Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,143	0	A	T	28.8%	120.3 / 11.1	55	intergenic (+16/+49)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base A (22/15);  new base T (0/15);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 3.68e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGA  >  NZ_CP009273/2144900‑2145391                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                   aCGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGtt                                                                                                                                                                                                                                                               <  2:99539/249‑1 (MQ=255) aCGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGt                                                                                                                                                                                                                                                                <  1:276992/248‑1 (MQ=255)  cgtaTGTCACTTAATCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGGAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATGCCATTGATCCTTTATTAGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑a                                                                                                                                                                                                                                                            <  1:1311217/247‑1 (MQ=255)        tCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTgg                                                                                                                                                                                                                                                      <  1:1271445/250‑1 (MQ=255)          aCTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGc                                                                                                                                                                                                                                                     <  2:1009641/249‑1 (MQ=255)          aCTTATTCTAACGGCATGTTTATGGGCGGAATCAGCAATGACCCTGGTATTGTGACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGc                                                                                                                                                                                                                                                     <  2:365622/249‑1 (MQ=255)              aTTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑t                                                                                                                                                                                                                                                 <  1:584775/248‑1 (MQ=255)               ttCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTg                                                                                                                                                                                                                                               <  1:495709/249‑1 (MQ=255)                    aCGGCAGGGTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTAGAAAAACAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTGTCCAAAAGAAGAA‑TCCGACGA‑AC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑ttg                                                                                                                                                                                                                                         <  2:944187/249‑1 (MQ=255)                     cGGCATGTTTAGTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑ttg‑tt                                                                                                                                                                                                                                      <  2:1000307/250‑1 (MQ=255)                      ggCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑ttg‑ttg                                                                                                                                                                                                                                     <  1:190010/250‑1 (MQ=255)                            tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATTTTCCAATGATTCTTTTTTTGTGCCCCCTCCCAAAAAAGAA‑AACGACGAGGC‑AAGGAGAAAAAATATTTGCCTAACTACACGTT‑TATTTATTTTTT‑AAAATGGCGG‑GTGAG‑atg‑ttttttttt                                                                                                                                                                                                                               >  2:64586/1‑243 (MQ=255)                                   gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGcc                                                                                                                                                                                                                       <  2:947108/249‑1 (MQ=255)                                                        gTATTGTAACAAACGGAGAGTATGGCCAGGAGTGAAAAAACAAGAAAGAAGATATTACAGACTGGTTGTAGACGCGGGGAAAAAAAATTTACGGCGTTTATACCATTGTTCTTTTATGGCCCCCCTACAAAAAAGAAGAA‑GCCGAGGA‑GA‑TAAGAGAAAAATTGGTTCGTTAACCAGCTTTTATTTTTTTTTTTT‑AACCGGGCGT‑TTAAT‑TTT‑TTG‑TCTCAAA‑CCGCCATGAGAAACTCACTCTGCCcac                                                                                                                                                                                                 <  2:88057/250‑1 (MQ=255)                                                                 cAAACGTAGAATATGGCAAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGTATGTATACGCGGGGGAATAAAATTTACGGCGTTTATACCATTGATCCTTTATTGGTCCCCTATCAAAAAGAAGAG‑GCCAACGA‑GC‑TAAGACAAAAATTAGTCCGCAAATCAGCCGTTATATTTATTGTTA‑AACCGGGCGT‑TTGAA‑TTG‑TTG‑TCTTAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt                                                                                                                                                                                        <  1:428718/250‑1 (MQ=255)                                                                           aaagggcagggAGTGAAAAATCAAAAAAAAAAATTTTTCAGTCTGGATGTATACGCGCGGGAAAAAAATTACCGGCGTTTATCCCATTAATCCTTTATTGGTCCCCTACCAAAAAGAAGAA‑GCCGACGA‑TC‑AAAGAGAAAAATTATTTCCCTAATCACCCTTTATATTTATTTTTT‑AACCGGGCGG‑TTGAA‑TTT‑TTT‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGttt                                                                                                                                                                              <  1:60609/241‑1 (MQ=255)                                                                                     gAGTGAAAAAACAAAAAAAAAGTAATTTCAGATGGGTTGTATCCGCGCGGGGATAAAATTTACGGCGTTTATCCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGAGGA‑GC‑AAAGAGCAAAATTATTTCGCTAATCAGCCTTTATATTTTTTTTTT‑AACCTGGCGT‑TTGAA‑TTT‑TTG‑TCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcatc                                                                                                                                                                    <  1:87482/250‑1 (MQ=255)                                                                                       gTGAAAAAAAAAAAAAGAAATTTTTTCAGCCTGGATTTATCCGCGCGGGGATAAATTTACCGGCGGTTATACCTTTGATCCTTTATTGGTCACTTATCAAAAAAAAGAA‑GCCGACGA‑GA‑TAAGAGAAAAATTAGTCCGTTAATCAGCCGTAATATTTTTTTTTA‑AAACGGGCGT‑TTGAT‑TTT‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                  <  1:436690/250‑1 (MQ=255)                                                                                                           attttgaagagTGGTTGTATACGCGGGGGAAAAAAATTTCCGGGGGTTATACCATTTATCCTTTATTGGTCACCTATCAAAAAGAAGAA‑GCCGAGGA‑GC‑TAAGAGAAAATTTTGTTCGATAAGCAGCCTTTATATTTATTTTTT‑AAACGGGGGG‑TTGAT‑TTG‑TTT‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCggg                                                                                                                                            <  1:1121168/243‑1 (MQ=255)                                                                                                                            tataCGCGGGGGAAAAAAATTGACGGGGGTATTACTTTTGTTCTTTTAGGGGTCCACAATCAAAAAGAAGAA‑GCCTACAA‑GC‑AAGGAGAAAATTTTGTTCACAAATCATCTGTTATATTT‑TTGTTTAAAACGGGGGG‑TTGAT‑TGG‑TTT‑TCTCCAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCtt                                                                                                                          <  2:710426/250‑1 (MQ=255)                                                                                                                                     gggaaaaaaaTTTAGGGGGTTTATACCTTTGTTCTTTTATTGGACA‑CTACCCAAAAAAAAAACGCAGAGAA‑GC‑AAAAAAAAAATTTTTTTCCCAAACCCCCTTTTATTTTTTTTTTTT‑AACCGGGGTG‑TTGAT‑TTG‑TTT‑TCTCAAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTc                                                                                                                 <  2:27051/244‑1 (MQ=255)                                                                                                                                         aaaaatTTTACGGCGTTTATCCATTTGATCCTTTATTGGTCCCATACCAAAAAGAAGAA‑GCCGACAA‑GC‑AAAGAGAAAAATTAGTCCTCTATTCAGCCTTTATTTTTTTTGTTA‑AAACGGGCGT‑TTGAA‑TTT‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGt                                                                                                             <  1:812939/248‑1 (MQ=255)                                                                                                                                           aaaaTTACCGGCGTTATTACTATTGTTCTTTTA‑TGGTCCCCTAACCAAAAGAAAAA‑AGCCACGA‑GCAAAAAAAAAAAATTATTTC‑CTAAACCACCTTTTTTTTTTATTTTT‑AAAACGGGGG‑TTGAA‑ATGTTTG‑TTTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGtaa                                                                                                           <  2:597786/250‑1 (MQ=255)                                                                                                                                            aaaTTTCCGGCGTTTTTACATTTATTCTTTTTTTGTTCACCTACCAAAAAAAAGAA‑CCCGACGA‑GA‑TAAGAGAAAAATTATTTCTCTAATCAGCCTTTAT‑TTTTTTTTTT‑AAACCGGGGGTTTAAA‑TTT‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGtaat                                                                                                          <  1:972112/250‑1 (MQ=255)                                                                                                                                                   gggggtttaacccATTGATCTTTTA‑TGGGCACCTTACAAAAAAAAAAAGGCCGACGA‑GT‑AAAGAGAAAAATTAGTTCGCAAATCAGCCTTTATTTTTTTTTTTA‑AAACGGGGGG‑TTAAT‑TTG‑TTG‑TCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCg                                                                                                    <  1:805841/238‑1 (MQ=255)                                                                                                                                                         ttATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAAAT‑GCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                             >  1:68123/1‑250 (MQ=255)                                                                                                                                                          tataCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑CTTAA‑TTG‑TTG‑TTTTCCA‑ACGCCATGAGAAACACACTTTGGCCACAGCGCCACTTGCAAAGATTTAACATCATTCCCACGCC‑C‑CGTATCCGACGGGCCGGCACAAAACT‑TCTTTAACAATTCCTGGAATCAATCGCGTTGTTc                                                                                            >  2:845009/1‑250 (MQ=255)                                                                                                                                                            taCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGGA‑TTT‑TTT‑TCTTTAA‑CCGCCATGAGAAACTCACTCTGCCCCCCGCCCCCGTGCCAACGTTTTATCATTATCCGCTCCCC‑C‑GCTTCCCGCCCGGCCGGACACAAACT‑GCTTTTACAACTCATGTAAAAAACAGAGTTGTTCCt                                                                                          >  2:726021/1‑250 (MQ=255)                                                                                                                                                               cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTTAA‑TTT‑TTT‑TCTTTCA‑CCGCCCTGAGAAAATCACTCTTCCCCCCGCGCCCGTGCCCACGTTTTTTCATCATCCGTTCGCTCC‑TTTTCCCCCCGGGCCGG‑CCAAAACA‑GCTTTTACAATTTAGGCAATAAATCCAGTTTTTTCTGCg                                                                                       >  2:948947/1‑250 (MQ=255)                                                                                                                                                                        tttATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTTAC‑ACCCCATGAGGAAATCACTCTGCCCACAACGCCCGAGTCAACGTTTTTTCATTATTCGCACCCC‑CGGTTTACC‑CCGCGCTGGACAAAAAAT‑TCTTTAACAAATTCGGTTATAAATCAAGTTTATGCTGCGGTTCTgccc                                                                              >  2:976979/1‑249 (MQ=255)                                                                                                                                                                         tttttgtcccccTATCAAAAAAAGAAA‑GCCGACGA‑AA‑AAAGAGAAAATTTGTTTCGATAACCAGCTTTTATTTTTTTTTTTA‑AACCGGGCGG‑TTAAT‑TTG‑TTG‑TCTACAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgcc                                                                               <  1:1414894/238‑1 (MQ=255)                                                                                                                                                                            ttgtcccccTACCAAAAAAAAGAA‑GCCGACGA‑GC‑AAAGAAAAAAATTATTTCGCTAATCACCCTTTATTTTTTTTTTTA‑AACCTGGCGT‑TTAAT‑TTG‑TTG‑TCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCa                                                                           <  1:242030/242‑1 (MQ=255)                                                                                                                                                                             tGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑GTGGA‑TTG‑TTT‑TTTTCCA‑CCCCCATGAGAAACTACCTCTGCCCACCGCGCCAGTGCCAACGGTTTATCATCATCCGTAGCCC‑C‑CTGTAACGCCGGGCAGGGCAAAAACT‑CTTTTTACAAATCAAGGAAAAAAACCAGACTTTCCTGCGTTGCCGCCGCCact                                                                         >  2:367418/1‑248 (MQ=255)                                                                                                                                                                                 cACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGTA‑ATG‑TTT‑TTTTCAA‑CCACCCTGAGAAACTCACCCTTCCCCCACCGCCATGGCCCACGTTTTTTCATCACCCCTACCCC‑C‑CTTTACCCCCCGGCTGGAGACAAAAA‑TCTCTATAAATTTAGGGATTATATCACGTTTTTCCTTCGTtgctgcgccgctgctat                                                                     >  2:1394077/1‑239 (MQ=255)                                                                                                                                                                                   catATCAAAAAGAGTAA‑GCAGAAGA‑GT‑AAAGAGAAAAATTAGTCCTCTAATAAGCGTTTATTTTTTTTGTTA‑AATCGGGGGG‑TTGAA‑TTG‑TTT‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTg                                                                   <  1:159316/248‑1 (MQ=255)                                                                                                                                                                                      aTCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTTCCCCCCGGCTGGACAAAAACT‑GCTTTAACAATTCCGGTAATAAAACGAGTTGTTCCTGCGTTTCTGCCCCCCGTCCATTTaa                                                                 >  2:312198/1‑249 (MQ=255)                                                                                                                                                                                         cAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCAGTTATATTTATTGTTT‑AAACTGGTGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCCCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAATAACT‑CCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTACGTTGTAAAAc                                                             >  2:1419059/1‑250 (MQ=255)                                                                                                                                                                                                agaa‑gCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTTAA‑CCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCCACGTTTTATCATCATTCCTACGCC‑C‑GTTTACCGCCCGGCCGGGCCAAAAAT‑GCTTTAACAATTCACGTCATAAATCCAGTTGTTCCTGCGTTGCGTCCCCCCGGCCCTTTAAAAACg‑gggga                                                      >  2:1403165/1‑247 (MQ=255)                                                                                                                                                                                                   a‑gCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTCCA‑CCGCCCTGAGAAAATCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCCTCCGTACCCT‑C‑GTTTTCCGCCGGGCCGGACAAAAACT‑GCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTTCGTTTCTGCCGCCCGTCCCTTGAAAAACG‑GGGTaaag                                                   >  2:1057409/1‑249 (MQ=255)                                                                                                                                                                                                      caaaaga‑GC‑AAAGAAAAAATTTATTTCGTAAACCAGCTTTTATTTTTGTTGTTA‑AAGCTGGCGG‑ATAAT‑TTG‑TTG‑TTTTAAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTaaagaaa                                                <  1:225334/245‑1 (MQ=255)                                                                                                                                                                                                        gacga‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TTTTCAA‑CCGCCATGAGAAACTCACTCTTCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACGCC‑C‑GTTTACCGCCCGGCTGGGCCAAAACT‑GCTTTAACAATTCAGGGAATAAATCGAGTTTGTCCTGCGGTGCTGGCCCCAGTCCATTTGAAAACG‑GGGGAAAGAAAgg                                              >  2:647918/1‑250 (MQ=255)                                                                                                                                                                                                            cgagataaaacaaaaaTTATTTCCATACTCGCCCTTTATTTTTTTTTTTT‑AACCT‑GCCG‑TTGAA‑TTT‑TTT‑TTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGca                                            <  1:213618/238‑1 (MQ=255)                                                                                                                                                                                                            a‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTAACGCCGGGCTGGACAAAAAAT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGGAAAGAAAGGcaca                                          >  1:1251812/1‑250 (MQ=255)                                                                                                                                                                                                                      gCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTTAA‑TTT‑TTT‑TCTTCCA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCCGGGCCAACGCTTTTTCATAATCCCTACGCT‑C‑GTTTACCGCCCGGCTGGACAAAAAAT‑GCTTTAACAATTCAAGTAATAAATCGAGTTGTTCCTTCGATGCTGCCCCCAGTCCATTGACAAACG‑GGGTAAAAAAAGGcaactcaag                                     >  2:1027579/1‑240 (MQ=255)                                                                                                                                                                                                                      gCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTTAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTTTCATCATCCGCACGCT‑C‑GTTTACCGCCGGGCTGGCCAAAAACT‑GCTTTAAAAATTTAGGTAATAAATCGAGTTTTTTCTGCGTTGCTGCCGCCCGTCCCTTGAAAAAAG‑GGGTAAAGAAAGGCACATaaggag                                   >  1:11924/1‑249 (MQ=255)                                                                                                                                                                                                                        aaaaTTAGTCCTAAAATCAGCCTTTAT‑TTTTTTTTTT‑AAACGTGGGG‑TTGAATTTT‑TTT‑TCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAAt                                <  1:759070/250‑1 (MQ=255)                                                                                                                                                                                                                              aGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTCAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAATGCCCACGATTTTTTATCATCCCTACGCA‑C‑CGTTACCGCCCGGCTGGGAAAAAACA‑GCTTTTACAATACAAGGTATAAATAGAATTGGTCCTGCGTTGCTGCCGCACCTCCATTTAAAAAAGCGGGAAAAAAAAGAAACAT‑AGGCGAAttcacc                           >  2:877597/1‑245 (MQ=255)                                                                                                                                                                                                                                   gCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTCCA‑CCGCCCTTAGAAAATCACTCTGCCCCCAGCGCCAGTGCCCACGTTTTTTCATCATCCGTACGCC‑C‑CTTTACCGCCGGGCTGGGCAAAAAAT‑GCTTTAACAATTCTGGTAATAAATCGCGGTGTTCCTGCGTTGCTTCCGCCAGTCCCTTGAAAAACG‑GGGTAAAGAAATGGACATTAGGGACATTATCCCGCata                     >  2:1287147/1‑250 (MQ=255)                                                                                                                                                                                                                                    cTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTT‑TTT‑TCTTTAA‑ACCCCCTTAGAAACTCACTCTTCCCCCAGCGCCAGTCCCCACGGTTTTTCATCATCCCTTCGCC‑C‑CTTTTCCCCCCGGCTGGGCAAAAACT‑GCTTTAAAAAATCAAGTAATAAATTGAGTTGTTTCTGCGCTGCTGCCGCCCCTCCACTGTAAAAAA‑GGGTAAAGAAAGGCCCACAAGGAGAATTATTCACAatat                    >  2:373855/1‑250 (MQ=255)                                                                                                                                                                                                                                     tAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGGA‑TTG‑TTG‑TCTTCAA‑CCGCCCTTAGAAAATCACTCTGCCCACCACGCCAGTGGCAACGTTTTATCACCATCCGGCCCCT‑C‑GTTTACCGCCGGGCTGGACAAAAAATGGTTTTAACATTTC‑GGGAATAAATCGAGGTGGTCCTGAGGTGCTGCCCCCCATCCCTTGTCAAAAG‑GGGTAACGAAAGGGACATTAGGAGAAttcttcaacctata                   >  2:1291314/1‑238 (MQ=255)                                                                                                                                                                                                                                      aaTCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑GCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATTATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAAAT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAAGCACATGAGGAGAATTATACAGCATAtt                   >  1:1218881/1‑249 (MQ=255)                                                                                                                                                                                                                                       aatcatccttATTTTTTTTTGTT‑AAACCGGGGG‑GTATA‑TTT‑TTGTTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat                    <  1:1346431/240‑1 (MQ=255)                                                                                                                                                                                                                                            cttttATATTTGTTGTAA‑AAACGGGCGG‑TTAAT‑TTG‑ATG‑‑CTAAAATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GATTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc            <  1:535403/247‑1 (MQ=255)                                                                                                                                                                                                                                            ctgTTATTTTTTTTGTTA‑AACCGGGGTG‑TTGAA‑TTG‑TTT‑TCTCCAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAc               <  1:489821/245‑1 (MQ=255)                                                                                                                                                                                                                                               ttATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTTAA‑CCGCCCTGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCT‑C‑GGTTTCCGCCGGGCCGGAAAAAAAAT‑GCTTTTAAAAATCAGGGAATAAAACGAGTTGTTCCCGCGGTGCTGCCGCCAGGCCCTTGAAAAAAG‑GGGGAAAGAAAAGCACATGAGGAGAAATATTCAGCATATTTTACGACAgg         >  2:352880/1‑249 (MQ=255)                                                                                                                                                                                                                                                  tatttattGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTT‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCCCACGCT‑C‑GTTTACCGCCCGGCTGGGCAAAAACA‑TCTTTAAAAATTCAGGTAATAAAAATAGTTGTTCCTGCGGTGCTGCCGCCCGTCCATTTTAAAAAA‑GGGTAAAAAAAAGGACAAGAGGAGAAATATACACCATATTTaaaccccgtggc      >  2:1395345/1‑240 (MQ=255)                                                                                                                                                                                                                                                   atttattGTTT‑AAACTGGCGG‑TTGGA‑TTG‑TTG‑TCTTTAA‑ACGCCATGTGAAACTCACTCTGCCCACAGGGCCAGTGGCAACGTTTTATCATTATTCGTACGCT‑C‑GTTTACCACCGGGGTGGAAAAAAACT‑GCTTTTACAATTCAGGTAATAAATCGAGGTGGTGCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGGAAAGAAAGGCACATGAGGAGGAATATCCAGCATATTTCACGCCAGTCGCt     >  2:337760/1‑250 (MQ=255)                                                                                                                                                                                                                                                      tattGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGGCAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCCCCAGTCCATTGAAAAACG‑GGGGAAAGAAAGGCACCTGAGGAGAATTATACAGCATATTTAACGCCAGTCGCTGGa  >  1:732379/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                   ACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAA‑GCCGACGA‑GC‑TAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTT‑AAACTGGCGG‑TTGAA‑TTG‑TTG‑TCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCT‑C‑GTTTACCGCCGGGCTGGACAAAAACT‑GCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACG‑GGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGA  >  NZ_CP009273/2144900‑2145391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑