| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,032 | 0 | T | G | 36.1% | 112.9 / 21.2 | 61 | V288G (GTG→GGG) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base T (2/37); new base G (22/0); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTC > NZ_CP009273/594783‑595275 | aaGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt < 1:558526/250‑1 (MQ=255) aaaaGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGgtggtg < 1:369311/249‑1 (MQ=255) cgggATGCGTGAAACCAGGAAGCGCGCGGTTCGTCGATCGAACTGGGGGAAGCTGAATATATGGTGCGCGCGAGCTGCTATCTGCAAACGCTCGACGACTTTAATCACAGCGTGTTAAAAGCCGGTAAAAAAGGCGTGCCCGCTTATCTGCGCGATGTTGCGAAGGTCCAGATTGACCCGGTGATGCGCCGGGGCATTACCGAACTAAACGGCGGAGGCGAGGTGGCGGGCGGGGTGGTGATCCGGCGAt < 2:388079/248‑1 (MQ=255) ttCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGATCCTGCGATCCGGCaa > 1:115749/1‑249 (MQ=255) cAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaa < 2:203601/249‑1 (MQ=255) aaCCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACg < 1:734177/250‑1 (MQ=255) ccAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGcc < 1:14890/250‑1 (MQ=255) cAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGGTCCGGCCAAAAAGccc > 1:64467/1‑250 (MQ=255) aGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGaa < 2:497787/250‑1 (MQ=255) gggcggTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTGCTGCGATTCGGCAAAAAAGACCGAGAAGTg > 1:584642/1‑250 (MQ=255) gggcggTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGATCTGCGGTTCGGCCAAAAAGCCCGGGAAggg > 1:739578/1‑248 (MQ=255) gggcggTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGGGCCGGCCAAAAAGCCCCCGAAggg > 1:456815/1‑248 (MQ=255) ggcggTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGa < 2:286457/250‑1 (MQ=255) gTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgc < 1:167499/250‑1 (MQ=255) cgtcgATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg < 1:403470/249‑1 (MQ=255) cgatcgaACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGt > 1:657360/1‑250 (MQ=255) atcgaACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGAGCCCGCAAAAACGCCCGAGAAGGGATCgccg‑ccct > 1:233080/1‑246 (MQ=255) tcgaACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGaa < 1:389725/250‑1 (MQ=255) aaCTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGGCTGCGATCCGGCGAAAAAACCCGCGGAGGGGTCggcg‑cccgggaggg > 2:662592/1‑242 (MQ=255) cTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑a < 1:445130/250‑1 (MQ=255) cTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGGCCCGGCAAAAACGCCCGCGAAGTGGTCgccg‑cccgggaggga > 1:593769/1‑240 (MQ=255) tGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGTCCGGCAAAAAACCCCGGGGAGTGATCGCCG‑CCGTGAAGGAC‑a > 1:582779/1‑249 (MQ=255) gaagcggaaTATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTgg < 1:289381/250‑1 (MQ=255) agcggaaTATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑aa < 1:435212/250‑1 (MQ=255) gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGGAGGCGAAGTGGCGGGCGGGGGGGTGGACCTGCGATCCGGCACAAAAGCCCGAGGAGTGAACGCCC‑CCCTGGAGGAC‑AAACATG‑AAACGCCGgaaaaaagt > 1:243325/1‑245 (MQ=255) gcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTTCGGTCCGGCGAAAAAGACCCGGGAGGGAGCGACG‑CCGGGGAGGAG‑AAACTGGAAAACGCGGaaagaagtgtgc > 2:456599/1‑241 (MQ=255) cGGATATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGaa < 1:639835/250‑1 (MQ=255) aggtgatcaagaaaCGCTCGACGCCTTGAATCGCATCGTTTAAAAAGCAATTGAAAAGGACGTGCCCGTTTATCTGCTCGATGTTGCGAAGGTCCAGATGGGCCCGGAGATGCGCGGGGACATAGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAGCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGA‑AAACGGTGAAAAGTAGTCT‑GCCGGaa < 2:318093/239‑1 (MQ=255) tCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCg < 1:401451/248‑1 (MQ=255) gCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGgag < 2:123972/250‑1 (MQ=255) cAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTg < 2:535666/246‑1 (MQ=255) aCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAg < 2:80860/250‑1 (MQ=255) gtcTTTAATCTCACCGTTTTAAAGGCCAGTGAAATTGGCTTGCCCGTTTATCTGCGCTATGTTGCGTAGGTCCAGATTGGCCCGGAGATGCGCCGTGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTacaaca < 2:312234/247‑1 (MQ=255) cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCCGCAAAAACGCCCCGGAAGTGGTCGCCG‑CCGGGAAGGGC‑AAACGGG‑AAACGCCGGAAAGAAGTAT‑GCCGGGAGGGGGGGGGGTAGTTtcaaaaa > 2:161781/1‑245 (MQ=255) aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCg < 2:742127/250‑1 (MQ=255) acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGcag < 1:186322/250‑1 (MQ=255) aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCCGACCGGCCAAAAAGCCCCAGGAGGGATCGGCGCCCCGGAGGGAA‑AAACGGG‑AAA‑GCGGAAAAGAAAGGT‑GCCCGGAGGGGGGGGGGGAGGTTAAACAT‑ACGATCGcagcc > 2:709863/1‑250 (MQ=255) ttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTc < 1:193788/249‑1 (MQ=255) ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACcg < 1:329876/250‑1 (MQ=255) cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGAGGGGGGGGGAGCCTGCGCTCCGGCGAAAACGCCCCGGAAGGGATCGCCC‑CCGCGGAGGAA‑AAAATGG‑AAAAGCCGAAAAGTAGACT‑GCCGGGAGGCGTGGGGAGAGTTACAACAT‑ACGAACGCAGACAGCCCA‑TTTACCGCGCCCGCGAC‑AACCTc > 2:641681/1‑249 (MQ=255) cGTTTATCTGCGCGATGGGGCGAAGGTGCAGATGGGGCCGGCGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCTAATGGGGGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGACGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGc < 2:718003/250‑1 (MQ=255) gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCt < 1:641681/250‑1 (MQ=255) cgctGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGAGCCTGCCGCCCGGCAAAAACGGCCCGGACGGGGTCCGCG‑CCCTGGAGGAC‑AAACTGG‑GAACCCTCACACGGAATGC‑GGCCGAAGGGGGGGCGGTCGTTACAAAAC‑ACGGTCGCAGTCAGCCCC‑ATCAACGCGCCCTCCAC‑ACACTCCCCGGCACGGTGCTg > 2:168393/4‑250 (MQ=255) aGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTAt < 1:136312/250‑1 (MQ=255) gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTCCGGTCCGGCCAAAACGCCCCAGCAGTGGGCGCCC‑CCGGGAAGGGC‑AAACAGG‑AAACACCGGAAAGGAGTCTGGCGGGAGGGGGGGGGGATA‑TTACAACATAACGA‑CGCAGCCACCTCACTTACCCCCGCCTCCGCC‑AACCTCCGCGGC‑AGTTGGCGGAAGCAGTTATtg > 2:255479/1‑250 (MQ=255) tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTCCGGTCCGGCAAAAAAGCCCGAGAAGGGGACGCCG‑CCGCGAAGGACAAAATTGG‑AAACGCGGAAAAG‑AGTCT‑GCCCGGAGGGGTGGGGAAAATTACAACAA‑ACGATAGCAGCCCACTCC‑TTGACCGCGCCCTCGGC‑CACCTCAGCGGCCAGGTGCGGGGAGAGGTTTTtgt > 2:609653/1‑250 (MQ=255) cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAAAGCCCGGGAAGGGAGCGCCG‑CCCGGAAGGAC‑AAACTTG‑GAACGCTGGAAAGGAGTCT‑GGCGGCAGGCGTGGGGATAGTTTAAACAA‑ACGATGGCAACCACCTCA‑TTATACGCGCCATCAGC‑AACCACACACGGAAGGTGCTTGACGAGTTTTTTgtgg > 2:538683/1‑250 (MQ=255) aGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGGGATCCGGCCAAAAAGCCCGAGAAGGGGACGCCG‑CCCGGGAGGAG‑AAACGGG‑AAACGACGAAAAGGAGGCT‑TCCCGGAGGGGTGGAGATAGTTACAAAAA‑ACGATCGCAGCCCGCCCC‑TTGTCAGGGCCATCGACAAACCTCACGGGCACGTTG‑TGGTAGGGGTTAtttgg > 2:327010/1‑245 (MQ=255) gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTgtggtg < 1:571474/250‑1 (MQ=255) gATGGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTgtggtg < 2:319800/250‑1 (MQ=255) ccGGAGAGGCGCCGGGGCATGGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAg < 1:557553/250‑1 (MQ=255) gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCt < 2:305746/250‑1 (MQ=255) ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGCAAAAAAGCCCGAGAAGTGGTCGCCG‑CCGTGAAGGAC‑AAAATGG‑AAAAGCTGAAAAATAGACT‑GCCGGGAGGCGGGGAGAGAGTTAAAACAT‑ACGATCGCAGCCAGCCCC‑TTTACCGCGCCACCGAC‑CACCTCAGCGGCGAGGTGCTGGGAGGGTTTAATGTGGTGGTGGTGGGCtgcgggcct > 2:440084/1‑243 (MQ=255) cATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCCAAAACGCCCGAGAAGTGATCGCCG‑CCGGGAAGGAC‑AAACTGG‑AAAAGCTGAAAAGTAGACT‑GCCGGAAGGCGTGGGGGTAGTTACAAAAT‑ACGATCGCAGCCAGCTCC‑TTGACCGCGCCACCGAC‑AACCTCAGCGGCGAGTTGCTGGGAGAGGTTATTGTGGTGGCGGGTGTCGGTgggccggtgttc > 2:143532/1‑242 (MQ=255) cGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGGGGTCATAAGTGCCGGGAAAAAAAACCCAGAAGGGATCGACC‑CCGCGAAGGGC‑AAACTCG‑GAAAGCAGGAAAGAAGTGA‑TACGGAAGGGGGGGGGGAAGTTACAAAAT‑ACCATCGCCGCCAGCTCA‑TTGACCCCGCCACCGAC‑AACCCAAACGGCACAGTGCTGGGCGAGTCTTCTGTGGTGGCGGGATGCGGTGTGCTGGTTCTCCCGCAt > 2:658181/1‑250 (MQ=255) gcggaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGgc < 2:243325/250‑1 (MQ=255) gcgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGAGCGCAGCCAGCTCA‑ATGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGgc < 2:611703/250‑1 (MQ=255) aaggcggagTGGCGGGCGGGGTGGTGATCCGTCGTACCGGAAAAAACGCCCGAGAAGGGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCt < 2:12477/250‑1 (MQ=255) gaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCtggtgg < 1:310591/250‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGattat < 2:398091/250‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGattat < 1:532206/250‑1 (MQ=255) gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGattatt > 1:545490/1‑250 (MQ=255) gcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTc < 1:470764/250‑1 (MQ=255) | AAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCG‑CCGTGAAGGAC‑AAACTGG‑AAACGCTGAAAAGTAGTCT‑GCCGGAAGGCGTGGAGATAGTTACAACAT‑ACGATCGCAGCCAGCTCA‑TTGACCGCGCCATCGAC‑AACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTC > NZ_CP009273/594783‑595275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |