Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,245,9970GC44.3% 89.8 / 29.7 61P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
Reads supporting (aligned to +/- strand):  ref base G (10/24);  new base C (27/0);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 2.98e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCT  >  NZ_CP009273/4245749‑4246242
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
gATCCTGGCGAGCAGGCAACCGTGGGCCAACGGCAGCGAGCCGTGGAGGGGCAAAGGGCCTCCGCGCCCCGCGCGCCAGTTTCTGAGGGATAGTTTAATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCgg                                                                                                                                                                                                                                                         <  2:456778/250‑1 (MQ=255)
 aTCCGGCCGAGCAGGCAAACGGGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCTTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgc                                                                                                                                                                                                                                                        <  2:745309/250‑1 (MQ=255)
   ccTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcgc                                                                                                                                                                                                                                                      <  2:205971/250‑1 (MQ=255)
         gAGCAGGCAAACGTGGGCCTACGGCAGCCATACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGAcc                                                                                                                                                                                                                                                <  1:258101/250‑1 (MQ=255)
                cAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCAAACAt                                                                                                                                                                                                                                          >  1:312249/1‑249 (MQ=255)
                 aaaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCCAACATCa                                                                                                                                                                                                                                        >  1:182150/1‑250 (MQ=255)
                     gTGGGCCTACGGCAGCCAGTCGTTGAGGGGAAAAGTGCATGCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTAGCCGTGCCGTTTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGa                                                                                                                                                                                                                                    <  2:535094/250‑1 (MQ=255)
                        ggCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCTCCTTTTTTTCCCCCCCGCGCGCCGCCACACACCACCCaca‑c                                                                                                                                                                                                                                >  2:302586/1‑250 (MQ=255)
                                       gACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTTCACGCCCCCGCGCGCGACCCCAAATCACCCTCC‑CTGGCACCATCTGcac                                                                                                                                                                                                                 >  1:209532/1‑250 (MQ=255)
                                        aCGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGcaca                                                                                                                                                                                                                <  1:86662/250‑1 (MQ=255)
                                           ttGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATcc                                                                                                                                                                                                             <  1:300745/250‑1 (MQ=255)
                                            tGACGGGCAAGGTGCCTCCGCGCCACGCGCGCCGGTTTCTGAGCGATAGTTTTACCCGTCCCGTTTCCATCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCa                                                                                                                                                                                                            <  2:521444/250‑1 (MQ=255)
                                                       gTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCGCCA‑CCGGCACCCTCTGCACATCCAGATTTggggtt                                                                                                                                                                                                 >  1:509279/1‑247 (MQ=255)
                                                         gCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGGCACTTCGCCTTTTTTTCGCCCCCGCGCGGGGACCAACAACCCCGCCA‑CCAGCACACTCTGCACACCCCGGATTGGGGTggt                                                                                                                                                                                               >  2:301314/1‑248 (MQ=255)
                                                                 gcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCa                                                                                                                                                                                       <  1:631252/250‑1 (MQ=255)
                                                                  ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCaa                                                                                                                                                                                      <  1:105562/250‑1 (MQ=255)
                                                                  ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTTACCCCCCCGCGCGCGGCCAAACAACACCCACA‑CCCGCCCCCTCTGCCCACCCACAATTTGGTTTCTACGGTGCa                                                                                                                                                                                       >  2:734977/1‑249 (MQ=255)
                                                                   cACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTTCCCTTTTTTACCCCCCCGCGCGCGGCCCAACATCACCCCCA‑CTGGCACCCTCCGCACAACCACATTTTGGGTGTTAAGGGGCcaa                                                                                                                                                                                     >  1:659108/1‑250 (MQ=255)
                                                                   cACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTTTACGCCCCCGCGCGCGGCCAAACATCACCGACA‑CCGGCACCATCTCCCCCTCCCCATTTGGGGTGCTACgggggac                                                                                                                                                                                      >  2:465997/1‑244 (MQ=255)
                                                                           ccAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTCCTTTGCCTTTTTTACGCCCCCGCGCGCGGCCCAAAATCACCCACA‑CTGGCACCCTCTTCACATCCAGATTTGGGTTGCTACGGTGCAAATCgaga                                                                                                                                                                               >  1:330533/1‑248 (MQ=255)
                                                                                ttCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCCTCTCCTTTTTCTACCCCCCGCGCGCGGCCCCAAATCACCGCCA‑CTCGCCCCCTCTGCACATCCCGATTTGGGTTTCTACTGTGCAAATCGCGATTGTAGt                                                                                                                                                                        >  2:688797/1‑250 (MQ=255)
                                                                                ttCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCg                                                                                                                                                                         <  1:745045/249‑1 (MQ=255)
                                                                                 tCTGAGCGATAGTTTTACCCGTGCCGGGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGt                                                                                                                                                                        <  1:495518/249‑1 (MQ=255)
                                                                                    gAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGGTACTTCTCCTTTTTTCTGCCCCCGCGGCCGGCCCAACAACACCCCCA‑CCCGCACCACCTGCCCCACCCGATATTGGGTGCTACGGTGCCCACCGGGATAGGCGTgtg                                                                                                                                                                     >  2:987/1‑247 (MQ=255)
                                                                                     aGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGa                                                                                                                                                                     <  1:623587/248‑1 (MQ=255)
                                                                                     aGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGa                                                                                                                                                                     <  1:185185/248‑1 (MQ=255)
                                                                                           aGTTTTATCCGTGCCGTGTTCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa                                                                                                                                                             <  1:743851/250‑1 (MQ=255)
                                                                                             ttttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAAAATAACCGACA‑CCGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGGACAGCTTaa                                                                                                                                                            >  1:217338/1‑249 (MQ=255)
                                                                                                  tCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCAACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCCGCCCCCGACCCAACAACACCCACA‑CCGGCACCCACCGCCCAACCAACATTTGGTGGTTACCGTGCACAACAAGATAATCGTGGAACAACTTACTAgcac                                                                                                                                                      >  2:435871/1‑247 (MQ=255)
                                                                                                         cgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                               <  1:433946/250‑1 (MQ=255)
                                                                                                               cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTCCGCCCCCGCGCGCGACCAAACAACACCCAAC‑CTGGCCCCATCCTCACACCCACCTTTGGGTTGCTTCGCTGCAAATCGAGCTAGTCGTGGAACAGCCTTATAAACTCTTCTTTTGGCgt                                                                                                                                         >  2:416443/1‑250 (MQ=255)
                                                                                                                aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGGTACCTCCCCCTTTTTTCGCCCCCGCGCCCCCCCCAACACCACCCCCC‑CTCGCCCCACCTGCACACCCCCATTTGGGTTGGTTCCGGGCAAAAAGAGATAGTCGTGGGAACGCTTTATATAACCTTTTTTCTGCgcg                                                                                                                                        >  2:698593/1‑248 (MQ=255)
                                                                                                                 tCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtgt                                                                                                                                       <  1:627830/250‑1 (MQ=255)
                                                                                                                   cGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACCCCCCGGCGCGCGACCACACATCACCGACA‑CCGGCACCACCCGCACATCCCGATTTTGGTTGCTACGGTGCAAAACGAGATAGTCGTGGGACAGCCTAATAGACTCTTCTTTCTGCGTGTa                                                                                                                                      >  2:192926/1‑249 (MQ=255)
                                                                                                                     ccATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTCCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATa                                                                                                                                   <  1:460601/250‑1 (MQ=255)
                                                                                                                          cgccgcAGCGAAGCTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGt                                                                                                                                <  2:82847/245‑1 (MQ=255)
                                                                                                                                              cGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCCCCCCCCCGCGCGCGACCAAAAAACACCCGCA‑CTGGCACCCTCTCCACAACCACATTTTGGGTGGTACGGGGCAACTCGAGATAACGTTGGGACAGATTTATAGACTCTTCTTTTTGCGCGTCATTGGTGAACAAACGC‑GGCCCCCCCtg                                                                                                          >  2:87876/1‑249 (MQ=255)
                                                                                                                                                      gCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGGAATAGGGGAAAAAACGC‑GGGCCGCCGCGAATG‑Aaca                                                                                                 >  1:416916/1‑249 (MQ=255)
                                                                                                                                                       cacaAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTCCCCTTTTTTCCACCCCCGCGCGCGCCCCAAAAACACCGGCC‑AAGGGACCATCTGGTCACACAGATTTGGGGTGCTATGGTGCAAAACGCGAATGGTGGGGTAAAACTTAATAGACACTACTTTCTGCGGGTAATAAGGGATAACACAC‑GGCCCGCCCTGGATT‑cacaca                                                                                               >  2:537652/1‑250 (MQ=255)
                                                                                                                                                                 ccgcgCCCGACCCACAGTACAGCAAAAATTTTCTGTCCGCCGTAAAGCATCCGCAGCGGCGGTTTCCCTTCGCTTTTTCCCCGCCCCGGCGCGCGACAAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCgg                                                                                       <  1:339527/247‑1 (MQ=255)
                                                                                                                                                                             cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCGACA‑CTGGCCCCATCTGCACATCCCGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCCGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGGGCCGt                                                                         >  1:172563/1‑250 (MQ=255)
                                                                                                                                                                             cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTTCACGCCCCCGCGCGCGACCAAACAACACCGACA‑ACGGCACCAACTGGACATCCCGATTTGGGGTGGTACGGGGGAAAACGGGAAAGAGGTGGAAAAGCTTAAAAAAATCTTCTTTCGGCGGGTGAAAGGGGGAAAAAAGC‑GGCCCGCCCGGAAAG‑AACACAAAGCGCGGGAGCGGCGTGTCgg                                                                         >  2:679605/1‑249 (MQ=255)
                                                                                                                                                                               caGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTTGCCTTTTTTTCCCCCCCGCGCGGGAACAAACATCAACCACA‑CTGGCACCATCTGCCAATCCAGATTTGGGGTGCTACGGGGCAAATCGAGATAGTCGTGGAAAAGCTTAATAGACTTTTCTTTCGGCGTGTAATAGGGGAAAACACGC‑GGGCCCCCGTGAATGCAACA‑ATAGGGCGGGCGCAGCGGGCCGGCg                                                                       >  2:513749/1‑250 (MQ=255)
                                                                                                                                                                                    cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCTTTTTCACCCCCCCGCGCGCGACCCAACAACACCCACA‑CTGGCACCATCTTCACATCCCAATTTTGGTTGGTACCGTGCAAATCGAGATAGTCGTGGGACAGCCTAAAAGACTCTTCTTTCTGCGGGGAATAGGTGAACACACAC‑CGCCCCCCGTGAATG‑AACACACAACGCTGCAGGAACGTGCCCGCGCTTTc                                                                  >  2:565320/1‑250 (MQ=255)
                                                                                                                                                                                    cAGCAAAAAATTTCTGTACGCCTTTAAGCATACGCAGCGGCGGGTTCACTCCCCTTTTTCCACCCCCCGGCGCGCGACAAAACACCCCCGACA‑CGGGCACCATCTGCCCATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTc                                                                  <  1:648490/250‑1 (MQ=255)
                                                                                                                                                                                     aGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACATACCCTTTTTCACGCCCCCGCGCGCGAACCAACAACCACCACA‑CCCGACCCATCCTCCCATCCACATTTTGGTTGGTACGGGGCAAATCGAAATAATTGTGGGACAACTTAAAAGAACATTCTTTTCGCCGGTAAAAAGTGAAAACACACAGCCCCCCCGGGATTG‑AACACA‑CGCGCAGGAGGAGGGGTACGTCGATTTcc                                                                 >  2:384775/1‑250 (MQ=255)
                                                                                                                                                                                          aaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCCACA‑CTGGCACCATCTGCACATCCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGGGAACACACGC‑GGCCCGCCGTGAATG‑AAAACATAGCGCGGCAGTAGCGTGCCGTCGCTTTTCCGCgg                                                            >  1:544100/1‑250 (MQ=255)
                                                                                                                                                                                            aaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTTTCCCCCCCGCGCGCGACCCAACATCACCCACACCTGGC‑CCATCCTCCCCTCCAGATTTTGGTTGCTCCCGGGCAAAACGAGGTAGTCGTGGAACAGCCTAATAGACTCTTTTTTCGGCGTGTAATAAGGGAACACACGC‑CGCCCCCCGTGGATT‑AACACCAAACGCCGCCGTAGGGGGCCCGCGGTTTCCACCGGct                                                          >  2:548498/1‑250 (MQ=255)
                                                                                                                                                                                              tttCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCCAACATCACCGACA‑CCGGCACCATCTGCACCTCCAGAATTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGGAAAGCCTAATAGACTCCTCTTTTGGCGTGTTATAGGGGAAAACACGC‑GGCCCCCCGTGAATG‑AAAACCTAGCGCGGCACTAGCGTGCCGCCGGTTTCCCGCGGcgc                                                         >  2:633715/1‑247 (MQ=255)
                                                                                                                                                                                                 cTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGGCCAAACATAAACGACA‑CTGGCAACCTCCGCACAACCAGATTTTGGTTGCTACGGTGCAAAACGAGATAATCGTGGGACAGCCTAATAAAATCTTCTTTCGGCGGGTGATAGGGGAAAACACGC‑CGCCCCCCGGGAAAG‑AAACAATAAGGCGGCGGGCAAGTGCCCGCGACTTCCAGCAGctctc                                                       >  2:667582/1‑247 (MQ=255)
                                                                                                                                                                                                       gCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCCAACATCACCCACA‑CCCGCCCCCTCTGCACATCCAGATTTGGGTTGCTACGGGGCCAAACGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAAAACACCC‑GGCCCGCCGTGAATG‑AACACCTAGCGCGGCAGTAGCGGGCCGGCGCTTTCCCGCGGCCCTAACagggcc                                               >  2:731711/1‑247 (MQ=255)
                                                                                                                                                                                                                             gggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACt                         >  1:704385/1‑250 (MQ=255)
                                                                                                                                                                                                                              gtttcccttcccCTTTTGCCCGCCCCGGCGCGCGACAAACCACCACCGACA‑CTGGCACCATCTGCACATCCAGATTGGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTg                        <  2:14475/248‑1 (MQ=255)
                                                                                                                                                                                                                                  cACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcg                    >  2:527364/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aCTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc                   >  2:673185/1‑250 (MQ=255)
                                                                                                                                                                                                                                       ccccTTTTCCCCCCCCCGGCCCCCGCCAAACCACCCCCGACA‑CTGGCCCCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTTGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCa                <  1:273919/247‑1 (MQ=255)
                                                                                                                                                                                                                                        tccTTTTTCCCCCCCCGGCGCGCGCCCAAACATCACCGACA‑CTGGCACCATCTGCACATCCATATTTGGTTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGGGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCa                <  1:147525/247‑1 (MQ=255)
                                                                                                                                                                                                                                        gCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc              >  2:348884/1‑250 (MQ=255)
                                                                                                                                                                                                                                           tttttCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGt            >  2:291372/1‑249 (MQ=255)
                                                                                                                                                                                                                                             tttCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATGGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAg         >  1:259907/1‑250 (MQ=255)
                                                                                                                                                                                                                                               tCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGc        >  1:702774/1‑249 (MQ=255)
                                                                                                                                                                                                                                                    ccccGGCGCGCGACAAAACATCCCCGACA‑CGGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACACCCGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCt  <  1:578094/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
GATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACA‑CTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGC‑GGCCCGCCGTGAATG‑AACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCT  >  NZ_CP009273/4245749‑4246242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑