Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	353,601	0	C	A	41.7%	12.7 / 13.8	37	L267F (TTG→TTT)	cynR	transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base C (15/6);  new base A (2/13);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 7.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCATC  >  NZ_CP009273/353353‑353840                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   cAAAAATGCCTTCGCCGCGTCGTTCTGCCAGCTTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCTTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTTTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGcaaca                                                                                                                                                                                                                                                   <  1:2132075/250‑1 (MQ=255)     aaTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCCGCGGCCACCGTGATTCATTT‑CCGCCA‑ACAACCGCCCCCTTATCCAACGCCATGTG‑GAAAAAAG‑CCCTTCCGGCGGGC‑GGCTGTCCCCTCTTTTT‑CTCCCGCCACCAAA‑c                                                                                                                                                                                                                                              >  2:526750/1‑250 (MQ=17)     aaTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑TCGCCA‑ACAACCGCGCATTTATCCAACGCCATTTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTTCCCGCTATTTTT‑CCGCCGCCACAAAA‑c                                                                                                                                                                                                                                              >  1:1263089/1‑250 (MQ=255)       tGCCTTCGCAGCGGCTGTCTGCCATCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑ccg                                                                                                                                                                                                                                            <  2:688117/250‑1 (MQ=255)         ccTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑ccccc                                                                                                                                                                                                                                          >  1:689155/1‑250 (MQ=255)           ttCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCC‑GTCTGCCCGCTATTTTT‑CCGCCGCAACAAAA‑ccgccgg                                                                                                                                                                                                                                        >  1:1365637/1‑249 (MQ=255)             cGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCCACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCC‑GTTTGGCCGCTATTTTT‑TCGCCCCCAACAAA‑CCCCCGGtc                                                                                                                                                                                                                                      >  2:599458/1‑250 (MQ=17)                         cTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGGCGTTCTCTCCCGTAGTg                                                                                                                                                                                                                          >  1:73701/1‑250 (MQ=255)                              aGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCC‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                                                                                                                     >  1:413634/1‑250 (MQ=255)                              aGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑TCCCCGCAACAAAA‑CCGCCGTTCTCTCCCGTCGTGGCggg                                                                                                                                                                                                                     >  2:279121/1‑250 (MQ=255)                              aGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                                                                                                                     >  1:720903/1‑250 (MQ=255)                                cTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTAACGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAAAG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAAAAAAA‑CAGCCGTTCGCTCCAGTAGTGGCgggcg                                                                                                                                                                                                                   >  2:2080895/1‑248 (MQ=255)                                      tttATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGCTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCCTTT‑ACGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑CTCCGCCAGCTATTTTT‑CCGCCGCCACAAAA‑CCGCCGGTGTTTCCCGCACTGGCGGAGCacagc                                                                                                                                                                                                              >  2:1697741/1‑245 (MQ=17)                                        tATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCCGCTATTTTT‑ACGCCGCAACAAAA‑CCGACGTTCTCTCCCGTAGTGGCggggggtgtggg                                                                                                                                                                                                            >  2:767811/1‑241 (MQ=255)                                             cGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGCAC‑A‑Gaaaaatac                                                                                                                                                                                                    >  1:757532/1‑245 (MQ=255)                                                aTGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGAGGAA‑GAAAAAGCtt                                                                                                                                                                                                   >  1:2238525/1‑249 (MQ=255)                                                                   gccggcgagggTAGAGCTCTGACGTGGGAGGCATGGTAGGCGTGGCGATGCAGGAAACCCCGCCCGCAGACTTGTGCCGGCGCCTCCCGTGATCCATCC‑CCCCCA‑ACAACCGCGCTTTTCCCCAACGCCATGTG‑AAAAAATC‑CCTGCGCGGCGGCT‑GTCTGCTAGTTATTTTC‑CCGCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTgg                                                                                                                                                                              <  2:1302860/240‑1 (MQ=255)                                                                       gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCCAG‑AGGAAAAGGCTTTAAACCCGCCCCTTTTTGTGGCat                                                                                                                                                                           >  1:1168499/1‑249 (MQ=18)                                                                                                 gCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGTGCAAG‑C‑GACATAACATTTATATCATCCCGTTCTGTGTCTAGCCATGCTCGTAACAAAGTGGGAAgg                                                                                                                                                 >  1:894658/1‑249 (MQ=18)                                                                                                        acgCGGAGCCCATCAGACACTCCCCCCCACAGCCTTTTGTCAGGCCCCACCGT‑ATTTATTC‑CCCCCACACACCCGCCCTTTAACCCACCGCCTTTTG‑AAAAACGG‑CTTGCGCGGGGGCT‑GTCTGCCGGTTTTTTCC‑CCCCCACAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcg                                                                                                                                         <  2:1027674/248‑1 (MQ=255)                                                                                                              aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAG‑AGGAAAAAACTTTAA‑ACCGCCCAGTTTTTGGGGAATCGACGCTGGGGACAAAGTGGAGAAGGGAGTggggcg                                                                                                                                      >  1:1725180/1‑247 (MQ=255)                                                                                                                   agccggCAATCCCCCCCGCCGGCCGGTGTCGCGGGCTACCGTGATTTCTTC‑CCCCCA‑AACCCCCCCTCTTTTTCCCACCCCCTGGG‑CAAAAAAG‑CCCTCGGGGGGGGTGGCCTGCATGTTTTTTTC‑CCCCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGc                                                                                                                               <  2:723097/246‑1 (MQ=255)                                                                                                                                        tcgggggcgggcgcCTACCGTGATTAGTTTCCCCCCA‑ACACCCGC‑CATTTTACCAGCGCC‑TGTGTAAAAAATGCCCTGCGGGGGGGTT‑G‑CTACGCGGTATTGTA‑CCCCCCCAATAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg                                                                                                          <  2:1918682/240‑1 (MQ=255)                                                                                                                                                                   attcccccaa‑acaccCCCGCATTTACCCACCGCCATGTA‑AAAAAAGC‑CCTCCGCGGCGGCT‑GTCTGCTAGTTTTTTTC‑CCGCCCAAAAAAAT‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGt                                                                               <  1:953503/241‑1 (MQ=255)                                                                                                                                                                                      gcATTACTCCAACGCCATGTG‑CAAAAAGG‑CCTGCGGGGCGCCT‑TGCTGCCATTTTTTTTC‑CCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCag                                                             <  1:268116/250‑1 (MQ=255)                                                                                                                                                                                                    cctggtg‑CAAAACGG‑CCTCGGGGGCGGTT‑GTCGCCCAGTTTTTTTC‑CCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTg                                               <  1:1377674/246‑1 (MQ=255)                                                                                                                                                                                                     ccatgaaaaaaaaCG‑CCTTGGCGGCGGCT‑GTCTGCCAGTTTTTTCC‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTg                                               <  1:105948/242‑1 (MQ=255)                                                                                                                                                                                                         gtaaaaaaaTC‑CCTGCGCGGGGGTT‑TTCGCCCAGCTATTTTC‑CCGCCGCAACAAAG‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGct                                             <  1:8317/244‑1 (MQ=255)                                                                                                                                                                                                            aaaaaaTC‑CCTCCGCGGCGG‑T‑GTTCGCCCACTTTTTTTCCCGCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCtg                                        <  1:1136669/249‑1 (MQ=255)                                                                                                                                                                                                             aaaaaTG‑CTCTCGGGGGGTGT‑GTCCTCCAGTTTTTTTT‑CCCCCGCAACAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCtg                                        <  2:1454134/250‑1 (MQ=255)                                                                                                                                                                                                             aaaaaTG‑CCTCCGGGGGGGTG‑GTCTGCCATCTTTTTTC‑CCGCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGctctcttg                                       <  1:1663465/250‑3 (MQ=255)                                                                                                                                                                                                                               ggcgtgtcgtccAGCTTTTTTC‑CCCCCCCAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCgcgc                        <  2:1767679/240‑1 (MQ=255)                                                                                                                                                                                                                                 ttgcgcggCCCGCTATTTCC‑CCGCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGGAAATTCCGCGCTGa                     <  1:523251/243‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCATCTATTTCG‑CCGCTGAAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAg                 <  1:119108/250‑1 (MQ=255)                                                                                                                                                                                                                                         ccATCTTTTTTC‑CCCCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTGTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGAcc             <  1:2108190/250‑1 (MQ=255)                                                                                                                                                                                                                                           agatagtttg‑CCGCAGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAg           <  1:254161/244‑1 (MQ=255)                                                                                                                                                                                                                                                ttttc‑CGGCCGCAACAAAG‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGtttt       <  1:390475/249‑1 (MQ=255)                                                                                                                                                                                                                                                     cccgcccCAAAAAAG‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTtcatc  <  1:997159/249‑1 (MQ=255)                                                                                                                                                                                                                                                      cccccccaaaaaAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTtcatc  <  1:2265477/243‑1 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   CAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTT‑CCGCCA‑ACAACCGCGCATTTATCCAACGCCATGTG‑CAAAAATG‑CCTTCGCGGCGGCT‑GTCTGCCAGCTATTTTT‑CCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑A‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGACCACCTGTGGATGTAGCCCCGCTTTCTCGCAGTAGTGGTCAATTTGCTCTCTGGTGGCAAATTCCGCGCTGAGCAGGACCAGTTTTTCATC  >  NZ_CP009273/353353‑353840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑