| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,621,979 | 0 | T | A | 33.9% | 97.8 / 19.4 | 62 | T107S (ACT→TCT) | pdeF | cyclic‑guanylate‑specific phosphodiesterase PdeF |
| Reads supporting (aligned to +/- strand): ref base T (30/11); new base A (0/21); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.78e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCGAATGATGAAGTAGCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAATGTGGGCGAAAATGGGCAAGATT > NZ_CP009273/2621733‑2622226 | cccGAATGATGAAGTAGCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTaa < 1:487514/249‑1 (MQ=255) aaTGATGAAGTAGCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGAcc < 2:726295/250‑1 (MQ=255) aGTAGCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAAcc > 2:153574/1‑250 (MQ=255) gCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGccc < 2:488357/250‑1 (MQ=255) gCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGcc < 1:387457/249‑1 (MQ=255) acaGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGcccc > 2:680288/1‑249 (MQ=255) caGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCa > 2:219574/1‑249 (MQ=255) gATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCACCAACAGAGaatgat > 2:555211/1‑249 (MQ=255) aTTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGaatgataatgat > 2:622949/1‑249 (MQ=255) ttACCCACCAGTAAGGCCTGATAATTAATTAAAGTCCCGAGGTAAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAAGGCAGAAAACTGAAAAAGTATCAGAAACAGCGTT‑GCAGAAAACACAA‑T‑CTGCCAGAATATACGCTGGAAAATTAAACGGGTACCACCATGTG‑AACC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAAtgataatgataa < 1:134232/250‑1 (MQ=255) cccACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGataata > 2:739285/1‑249 (MQ=255) tAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc > 1:693143/1‑250 (MQ=255) aGGCATGATAATTAATTAAGGCCCGGAATAAAAAAGCCATGACACCAACCAGATGTTCTCTGCCCGCCAGTAACCCAACAAAGGAAGCAAAAGGAAAAAGAATCAGAAACTGCGTA‑GAAGGAAACAAAA‑T‑CTACAGGAATTTACGCGGGGAAATAAAACGGAAACCGCCATATA‑AAAC‑GTTGTTACGCCGGGGATTAAAACCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGt < 2:518583/250‑1 (MQ=255) ctaaTAATTGATTAAAGCCCAGAGGTTAAAAGGCATGACACCGCCCAGATTTTCTCTGCTCGCAAGTAATCCTAAAAATGCAGAAAACTGAAAAAGTATCAGAACCAGCGTT‑GCAGAAAACACAA‑T‑CTGCAAAAATATACGCTGGGAAATTAAACGGGTACCACCATGTG‑AAAC‑GTTGTTCCGCGGGGGAGAAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAAt < 1:137477/247‑1 (MQ=255) aTAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa > 1:394163/1‑249 (MQ=255) aTAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa > 1:540105/1‑249 (MQ=255) tAATTGATTAAAGTCCCGAGGATAAAAGGCATGACACCGACCAGATTTTCTCTACTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACTC‑TGTAACCGACCCAATGGAGAATGGTCATGATAGTAAACTTAGCCGTAACTGa > 2:192385/1‑248 (MQ=255) tttGATAAAATCCACGAGTTAAAAGGGCATGACACCGACAAGTGTTCCTCTGCACGCAAGTAATCCAAAAAAGGAAAAAAATGGAAAAAGAAACAAAAAAAGCGTT‑GCAGGAAAAAAAA‑T‑CGGCAAAAAAAAACGCGGGAAAAAAAAACGGGTATCCCCATGTA‑AAAC‑GTTTTTACGCGGGGGAGAAAAGCCCT‑GGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGc < 2:630941/249‑1 (MQ=255) ttGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCg > 2:170668/1‑250 (MQ=255) aTTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACCGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGttt > 2:336759/1‑250 (MQ=255) actccgtagttaaaaaGGCAGCACACCGACAAGATTTTCTCGGTCCGCCAGAAATGCTACAAATGAAGAAAACTGGAAAAGTATCAGAAACATGGTG‑GCAGGAAACACAA‑T‑CGGAAATAAAATGCGCTGGGAAATACAACGCTTACCCCCAGGTA‑AAAC‑TTTTTAACGCGGGGGATAAAAGCCTT‑ATTATCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaa < 2:710479/238‑1 (MQ=255) aaaaGGCATGACCCCGACCAGATTTTCTCTGCCCCCCAGTAATCCTAAAAAGGAAGCAAACTGAAAAAGTATCAAAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATCCGCTGGAAAATAAAACGGGTATCCCCATGTG‑AAAC‑GTTGTTCCGCCGGGGAGAAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctct < 1:112534/250‑1 (MQ=255) aaaaGACATGACCCCCACCATATTTCCTTCGCTCCCAAGTAATCCTAAAAAGCAAGAAAACT‑GAAAAATATAAAAAAACGGGTTGGCAGAAAACACAA‑T‑CTCCAAGAATATACGGTGGGAAATAACCCGGTTATCCCCATTTA‑AAAT‑TTTTTTCCGCGGGGAAAAAAAACCCC‑TATAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctct < 2:608972/250‑1 (MQ=255) aGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCa > 2:334396/1‑250 (MQ=255) aGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCa > 2:228033/1‑250 (MQ=255) aGGCATAACCCCGACCATATTTTCTCGGCTCGCCAGTAATCCTAAAAATGAAGAAACCGGGAAAAGTATCAAAAACAGCTTT‑GCAGAAAACAAAA‑T‑CTGCCAGAATATACGCGGGGAAATAAAACGGGTATCCCCATGTA‑AAAC‑GTTGTTACGCCGGGGAGAAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCa < 1:114625/250‑1 (MQ=255) acacCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc > 1:403867/1‑250 (MQ=255) cacCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCg > 2:586893/1‑250 (MQ=255) gACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGAc > 2:646676/1‑249 (MQ=255) aCCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACa > 2:89943/1‑249 (MQ=255) tccttGCTCGACAGAAATCCAACAAACAAAACAAACTGAAAAATTATCAGAAACAGCGTT‑GCAGAAAACACAA‑T‑CTGCCAGAATATACGGGGGAAAATAAAACGGGTATCCCCATGTA‑AAAC‑TTTGTTACGCCGGGGAGAAAACACCC‑TGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt < 1:105755/245‑1 (MQ=255) ggCTCGCCAGTAACCCAAAAAATGAAGAAAACGGAAAAAGTATCAGAACCAGCGTT‑GCA‑GAAAAACAATT‑CGGCAAAAATATACGCTGGAAAATAAAACGGGTATCACAATGTA‑AAAC‑GTTGTTCCGCCGGGGAGAAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTaa < 1:604106/246‑1 (MQ=255) aTGCAGAAAACTAAAAAAGTAACAGAAACAGCTTG‑GCAGAAAACACAA‑T‑CTGCGAGAATATACGGTGGAAAATAAAACGGGTACCACCAGGTA‑AAAT‑TTTGTTCCGCGGGGGAGAAAAGCCCC‑TGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCa < 2:624742/250‑1 (MQ=255) aacggaaaaaGTATAAGAAACAGCGTT‑GCAGAAAACACAA‑C‑CTGCAAGAATATACGCGGGAAAATAAACCGGTTCCCCCCATGTG‑AAAT‑TTTGTTACGCGGGGGAGAAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaa < 2:307295/244‑1 (MQ=255) cgtgaaatagaacAGAAACAACGTT‑AGAAGGAACCCAA‑TCCGGCAAGAATATACG‑TGGGAAAATAAACGGGGTACCTCATGGT‑AAAA‑CTTGTTTCCGCGGGAAATAAAGCCCCTTGAATCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa < 2:157001/238‑1 (MQ=255) aaCAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGatca > 1:624046/1‑250 (MQ=255) gCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAg > 2:618912/1‑250 (MQ=255) gTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTg > 2:345705/1‑250 (MQ=255) aaaCACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAgg > 2:473937/1‑249 (MQ=255) cacaA‑T‑CTGCCAGAATATCCGCTGGGAAATTAAACGGGTATCACCATGTA‑AAAC‑TTTGTTACGCGGGGGAGAAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGa < 1:205897/250‑1 (MQ=255) acaA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGa > 2:174193/1‑249 (MQ=255) caA‑G‑CTGCCAAAATAACCGAGGGAAAATAAAACGGGTACCACCAGGTG‑AAAC‑GTTTTTACGCCGGGGAGAAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATa < 2:117855/250‑1 (MQ=255) gAATATACGGAGGAAAATAAAGCGGGTACCCCCATGGA‑AAAC‑GTTGTTACGCGGGGAAGAAAAGCCCC‑TTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGaaaa < 1:406151/250‑1 (MQ=255) gAATATACGCGGGAAAATTAACCGGGTACCACCATGTG‑AAAC‑TTTGTTCCGCCGGGGAGAAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGaaaa < 1:716938/250‑1 (MQ=255) aCGCTGGAAAATTAAACGGTTATCCCCATGAA‑AAAC‑TTTGTTACGCGGGGGAGAAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCa < 1:85758/250‑1 (MQ=255) gCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCAcc > 1:285891/1‑250 (MQ=255) tGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCg > 1:683333/1‑249 (MQ=255) gggAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCgg > 1:154942/1‑249 (MQ=255) ggAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCgg > 2:89033/1‑248 (MQ=255) ggAAATAAAACGGGAATCCCCA‑GTGAAAAT‑GTTGTTACGCCGGGAATAAAAGACCC‑GGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGgaa < 1:197910/250‑1 (MQ=255) aaaaTAAACCGGGTATCCCCAGGTG‑AAAC‑GTTGTTACGCCGGGGAAAAAAACCCC‑TTAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGgaag < 2:671945/249‑1 (MQ=255) aaaTTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGgaag > 2:530262/1‑249 (MQ=255) aaaTAAAAGGGTTACCACCA‑GGG‑AAACGGTGGTGACGCGGGGGAGAAAAGACCT‑GGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGgaaga < 1:229179/250‑1 (MQ=255) aaagcGGGTAACCCCATGAG‑AAAC‑GTTGTTACGCGGGGAAGTAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAata < 2:464881/246‑1 (MQ=255) aaaCGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAatat > 2:622324/1‑250 (MQ=255) aCGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATgc < 1:345705/250‑1 (MQ=255) gggTACCCCCATGAG‑AAAC‑GTTGTTACGCCGGGGAATAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATgcgc < 1:438871/250‑1 (MQ=255) ggTATCACCATGGA‑AAAC‑GTTGTAACGCGGGGGAGAAAAGACCC‑TGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGcgcg < 2:615642/250‑1 (MQ=255) ccATGAG‑AAAG‑TTTTTTACGCGGGGGAAAAAAATCCC‑CTAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTAt < 2:342976/250‑1 (MQ=255) aaaC‑GTTGTTACGCCGGGGAGTAAAGCCCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAAtgt < 1:176567/249‑1 (MQ=255) tttgttACGCCGGGGATAAAAGCCCC‑TGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAATGTGGGCg < 2:698680/249‑1 (MQ=255) gTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAATGTGGGCGAAAATGGGCAAGAtt > 1:738066/1‑249 (MQ=255) | CCCGAATGATGAAGTAGCACAGCGGGACACCGATCAGATTACCCACCAGTAAGGCCTGATAATTGATTAAAGTCCCGAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTT‑GCAGGAAACACAA‑T‑CTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTG‑AAAC‑GTTGTTACGCCGGGGAGTAAAGACCC‑TGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAACATCATCATGCTGATCATCAGTGCCAGAGGCAGATAAAAGAGAAAAACCTCACCGGAAGAAATATGCGCGAAAGTATTAATGTGGGCGAAAATGGGCAAGATT > NZ_CP009273/2621733‑2622226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |