Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273328,7110AG34.4% 111.2 / 17.3 64G210G (GGA→GGG) pdeLDNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
Reads supporting (aligned to +/- strand):  ref base A (5/37);  new base G (22/0);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 1.00e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGAGT  >  NZ_CP009273/328464‑328960
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
cGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGAtt                                                                                                                                                                                                                                                           <  1:595314/250‑1 (MQ=255)
   ttttCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGGTTTg                                                                                                                                                                                                                                                         >  1:536765/1‑249 (MQ=255)
       cTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGGGGGGTTTGgaaa                                                                                                                                                                                                                                                     >  1:625794/1‑249 (MQ=255)
          cgcgcgtagtGGCGTACTGACGGGCTGGGAGGTGCTTGTCCGCTGGAAACATCCACAAACGGTAATTATCCCACCGGATCAGTTTATTCCTCTGGCAGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGAGGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATAAACGTCTCGGCGGGTTGTTGTTTGGCAGCGGGATGTGaaaaa                                                                                                                                                                                                                                                    <  2:497897/238‑1 (MQ=255)
          cgcgcAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAaga                                                                                                                                                                                                                                                  <  2:381843/249‑1 (MQ=255)
           gcgcAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCCGGGGGGGTTGtaaaaaa                                                                                                                                                                                                                                                  >  1:161423/1‑246 (MQ=255)
                     gCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCt                                                                                                                                                                                                                                     <  2:416553/250‑1 (MQ=255)
                      cGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTg                                                                                                                                                                                                                                    <  2:26398/250‑1 (MQ=255)
                        tACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGAttt                                                                                                                                                                                                                                                          <  1:103485/227‑1 (MQ=255)
                             aCGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTtaat                                                                                                                                                                                                                              <  1:257951/249‑1 (MQ=255)
                              cGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTtaataa                                                                                                                                                                                                                            <  2:651784/250‑1 (MQ=255)
                                   tgtgAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGATTTGTAAAAGAGTG‑TCCTAAACTGGTTTATAAAtttg                                                                                                                                                                                                                       >  1:428977/1‑248 (MQ=255)
                                     tgAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAgg                                                                                                                                                                                                                      <  2:119016/249‑1 (MQ=255)
                                            cTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGAt                                                                                                                                                                                                               <  1:746941/249‑1 (MQ=255)
                                                tCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCCGCGGGGTTTGAAAAAGAGGG‑TCTTAACCTGGTTTATAAATTAGGTAACGATaaaa                                                                                                                                                                                                           >  1:664091/1‑249 (MQ=255)
                                                  cGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCTGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCa                                                                                                                                                                                                        <  2:59897/250‑1 (MQ=255)
                                                   gCTGGGAATATCCACAAACGGGTTTTATCCCACCGGATCAGTTTTTTCGTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACTCGCCAACTGATGAAACAGACTGCGGATATTCTGATGTCGGTAAAACATTTGCTGCCGGACAATCTCCATATTGGCATCAACGTCTCGGCGGATTGATTTTTTTGAGCGGGGTTTTAACAAGAATGTTCTG‑ACCTGGGTAATAAAATTGGCAAAGATaaaaac                                                                                                                                                                                                         >  2:167615/1‑246 (MQ=255)
                                                        gAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTgg                                                                                                                                                                                                  <  2:50415/250‑1 (MQ=255)
                                                              ccACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTTAAAAAGAGTG‑TCTGAAACTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTtctc                                                                                                                                                                                             >  1:602035/1‑249 (MQ=255)
                                                               cacaAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGa                                                                                                                                                                                           <  2:646119/250‑1 (MQ=255)
                                                                    aCGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTaa                                                                                                                                                                                      <  2:520570/250‑1 (MQ=255)
                                                                         aaTTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTGAAAAAGAAGG‑TCTGAACCCGGGTAATAAATTTGGTAACGGTAAAATCAAGTCGGTTCTCCGGCTAACCGaa                                                                                                                                                                                 >  2:504704/1‑250 (MQ=255)
                                                                            tATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGGCGGGTTTGGAAAAGAGGG‑TTTTAAACTGGTTTATAAATTTGGGAAAGATAAAATTAAACTGGGTCTCAAGCTAACAGaaac                                                                                                                                                                               >  2:366920/1‑247 (MQ=255)
                                                                                ccACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAAcc                                                                                                                                                                         <  1:485754/250‑1 (MQ=255)
                                                                                ccACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTTTTTTTTTGCGGCGGGGGTTTAAAAAGAGGG‑TCTGGAACTGGGTTATAAATTAGGTAAAGATAAAATTCAACTGGTTCTCTCGCTCACGGaaaggaaac                                                                                                                                                                          >  2:304082/1‑243 (MQ=255)
                                                                                 cACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGTTGGCGGGGGTTGTTAAAGAGGG‑TCTGGAGCTTGTGTATAAAATTTGGGACGACAAAAACAAACTTGGTCTCGAGGTTACAGAACGGTAAcc                                                                                                                                                                         >  2:466544/1‑250 (MQ=255)
                                                                                 cACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGTAAAAGAGGG‑TCTGAACCTGGTTTATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAAccc                                                                                                                                                                        >  1:239610/1‑250 (MQ=255)
                                                                                        tCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCg                                                                                                                                                                 <  1:712517/250‑1 (MQ=255)
                                                                                         cAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCATGGGGGTTTGTAAAAGAAGG‑GCTGAAACTGGTTAATAAATTAGGGAACGGTAAAATCAAACTGTGGCTCGGGCGAACCGAAC‑GTCAACCTaattc                                                                                                                                                                  >  2:720292/1‑244 (MQ=255)
                                                                                               agtCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCATCGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCGGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAGCCCTAATCCGGTAACg                                                                                                                                                           <  2:704266/247‑1 (MQ=255)
                                                                                                 tcatctGGCGGAGTCATACGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGGTTTGTAAAAGAGTG‑TCTCGACATTGTTTATAAATTAGGTGACGAAAAAAACCAGGTGGATTTCGAGCGAACGGAAA‑GAAAACCTATTTCTGTGACACCa                                                                                                                                                        >  2:122969/4‑250 (MQ=255)
                                                                                                            aGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTAAAAAGAGTG‑TCTGCACCTCGTTAATCAATTAGGGAACGATAAAAACAAGCTGGTTTTCGCGCTAACGGAAA‑GGCACCCTATTTTCGTAACGTCCAAAGCCAGAGc                                                                                                                                             >  2:712161/1‑250 (MQ=255)
                                                                                                                                  aaTGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGGAGCGGGGTTTGTAAAAAAGGG‑TCTGAACCTGGGTAATAAATTAGGGAACGATAAAATCAAAATGGGTTTCGAGCTAACGGAAC‑GTAACCTCATTCCGGTAAAGCCCAAAAGCAGGGCGCTAATTTATAGCCCTCATCAg                                                                                                                       >  2:18843/1‑250 (MQ=255)
                                                                                                                                   aTGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGc                                                                                                                      <  2:21059/250‑1 (MQ=255)
                                                                                                                                    tGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGca                                                                                                                     <  1:416187/250‑1 (MQ=255)
                                                                                                                                     gACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTGAAAAAGAGTG‑TCTGAACCTGGTTTATAAATTAGGTAACGATAAAAACAAACTGGTTCTTGTGCTTAAGGAAC‑GGAACCCTATTTCGGGAACACCAAAAGCCAGAGCGATGTTTGACAGCCTTCATTACcac                                                                                                                    >  2:429706/1‑250 (MQ=255)
                                                                                                                                         cGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCacaaca                                                                                                                <  2:600862/250‑1 (MQ=255)
                                                                                                                                           ccAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACAtt                                                                                                              <  2:239708/250‑1 (MQ=255)
                                                                                                                                           ccAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAAAGATAAAAATAAGCTGGGTCTCGAGCTAAAGGAAC‑GTAAACCTATTACGGGAACACCAGAAGCCAGAGGGATATTTTACAGCCTTCATCAGCCCAACAt                                                                                                               >  1:120786/1‑249 (MQ=255)
                                                                                                                                            cAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTa                                                                                                             <  1:369384/250‑1 (MQ=255)
                                                                                                                                               cTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACGATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGATTTGTAAAAGAGGG‑GCTGAACCTGGTTAATAAATTAGGGAAAGATAAAATAAAGCTGGTTCTCGAGCTAACGGAAC‑GTAAACCCATTCCCGTAACGCCAGAAGACAGAGAGATATTTTAAAGCCTTCATCAGCACAAAATTACCt                                                                                                          >  2:10615/1‑250 (MQ=255)
                                                                                                                                                   tgaAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTgc                                                                                                      <  1:690132/250‑1 (MQ=255)
                                                                                                                                                           aCTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGa                                                                                                                                                      <  1:645045/194‑1 (MQ=255)
                                                                                                                                                                  atatTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCCGCGGGGTTTTAAAAAAAGGG‑TCTGAACCTGGGTAATAAATTAGGTAACGATAAAATCAAGGTGGGTTTCGAGGTAACGGAACGGTAACCCT‑TTCCGGGAAAGACACAAAGCCGAGCGATATTTTACCAGCTTTATCACCACAACCATACCATTGCGCTGGGTGGATTTgg                                                                                       >  2:150096/1‑250 (MQ=255)
                                                                                                                                                                    atTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTTAAAAGAGTG‑TCTGAACCTGGTTTATAAATTAGGTAACGATAAAATAAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGAAAGCCCTCATCAGCTCAACATTACCTTTGCGCTGGATGACTTTGTTa                                                                                     >  2:131532/1‑250 (MQ=255)
                                                                                                                                                                        tGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTTCGCTGGATGACTTTGGTACggg                                                                                 >  1:291060/1‑250 (MQ=255)
                                                                                                                                                                          aTGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGtt                                                                               <  1:563840/250‑1 (MQ=255)
                                                                                                                                                                              cGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCTGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGc                                                                           <  2:510761/250‑1 (MQ=255)
                                                                                                                                                                              cGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGGAAAGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAAACCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTTACAGCCTTCATCAGCACAACATTAACTTTGCGCTGGATGACTTTGGTACGGGGTATGc                                                                           >  1:66182/1‑250 (MQ=255)
                                                                                                                                                                                      cATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTTTAAAAGAGTG‑GCTGAACCTGGTTTATTAATTAGGTAACGATAAAAACAAGCTGGTTCTCGAGCTAAAGGAAC‑GTAAACCCATTTCGGTAAACCCAGAAGCCAGAGCGGTATTTTACAGCCTTCATCAGCACAACATTACCTTTTCGCTGGATGACTTTTGTACGGGGTATGCGACCTATc                                                                   >  2:673106/1‑250 (MQ=255)
                                                                                                                                                                                                   aCAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTGAAAAGAGTG‑TCTTCACCTTGGTAATTAAATAAGTAACGAAAAAATCAAGCCGGTTCTCGAGCTAACGGAAC‑GTAAACCTATTTCGGGAAAACCCGAAACCAGAGCGAAATTTGACAACCTTCATCCGCACAACATTACCTTTGCGCTGGCTGGCTTTTGTACGGGGTATGCGACCTATCCTTAccttccggc                                                      >  2:336734/1‑242 (MQ=255)
                                                                                                                                                                                                   aCAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGc                                                      <  2:365179/250‑1 (MQ=255)
                                                                                                                                                                                                          ccATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGGAGCGGGGTTTGAAAAAGAGTG‑GCTGGAACCGGTTAATAAATTAGGGAACGATAAAAACAAGCTGGTTTTCGAGCTAAAGGAAC‑GTAACCCTATTTCGGGAACGCCAAAAGCCAGAGCGGTATTTTACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGGCTTTGGGACGGGGTATGGGACCTATTGTTAATTTCCGGCGTTCCCg                                               >  2:660276/1‑250 (MQ=255)
                                                                                                                                                                                                               ttGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCg                                           <  2:712925/249‑1 (MQ=255)
                                                                                                                                                                                                                  gCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGAtt                                        <  2:203950/249‑1 (MQ=255)
                                                                                                                                                                                                                               ggcggGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGa                           <  1:8876/249‑1 (MQ=255)
                                                                                                                                                                                                                                  gggTTGGTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGTCGTTCCCGGTCGATTTTATTAAGATCGATAAg                       <  1:452847/250‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGt                      <  2:602035/250‑1 (MQ=255)
                                                                                                                                                                                                                                          ttttGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTtgt               >  1:236229/1‑250 (MQ=255)
                                                                                                                                                                                                                                             tGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGc             <  2:659726/249‑1 (MQ=255)
                                                                                                                                                                                                                                              ggCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCa            <  2:44737/249‑1 (MQ=255)
                                                                                                                                                                                                                                               gCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCaa           <  2:94417/249‑1 (MQ=255)
                                                                                                                                                                                                                                                cAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAAt         <  2:428977/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 aGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATg        <  2:422465/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       aTTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGAgt  <  1:106626/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
CGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTG‑TCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAAC‑GTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGAGT  >  NZ_CP009273/328464‑328960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑