Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,330,552	G→A	Q22* (CAG→TAG)	mlaB ←	lipid asymmetry maintenance protein MlaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,330,552	0	G	A	100.0%	327.4 / NA	87	Q22* (CAG→TAG)	mlaB	lipid asymmetry maintenance protein MlaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/40);  total (47/40)

TCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCAC  >  NZ_CP009273/3330306‑3330798                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATc                                                                                                                                                                                                                                                     <  2:538516/250‑1 (MQ=255)       cTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGctc                                                                                                                                                                                                                                               <  2:53129/250‑1 (MQ=255)         gAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCtctc                                                                                                                                                                                                                                             >  2:527234/1‑250 (MQ=255)          aaaaaaTTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGctct                                                                                                                                                                                                                                              <  1:110893/248‑1 (MQ=255)               aTTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGAt                                                                                                                                                                                                                                        >  2:407053/1‑249 (MQ=255)                    aCGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGc                                                                                                                                                                                                                                   >  2:464985/1‑249 (MQ=255)                     cGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGc                                                                                                                                                                                                                                   >  1:625568/1‑248 (MQ=255)                       aGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGc                                                                                                                                                                                                                               <  2:361544/250‑1 (MQ=255)                           aGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCACCTCTCCGGATAACTCCAGCgggt                                                                                                                                                                                                                           >  2:680049/1‑250 (MQ=255)                               cATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCAcc                                                                                                                                                                                                                       >  1:429238/1‑250 (MQ=255)                                 tCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCg                                                                                                                                                                                                                     <  1:236156/250‑1 (MQ=255)                                              tataCAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCt                                                                                                                                                                                                        >  1:266047/1‑250 (MQ=255)                                                 aCAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGTGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAg                                                                                                                                                                                                     >  1:517746/1‑250 (MQ=255)                                                  cAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGt                                                                                                                                                                                                    >  1:357730/1‑250 (MQ=255)                                                         gCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCg                                                                                                                                                                                              <  1:574806/249‑1 (MQ=255)                                                          ccAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCt                                                                                                                                                                                            >  1:159679/1‑250 (MQ=255)                                                          ccAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCt                                                                                                                                                                                            <  1:154913/250‑1 (MQ=255)                                                           cAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTCTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTc                                                                                                                                                                                           <  1:118794/250‑1 (MQ=255)                                                              ggTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCatt                                                                                                                                                                                        <  2:695495/250‑1 (MQ=255)                                                               gTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCatta                                                                                                                                                                                       >  1:736619/1‑250 (MQ=255)                                                                tataCACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCattat                                                                                                                                                                                      <  1:661578/250‑1 (MQ=255)                                                                  taCACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCattatt                                                                                                                                                                                     <  2:358083/249‑1 (MQ=255)                                                                    tacTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGCCACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCACTGACTCCCTCATTCttttt                                                                                                                                                                                  >  2:408068/2‑250 (MQ=255)                                                                      cTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTtc                                                                                                                                                                                >  2:1862/1‑250 (MQ=255)                                                                        ttGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTtctc                                                                                                                                                                              >  1:154756/1‑250 (MQ=255)                                                                           tCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGAATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTc                                                                                                                                                                           >  2:515109/1‑250 (MQ=255)                                                                               ttACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCa                                                                                                                                                                         >  2:593108/1‑248 (MQ=255)                                                                                 aCCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGt                                                                                                                                                                     >  2:153889/1‑250 (MQ=255)                                                                                     ccTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGAtt                                                                                                                                                                 >  1:117253/1‑250 (MQ=255)                                                                                       tGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCCGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAACGCGGTAGCAAAACGTCCTAATCAAGATCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTCCCAGAGTGAtttt                                                                                                                                                               >  2:494381/1‑250 (MQ=255)                                                                                         aaGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCt                                                                                                                                                             >  1:446775/1‑250 (MQ=255)                                                                                                acaTTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTgaga                                                                                                                                                      <  1:502536/250‑1 (MQ=255)                                                                                                 caTTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGaa                                                                                                                                                     <  2:539891/250‑1 (MQ=255)                                                                                                  aTTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCTCCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGaaa                                                                                                                                                    >  2:20525/1‑250 (MQ=255)                                                                                                  aTTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGaaa                                                                                                                                                    >  1:429750/1‑250 (MQ=255)                                                                                                    tgttgCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATc                                                                                                                                                  >  2:194169/1‑250 (MQ=255)                                                                                                     gttgCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATc                                                                                                                                                  <  2:596729/249‑1 (MQ=255)                                                                                                      ttgCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCg                                                                                                                                                 <  2:113263/249‑1 (MQ=255)                                                                                                          ccTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTc                                                                                                                                            <  1:667781/250‑1 (MQ=255)                                                                                                           cTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCa                                                                                                                                           <  2:616372/250‑1 (MQ=255)                                                                                                            tGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAg                                                                                                                                          >  2:745702/1‑250 (MQ=255)                                                                                                               tttttCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTg                                                                                                                                       >  2:425931/1‑250 (MQ=255)                                                                                                                ttttCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTgc                                                                                                                                      >  1:644388/1‑250 (MQ=255)                                                                                                                 tttCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTg                                                                                                                                       <  1:633628/248‑1 (MQ=255)                                                                                                                  ttCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTgcg                                                                                                                                     <  1:516803/249‑1 (MQ=255)                                                                                                                   tCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTgcgc                                                                                                                                    <  2:223437/249‑1 (MQ=255)                                                                                                                       cAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCa                                                                                                                               <  2:516440/250‑1 (MQ=255)                                                                                                                         gATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAgg                                                                                                                             >  1:674266/1‑250 (MQ=255)                                                                                                                           tCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGcc                                                                                                                           >  1:665901/1‑250 (MQ=255)                                                                                                                               tAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTc                                                                                                                        >  1:306384/1‑249 (MQ=255)                                                                                                                                    tGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATAcc                                                                                                                  <  1:412989/250‑1 (MQ=255)                                                                                                                                             agTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGACATCGATTTCAGTTGCGCAGTCAGGCCGTCGCTACCTTTGGTACg                                                                                                         >  2:707313/1‑250 (MQ=255)                                                                                                                                                 ccAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACgcagc                                                                                                     <  2:392456/250‑1 (MQ=255)                                                                                                                                                    gTCCCCCCGTATCCACGCGGGAGACACAGCTAAGATCGATGCAGGTAATCCCCGTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCagcagc                                                                                                  <  1:390948/250‑1 (MQ=255)                                                                                                                                                       cccccGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGtt                                                                                               >  1:721596/1‑250 (MQ=255)                                                                                                                                                        cgccGTAGCCACGCTGGAGACACCGCTAAGAACCATGCAGGTAATCCCCTTCTCCGCTTCCTCACGCCTTGCCCAAAGTGGTAGCAAAACGTCCTAATCCAGCTCTCCAGATAACGCCAGCGTGTCACCCGTCTGCGTCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCATAGTGATTTTCTGTTGAGAAATCGATTTCAGTTTCGCTGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTc                                                                                              <  2:355071/248‑1 (MQ=255)                                                                                                                                                          ccGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTccc                                                                                            <  1:523469/250‑1 (MQ=255)                                                                                                                                                            gTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCa                                                                                          <  2:341326/250‑1 (MQ=255)                                                                                                                                                              aTCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACt                                                                                        >  1:366134/1‑250 (MQ=255)                                                                                                                                                                    gcgGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGtttt                                                                                  <  2:198753/250‑1 (MQ=255)                                                                                                                                                                         agaCACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTtttgtttg                                                                             >  1:445136/1‑250 (MQ=255)                                                                                                                                                                          gaCACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTtgg                                                                            <  1:557437/250‑1 (MQ=255)                                                                                                                                                                            cacGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTtggtg                                                                          <  1:123016/250‑1 (MQ=255)                                                                                                                                                                                 cTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGAt                                                                     >  1:51153/1‑250 (MQ=255)                                                                                                                                                                                          aTGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGAc                                                            <  2:539986/250‑1 (MQ=255)                                                                                                                                                                                           tGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACg                                                           >  2:640876/1‑250 (MQ=255)                                                                                                                                                                                             cAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGcc                                                         <  2:518728/250‑1 (MQ=255)                                                                                                                                                                                               ggTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCtt                                                       <  2:149715/250‑1 (MQ=255)                                                                                                                                                                                                 tAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCa                                                     <  2:545225/250‑1 (MQ=255)                                                                                                                                                                                                     ccccTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCaa                                                 >  2:64729/1‑250 (MQ=255)                                                                                                                                                                                                        catCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCa                                              <  1:573579/248‑1 (MQ=255)                                                                                                                                                                                                         ttCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATACAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCAt                                             >  2:458534/1‑250 (MQ=255)                                                                                                                                                                                                           cACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGTCACGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGGCGATGCCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCAt                                             <  1:679029/248‑1 (MQ=255)                                                                                                                                                                                                                cgtCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCCCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACGCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTaa                                      <  2:703437/248‑1 (MQ=255)                                                                                                                                                                                                                cctCCTCACGCATTTCCCAATCTGTTAGCAAAACGTTCTAATCCAGCTCTCCGGATAACGCCAGCGTGTTACCCGTCTGCACCCACCTCACTGACTCGCTCATTCTTTTCTCTCTTCCATAGTGATTTTCTGTTGTGAACTCGACTTCAGCCCTTCAGTCGGGCCGTCAATACCTTTGGCACGCACCACCGTTCCCCACTCGTTTTGTGTGGTGGTGATCTCACTGACGCCTTCAGCAATCATGTCGTaa                                      >  2:523608/3‑250 (MQ=255)                                                                                                                                                                                                                 ttCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAg                                     >  1:13738/1‑250 (MQ=255)                                                                                                                                                                                                                   ccTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCCTACTGACGCCTTCAGCAATCATGTCGTAAGcc                                   >  2:658071/1‑250 (MQ=255)                                                                                                                                                                                                                        cGCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGcc                               >  1:6789/1‑249 (MQ=255)                                                                                                                                                                                                                         gCATTTCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACACCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCaa                             <  1:366271/250‑1 (MQ=255)                                                                                                                                                                                                                              tCCCAAAGCGGTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGc                         <  1:99636/249‑1 (MQ=255)                                                                                                                                                                                                                                       ggTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTgg                 >  2:740528/1‑248 (MQ=255)                                                                                                                                                                                                                                       ggTAGCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGa               <  2:266047/250‑1 (MQ=255)                                                                                                                                                                                                                                           gCAAAACGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGttt           >  2:579543/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaCGTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTAc       >  2:181123/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gTCCTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGc     >  2:138369/1‑249 (MQ=255)                                                                                                                                                                                                                                                    ccTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCAc  >  1:478340/1‑250 (MQ=255)                                                                                                                                                                                                                                                    ccTAATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATAATGTCGTAAGCTTGCCAATTGCCCGTCTGGGAGTTTTTACGCCAc  >  2:610337/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TCCGGCCTGAAAAAATTTAACGAGGCAGAACATCAGCAGGCAAATTATACAATTTCGCCAGGGTATACACTTTGTCGTTTACCCCCTGAAGCGTCACATTGTTGCCCTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCAC  >  NZ_CP009273/3330306‑3330798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑