| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,615,375 | 0 | A | C | 34.7% | 155.3 / 18.2 | 75 | V7G (GTG→GGG) | eamA | O‑acetylserine/cysteine exporter |
| Reads supporting (aligned to +/- strand): ref base A (45/4); new base C (0/26); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.71e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCC > NZ_CP009273/1615130‑1615621 | ccAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACAcccc > 1:747273/1‑250 (MQ=255) ggCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTcgcg < 1:267884/250‑1 (MQ=255) cATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGa > 1:520129/1‑249 (MQ=255) aTACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACa > 1:233481/1‑250 (MQ=255) gTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCg > 1:533376/1‑250 (MQ=255) tAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGcc > 1:632771/1‑250 (MQ=255) aaTGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCct > 1:375568/1‑250 (MQ=255) aaaaaaGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCCTTTCGCGACATTCTACCGCCTCTGAAttt > 2:319657/1‑250 (MQ=255) agaaaagCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCt > 2:457909/1‑250 (MQ=255) aaagacAAACGGCGAAAAACGGATGGTAAACCCATACCCCAGCAGAAAATTGAGTGATACTTTGGTTCGGGCGAAAAAAAAGAAAGCGGAAAAAGCGACAAGCATAAAGCACAAACCGGCCAGCATCGGCGGGGGCATGTTATGAGCCCCCACTTTGATGACCACAAA‑ATTGACCCCCCATACGACCACTACC‑AGTAGCTCCACCCCCCCTTTTTTCCGCAACATTCTACCGCCTCTGAATTTCATCttt < 2:502440/245‑1 (MQ=255) aaagCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt > 1:585987/1‑250 (MQ=255) gCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGt > 1:236330/1‑249 (MQ=255) cgcAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATc > 1:741920/1‑249 (MQ=255) aaaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACt > 1:819062/1‑250 (MQ=255) aaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACt > 1:457400/1‑249 (MQ=255) aaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTa > 2:800599/1‑250 (MQ=255) tgaagggTTAACCAATCCCCCAGCACAAAATTCGGTGGTACTTCCGGTCGTGCAAAAAAAAAAAGAGCCGAAAAATCGACCAGCAAAAAGCGAAACCCGGCAAGCATCAGCGGTGGCATTTTATGAACCCCCACTTAGATGACCACAAA‑ATTTACCCCCCATACGCCCACTCCC‑AGTAGCCCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGc < 2:872653/245‑1 (MQ=255) tGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt > 2:502696/1‑250 (MQ=255) gATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt > 1:631587/1‑249 (MQ=255) tgccATACCCCAGAAGCAAATTAAGGGTGTGTTACGGTCGGGCGAAAAAAAAAAAGGCCGGAAAGGCGCCCAGCAAAAGGCGAAACCCGGCCGCCAAAAGGGGGGACATGTTATAAGGCCCCACTTTATTACCCAAAAA‑ATTTACCCCCCAAACACCCCCTCCC‑AGTAGCCCCAACCCCCCATCTTGCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt < 2:858619/248‑1 (MQ=255) acccATACCCAAGAAACAAATAAAGGGGTCCTTGCGGCCGGCGGACAAAAAAGATAGCCGGAAAGGCGACCACCAAAAAGCGCAAACCGGCAAGCATCAGCGGTGCCATTTTATGAACCCCCACTTTGATGACCACAAA‑ATTTACCCCCCATACGACCCCTCCC‑AGTAGCCCCAACCCCCCATCTTTCCGCGACATTATACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt < 2:635353/248‑1 (MQ=255) aTACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTc > 2:284126/1‑250 (MQ=255) cccAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCttt > 1:370164/1‑249 (MQ=255) cccAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCttt > 1:605370/1‑249 (MQ=255) ccAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATAGGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa > 1:589249/1‑250 (MQ=255) agcaacAAATTAAGTGGTACTTTCGGTCGTGCAACAAAAAAGATAGCCGAAAAAGCGCCCACCATAAACCGCAAACCGGCCAGCATCAGCGGTGCCATGTTATGAACCCCCCCTTTGATAACCACAAA‑ATTTACCCCCCATACGCCCCCTACC‑AGTAGCCCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACa < 2:108356/250‑1 (MQ=255) tagcaAATACAGTGGACGTTTCGGTCGTGATACAAAAAAAACAGCGGAAAAAGCACCAAACAAAAAGCGAAACCCGGCCAGCATCAGCGTGGGCATGTTATGAACCCCCCCTTTAATGCCCACAAA‑ATTTACCCCCCATCCGACCACTCCC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGt < 2:847979/249‑1 (MQ=255) gcaAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCAAAAA‑ATTTAGCCCCCATACGACCACTCCC‑AGTAGCGCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTg < 1:201203/250‑1 (MQ=255) caAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAACGACCAGGATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCACCATACGACAACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGttaa > 1:771974/1‑248 (MQ=255) aaTTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGAtt > 2:858417/1‑250 (MQ=255) ttCGGTGGTACTTTCGGTCGTGCGAAAAAAAAGAAAGCCGAAAAAGCGCCCAACAAAAAGCGAAAACCGGCCAGCATCAGCGGTGGCAGTTTATAAAGCCCCACTTTGAGGACCAAAAA‑ATTTACCCCCCATACGACCACTACC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCg < 1:37005/250‑1 (MQ=255) aCTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGcc > 1:373711/1‑250 (MQ=255) tCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCaa > 1:857335/1‑201 (MQ=255) cGGTCGTGCAACAAAAAAGATAGCCGAAAAAGCGACCAGAAAAAAGCGCAAACCGGCCAGCACCAGCGGTGGCATGTTATGAACCCCCACTTTGATGACCAAAAA‑ATTTACCCCCCATACGCCCACTCCC‑AGTAGCGCCAACCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAAc < 2:857335/201‑1 (MQ=255) gtgtgacGATAAAAAAGATGCCCGGAACAGCGACAAGCAAAAACCTAAATCCGGCCGGCAAAGGCGGGGGTTTATTAGGAGGCCCC‑CCTTGAAGGCCACAAATATATA‑CCCCCAAGCCGCCCCTACCGAGTACCGCAACCCCCCTGGGTTTTCGGTACTTGTGTCCGCCGCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacg < 2:841532/244‑1 (MQ=255) gggCGACAAAAAAGATAGCCGAAAAAGCGACCAGAATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAACCCCCCCTTTGATGACCAAAAA‑ATTTAGCCCCCATACGACCACTCCC‑AGTAGCGCAAACCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacgacg < 1:239535/248‑1 (MQ=255) gcaaaaaaaaaGAGAGCCGGAAACGCGACCAGCAAAAACCGCAAACCGCCCAGCATCAGGGGTGGCATTTTAGGAACCCCCCCTTTGATCACCACAAA‑ATTTCCCCCCCATACTCCCCCTCCC‑AGTAGCCCCAACCCCCCATTTTTTCGCTACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGc < 2:23924/245‑1 (MQ=255) cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt > 2:620761/1‑249 (MQ=255) aaaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATg > 2:54063/1‑250 (MQ=255) aaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgc > 1:339837/1‑250 (MQ=255) aGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCACCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcgc < 1:444413/250‑1 (MQ=255) tAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCaaa > 1:57551/1‑250 (MQ=255) cgaaaaaGCGACCAGAAAAAACCCAAACCCGGCCAGCACCAGCGGTGGCATTTAATGAAGCCCCCCTTTGATGCCCAAAAA‑ATTTACCCCCCATCCGACCCCTCCC‑AGTAGCCCCAACCCCCCATTTTTCCGCGAAATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAg < 2:605370/247‑1 (MQ=255) aaaGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAATTAAGTCGc > 2:301800/1‑250 (MQ=255) cgcccAGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtata < 1:564551/247‑1 (MQ=255) gacaaacATAAACCGAAAACCGGCCATCAACAGCGGAGGCATTTGATAAACCCCCACTGTGTTAACCACAAA‑ATTTACCCCCCATACCACCACTACC‑AGGAACTCCAACCCCCCATTTTTTCGCGACATTCTGCCGCCTCTGAATTTCATCTTTTGGAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATAc < 2:286149/244‑1 (MQ=255) caaaaaaaaGCGCAACCCGGCCACCATCAGGGGTGCCATTTTATGAAGCCCCACTTTGATACCCACAAA‑ATTTACCCCCCACACGCCCACGACC‑AGTAGCGCCACCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc < 2:646071/244‑1 (MQ=255) tAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtt > 2:744395/1‑250 (MQ=255) aaGCACAAACCGGCCAGCAGCAGCGGTGACATGTTATGAAGCCCCACTGTGATGCCCACAAA‑ATTTAGCCCCCACACGCCCCCTACC‑AGTGGCGCCACCCCCCCATCTTTTCTCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtttt < 2:30411/250‑1 (MQ=255) acccGGCCAGCATCAGCGGTGGCATTTTATGAAGCCCCACTTTAATGACCACAAA‑ATTTACCCCCCATACGACCACTCCC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATg < 1:796510/248‑1 (MQ=255) aaCCGGCTAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATg > 2:223605/1‑250 (MQ=255) aaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATg > 2:749664/1‑250 (MQ=255) ggCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTAt > 1:524788/1‑250 (MQ=255) gCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTAtt > 1:342611/1‑250 (MQ=255) ccAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTAttt < 1:454230/250‑1 (MQ=255) aGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCt > 2:21893/1‑250 (MQ=255) aTGTTATGAACCCCCACTTTGATAACCAAAAA‑ATTTAGCCCCCATACGCCCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTaaaac < 1:862447/250‑1 (MQ=255) tATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTaaaacaaaa > 1:794901/1‑250 (MQ=255) tGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATACGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAaaacaaaaca > 2:188115/1‑250 (MQ=255) tGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAaaacaaaaca > 2:359527/1‑250 (MQ=255) tGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAaaacaaaaca > 2:275840/1‑250 (MQ=255) aaGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGCCCACTCCC‑AGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATa < 1:443290/250‑1 (MQ=255) ccccACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑CGTATCTCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTTAATTTCATCTTTTGTCCTCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAAc > 2:25940/1‑249 (MQ=255) cccACTTTGTGGACCACAAA‑ATTTCCCCCCCATACGCCCCCCACC‑ATCAGCCCCACCCCCCCATCTTTCCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGa < 2:501912/250‑1 (MQ=255) tttGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCCTTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACg > 1:286960/1‑249 (MQ=255) tgatgaCCACAAA‑ATTTACCCCCCATACGCCCCCTCCC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGc < 1:531533/248‑1 (MQ=255) gatgaCCACAAA‑ATGTAGCCCCCATACGACCACTACC‑AGTAGCCCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCAc < 1:754372/249‑1 (MQ=255) atccccACAAA‑ATTTCCCCCCCACCCGCCCACCCCC‑AGTCGCCCCAACCCCCCATTTTCCCCCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACt < 2:706442/245‑1 (MQ=255) aattttACCCCCCAAACAACCACTCCC‑TGAAGCCCCAACCCCCCATCTTTCCGCGACATTCGACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGgacaga < 2:537406/247‑1 (MQ=255) aTTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATATTTTGTAAGAAAGAAACTTCGATGAGTTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTATGACGGCATTAAGGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGgacagac > 1:438053/1‑249 (MQ=255) aTTTAGCCCCCAAACGCCCCCTCCC‑AGTAGCCCAAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGgacagaca < 2:747273/250‑1 (MQ=255) ttAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACaaa > 1:364231/1‑250 (MQ=255) cATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTa > 2:505957/1‑250 (MQ=255) c‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGTATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGctc > 1:743588/1‑250 (MQ=255) aaCCCCCCTTCTTTCCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGcc < 2:375568/250‑1 (MQ=255) | CCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAA‑ATTTAGCCCCCATACGACCACTACC‑AGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCC > NZ_CP009273/1615130‑1615621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |