                                           ttcgaTCAATGTACTGCGTTTTCGCCAGCTGTTCCAGATGAAA‑AGCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCTCCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgg                                            <  1:582803/246‑1 (MQ=255)                                                                                                                                                                                                               aTCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTGCGCCAgcgggcgg                                        >  1:439236/1‑250 (MQ=255)                                                                                                                                                                                                                  aaTGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCCTTTTGCAGCTCCGCCACgcggcggggg                                     >  2:861022/1‑248 (MQ=255)                                                                                                                                                                                                                   aTGTGATG‑GTTTTTGCCATTCGTTCCCGATTCAACCCGCCCCACCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAGCCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGa                                    <  1:624050/250‑1 (MQ=255)                                                                                                                                                                                                                    tgtgATGCGTTTTCGCCAGCATTCCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGAt                                   <  1:170553/250‑1 (MQ=255)                                                                                                                                                                                                                    tggtggggtttttCGCCAGCATCTCCAGATACAT‑ACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGAt                                   <  2:895224/241‑1 (MQ=255)                                                                                                                                                                                                                    ggtgATGCGTTTTCGCCAGCGGTCCCAGATGCAA‑CCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGAt                                   <  1:816831/249‑1 (MQ=255)                                                                                                                                                                                                                      tgATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑CCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATgc                                 <  1:876256/250‑1 (MQ=255)                                                                                                                                                                                                                      ggATGTGTTTCCGCCAGCTGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATg                                  <  2:878184/248‑1 (MQ=255)                                                                                                                                                                                                                            gTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCCGCTCCGCCAGCGGGCGGGGGATGCGGCGTa                           >  2:333776/1‑250 (MQ=255)                                                                                                                                                                                                                               ttCGCCAGCAGTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGGGCGCTTTCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGGGATGGCGCGTACCg                        >  2:535967/1‑250 (MQ=255)                                                                                                                                                                                                                                    cagcagTTCCAGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTCATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGa                   >  1:726299/1‑250 (MQ=255)                                                                                                                                                                                                                                              aGATGCAA‑ACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATa          >  2:468419/1‑249 (MQ=255)                                                                                                                                                                                                                                                     aaccGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCg   <  1:10001/247‑1 (MQ=255)                                                                                                                                                                                                                                                        cGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGTTCAATAGCGt  >  1:97426/1‑248 (MQ=255)                                                                                                                                                                                                                                                        cGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGt  >  1:597637/1‑248 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     CGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAT‑GACTG‑TCC‑GGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATC‑TGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAA‑ACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGT  >  NZ_CP009273/4322363‑4322852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑