Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,384,765	A→C	F208C (TTT→TGT)	tldD ←	metalloprotease TldD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,384,765	0	A	C	100.0%	230.4 / NA	68	F208C (TTT→TGT)	tldD	metalloprotease TldD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (46/22);  total (46/22)

CTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑AAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCTTCTCTTCACGGCTCATGCTTTG  >  NZ_CP009273/3384527‑3385009                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGAccgccg                                                                                                                                                                                                                                           <  2:451636/250‑1 (MQ=255)    cccGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGAccgccgccg                                                                                                                                                                                                                                        <  1:630668/250‑1 (MQ=255)     ccGACCGGTCCCCTAAATCCTGAAGGGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACcgccgccgc                                                                                                                                                                                                                                       <  2:856606/250‑1 (MQ=255)      cGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCgccgccgcc                                                                                                                                                                                                                                      <  1:511735/250‑1 (MQ=255)             tCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCa                                                                                                                                                                                                                               <  1:693244/250‑1 (MQ=255)              ccACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCAc                                                                                                                                                                                                                              >  1:570893/1‑250 (MQ=255)               cACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCAcc                                                                                                                                                                                                                             <  1:779927/250‑1 (MQ=255)                    aaTCCTGCAGTTCCACGGCGGTTGAAGTCGACTCCAAGACCGGGA‑CCAACCGCTTCAGTCAACAGCCCGCCCGCCCAACCTGCGCAATGTACTCCGGGCATGGTGCCCGCTGGTGCAGCAGCGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTCCCCATGCATCCGCACGGACTCCCCCGTCGAGATCGGCAAGGAGGAATT‑CATAACCA‑CAACGCCCGCCGCCGCCACTGGCACcgcg                                                                                                                                                                                                                          <  2:923036/248‑1 (MQ=255)                     atccggAAGTGCCACGGCGGTTAAATCCGCCTTCCAG‑CCCTGACCCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCAAAGTCCTACCGGCAGGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACcgcgttc                                                                                                                                                                                                                       <  2:820541/245‑1 (MQ=255)                       aCTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTTCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACcgcggtcgc                                                                                                                                                                                                                     >  1:167954/1‑250 (MQ=255)                       aCTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCCTCATGCAACAGCAAGCCCGGCCAACCTGCGCCCAGTACTACCGGCATGGCGCCCGCCGGGGCAGCAACGGCAGGAAAATTGACC‑AGCGCCATACGCA‑CCGCTTCTTTTGCCCCTGCATCCCCCCCGACCTCCCCCTCGAGAACGGCACGGAAGCATT‑CATAACCA‑CAACGACCGCCCCCCCCACCGGCACcgccctccc                                                                                                                                                                                                                     >  2:324120/1‑244 (MQ=255)                             gTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCCTACGCA‑CTGCTTCTTTTTCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGc                                                                                                                                                                                                               >  1:137852/1‑250 (MQ=255)                                  aCGGCGGTTGAAGTCGCCTGCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtct                                                                                                                                                                                                          <  1:333599/250‑1 (MQ=255)                                      cggTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATcttctt                                                                                                                                                                                                      >  1:229005/1‑250 (MQ=255)                                       ggTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCttcttc                                                                                                                                                                                                     >  2:785901/1‑250 (MQ=255)                                       ggTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCttcttc                                                                                                                                                                                                     >  2:3877/1‑250 (MQ=255)                                        gTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCttcttc                                                                                                                                                                                                     >  2:894804/1‑249 (MQ=255)                                          tGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCTCCGTCGAGATCGGCAAGGACGCATT‑CATAACCA‑CAACGACCGCCGCCGCCCCTGCCACCGCGATCGCGTTTGCCATCTTCTTcga                                                                                                                                                                                                   >  2:742088/1‑249 (MQ=255)                                            aaGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCACCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                                                                                                                                 <  1:928893/249‑1 (MQ=255)                                             aGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCCCCGCGTTCGCGTTTGCCATCTTCTTcgac                                                                                                                                                                                                  >  2:466539/1‑247 (MQ=255)                                               tCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATCCGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGTTCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGaa                                                                                                                                                                                             <  1:333881/250‑1 (MQ=255)                                                 gCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCAACCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCT‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                                                                                                                            <  1:315223/249‑1 (MQ=255)                                                     tCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCa                                                                                                                                                                                       >  2:889610/1‑250 (MQ=255)                                                           ccGGGA‑CCCACCGCTGCATGCAACAGCGCGCGCGGCCAACCTGCGCCAAGTACTACCGGCAGGGTCCCCGCGGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGACATGCGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGCCGAGATCGGCAAGGAAAAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGaaa                                                                                                                                                                                 <  2:104316/250‑1 (MQ=255)                                                                        gCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCCCGGAAAGACGCACCAGcg                                                                                                                                                                     >  2:862813/1‑250 (MQ=255)                                                                            cATGCAACAGCCCGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                                                                                                                   <  1:617270/248‑1 (MQ=255)                                                                            cATGCAACAGCACGCACGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATATTCTTAGACGAGAACGCTCACGGAAAGACTCACCAGcggacg                                                                                                                                                                 >  2:732607/1‑250 (MQ=255)                                                                              tGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacgga                                                                                                                                                               >  2:894700/1‑250 (MQ=255)                                                                                      cACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgcc                                                                                                                                                       >  1:477109/1‑250 (MQ=255)                                                                                       acccccGCCCAACTGTCGCCAAGTACACCCGGAATGTTGCCCGCTGG‑ACAACCACGGCAAAAAAAATGGCCAACCGCCAACCCCA‑CTGCTTCTTTTCCCCATGCCCCCCCACGCCCTCCTCCGCAGAGATGGGAAAGAAAAAATT‑CAAAACCC‑CAACGCCCCCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgccg                                                                                                                                                      <  2:481709/247‑1 (MQ=255)                                                                                         gCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCcgccgc                                                                                                                                                     >  1:723147/1‑249 (MQ=255)                                                                                         gCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCcgccgc                                                                                                                                                     >  2:817877/1‑249 (MQ=255)                                                                                             ggCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCg                                                                                                                                                >  2:128918/1‑250 (MQ=255)                                                                                                   ccTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGt                                                                                                                                          >  1:432596/1‑250 (MQ=255)                                                                                                             gTACTACCGGCAGGGCGCCGGGTGGAGCGGCAGCGAAAAAAAGATGGCCC‑AGC‑CCCTGCCCACCTTCTTCTTTTCCCCAGCCCCCCGCACGGCCTCCGCCGCCGAGA‑CGGCAAGGAAAAATTCCAAACCCA‑CAACGCCCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGc                                                                                                                                <  2:802043/250‑1 (MQ=255)                                                                                                               actacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCaa                                                                                                                              >  1:46565/1‑250 (MQ=255)                                                                                                                      gCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGCCGGTGGCCGCCACCaaaa                                                                                                                        >  2:581018/1‑249 (MQ=255)                                                                                                                        aTGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatt                                                                                                                      >  1:924392/1‑249 (MQ=255)                                                                                                                         gggcgCCCGCGGGTGCAAAACCGGAAAAAAGATGCACA‑AGCACCAGCCCCC‑CGTCTCCTTGTCCCCACGCACCCGCAGGGACTCCGCCGCGGAACTCGG‑AAAGAAAAAAT‑CAAAACCACCACCGCCCCCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCCCCAGCGAACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                                                                                                                    <  2:216897/246‑1 (MQ=255)                                                                                                                               ccGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCa                                                                                                                >  1:797969/1‑248 (MQ=255)                                                                                                                                cGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATtcattcatc                                                                                                              >  2:926014/1‑248 (MQ=255)                                                                                                                                cGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCa                                                                                                                >  1:271187/1‑247 (MQ=255)                                                                                                                                     gTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGacac                                                                                                         >  1:586386/1‑249 (MQ=255)                                                                                                                                        cagcaACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAACTTACTTCATAGACACCACt                                                                                                     >  2:115030/1‑250 (MQ=255)                                                                                                                                        cagcaACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACt                                                                                                     >  1:384277/1‑250 (MQ=255)                                                                                                                                        caccaccGGCAGAAAGATTCACC‑CGCGCCATCCGCA‑CTCCTTTTTTCCCCCATGCCCCCGCAGGCACCCGCCCGCCGGGGCCGGCAAGGAAGAATA‑CAAACCCA‑CCACCCCCCCCGCCCCCACTGGCCCCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACt                                                                                                     <  2:907963/244‑1 (MQ=255)                                                                                                                                             aCGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTCCTCATCCATACGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATACACACCACTGCGGc                                                                                                >  2:91667/1‑250 (MQ=255)                                                                                                                                                 cAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTgg                                                                                             >  1:606404/1‑249 (MQ=255)                                                                                                                                                      aGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCa                                                                                       >  2:235917/1‑250 (MQ=255)                                                                                                                                                      aGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGATGGCAGTCa                                                                                       >  1:225275/1‑250 (MQ=255)                                                                                                                                                             cc‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTCATTCATAGACACCACTGACGCTGGCAGTCACTTCCTg                                                                                >  2:785828/1‑250 (MQ=255)                                                                                                                                                                           cA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTcc                                                                   >  1:918900/1‑250 (MQ=255)                                                                                                                                                                              cTGCTTCTTTCCCCCATGCACCCGCACGGACTGCCCCGTCGAGATCGGCAAGAAAAAATT‑CAAACCCA‑CAACGCCCCCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGc                                                                 <  1:214443/250‑1 (MQ=255)                                                                                                                                                                                  ttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAACATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTcg                                                             >  2:96365/1‑250 (MQ=255)                                                                                                                                                                                     ttttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCgg                                                          >  1:62311/1‑250 (MQ=255)                                                                                                                                                                                                 aTCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATc                                               >  1:56918/1‑249 (MQ=255)                                                                                                                                                                                                 aTCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATc                                               >  1:280973/1‑249 (MQ=255)                                                                                                                                                                                                     gCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATcgacgc                                          >  1:62021/1‑250 (MQ=255)                                                                                                                                                                                                       aCGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATcgacgcga                                        >  2:302717/1‑250 (MQ=255)                                                                                                                                                                                                              tcccggtggagaTCGGCAAGAAAAATTC‑CAAACCCC‑CACCGCCCCCCCCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAg                                   <  1:47330/240‑1 (MQ=255)                                                                                                                                                                                                                cccGTCGATGTCGGAAAGGAAGAATT‑CATACCCA‑CACCCCCCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGata                               <  1:894804/249‑1 (MQ=255)                                                                                                                                                                                                                  cGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATc                             >  1:54563/1‑250 (MQ=255)                                                                                                                                                                                                                    tCGCGATCGGCAAGGAAGAATT‑CATCACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCAATCTTCTTCGACGAGACCGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCCATCAAAATTAATTCATAGACAACACTGTGGCTGGCAGGCACTTCCTGTACGCGCTTGTCCGCTTCTCGGGCAACCTTATCGACGCGACGCAGGATATCCa                           >  2:298834/1‑250 (MQ=255)                                                                                                                                                                                                                      gagaTCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGc                         >  1:100398/1‑250 (MQ=255)                                                                                                                                                                                                                       agaTCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCt                        >  1:218704/1‑250 (MQ=255)                                                                                                                                                                                                                        gaTCGGCAAGGAAGAATT‑CATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCtt                       >  1:436233/1‑250 (MQ=255)                                                                                                                                                                                                                                           cATAACCA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCTTCTCTTCACGGCTCATGc      <  1:427084/249‑1 (MQ=255)                                                                                                                                                                                                                                                ccA‑CAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTATCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCTTCTCTTCACGGCTCATGCTTTg  >  2:622565/1‑248 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGA‑CCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACC‑AGCGCCATACGCA‑CTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATT‑CATAACCA‑AAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGCAACCTTATCGACGCGACGCAGGATATCCAGCTTCTCTTCACGGCTCATGCTTTG  >  NZ_CP009273/3384527‑3385009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑