breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
MC JC 2,278,396 (TTATCGCCT)2→1 coding (542‑550/1761 nt) yejM → LPS biosynthesis‑modulating metalloenzyme YejM
RA 4,323,070 G→A P10L (CCA→CTA)  pmrB ← two‑component system sensor histidine kinase PmrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 235892 270719–269666 33775–34828 85 [0] [47] 48 yafV–[BW25113_RS01325] yafV,ivy,fadE,lpcA,yafJ,dpaA,yafQ,dinJ,yafL,rayT,BW25113_RS01160,lafU,dinB,yafN,yafO,yafP,BW25113_RS01190,prfH,pepD,gpt,frsA,crl,phoE,proB,proA,BW25113_RS01235,ykfI,yafW,ykfH,ykfG,yafX,ykfF,BW25113_RS01265,BW25113_RS25425,BW25113_RS25430,BW25113_RS25435,yafZ,ykfA,perR,BW25113_RS01295,BW25113_RS01300,BW25113_RS01305,ykfC,[BW25113_RS01325]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 20563NA (NA)88 (0.960) 78/496 0.3 96.7% intergenic (‑55/+252) BW25113_RS00100/rpsT IS1‑like element IS1A family transposase/30S ribosomal protein S20
?NZ_CP009273 = 1767035 3 (0.030)coding (267/1266 nt) ydiN MFS transporter
* ? NZ_CP009273 = 2358910 (0.000)85 (0.930) 76/496 0.4 100% intergenic (+26/+15) BW25113_RS25420/yafV ISAs1 family transposase/2‑oxoglutaramate amidase
?NZ_CP009273 269666 = NA (NA)intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
* ? NZ_CP009273 360104 =41 (0.450)60 (0.650) 49/496 2.1 53.8% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 62 (0.680)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =19 (0.210)78 (0.910) 73/464 0.3 84.9% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 10 (0.120)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326119 (0.210)72 (0.840) 63/464 0.7 83.8% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 10 (0.120)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 1767027 =7 (0.080)86 (0.940) 74/496 0.4 92.5% coding (259/1266 nt) ydiN MFS transporter
?NZ_CP009273 = 3577555 NA (NA)intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/protein YrhD