| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 37.1% | 117.3 / 22.2 | 70 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (9/35); new base G (26/0); total (35/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAG > NZ_CP009273/2089326‑2089816 | tGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGtt > 1:707031/1‑248 (MQ=255) tGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTa > 2:2432/1‑246 (MQ=255) gCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtctc < 1:492692/250‑1 (MQ=255) cATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGAt > 1:405133/1‑250 (MQ=255) cATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAGGGCTCTGGGAGATG‑Ccccgc > 2:955929/1‑248 (MQ=255) tGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGaaaa < 2:342823/250‑1 (MQ=255) ctcGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGGGGAGGGGGGGAGTAAGGGTCGGGGAGAGGACGGCGAAAAta > 2:725782/1‑249 (MQ=255) gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTc < 1:495407/247‑1 (MQ=255) gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCtt < 2:628222/249‑1 (MQ=255) gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCAGGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCACGAAAATTCt < 2:34696/248‑1 (MQ=255) cGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTATGCATCTTGGAGATG‑CCCCCGAAATTCctttc > 2:929412/1‑248 (MQ=255) gTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTg < 2:970649/249‑1 (MQ=255) tGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTTAGTCGCCGGGAGATG‑CCGCGACAATTTTTTCCTTTTgc > 2:366902/1‑250 (MQ=255) ggTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCTGGAAGATG‑CCCCGCAAATTCTTTCCTTTGGCGCgg > 1:19046/1‑250 (MQ=255) tCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGAt < 2:86477/250‑1 (MQ=255) aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATaa < 1:851055/250‑1 (MQ=255) gtggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATaaaa < 2:207312/250‑1 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACtctc < 1:94414/250‑1 (MQ=255) aGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTcc < 1:531024/250‑1 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTgc < 2:486105/249‑1 (MQ=255) cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTg < 1:431883/250‑1 (MQ=255) cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTg < 1:304037/250‑1 (MQ=255) cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTTCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTGCG‑CTg > 1:278930/1‑250 (MQ=255) ccATGAAATCATTGGCGATATCGTGCCGCTGGAAAAACGCTACGCTGAGGAAGGCGCTGACGAAATGGTGTTCTACGATATCACCGTTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCGTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCa < 2:374001/250‑1 (MQ=255) aTGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATG‑CCGCGGAAAATCTTTTCTTTTGCGCGGGGAAAATTTCCCTCAAACCTCCCGCG‑CTGGCaaa > 1:709315/1‑248 (MQ=255) gAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGGAGATG‑GCGCGAAAATTCTTTCCCTTTGCGCCGGTAAAAATTCCCTCAACCCTTCCGCG‑CTGGCCG‑Acc > 1:188705/1‑250 (MQ=255) aTCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTTCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTTCG‑CTGGCAG‑ACCCa > 1:350040/1‑249 (MQ=255) ttGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACatt < 1:508653/250‑1 (MQ=255) ttGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCCGGAAGAAG‑GCCCGAAAAATTTTTTCCTTGGCGCGGGTAAAAATTTCCTCAACTCTCCCGCG‑CTGGCGC‑ACCCCACat > 2:270896/1‑249 (MQ=255) tggagttaccGTGCCGCGGACAAAACGCGACGGTGAAGAAGGGGCTGACGAACTGGTATTCTTCGATATCACCGTTGCCAGCGGTGGCCGTGTGGTAGATAAAAGCTGGGTGTCTCGCGTGGCGGAAGTGATCGACATTCCGTTGTGTGTGGCGGGTGGGATTAAGGCTCTGGAAGATG‑CCGCGAAAATTCTTTCCGATGGCGCGGATAAAATTTCCATCAACGCTCCTGCG‑CTGGCAG‑ACGCAACatta < 2:633078/241‑1 (MQ=255) gCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACattaa < 2:311761/249‑1 (MQ=255) cGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACattaatt < 2:916693/250‑1 (MQ=255) tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTc < 1:536598/249‑1 (MQ=255) cGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTTAATCTCTGGGAGATG‑CCGCGGAAATTCTTTTCTTTTGCGCGGATAAAATTTTCATTAACTCTCCTTCG‑CCGGCAG‑ACCCAACATTAATTACTCGc > 1:667620/1‑250 (MQ=255) cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTTGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCg > 1:302002/1‑250 (MQ=255) aaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCCCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAATCTCTGGGAAATG‑CCGCGGAAATTTTTTTCTTTGGGGCGGGTAAAATTTTCATTAACTCTCCCTCG‑CTGGGCG‑ACCCCACAATAAATTCCCCCCTTGCCGAACGcct > 2:800244/1‑248 (MQ=255) aagaagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAgtg < 2:59597/250‑1 (MQ=255) aagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGcc < 1:376158/227‑1 (MQ=255) gcgaTGACGACCGGGTGTGCTACGATATCAACGCTTCCAGCGATGGTCGTGTGGTAGATAATAGCTGGGTATCTCGCGTGGCCGAAGTGATCGACATTCCGTTGTGTGTGGCGGGTGGGAGTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGAATAAAGTGTCCATCAACTCTCCTGCT‑CTGGCAG‑ACCGAGCATGAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATtgt < 2:950034/250‑1 (MQ=255) cgcTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATtgtg < 2:937859/250‑1 (MQ=255) cTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTCTCTGGGAGATG‑CCGCCGAAATTCTTTCCTTTTGCGGGGGTAAAATTTTCATCAACCCCCCTGCG‑CTGGCAG‑ACCCAACATTAATTTCTCCCCCGGCCCACCCCTTTTGCGTGGCGGGTAtttgggg > 2:780937/1‑245 (MQ=255) tGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTTGAAGATG‑CCGCGGAAATTCTTTCCTTTTGGGCGGGTAAAAATTTCCTCAACTCTCCCTCG‑CTGGCAG‑AACCCACATTTAATAATCGCCTGGCCGCTCGCCTTGGCGGGCCGGGGATTTTGGTc > 2:167748/1‑250 (MQ=255) gACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTTCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCCCCCGGCCGATCCCTTTGGCGTGCAGTGTATTTTGGTCg > 1:829884/1‑250 (MQ=255) cGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATG‑CCGCGAAAATTTTTTCCTTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGGGTATTTTGGTCGGt > 1:125938/1‑250 (MQ=255) gAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTTGCGCGGATAAAAATTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGTGTATTTTGGTCggga > 1:246519/1‑248 (MQ=255) aCTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTAtt < 1:234539/250‑1 (MQ=255) tgaagtTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCCGAACTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCACGGAGATG‑CCGCGCAAAATTTTTCCTTTTACGCGGGGAAAATTTCCCTCCACTCCCCTGCG‑CTGGGAG‑ACCCAACAATAAATACAACACTCGCCGCGCCCTCTGGGGTGCAGCGCATTGTGGTCTGTATTgt > 2:398121/5‑249 (MQ=255) aCGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGAAGAAG‑CCCCGAAAATTTTTTTCTTTGGCGCGGGTAAAATTTTCATCAACTCTCCCTCG‑CTGGCAG‑ACCCAACATTTATTACTCTCCTGGCCGGTCGCCTTGGCGTGCAGTGTATTTTGGGCGGTATTTGTACCTGGTa > 2:309656/1‑250 (MQ=255) cGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTa < 2:374426/249‑1 (MQ=255) tatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTCTCTGGGAGATG‑CCCCGCAAATTTTTTCCTTTTGCGCGGGTAAAATTTTCATCAAATCTCCTGCGCCTGGC‑G‑ACCCCAAATTAATTACTCGCCTCGCCCGTCGCCTTTGCGTGCCGGGTATTGTGGTCTTTGTTTTTCACTGGTacgac > 2:577618/1‑250 (MQ=255) cACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATG‑CCGCGGAAATTTTTTTCTTTTGCGCGGGGAAAATTTTCATCAACTCTCCTGCG‑CTGGCCG‑ACCCCACATTAATTAATCGCCCGGCCGGTCGCTTTTGGGGGCAGTGGATTGTGGGCGGTATTGATAACTGGGAACACGcc > 2:859944/1‑250 (MQ=255) ccGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑a < 2:931729/250‑1 (MQ=255) ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAAcc < 1:747713/248‑1 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAat‑at < 2:248518/250‑1 (MQ=255) tgGTAGATAAAAGCTGGGGATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGaa < 2:167511/250‑1 (MQ=255) gATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCaa < 1:900547/249‑1 (MQ=255) aTAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAata < 2:261440/250‑1 (MQ=255) tAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAATCTCTGGGAGAAG‑GCGGGAAAATTTTTTTCTTTTGGGGGGGGAAAATTTTCATCAAACGTCCTGCG‑CTGGGGGCACCCACCATAAATT‑CTCGCCTGGGCGAGCGCTTTTGGGTGCAGGGTATTTTTGTTGGGAATGGTAACTGGTACGACGCCG‑GAAACGGGGAAT‑ATCAAGTGAATTaaaa > 2:973823/1‑247 (MQ=255) ctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGGCTCTGGGAGATG‑GCGCGGAAAATTTTTTCTTTGGGGCGGAGAAAAATTTCATCAAATCTCCTGCG‑CTGGCAG‑AACCAACATTAAATAATCGCCTGGCCGCTAGCCTTGGCGTGCCGGGGATTTTGGGGGGGATTTATACCTGGTACGGCGCCG‑GAACGGGGGAATAAACATG‑GAATCAATATACAGGCgcagga > 2:733177/1‑243 (MQ=255) tGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAAGCTCTGGGAGATG‑CCGCGGAAAATCTTTTCTTTGGGGCGGGTAAAAATTTCATCAACCCTCCTGCG‑CTGGCAG‑AACCAAAATTAATTACTCGCCTGGCCGACCCGTTTGGGGTGGAGGGGAATGTGGGCGGGATTGAAACCTGGTAAGGCGCCG‑AAAACGGGAAAA‑ATCATGTGAAACAATATACCGGCGGAGGAAGCCGCAc > 2:914084/1‑250 (MQ=255) gCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCAccc < 1:615919/250‑1 (MQ=255) cGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATG‑CCGCGAAAATTTTTTTCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCG‑CTGGCAG‑ACCCCACATTAATTACTCGCCTTGCCGATCGCTTTGGCGTGCAGTGTATTGTGGGCGGTATTTATACCTGGTACGACGCCG‑AAACCGGGAAAT‑ATCATGTGAATCAATATAACGGCGATGAAAGCCGCACCcg > 1:694923/1‑250 (MQ=255) aGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTc < 2:465821/250‑1 (MQ=255) tGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATG‑CCGCGGAAATTTTTTCCTTTGGGGCGGGTAAAATTTTCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTTGCGTGCAGGGTATTTTGGTCGGGATTTATACCTGGTACGGCCCCG‑AAACCGGTAAAA‑ATCATTTGAATCAATATAACGGCGGTGAAAGCCCCCCCCCCGTccc > 2:499078/1‑248 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAAGCTCTGGGAAATG‑CCGCGAAAATTCTTTTCTTTGGCGCGGGTAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTTATCGCCTGGCCGCTAGCTTTTGCGTGCAGTGTATTTTGGTCGGTATTTATACCTGGTACGACGCCGCAAACCGG‑AAAT‑ATCATGTGAATAAATATACCGGCGGTGAAAGACGCACCCCCGCCACt > 2:945799/1‑249 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATG‑CCGCGAAAATTCTTTTCTTTGGCGCGGGTAAAATTTTCATCAACTCTCCTGCG‑CTGGCAG‑ACCCCACATTAATTAATCGCCTGGCCGATCTCTTTGGCGTGCAGAGTATTTTGGGCGGTATTGATACCTGGTACGACGCCG‑AAAACGGAGAAA‑ATCATGTGAATAAATATACAGGGGCTGAAAGCCCCCCCCGCGACCCTc > 2:84183/1‑250 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCaa < 1:769389/249‑1 (MQ=255) tgtgtgGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTTCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTc > 1:181649/1‑249 (MQ=255) tGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAg > 1:121730/1‑250 (MQ=255) gggATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAg > 1:482234/1‑249 (MQ=255) | TGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATG‑CCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCG‑CTGGCAG‑ACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAAT‑ATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAG > NZ_CP009273/2089326‑2089816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |