| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,392,087 | 0 | T | C | 35.1% | 127.8 / 20.0 | 75 | I265V (ATT→GTT) | uspE | universal stress protein UspE |
| Reads supporting (aligned to +/- strand): ref base T (39/9); new base C (0/26); total (39/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAG > NZ_CP009273/1391847‑1392333 | ggATTTTATTTCCTTGGCGGAGAAGAGCAGTAATTAAAAAATAGGCCCGATAACTC‑GTGCCTTGT‑CAG‑TTATTGAAGATTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTg < 2:405101/250‑1 (MQ=255)ggAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCt < 2:323657/249‑1 (MQ=255) gAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTg > 2:580466/1‑249 (MQ=255) cggcgAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCTCCGCCTGTAAGTGCTCCGCCAAAt > 1:660931/1‑250 (MQ=255) gcgAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAAt > 1:462763/1‑248 (MQ=255) cgAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAg > 1:55189/1‑250 (MQ=255) cAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCAc > 1:499474/1‑250 (MQ=255) aagaaaAAAAGGGGCCCTATAACT‑‑GGGCCTTTTATAG‑TTTTTGAGTGTTTTTTAATCTTTTTCTTCCTCACCGTTTCCAGGGGGTG‑TTTGTTTCTGGTCAGTTTA‑AAGGCCAAGGAGGGCGGAGCAAAGATGACCACTCCCCTTTCCCCCCGTGTTGCCGAGGAA‑TGCTCCTAAAATCCCGGT‑GCGCCCTACCGCGCCCAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACctct < 2:643388/250‑1 (MQ=255) agtaaAAAATAGGCCCGATACCTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGTTG‑TCTGATACTGGTCAGGTTT‑AATGCCCAGCAGGTCGCAGCGAAGATGATAAATCCCCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATCCCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt < 1:328214/250‑1 (MQ=255) aaaaaTAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTgg > 2:547824/1‑250 (MQ=255) aaaaaTAAGCCCAGAAACTCGGGGCTTTTT‑CAG‑TTATGGAAGTGTTTTTAATCTTTTTTTTCGTCACCCA‑TTCCAAGGGTGTTCTGATCCTGGTCAGTTTT‑AAGGCCCAGCGGGCCGCAGCGAAGATAATAAACCCCCTGTCCCGCCGTTTTCCCGAGAAA‑TGCTGCTAAAATCCCGGT‑GCGTCCTCCCGTGCCCAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTg < 2:60724/250‑1 (MQ=255) aaaaTAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTgg > 1:482901/1‑249 (MQ=255) aaaaTAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTgg > 1:287937/1‑249 (MQ=255) gcccgtaaacc‑GGGCCCTGT‑CTGATTATAGAGGAGTCGTAAATCTTCTTCTCCGCCACCACGTACATGGGTTG‑TAGGAT‑GTTGTCCGGTTTAAAGGACCAGCAGGCGGAGGAGAAGATGAGCATCCCCCGGTCCCGCCGTTTGGCCGGGGAA‑TGCTGCTAAAAT‑CCGGTGGCTTCTAACCGCCCCAAAAC‑CCACATCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtt < 2:857707/240‑1 (MQ=255) aTAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTc > 2:431377/1‑249 (MQ=255) tGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGt > 1:530446/1‑250 (MQ=255) ttAGTGAAGTGTCGTAAATCGTTTGCGTCGTCACCCAGTTCAGGGGGTG‑TCTGATACTGGTGGGGTTT‑AATGCCCAGCGGGTGGAAGCGAAGATGATCAACCCCCGGTTCCGCCGTGTTGCCGAGAAA‑TGCTGCGAAAATCCCGGT‑CCGCCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCa < 2:933675/249‑1 (MQ=255) tGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAAt > 1:207993/1‑250 (MQ=255) gAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTACTg > 2:744284/1‑250 (MQ=255) agTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCg > 1:69339/1‑249 (MQ=255) tggttttctccGTCATCCAGTTAAAGGGGTG‑TTGAATTCGGGCCAGGTTA‑AAGGACCAGCGGGCCGCGGCGAAGATGATAAACCCCCTGTCCCGCTGTGTTGCCGAGGAA‑GGCTGCTGAAATACCGGT‑GCGTCCTACCGGGCCCAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc < 2:275743/245‑1 (MQ=255) aGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGc > 2:466186/1‑248 (MQ=255) gTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCGGAAATCCCGGT‑GCGTCCTACCGTGCCCAGAA‑CCAAAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAg < 1:322015/250‑1 (MQ=255) tCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGc > 1:665798/1‑249 (MQ=255) ggttttgtGATAGTGGTCAGGTGA‑AATCACCAGAAGGTCGCGGGGAAGATGATCAACCTCCTGTCCCGCCGTGTGGCCGAGGAA‑TGCTGCTAAAATACCGTG‑GCGTCCTACCGTGCCCAAAA‑CCACATTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGt < 2:329962/243‑1 (MQ=255) gtg‑tCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTg > 2:333127/1‑250 (MQ=255) tCTGATACTGGTCAGTTTT‑AAGGACCGGCGGGCCGCGGCGAAGATGACCAACCCCCTGTCCCGCCGTGTTCCCGAGGAA‑TGCTCCTAAAATCCCGGT‑GCGCCCCACCGCCCCCAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGcc < 2:92741/249‑1 (MQ=255) gATACGGTTCAGGTTT‑AAGGCCAAGCAGGCCGCAGAGAAAATGACCAATCCCCGGTCCCGCCTTGTGGCCGAGGAA‑TGCTGCAAAAATCCCGGT‑CCGCCCTCCCGTCCCCAGAA‑CCCCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCAc < 2:275838/249‑1 (MQ=255) aTACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGAGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTACTGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAATTGTTGCCCACg > 2:404037/1‑249 (MQ=255) aCTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAAt > 2:90860/1‑250 (MQ=255) ttGGTCAGGTTT‑AATGCCCAGCAGGTCGCAGCGAAGATGATCAATCCCCTTTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATCCCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATg < 1:342176/249‑1 (MQ=255) tttt‑AACGCCCAGCAGGCCGCGGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTAAAATCCCGGT‑GCGTCCTACCGTGCCCAGAA‑CCCCAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGtt < 1:178622/249‑1 (MQ=255) gTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGt > 1:691395/1‑249 (MQ=255) aaTGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATATCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCCTCGTtata > 2:280200/1‑249 (MQ=255) tgcccAGCAGGTCGCAGCGAAGATGATCAATCCCCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGCCCTCCCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAAc < 1:663376/247‑1 (MQ=255) tGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAAc > 2:291419/1‑250 (MQ=255) aCCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGc > 2:99725/1‑250 (MQ=255) ccAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGACTGGCATCGTTATAAACGCt > 1:98476/1‑250 (MQ=255) gcagGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCgg > 1:190698/1‑250 (MQ=255) cagGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCggg > 2:230489/1‑250 (MQ=255) gTCGCAGCGAAGATGATCAACCACCTGTTCCGCCTTGTTGCCGAGGAA‑TGCTGCTAAAATCCCGGT‑GCGTCCTCCCGCGCCAAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCa < 1:541415/250‑1 (MQ=255) gAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCgg > 1:185159/1‑250 (MQ=255) gatCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTACGGCAGtt > 2:405774/1‑249 (MQ=255) ccactccccTGCTCCCCCGTTTTGCAGGGGAA‑TTCGTCTAAAATCCCGTG‑CCTTCCTACCGGCCCCAAAC‑CCACACACCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCaa < 2:791743/243‑1 (MQ=255) caatcaCCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAAt > 1:494456/1‑250 (MQ=255) cTGTCCCGCCGTGTTGCCGAGGAA‑TGCGGCAGAAATGCCGGC‑CGCTCCTACCGCGCCCAGAA‑CCCAAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTGATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGata < 2:605962/250‑1 (MQ=255) ttgcgaccgggtGCCGAAGAATTGCCGCAAAAATCCCGGT‑GCCCCCACCCGGGCCCAGAC‑CCACAACCACCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATAtt < 2:384015/239‑1 (MQ=255) tCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTaa > 1:842683/1‑250 (MQ=255) gtgtTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCg > 1:747707/1‑250 (MQ=255) tttGGCGAGAAT‑TGCCTCTAAAATCCCGGG‑GGGCCCTCCCGTGCCCAAAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGtt < 1:544792/249‑1 (MQ=255) gtTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCCCGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTACTTGGCGt > 2:725971/1‑249 (MQ=255) tGCCGAGAAA‑TTCTGCTAAAATCCCGGT‑GCGCCCTACCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTAc < 2:249199/250‑1 (MQ=255) gaattgctgcTAAAATCCCGGT‑GCGTCCTACCG‑GCCCAAAACCCCCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATa < 2:954575/246‑1 (MQ=255) tgctgcTGAAATCCCGGT‑GCTCCCTACCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAgcg < 1:206685/250‑1 (MQ=255) tgctgcTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAgcg > 1:513163/1‑250 (MQ=255) tgctgAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGcc > 1:484452/1‑249 (MQ=255) ctgAAATACCGTT‑CCGTCCTACCGTCCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCaa < 2:803735/249‑1 (MQ=255) tgAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAAc > 2:134855/1‑249 (MQ=255) aaTCCCGGT‑GCTTCCTCCCGTGCCCAGAC‑CCACATCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGa < 1:881099/250‑1 (MQ=255) aaTCCCGGT‑GCGTCCTACCGTCCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGa < 1:307895/250‑1 (MQ=255) tACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGAt > 1:646867/1‑249 (MQ=255) gT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACtt > 1:621874/1‑250 (MQ=255) t‑GCCTCCCACCGTGCCAAGAC‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTc < 2:475455/250‑1 (MQ=255) cGTCCTACCGTGCCCAGAA‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCg < 2:171633/249‑1 (MQ=255) cTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTa < 1:884337/248‑1 (MQ=255) ccGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATgg < 1:134855/248‑1 (MQ=255) tGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAATTCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCATTGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTCGAc > 1:350569/1‑250 (MQ=255) ccaaacccaccaCCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTg < 2:706059/244‑1 (MQ=255) catca‑CCACAACTCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCGCTTCTGGCATACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGt < 2:70036/246‑1 (MQ=255) ca‑CCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCt < 1:446294/249‑1 (MQ=255) cccgaaaTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTg < 1:604833/245‑1 (MQ=255) cccACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTg < 1:439549/249‑1 (MQ=255) acaACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCa < 1:466238/250‑1 (MQ=255) caATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAg > 1:704215/1‑250 (MQ=255) caATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAg > 1:174341/1‑250 (MQ=255) | GGAATTTATTTCCTTGGCGGCGAAGAGCAGTAAGTAAAAAATAGGCCCGATAACTC‑GGGCCTTGT‑CAG‑TTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTG‑TCTGATACTGGTCAGGTTT‑AATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAA‑TGCTGCTGAAATACCGGT‑GCGTCCTACCGTGCCCAGAA‑CCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTTATAAACGCTCGGGTCAAATTCCGGCAGTTCAATCGCGATATTAATTGGCGTTACCGGATAAGCGCCAACCAGATGAACTTCGGTATGGTTGACTTGTTCTGCCAG > NZ_CP009273/1391847‑1392333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |