Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,592,733	T→C	intergenic (‑390/+141)	BW25113_RS07915 ← / ← lsrK	ECSE_1600 family autotransporter/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,592,733	0	T	C	100.0%	360.5 / NA	93	intergenic (‑390/+141)	BW25113_RS07915/lsrK	ECSE_1600 family autotransporter/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/43);  total (50/43)

CTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAG  >  NZ_CP009273/1592486‑1592978                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATtctc                                                                                                                                                                                                                                                     >  2:876300/1‑250 (MQ=255)  tttCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTcc                                                                                                                                                                                                                                                    >  2:792219/1‑250 (MQ=255)     cGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCtt                                                                                                                                                                                                                                                 >  2:739259/1‑250 (MQ=255)     cGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAAAT‑TCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCtt                                                                                                                                                                                                                                                 <  2:458430/249‑1 (MQ=255)      gAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCtt                                                                                                                                                                                                                                                 >  1:518598/1‑249 (MQ=255)       aaTTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGa                                                                                                                                                                                                                                               <  1:175184/250‑1 (MQ=255)       aaTTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTCTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTg                                                                                                                                                                                                                                                >  1:809617/1‑249 (MQ=255)       aaTTGATAGATTAAAAATTGCATAGTTCTTATTTTAATTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGa                                                                                                                                                                                                                                               <  2:133466/250‑1 (MQ=255)               gATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGt                                                                                                                                                                                                                                        <  2:596361/249‑1 (MQ=255)                 ttAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACt                                                                                                                                                                                                                                     <  2:223794/250‑1 (MQ=255)                    aaaaTTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTgg                                                                                                                                                                                                                                   <  1:719286/249‑1 (MQ=255)                      aaTTGCATAGTTCTTCTTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGa                                                                                                                                                                                                                                >  2:310647/1‑250 (MQ=255)                             tAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAAc                                                                                                                                                                                                                          >  1:691866/1‑249 (MQ=255)                               gTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGAt                                                                                                                                                                                                                       <  2:759775/250‑1 (MQ=255)                               gTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGAt                                                                                                                                                                                                                       >  1:2111/1‑250 (MQ=255)                                ttCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATg                                                                                                                                                                                                                      <  1:430465/250‑1 (MQ=255)                                  cTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGaa                                                                                                                                                                                                                    >  1:887392/1‑250 (MQ=255)                                  cTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGaa                                                                                                                                                                                                                    >  2:573560/1‑250 (MQ=255)                                   ttattttattTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAAt                                                                                                                                                                                                                   >  2:601956/1‑250 (MQ=255)                                   ttattttattTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAAt                                                                                                                                                                                                                   >  1:90576/1‑250 (MQ=255)                                                atatGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCt                                                                                                                                                                                                      <  2:461396/250‑1 (MQ=255)                                                      aCTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAAc                                                                                                                                                                                                <  2:547928/250‑1 (MQ=255)                                                       cTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAAc                                                                                                                                                                                                >  1:960697/1‑249 (MQ=255)                                                        tATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAAc                                                                                                                                                                                                >  1:621714/1‑248 (MQ=255)                                                          tGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACTAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGCATCTGCAAGCTGGCTTTTAACAAGc                                                                                                                                                                                            <  2:425411/250‑1 (MQ=255)                                                            aattATTGTTGCTTAATAAGAAAACGGTTGCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCTAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCa                                                                                                                                                                                          <  2:731428/248‑1 (MQ=255)                                                             gTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAg                                                                                                                                                                                         <  1:127453/250‑1 (MQ=255)                                                                aTTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGctc                                                                                                                                                                                      >  2:605533/1‑250 (MQ=255)                                                                  tgttgCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGctct                                                                                                                                                                                     >  2:410140/1‑249 (MQ=255)                                                                   gttgCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTa                                                                                                                                                                                    >  1:417826/1‑249 (MQ=255)                                                                    ttgCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaaa                                                                                                                                                                                  <  2:141488/250‑1 (MQ=255)                                                                          aataaGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaagaa                                                                                                                                                                              <  2:916885/248‑1 (MQ=255)                                                                              aGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaaa                                                                                                                                                                        <  2:107902/250‑1 (MQ=255)                                                                               gAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGTATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATACAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaaa                                                                                                                                                                        >  2:306748/1‑249 (MQ=255)                                                                                 aaaCTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                                                                                                                                       >  2:558010/1‑248 (MQ=255)                                                                                 aaaCTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATaa                                                                                                                                                                     <  1:143304/250‑1 (MQ=255)                                                                                  aaCTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAg                                                                                                                                                                    <  1:454676/250‑1 (MQ=255)                                                                                      gTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAAt                                                                                                                                                                 >  1:607163/1‑249 (MQ=255)                                                                                         tctttcAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                                                                                                                              >  1:759586/1‑249 (MQ=255)                                                                                                 tAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCaa                                                                                                                                                     <  2:594535/250‑1 (MQ=255)                                                                                                  aGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCaaa                                                                                                                                                    <  2:742534/250‑1 (MQ=255)                                                                                                   ggAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCaaa                                                                                                                                                    >  1:54606/1‑249 (MQ=255)                                                                                                       aaTTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACt                                                                                                                                                >  1:141345/1‑249 (MQ=255)                                                                                                           atcatcatcATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACa                                                                                                                                            <  2:27933/249‑1 (MQ=255)                                                                                                                 atcatATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACgg                                                                                                                                     >  2:365105/1‑250 (MQ=255)                                                                                                                  tcatATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGt                                                                                                                                    <  1:503192/250‑1 (MQ=255)                                                                                                                   catATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGt                                                                                                                                    >  1:498050/1‑249 (MQ=255)                                                                                                                     tatTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcat                                                                                                                                 >  1:822458/1‑250 (MQ=255)                                                                                                                         tAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTcatcat                                                                                                                              >  1:918808/1‑249 (MQ=255)                                                                                                                           aCAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCTACGGTCATCatgat                                                                                                                           >  2:270547/1‑250 (MQ=255)                                                                                                                             aaaGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATACCTATACTCAAAAACTAACAGCCACGGTCATCATGAtgt                                                                                                                         >  2:527813/1‑250 (MQ=255)                                                                                                                                gAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGc                                                                                                                      >  2:96650/1‑250 (MQ=255)                                                                                                                                gAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACACAAAAACTAACAGCCACGGTCATCATGATGTGGc                                                                                                                      <  2:380100/250‑1 (MQ=255)                                                                                                                                        cTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATg                                                                                                              <  1:631745/250‑1 (MQ=255)                                                                                                                                          aaTTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGa                                                                                                             >  1:439911/1‑249 (MQ=255)                                                                                                                                            ttGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAAc                                                                                                          >  1:267448/1‑250 (MQ=255)                                                                                                                                              gCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACt                                                                                                         <  2:379600/249‑1 (MQ=255)                                                                                                                                              gCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGAGGCTGTCAATGAAAAta                                                                                                        >  1:230326/1‑250 (MQ=255)                                                                                                                                                 aaaaaTCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATaa                                                                                                     >  2:11328/1‑250 (MQ=255)                                                                                                                                                  aaaaTCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAc                                                                                                    <  1:878634/250‑1 (MQ=255)                                                                                                                                                    aaTCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAccc                                                                                                  <  2:457897/250‑1 (MQ=255)                                                                                                                                                     aTCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCa                                                                                                 <  2:422875/250‑1 (MQ=255)                                                                                                                                                       cGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATAATGATGTGGCTGTAAAGGAAACTATAACCCAgg                                                                                               >  2:350147/1‑250 (MQ=255)                                                                                                                                                         aaGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgcg                                                                                             >  2:167121/1‑250 (MQ=255)                                                                                                                                                               attaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                                                                        >  1:584165/1‑249 (MQ=255)                                                                                                                                                                ttaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTcc                                                                                       <  1:219034/249‑1 (MQ=255)                                                                                                                                                                ttaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTcc                                                                                       <  1:651254/249‑1 (MQ=255)                                                                                                                                                                ttaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCa                                                                                      >  1:144307/1‑250 (MQ=255)                                                                                                                                                                      cccTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacga                                                                                >  2:560546/1‑250 (MQ=255)                                                                                                                                                                       ccTGTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgac                                                                               <  2:362949/250‑1 (MQ=255)                                                                                                                                                                                tCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGt                                                                      >  1:453048/1‑250 (MQ=255)                                                                                                                                                                                      aGTGTCGTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATg                                                                 <  2:868621/249‑1 (MQ=255)                                                                                                                                                                                          tCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCa                                                             <  1:598181/249‑1 (MQ=255)                                                                                                                                                                                              tAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAg                                                        >  1:616559/1‑250 (MQ=255)                                                                                                                                                                                               aGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCGATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGc                                                       >  1:395717/1‑250 (MQ=255)                                                                                                                                                                                                ggAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGcc                                                      <  2:118252/250‑1 (MQ=255)                                                                                                                                                                                                          cTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATc                                            >  1:910377/1‑250 (MQ=255)                                                                                                                                                                                                            ataAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCt                                          >  2:354629/1‑250 (MQ=255)                                                                                                                                                                                                             taAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATcc                                           <  1:331092/248‑1 (MQ=255)                                                                                                                                                                                                                 aTAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaa                                     <  1:605533/250‑1 (MQ=255)                                                                                                                                                                                                                    aGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                  <  2:6093/250‑1 (MQ=255)                                                                                                                                                                                                                    aGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                  >  2:682957/1‑250 (MQ=255)                                                                                                                                                                                                                    aGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                  <  1:188593/250‑1 (MQ=255)                                                                                                                                                                                                                          gtgCTTAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgcctg                             <  2:90576/249‑1 (MQ=255)                                                                                                                                                                                                                              ttAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGATTTCCATAACGACGTCGTCAGTCCATGATAAACCCGCCCCAGCTGATCCTGATACACTGCCTGCCACt                        >  2:873217/1‑250 (MQ=255)                                                                                                                                                                                                                               tAATGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACt                        >  1:443430/1‑249 (MQ=255)                                                                                                                                                                                                                                  tGCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATc                    <  1:176916/250‑1 (MQ=255)                                                                                                                                                                                                                                   gCTTTAAGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATcg                   >  1:578334/1‑250 (MQ=255)                                                                                                                                                                                                                                         aGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa              <  1:410140/249‑1 (MQ=255)                                                                                                                                                                                                                                         aGAAAAATAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa              <  1:752062/249‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCt         >  2:234325/1‑250 (MQ=255)                                                                                                                                                                                                                                             aaaTAGCAATTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCt         <  1:527813/250‑1 (MQ=255)                                                                                                                                                                                                                                                tAGCAAGTCTCCCTGGAATATCGAACTGGTGATGGAAAGATGAATCTGCAAGCTGGCTTTTAACAAGACAGCTCTCAAAGAAGGGAAATACGAATTACAATACTCAAATACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCTAGGCGCGTTCCACATCGACGTCGTTAGTCCACGATCAACCAGACCACGCTGATACCGATAAACCGCCTGCCACCTATCGCGTAAATCCTGag      >  2:369284/1‑249 (MQ=255)                                                                                                                                                                                                                                                    aaTTCTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAg  >  1:319114/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAG  >  NZ_CP009273/1592486‑1592978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑