Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,175,791 T→G F172V (TTC→GTC)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,175,7910TG99.3% 563.1 / NA 153F172V (TTC→GTC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base G (66/85);  minor base A (0/1);  total (66/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

CGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTC  >  NZ_CP009273/4175544‑4176037
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
 gTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCg                                                                                                                                                                                                                                                     <  1:236179/250‑1 (MQ=255)
    gAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGCACGTCAGCAGATCCTGACTGAAGAGCCGCATCTGGACGCGCTCGAAGAGGACGGt                                                                                                                                                                                                                                                   >  2:846021/1‑249 (MQ=255)
     aaGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGCGGTCGGTGa                                                                                                                                                                                                                                                 >  1:111679/1‑250 (MQ=255)
           cccAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAAtt                                                                                                                                                                                                                                           <  1:860159/250‑1 (MQ=255)
                    aaGAACGCCGCGAGGCAATGGCCCACGTCGACGTGGCCTCTCCGACTGCGCACAGCTGGTTCCTGAAATCTCTGCCGTCCCTTATCTGTCTGCTGCACGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTTGTTATCGAAGGCGGTATGACCAACCTTGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCTCTGTAAGAGGTCGGTGACTAATTCGACGCGa                                                                                                                                                                                                                                   <  2:369876/249‑1 (MQ=255)
                     aGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAg                                                                                                                                                                                                                                 <  2:1349774/250‑1 (MQ=255)
                      gTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATTACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGACGGCGACGAATTCGAcgag                                                                                                                                                                                                                                    >  2:1416210/1‑244 (MQ=255)
                            cgggAGCGTATGGGCCACATCGAACGGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATggggg                                                                                                                                                                                                                          <  2:851652/247‑1 (MQ=255)
                              tGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCg                                                                                                                                                                                                                        <  2:1185719/250‑1 (MQ=255)
                                  cGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAg                                                                                                                                                                                                                    <  1:425777/250‑1 (MQ=255)
                                      tGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCaa                                                                                                                                                                                                                 <  1:1130123/249‑1 (MQ=255)
                                        ggCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATcc                                                                                                                                                                                                              <  1:1070252/250‑1 (MQ=255)
                                            acaTCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGc                                                                                                                                                                                                          >  1:479110/1‑250 (MQ=255)
                                                       gCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAag                                                                                                                                                                                                <  1:850222/249‑1 (MQ=255)
                                                       gCTTCCCCGACGGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACTAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAaga                                                                                                                                                                                               <  1:788787/250‑1 (MQ=255)
                                                       gCTGCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGTTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGaa                                                                                                                                                                                                 <  2:233158/248‑1 (MQ=255)
                                                        cTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACACGCTGGAAGAGGCCGGTGACGAATTCGCCGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAagag                                                                                                                                                                                              >  1:609377/1‑250 (MQ=255)
                                                           ccccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGc                                                                                                                                                                                             <  2:121096/248‑1 (MQ=255)
                                                           ccccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCAt                                                                                                                                                                                           >  1:1000111/1‑250 (MQ=255)
                                                             ccGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATgg                                                                                                                                                                                         <  1:235007/250‑1 (MQ=255)
                                                              cGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGa                                                                                                                                                                                        <  1:1317441/250‑1 (MQ=255)
                                                              cGACTGCGCACATCTGGTCCCTGAAATCGCTCCCTTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGa                                                                                                                                                                                        <  2:628055/250‑1 (MQ=255)
                                                                aCTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATc                                                                                                                                                                                      >  1:442847/1‑250 (MQ=255)
                                                                    cgcCCATCTGGTTCCTGAAATCGCTGCCGTCCCGTTTCGGTCTGCTTCTCGATATGCCGCTTCGCGATATCGAACGCGTTCTGTTCTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGa                                                                                                                                                                                  <  1:25071/250‑1 (MQ=255)
                                                                    cgcACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTg                                                                                                                                                                                    <  2:996148/248‑1 (MQ=255)
                                                                         aTCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGc                                                                                                                                                                                >  2:665891/1‑247 (MQ=255)
                                                                                 ccTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGa                                                                                                                                                                      <  1:1426468/249‑1 (MQ=255)
                                                                                  atggaaTAGCTGACGTACGGTAGCGGTCGTCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTAGTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAAAGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATGTGGAGCAAGAGTGCGAAc                                                                                                                                                                    <  2:429127/246‑1 (MQ=255)
                                                                                     aaaTCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAg                                                                                                                                                                  <  1:761296/249‑1 (MQ=255)
                                                                                      aaTCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCt                                                                                                                                                                <  1:1035285/250‑1 (MQ=255)
                                                                                       agcgatGCCGTTCCTTAGCGGTCTGCTGCTCGATAGGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAAGCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTg                                                                                                                                                               <  2:133113/245‑1 (MQ=255)
                                                                                          gCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGt                                                                                                                                                            <  2:526254/250‑1 (MQ=255)
                                                                                              ccGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTg                                                                                                                                                           >  1:120826/1‑247 (MQ=255)
                                                                                               cGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAaga                                                                                                                                                       <  2:448633/250‑1 (MQ=255)
                                                                                                gTCCCGTATCTGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAagag                                                                                                                                                      <  1:1354242/250‑1 (MQ=255)
                                                                                                gTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAagag                                                                                                                                                      >  1:481865/1‑250 (MQ=255)
                                                                                                gTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAagag                                                                                                                                                      <  2:613700/250‑1 (MQ=255)
                                                                                                 tCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGCTCTGTAGCAAGAGTGCGAACCGCTGCGTGAAGGGc                                                                                                                                                     >  2:17205/1‑250 (MQ=255)
                                                                                                 tCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGACCAGCTGCGTGAAGAGc                                                                                                                                                     >  2:881672/1‑250 (MQ=255)
                                                                                                 tCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGc                                                                                                                                                     <  1:1313584/250‑1 (MQ=255)
                                                                                                   ccGTATCGGTCTGCGGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAAGCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGTCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTg                                                                                                                                                   <  2:1059690/250‑1 (MQ=255)
                                                                                                     gTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGaa                                                                                                                                                 >  2:273300/1‑250 (MQ=255)
                                                                                                          ggTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGACCAGCTGCGTGAAGAGCTGAACGaaa                                                                                                                                            >  2:1336129/1‑250 (MQ=255)
                                                                                                              tgctgcTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGATCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGCCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCaa                                                                                                                                        >  1:258721/1‑250 (MQ=255)
                                                                                                                ctgctCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATATGGAGCAAGAGGGCGAACAGCTGCGAGAAGAGCTGAACGAACCAAACt                                                                                                                                      >  2:933891/1‑250 (MQ=255)
                                                                                                                ctgctCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGAGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCaa                                                                                                                                        <  2:1071155/248‑1 (MQ=255)
                                                                                                                 tgctCGATATGCCGCTGCGCGATATCGAACGCGCACTGTCCCTTGAATCCTATGTGGTTATCGACGGTGGTCTGACCAACCTGGAACGTCTGCAGATCCTGACTGAAGAGCAGTATCTGGACACGCCGGAAGAGGGCGGCGACGAATTCGACGCGTAGATGGGGGCGGACGCAGTCCAGGCTCTGCTGAAGAGCAGGGATCCGGAGCACGAGTGCGAGCAGCTGAGAGACGAGCCGAACTAAGCCAACTc                                                                                                                                     >  2:1278890/1‑250 (MQ=255)
                                                                                                                   ctCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTcc                                                                                                                                    <  2:12509/249‑1 (MQ=255)
                                                                                                                   ctCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCg                                                                                                                                   <  1:1167206/250‑1 (MQ=255)
                                                                                                                      gATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGaaa                                                                                                                                <  2:1420023/250‑1 (MQ=255)
                                                                                                                      gATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGa                                                                                                                                  <  1:815176/248‑1 (MQ=255)
                                                                                                                      gATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGa                                                                                                                                  <  1:112824/248‑1 (MQ=255)
                                                                                                                             cGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCg                                                                                                                         >  2:306295/1‑250 (MQ=255)
                                                                                                                                   gcgATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTaaaaa                                                                                                                   <  2:594300/250‑1 (MQ=255)
                                                                                                                                      atatCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCt                                                                                                                >  1:123638/1‑250 (MQ=255)
                                                                                                                                        atCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCGGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGa                                                                                                              >  1:651116/1‑250 (MQ=255)
                                                                                                                                         tCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGAc                                                                                                             <  1:898888/250‑1 (MQ=255)
                                                                                                                                            aaCGCTTACTGGACTGTGAAGCCTAAGTGGGTATCGAAGGCGGTAAGACCAACCTGGAACGTCAGCAGATCCTGACTGGAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCAGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGGCCaa                                                                                                          <  2:1066158/250‑1 (MQ=255)
                                                                                                                                             aCGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGACGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCGGACAAAg                                                                                                         >  1:830155/1‑250 (MQ=255)
                                                                                                                                              cccgTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGGCCGACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTAGCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAGCCAAGCGTAAAAAGCTGACCAAGc                                                                                                        <  2:317994/248‑1 (MQ=255)
                                                                                                                                                    ctgtactTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCa                                                                                                  <  1:74330/250‑1 (MQ=255)
                                                                                                                                                     tgtactTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGACCGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGCGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGATGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACCCCGAAACCACGCGTAAAAAGCTGACCAAGCGTACCa                                                                                                  >  2:212714/1‑249 (MQ=255)
                                                                                                                                                     tgtactTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGACCGTCAGCAGATCCAGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGACTTCGACGCGAAGATGGGGGCGGAAGCAATCAAGGCTCTGCTGACGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGCGAAGCGCTGAACGAACTCAACTCAGAAACCAAGCGTACAAAGCTGACCAAGccact                                                                                                    >  2:1093548/1‑243 (MQ=255)
                                                                                                                                                     tgtactTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCa                                                                                                  >  1:548518/1‑249 (MQ=255)
                                                                                                                                                         ctTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAActg                                                                                             <  1:1156244/250‑1 (MQ=255)
                                                                                                                                                         ctTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAAct                                                                                              <  1:659258/249‑1 (MQ=255)
                                                                                                                                                           ttGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCACActgct                                                                                           >  2:1049196/1‑250 (MQ=255)
                                                                                                                                                              aaTCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGa                                                                                        <  1:713960/250‑1 (MQ=255)
                                                                                                                                                               aTCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTgg                                                                                         >  1:535334/1‑248 (MQ=255)
                                                                                                                                                                  cTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGc                                                                                     <  2:170023/249‑1 (MQ=255)
                                                                                                                                                                    aTGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGcgtt                                                                                  >  2:290773/1‑250 (MQ=255)
                                                                                                                                                                        ggTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACACAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGcgttcgtt                                                                              >  2:543765/1‑250 (MQ=255)
                                                                                                                                                                            aTCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAg                                                                           <  1:455228/249‑1 (MQ=255)
                                                                                                                                                                             tCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGt                                                                          <  2:886360/249‑1 (MQ=255)
                                                                                                                                                                              cGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGGTCGTTCAGTCt                                                                        <  2:945821/250‑1 (MQ=255)
                                                                                                                                                                               gAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTg                                                                       <  1:1109205/250‑1 (MQ=255)
                                                                                                                                                                                aaGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTgg                                                                      >  1:28318/1‑250 (MQ=255)
                                                                                                                                                                                aaGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTgg                                                                      >  2:504017/1‑250 (MQ=255)
                                                                                                                                                                                   gCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTaa                                                                   <  1:87188/250‑1 (MQ=255)
                                                                                                                                                                                    cGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAAc                                                                  <  1:578184/250‑1 (MQ=255)
                                                                                                                                                                                        aTGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTAGTTCAGTCTGGTAACAAAc                                                              >  1:375489/1‑250 (MQ=255)
                                                                                                                                                                                           aCCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCa                                                            <  1:936322/249‑1 (MQ=255)
                                                                                                                                                                                             cAACCTGGAACGACAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCagag                                                         <  1:7914/250‑1 (MQ=255)
                                                                                                                                                                                                ccTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGACGCGTTCGTTCAGTCTGTGAACAAACCAGAGTg                                                       >  2:776999/1‑249 (MQ=255)
                                                                                                                                                                                                    gAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatga                                                  <  1:1105168/250‑1 (MQ=255)
                                                                                                                                                                                                    gAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatga                                                  <  1:37943/250‑1 (MQ=255)
                                                                                                                                                                                                    gAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGgatg                                                   <  2:327328/249‑1 (MQ=255)
                                                                                                                                                                                                         tCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTg                                             <  1:413576/250‑1 (MQ=255)
                                                                                                                                                                                                           agcacATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGAAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGAc                                           <  1:719766/250‑1 (MQ=255)
                                                                                                                                                                                                             cagATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCg                                         <  2:348787/250‑1 (MQ=255)
                                                                                                                                                                                                               gATCATGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGACCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAGCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCATTCTGGTAACAAACCAGAGTGGATGATCCTGTCCGtc                                       >  2:115153/1‑249 (MQ=255)
                                                                                                                                                                                                                  ccTGACTGAACAGCAGTATCTGGCCGCGCTGGAAGAGGTCGGTGACGAATTCGCCGCGAAGATGGGGGCGGCAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGCGCGCACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAACGCTGACCAAGCGTATCACACTGCTGGAAGCGTTCGTTCACGCTGGTAACAAACCAGAGTGGCGGATCCTGACCGTTCtc                                    >  2:1314843/1‑249 (MQ=255)
                                                                                                                                                                                                                     gactgaAGAGCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCg                                 >  2:825481/1‑250 (MQ=255)
                                                                                                                                                                                                                             agCAGTATCTGGACGCGCTGGAAGAGGTCGGTGACGAAGTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgcc                         <  1:1046357/250‑1 (MQ=255)
                                                                                                                                                                                                                                 gTATCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccgc                       >  1:271789/1‑248 (MQ=255)
                                                                                                                                                                                                                                   aTCTGGACGCGCTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCCCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccgc                       >  1:693137/1‑246 (MQ=255)
                                                                                                                                                                                                                                            cgcTGGAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTcc          >  2:406850/1‑250 (MQ=255)
                                                                                                                                                                                                                                                ggAAGAGGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTg      <  2:1099228/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaGAGATCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGt    <  1:1376469/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    gagGTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTc  >  1:1049741/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
CGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATATGCCGCTGCGCGATATCGAACGCGTACTGTACTTTGAATCCTATGTGGTTATCGAAGGCGGTATGACCAACCTGGAACGTCAGCAGATCCTGACTGAAGAGCAGTATCTGGACGCGCTGGAAGAGTTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTC  >  NZ_CP009273/4175544‑4176037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑