Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,036,3900AG40.7% 75.4 / 13.6 55N206D (AAT→GAT) srkAstress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base A (16/16);  new base G (22/0);  total (39/16)
Fisher's exact test for biased strand distribution p-value = 3.63e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCT  >  NZ_CP009273/4036146‑4036632
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
ggTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATAtt                                                                                                                                                                                                                                                 >  1:507123/1‑250 (MQ=255)
        gTTATTTGGGGCGTATGCACCAGTCGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGAGGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGgcg                                                                                                                                                                                                                                         <  1:63376/250‑1 (MQ=255)
        gTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTTgcg                                                                                                                                                                                                                                         >  1:103565/1‑250 (MQ=255)
           aTTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAAATTTCACCGTTCTTCGGCGACATGGAGACTGCCCCGCCGGGGAGATTCTATGgcgc                                                                                                                                                                                                                                        >  2:697087/1‑248 (MQ=255)
                      aTGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACCTGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAtgt                                                                                                                                                                                                                           >  1:871980/1‑250 (MQ=255)
                           ccAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTTTCCGGCTCCATGGAGACAGCCACCCCGGGGAGATTTTCCTGCGCGGTGGGCCCAttattttt                                                                                                                                                                                                                      >  2:626853/1‑243 (MQ=255)
                                         aaCAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGTTCTGCGGGCACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGTTGGGCCAATGGTTGTTTGTTTGggtgattg                                                                                                                                                                                                        >  1:609679/1‑248 (MQ=255)
                                         aaCAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGGTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGGCCCATGTTTGTTGGTCTGGgtgatg                                                                                                                                                                                                         >  1:287895/1‑249 (MQ=255)
                                                        aTCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTCACCGTTTTTCGGCTACATGGAGACTGCCCCGCCGGGGGTATTCTCTGGCGCGATGGGCCAATGTTTGTTGCTCTGGATGATTCACGTAATGGTCCAGc                                                                                                                                                                                         >  2:935815/1‑250 (MQ=255)
                                                        aTCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTTCGGCTACATGGAGACTGCCCCCCCCGGGATATTCTTTGGCGCGATGGTTCCATGTTTGTTGATCTGGGTGATGCACGTAATCGTCCaac                                                                                                                                                                                         >  1:728132/1‑248 (MQ=255)
                                                             ccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCCTTCTTCGGCCACATGGAGAACTCCCCCCCGGGGAAATTTTCTTGCGCGGTGGGCCACTGGTTGTTTGTCTGGATGATTCACGTAATGGTCCCGCCGTTc                                                                                                                                                                                    >  2:642651/1‑250 (MQ=255)
                                                                  cATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTGCAGCCGTTCAGGAt                                                                                                                                                                               >  1:120792/1‑250 (MQ=255)
                                                                                       cATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCCGGGATATTCTCTTGCGCGGTGGTCCAATGTTTGTTGATCTGGGTGATGCACGTAAAGGTCCAACCGTTCAGGGTTTGTGGGTGTTGCTCAATGGc                                                                                                                                                          >  1:98143/1‑250 (MQ=255)
                                                                                          tGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCCACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTACTGGTCCAGCCGGTCCGGGTTTGTGGATGtttgtttatggcgg                                                                                                                                                        >  2:703734/1‑237 (MQ=255)
                                                                                                acgcacgcTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGGGGCTACATGGAGACTGCCACGACGGGAATATTTTCTGTCGCGCTGGTTCAATGTTTGTTTATATGGATGATGCACGTAAGGGTCCAGCCGTTGAGGATTTGTGGTTGTTGCTTAATGGGGATTAA‑g                                                                                                                                                  >  2:708638/1‑248 (MQ=255)
                                                                                                aCGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGAAATTCTCTGGCGCGATGGTCCAATGTTTGTTGATTTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑Gcc                                                                                                                                                >  1:304424/1‑250 (MQ=255)
                                                                                                  gCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACCGCCACGCCGGGGATATTCTCTGGCGCGGTGGTCCCATGTTTGTTGGTCTGGGTGATGGCCGTAATGGTCCAGCCGTTCAGGGTTTTTGGGTGTTGCTCCATGGGGATGAA‑Gcc                                                                                                                                                >  2:584897/1‑248 (MQ=255)
                                                                                                    aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGGTCTGCGGCCCCATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGGCCAATGGTTGTTGATATGGATGATGCCCGTAATGGTCCAGCCGTTCCGGATTTGTGGGTGTTGCTCAATGTCGATAAA‑GCCGagc                                                                                                                                            >  2:476259/1‑250 (MQ=255)
                                                                                                        tGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCg                                                                                                                                        >  1:835108/1‑250 (MQ=255)
                                                                                                                 aCGTTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGAAAGATGGGCTGTTTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACt                                                                                                                               <  2:99009/250‑1 (MQ=255)
                                                                                                                       cacTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGTTGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGaaa                                                                                                                          <  1:867626/249‑1 (MQ=255)
                                                                                                                               ccTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTTCATGGAGAACGCCCCGCCGGGGATATTTTCTGGCGCGCTGGTCCAATGGTTGTTGTTCTGGATGATGGCCGTAATGGTCCAGCCGGTCCGGATTTGTGGGTGGTGGTCAATGGCGATAAA‑ACCGAGCAGCGGGTGCCAACGGGAACTcttattt                                                                                                                 >  2:876319/1‑249 (MQ=255)
                                                                                                                                         tGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAGTTCACCCTTCTTCGGCCACATGGAGACTGCCACCCCGGGGATATTTTCTGGCGCGGTGGGTCAATGTTTTTTGGTTCGGGTGATGCACGTAATGGTCCAGCCGGTCAGGATTTTTGGGTGTTGTTCAATGGGGGATAA‑GGCGAGGAGCGGGTGGCACAGGAAACTATTTTTTAAACTTATg                                                                                                        >  2:420520/1‑249 (MQ=255)
                                                                                                                                           aaaGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTTAAGCTTATg                                                                                                        >  1:239924/1‑247 (MQ=255)
                                                                                                                                               cGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑tt                                                                                                >  1:709622/1‑250 (MQ=255)
                                                                                                                                                ggCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGGCTTCACCGTTATGCGGCCACACAGGGACTGCCACGCCGGGCATATTCCTTGGCCGGCTGGGCCCCTGCTTTTTTGTTTGGGTGAAGCACCTTATGGTCCAGCCGTTTCGGGTTTTGGGGTGTTTGTCAATGGCGCTAAAGGCGGGGAAGCGGTGGAAACGGAAAATTATATTTGAA‑CTTATTGAGAA‑ttt                                                                                               >  2:297472/1‑250 (MQ=255)
                                                                                                                                                ggCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑ttt                                                                                               <  1:935815/250‑1 (MQ=255)
                                                                                                                                                  cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGGTGCAACTGGGAAATATTATTGAAACTTATGAAGAA‑TTTAg                                                                                             >  1:433078/1‑250 (MQ=255)
                                                                                                                                                  cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACCCCGGGGATATTCTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGAGTTGTGGGTGTTGCTCAATGGCGGTAAA‑GCCGAGCAGCGGATGCAACTGGGAACTATTATTTAAGCTTATGAAGAA‑TTTAg                                                                                             >  2:396832/1‑250 (MQ=255)
                                                                                                                                                       cTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGGTGGGTCAATGTTTGTTGATCTGGGTGATGCACGGAATGGTCCAGCCGGTCAGGGGTTGTGGATGGTGGTCAATGGCGATTAA‑GCCGGGCAGCGGATGCAACCGGGGAATATTTTTGGAGATTTTGATGAAGTTTAggggg                                                                                         >  2:66782/1‑246 (MQ=255)
                                                                                                                                                                aCAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCg                                                                               <  1:387997/250‑1 (MQ=255)
                                                                                                                                                                    aTGGGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGa                                                                           <  1:402511/250‑1 (MQ=255)
                                                                                                                                                                              tGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTTTGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACt                                                                  >  1:11832/1‑249 (MQ=255)
                                                                                                                                                                                  gccgTTACAGCACACTGGCGGGAAGATTTTTCCGTTTTTCGGCTACATGGGGACCGCCACGCCGGGGATATTTTCTTGGGCGGTGGTCCTATGTTTGTTGATTTGGGTGATGCAAGGAATTGTTCCGCCGGTCAGGCAATTTGGATGTTGGTCAAATGCGGTAAA‑AACGAGCAGGGGAGGGAAATGGGAAATATTATTTAAGCTTATGGAAAA‑TTTTTGGGGGTCGACACCGCGTGAATCGGGCTGGttt                                                             >  2:789344/1‑249 (MQ=255)
                                                                                                                                                                                        aCAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCt                                                        <  2:837653/249‑1 (MQ=255)
                                                                                                                                                                                              ctcTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCGTTAcgcg                                                 <  2:489354/248‑1 (MQ=255)
                                                                                                                                                                                                cTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACgcgc                                                >  2:677097/1‑249 (MQ=255)
                                                                                                                                                                                                 tGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGT‑CAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCAt                                             >  1:771091/1‑250 (MQ=255)
                                                                                                                                                                                                    cGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGc                                           >  1:135391/1‑250 (MQ=255)
                                                                                                                                                                                                     gggAAGATTGCACCGTCCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCg                                          <  2:791705/250‑1 (MQ=255)
                                                                                                                                                                                                      ggAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTCGATCTGGATGATGCACGCAATGTTCCAGCCGTTCAGGTTTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGCTGCAACTGGAAACTATTATTGAAACTTATGAAGAA‑TTTAGCGAGTTCTACACCGATTAAATCAGACTTATTGCAACTTTACGCGCCAGGCGt                                         >  2:759186/1‑250 (MQ=255)
                                                                                                                                                                                                          gATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg                                     >  1:231665/1‑250 (MQ=255)
                                                                                                                                                                                                            tttCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGtt                                   >  1:250020/1‑250 (MQ=255)
                                                                                                                                                                                                             ttCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGtt                                   >  1:389214/1‑249 (MQ=255)
                                                                                                                                                                                                                         gggatACATGGATCCTGCCCCGCGGGAAATTTTCTCTGGCGCAAGGTTCCAATGTTTGTGGATCTGGAGGAGGCACGTAAGGGTCCAGCCGTTCAGGATTTGTGGATTTTGCTCAAGGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGTTTATAAAGAT‑TTTAGCGAGTTCGACCCCGCTAAAATCGGACTGTTTGACCCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCt                      <  2:533967/246‑1 (MQ=255)
                                                                                                                                                                                                                           gCTACATGGATACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTgg                    <  2:652073/250‑1 (MQ=255)
                                                                                                                                                                                                                            cTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGc                   >  1:165208/1‑250 (MQ=255)
                                                                                                                                                                                                                               cATGGGGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGAGTTGGGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTa                 <  2:695760/249‑1 (MQ=255)
                                                                                                                                                                                                                               cATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGGA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCt                  <  2:103565/248‑1 (MQ=255)
                                                                                                                                                                                                                                aTGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAAt               <  1:584897/250‑1 (MQ=255)
                                                                                                                                                                                                                                    agaCTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgcgg           >  1:788110/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gaCTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgcggc          <  2:599048/250‑1 (MQ=255)
                                                                                                                                                                                                                                             cGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCt  <  2:710297/250‑1 (MQ=255)
                                                                                                                                                                                                                                             cGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCt  >  1:296103/1‑250 (MQ=255)
                                                                                                                                                                                                                                              gCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCt  >  1:471719/1‑249 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
GGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAA‑GCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAA‑TTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCT  >  NZ_CP009273/4036146‑4036632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑