Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,236,683	A→C	M282R (ATG→AGG)	cvrA ←	potassium/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,236,683	0	A	C	93.0%	181.0 / 6.5	57	M282R (ATG→AGG)	cvrA	potassium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base C (25/28);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

AACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCC  >  NZ_CP009273/1236437‑1236918                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            aaCAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTgg                                                                                                                                                                                                                                          <  1:360380/250‑1 (MQ=255)   cAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGc                                                                                                                                                                                                                                         >  2:165517/1‑249 (MQ=255)         tGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTgc                                                                                                                                                                                                                                  >  2:316186/1‑250 (MQ=255)          gCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGAAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTgcg                                                                                                                                                                                                                                 >  1:722348/1‑250 (MQ=255)            aTTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTgcgc                                                                                                                                                                                                                                >  1:617530/1‑249 (MQ=255)                tcCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCgccagc                                                                                                                                                                                                                            >  2:363887/1‑249 (MQ=255)                    gCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGc                                                                                                                                                                                                                       <  2:96251/250‑1 (MQ=255)                    gCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGc                                                                                                                                                                                                                       >  2:404073/1‑250 (MQ=255)                       ccGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAg                                                                                                                                                                                                                    >  1:156938/1‑250 (MQ=255)                               atcGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGa                                                                                                                                                                                                            <  1:531809/250‑1 (MQ=255)                                tcGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGaa                                                                                                                                                                                                           <  1:254729/250‑1 (MQ=255)                                tcGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGaa                                                                                                                                                                                                           <  2:529937/250‑1 (MQ=255)                                tcGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGaa                                                                                                                                                                                                           <  1:518397/250‑1 (MQ=255)                                  gggAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGaaa                                                                                                                                                                                                          <  1:473725/249‑1 (MQ=255)                                  gggAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAAt                                                                                                                                                                                                         >  2:471401/1‑250 (MQ=255)                                         cGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAg                                                                                                                                                                                                  >  1:571549/1‑250 (MQ=255)                                            ccAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGAt                                                                                                                                                                                               <  2:284357/250‑1 (MQ=255)                                                         cACCGCGCCGCGTAATCCTCCCCAGCTGATAAACACGCGCCCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGAACGGGCGAAGAACATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAACAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGCAACTTTGCAGGATGCCGTAGCGGATg                                                                                                                                                                                  >  2:124687/1‑250 (MQ=255)                                                              caccgcgCCATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTGGCAGGATGCCGTAGCGGTTGCGAAt                                                                                                                                                                             <  2:341446/248‑1 (MQ=255)                                                                       aTCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGAtt                                                                                                                                                                    <  1:651872/250‑1 (MQ=255)                                                                             cccAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCca                                                                                                                                                               <  1:338574/249‑1 (MQ=255)                                                                                    gATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGaaaa                                                                                                                                                       >  1:568077/1‑250 (MQ=255)                                                                                              cgcTCACGCAGATTGAAACCGCGGCAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGa                                                                                                                                              >  2:3927/1‑249 (MQ=255)                                                                                                         aTTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGa                                                                                                                                  <  2:705695/250‑1 (MQ=255)                                                                                                              aaCCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATAcc                                                                                                                             >  2:74290/1‑250 (MQ=255)                                                                                                              aaCCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATAcc                                                                                                                             >  1:534171/1‑250 (MQ=255)                                                                                                                  gcgGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTa                                                                                                                         >  2:444045/1‑250 (MQ=255)                                                                                                                    ggAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTAcc                                                                                                                       >  1:83634/1‑250 (MQ=255)                                                                                                                               aaTCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGCTTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGGTTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCATCCAGCGAag                                                                                                            >  2:292836/1‑250 (MQ=255)                                                                                                                                aTCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCagt                                                                                                           <  1:312918/250‑1 (MQ=255)                                                                                                                                 tCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCAGCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCagta                                                                                                          <  2:14839/250‑1 (MQ=255)                                                                                                                                         aTACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATga                                                                                                  <  1:743311/250‑1 (MQ=255)                                                                                                                                            ccGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATgaga                                                                                                <  1:589786/249‑1 (MQ=255)                                                                                                                                            acGAGAGGGAACGGGGCAAGAATATCATCCTTGCGTACAAAAAGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTATCCATCAGCCCCAGCACCATGAGCCGGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGGATAGGGCGATTGCCCAGCAGAAAACCGCAAAGATACACCGGCAGAATACCACTACCTTCCAGCGCAGTAGTTAATGAGaa                                                                                               <  2:639103/249‑1 (MQ=255)                                                                                                                                                      aCGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATa                                                                                     <  1:580014/250‑1 (MQ=255)                                                                                                                                                             aagaaTATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGCAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTCATGAGTAAATCAGAATACCGCAAc                                                                              >  2:188318/1‑250 (MQ=255)                                                                                                                                                                aaTATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACtt                                                                            >  1:704111/1‑249 (MQ=255)                                                                                                                                                                      atcCATGCGGACAAAATGGGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCACCAGCCCCAGCGCCAGAAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAAAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGcc                                                                      <  1:208416/249‑1 (MQ=255)                                                                                                                                                                       tcCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAg                                                                    <  1:363887/250‑1 (MQ=255)                                                                                                                                                                        ccATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAg                                                                    >  2:734336/1‑249 (MQ=255)                                                                                                                                                                                    aaTGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAACCCGCACAGATACCCCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAAt                                                       <  1:650074/250‑1 (MQ=255)                                                                                                                                                                                          cgcCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCcggcg                                                 <  1:329558/250‑1 (MQ=255)                                                                                                                                                                                          cgcCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCcggcg                                                 <  1:220472/250‑1 (MQ=255)                                                                                                                                                                                             cGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCggg                                               >  2:201655/1‑249 (MQ=255)                                                                                                                                                                                                aaTGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAgg                                           <  1:635834/250‑1 (MQ=255)                                                                                                                                                                                                 aTGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAggg                                          >  2:96362/1‑250 (MQ=255)                                                                                                                                                                                                    acAAAAGGCAGCAGCCCCCTTGGGTAACCCAACCGCCCCAGCACCAGGACCCGGGCGATTTGCGCCGGCCGGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTGGCGGTTGCAAATAGGGCGATTCCCCAGCAGAAACCCGCACAGATACACCGCCAGAATCCCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGa                                       <  2:419205/249‑1 (MQ=255)                                                                                                                                                                                                        tAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCg                                   >  2:687589/1‑250 (MQ=255)                                                                                                                                                                                                                 gATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCAt                          >  1:142469/1‑250 (MQ=255)                                                                                                                                                                                                                      cTTGGGTTAACCACCAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCa                     <  1:77266/250‑1 (MQ=255)                                                                                                                                                                                                                           gTTAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCagtagt                <  1:404073/250‑1 (MQ=255)                                                                                                                                                                                                                            ttACCCAACACCCCCAGCACCAGAAACCTGGCGATTTGCGCCAGCCGGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTCCCCAGCAGAAAACCGCAAAGATACCCCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAAAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAgtagta               <  1:316186/250‑1 (MQ=255)                                                                                                                                                                                                                             tAACCAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGTCAGCCAGGCGAGGCCGTCGAAATCTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCTGGCAGGGCGATGCGGTTGATCATTTGCAgtagta               >  2:140606/1‑249 (MQ=255)                                                                                                                                                                                                                                 cAACAGCCCCAGCACCAGGAACCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGCAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAATTaa          >  2:697042/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaCAGCCCCAGCACCAGGACCCTGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAAc         <  1:150321/250‑1 (MQ=255)                                                                                                                                                                                                                                      gCCCCAGCACCAGGAACCGGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGAGGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAAccgcc     <  1:152187/250‑1 (MQ=255)                                                                                                                                                                                                                                          cAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCgccgcc  >  2:81477/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            AACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGAATATCATCCATGCGGACAAAATGAGCGCCGGAATGGCAATAGGCAGCAGATCGCTTGGGTTAACCAACAGCCCCAGCACCAGGAACATGGCGATTTGCGCCAGCCAGGCGAGGCCGTCGAAATTTTGCAGGATGCCGTAGCGGTTGCGAATAGGGCGATTACCCAGCAGAAAACCGCACAGATACACCGCCAGAATACCGCTACCTTCCAGCGCAGTAGTTAATGAGAAAATCAGAATACCGCCACTTAATGCCAGCAATGGATATAATCCGGCGGGCAGGGCGATGCGGTTGATCATTTGCAGTAGTAAATAACCGCCGCC  >  NZ_CP009273/1236437‑1236918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑