Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,146	0	G	T	41.9%	88.4 / 23.3	63	intergenic (+19/+46)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base G (15/21);  new base T (4/22);  total (19/44)
Fisher's exact test for biased strand distribution p-value = 4.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGA  >  NZ_CP009273/2144901‑2145391                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             cGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTa                                                                                                                                                                                                                                                          <  2:140227/250‑1 (MQ=255) cGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTa                                                                                                                                                                                                                                                          <  1:779468/250‑1 (MQ=255)         aCTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGc                                                                                                                                                                                                                                                  <  1:420848/249‑1 (MQ=255)          cTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTATTCTTTTTTTGTTACCCATTCACAAAAAAAAAACGACGAGCGAAGAGGAAAAAAAATTTGCTAAT‑TAGCCG‑GTATAttttttttttttaaatgggg                                                                                                                                                                                                                                                 >  1:149973/1‑240 (MQ=255)             aTTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑Gtt                                                                                                                                                                                                                                             <  1:640243/249‑1 (MQ=255)             aTTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑Gt                                                                                                                                                                                                                                              <  1:937291/248‑1 (MQ=255)              ttCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTg                                                                                                                                                                                                                                            <  2:7102/249‑1 (MQ=255)               tCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGGTATAACAATTGTTCTTTTTTTGTTTCCTTCTCAAAAAAAAAAAACGAAGAGGCAAGAGGAAAAATAATTTGCTTAA‑AAACCG‑GTATTTTTTTTTTTTtaaatagggggtgga                                                                                                                                                                                                                                            >  1:795933/1‑238 (MQ=255)                      gCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTTTTCTTTTTTTTTTTCCTATCCCAAAAAAAAAACCGAACAGCCAAAAGCAAAAATAAATTTCTTAT‑CTACCG‑TTATATTTTTTTTTTTAAA‑TTGCG‑GGTggtgtttttt                                                                                                                                                                                                                                   >  2:93184/1‑241 (MQ=255)                      gCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑Ttg‑ttgt                                                                                                                                                                                                                                 <  1:791385/250‑1 (MQ=255)                         tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTATCCTTTTTTTGTTACCTATCCCAAAAAAAAAACCGACCAGCCAAGAGCAAAAATAATTTGCTAAT‑CAACCG‑TTATATTTTTTTTTTTAAA‑TTGCG‑GGTGGA‑ttt‑ttttttt                                                                                                                                                                                                                              >  1:339857/1‑245 (MQ=255)                           tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑a                                                                                                                                                                                                                           <  2:625855/250‑1 (MQ=255)                                  gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGcc                                                                                                                                                                                                                    <  1:457744/249‑1 (MQ=255)                                        tCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATAACATTTATCCTTTTTTTGTTACCTATTCCAAAAAAAAAAACGACGAGCTAAAAAGAAAAATAATTTGCTAAT‑AAACCGTTTTTTTTTTTTTTTTAAAT‑TGCGG‑GTGGAA‑TTT‑TTTTTTTT‑AA‑Cccccctaga                                                                                                                                                                                                               >  1:234306/1‑244 (MQ=255)                                         cAGAAATGCCCCTGGTATTGTAAAAAACGTAGAATAGGGCAAGAAGTGAAAAAAAAAAAAAAAAGATTTTTCAGACTGGATGTATACGCGGGGGAATAAAATTT‑ACGGCGTTTATACCATTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑GGGGG‑TTTGAA‑TTT‑TTGTCTTC‑AA‑CCGCCATGAGaaa                                                                                                                                                                                                            <  2:346378/250‑1 (MQ=255)                                                       ttATTGTTAAAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGATGGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTAATCTTTTATTGGCCCCCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTCCGCTAAC‑CAGCCT‑TTATATTTATTTTTTAAAC‑GGGCG‑GTTGAT‑TTG‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGccc                                                                                                                                                                                                <  1:885877/247‑1 (MQ=255)                                                               aaaaaggaaaaaTAGGGCAAGGAGGGAAAAATAAAGAAAAAAGATATTTCAGACGGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGTAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGCG‑TTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                      <  1:274615/240‑1 (MQ=255)                                                               aCAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAAAAAATATTTCAGACTGGATGTATCCGCGGGGGAAAAAAATTT‑ACGGGGTTTATCCCTTTTATCTTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTTAAAC‑TGGCG‑GTTAAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                      <  1:788060/250‑1 (MQ=255)                                                                cAAACGTAGAATGGGGCCGGGAGTGAAAAACAAAAAAAGAAAATATTTCAGACTGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAACCCGACGAGAAAAGAGAAAAATTGGTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt                                                                                                                                                                                     <  1:865507/250‑1 (MQ=255)                                                                       aGAATAGGGCAAGGAGTGAAAAAAAAAAAAAAAAGATATTTCAGATGGGATGTATCCGGGGGGGAAAAAAATTT‑CGGGGGTTTATCCCATTTATCTTTTATTGGTCCCCTAACAAAAAGAAGAAGCCGAGGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CGGCCT‑TTATTTTTATTGTTAAAAC‑GGGCG‑GTTAAT‑TTT‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg                                                                                                                                                                              <  2:306955/250‑1 (MQ=255)                                                                             gggCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGACTGGATGTATCCGCGCGGGGATAAAATTT‑CCGGCGGTTATACCTTTGATCCTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTa                                                                                                                                                                         <  1:305662/248‑1 (MQ=255)                                                                              ggCCGGGAGTGAAAAATAAAGAAAAAAGATATTTCAGACTGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                       <  1:98006/250‑1 (MQ=255)                                                                                          aaaaaaaaaaaagaaGATATTTCAGACTGGATTTATCCGCGGGGGGAAAAAATTT‑CCGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                                                                                                           <  1:172017/241‑1 (MQ=255)                                                                                              caaaaaaaaaaGTTTTTTCAGCCGGGTTGTATCCGCGGGGGAAAAAAATTG‑AGGGCGGTAACACTTTTGATCTTTTATTGTCCCCCTACCAAAAAGAAGAAGCCGACGAGAAAAGAAAAAAATTAGTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGGG‑TTTGAT‑TTT‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCg                                                                                                                                                       <  2:522690/245‑1 (MQ=255)                                                                                                             ttacAGACTGGTTGTTACCGCGGGGGAATAAAATTT‑AGGGGGTTTAACCTTTTGTTCTTTTATGGGCCACCTAACAAAAAGAAGAAGCCAACGAGAAAAAAAAAAATTTATTTCGCTAAC‑CACCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTAC‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCt                                                                                                                                         <  2:185763/246‑1 (MQ=255)                                                                                                                  gACGGGTTGTATACGCGGGGGAAAAAAATTT‑CCGGCGGTTATCCCTTTGTTCTTTTTTTGGTCCCCTACCAAAAAAAAAAAGCCGAGGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑TGGGG‑TTTAAA‑TTT‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaa                                                                                                                                   <  1:401135/250‑1 (MQ=255)                                                                                                                   acgggATGTATACGCGGGGGAATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAC‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGGG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaa                                                                                                                                  <  1:666117/247‑1 (MQ=255)                                                                                                                       gATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATGGGTCACCTACCAAAAAAAAGAACCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                                                                                              <  1:552510/250‑1 (MQ=255)                                                                                                                                cggggtgaaaaaatTTT‑ACGGCTGTATTCCTATTTTTCTTTTTTTGGCCCCCTATAAAAAAGAAGAGGCCGACGAGTAAAAAGAAAATTTTGTTC‑ATAATGCGGCTT‑TTTTTTTTATTTTTTAACC‑GGGCG‑GTTTAT‑TTG‑TTATCTAC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                     <  2:889170/240‑1 (MQ=255)                                                                                                                                 gcgggggAAAAAATTTGACGGTGATTATACCATTTATCTTTTATTGATCACCTACCAAAAAGAAGAAGCCGAGAAAAAAAAAAAAAAATTATTCCCCTAAC‑CAGCTT‑TTATTTTTTTTG‑TTAAAA‑GGGGG‑GTTGAA‑TTT‑TTTTTTTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                     <  2:154656/247‑1 (MQ=255)                                                                                                                                   gggggATAAAATTT‑ACGGGGGTTATCCCTTTGATCTTTTTTTGGTCCCCTATCAAAAAAAAAAAGCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTAAAAC‑GGGGG‑TTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAtt                                                                                                                  <  1:703846/249‑1 (MQ=255)                                                                                                                                            aaTTT‑ACGGAGGTTATACATTTGATCCTTTATGGGTCACCAATCAAAAAGAAAAGGCCAAAGAGCAAAGAGAAAATTTAGTTCGATAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑GGGCG‑GTTGAA‑TGG‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata                                                                                                         <  1:798132/250‑1 (MQ=255)                                                                                                                                             tttt‑AGGGCGTTTATACCATTAATCCTTTATTGGTCACCTATCAAAAAAAAAAGCCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAACC‑TGGGG‑TTTGAA‑TTG‑TTTTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaataa                                                                                                        <  1:737415/249‑1 (MQ=255)                                                                                                                                                  ccggcgtTTATCCTTTTGATCTTTTTTTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTGAT‑TTT‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                                                                                                     <  1:607010/248‑1 (MQ=255)                                                                                                                                                          tatcCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAGCCCGAGGAGAAAAGAGAAAAATTAGTCCTCTAAC‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                             <  1:493130/249‑1 (MQ=255)                                                                                                                                                               cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTTTCTTT‑AA‑CCCCCATGAGAAAATCACCCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCGCTTACCCCCCGGCTGGGCAAAAAATGCTTTAACAATTCCGGTAATAAATTGAGTTGTTTCTGCg                                                                                       >  2:453155/1‑250 (MQ=255)                                                                                                                                                                  tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTTTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAAAGTTTTATTATCATCCGTACGCTCGTTTAACGCCGGGCCGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTGTTTCTTCGttt                                                                                    >  2:937216/1‑249 (MQ=255)                                                                                                                                                                  tGACCCTTTATGGGCCCCCTACCAAAAAAAAGAGCCCGACAAGCAAAAAGAAAATTTATTTCGCTAAT‑CGGCCT‑TTATATTTATTTTTTAAAC‑TGGGG‑TTTAAT‑TTG‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt                                                                                     <  1:229380/249‑1 (MQ=255)                                                                                                                                                                   gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGGA‑TTT‑TTGTCTTC‑AA‑CCGCCCAGTGAAAATTTCTCTGCCCACCGCGCCAGTGCCAAAGTTTTATTATCATCCGTACGCCCGTTTACCGCCCGGCCGGACCAAAAACGCTTTAACAATTCAGGTAATAAATCGAGTTTTTCCTGCCTt‑gc                                                                                  >  2:535991/1‑250 (MQ=255)                                                                                                                                                                    aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTTT‑TA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATAATCCGGACGCTCGTTTACCCCCGGGCCGGACAAAAAATGCTTTAAAAATTCAGGGAATAAATCGAGGTGTTCCTGCGGt‑gct                                                                                 >  2:212040/1‑250 (MQ=255)                                                                                                                                                                       cTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTCTTC‑AA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                              >  2:187068/1‑250 (MQ=255)                                                                                                                                                                        tttATTGTTCACATACAAAAAAAAAGAGACCGAGGGGATAAGAGAAAAATTATTTCGTTACC‑CTCCTT‑TTATATTT‑TTTTTTAAAC‑GGGGGTGTTAAT‑TTG‑TTTTCTTC‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                              <  2:862873/249‑1 (MQ=255)                                                                                                                                                                         ttATTGGCCCCCTACCAAAAAAAAGAAGCCAACGAGCTAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGCG‑GTTAAA‑TTG‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgccg                                                                             <  1:588244/249‑1 (MQ=255)                                                                                                                                                                              ggacacttaccaaaaaaaAGAGGCCGACGAGAAAAGAGAAAAATTGGTCCGATAAT‑CGGCGT‑TTATTTTTTTTTTTAAACC‑GGGCG‑GTTAAT‑TTT‑TTGTCTTA‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGt                                                                        <  1:967687/237‑1 (MQ=255)                                                                                                                                                                               tccACCTAACAAAAAAAAAAAGCCGACGACATAAGAGAAATATTAGTTCGTTAAT‑CATCTT‑TTTTTTTTATTTTTTAAGC‑GGGGG‑GTTGAA‑TT‑‑TTTTCTTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                      <  2:788841/248‑1 (MQ=255)                                                                                                                                                                               gtccccaaccaaaaaaaaaaaGCCGAAGAGCAAAGAAAAAAATTATTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                      <  1:715469/239‑1 (MQ=255)                                                                                                                                                                                 cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCCCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCCACGGTTTTTCCTCATCCGTACGCCCGTTTACCGCCCGGCTGGACAAAAAATGCTTTAAACATTCAGGGACTAAATACAGGTGTTTCTGCGTT‑GGTGCCCCCAGTCCAt                                                                    >  2:306988/1‑250 (MQ=255)                                                                                                                                                                                          aaaaGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGTA‑TTG‑TTGTCTTT‑AA‑ACGCCATGAGAAACACACTCTGCCCACAGCGCCACTGCCCACGGTTTTTTATAATCCCCACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTTACAATTCCGGTAATAAATCGAGGTGTTCCTGCGTT‑TCTGCCGCCCATCCATTTaaaaaag                                                           >  2:722512/1‑248 (MQ=255)                                                                                                                                                                                               aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAAAGGGGt                                                       >  1:145425/1‑249 (MQ=255)                                                                                                                                                                                                     ccgcgaaGCAAAGAGAAAAATTATTCCGCAAAC‑CAGCTT‑TTAT‑TTTTTTTTTTAAAC‑CGGGG‑GTTTAATTTT‑TTTTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                <  1:484573/244‑1 (MQ=255)                                                                                                                                                                                                     ccgccggcatAAGAAAAAAATTGGTTGGTTAAC‑CAGCCT‑TTTTTTTTATT‑TTTAAACTTGGCG‑TTTGAG‑TTG‑TTTTCTCC‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                <  1:530625/241‑1 (MQ=255)                                                                                                                                                                                                        acgaGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAgg                                              >  1:91553/1‑249 (MQ=255)                                                                                                                                                                                                            gcaaaGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑TGGCG‑TTTAAT‑TTT‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                         <  1:890235/247‑1 (MQ=255)                                                                                                                                                                                                                agagAAAAATTAGTTCGTTAAC‑CGGCTG‑TTATTTTTTTTGTTTAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTAA‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                     <  1:455869/250‑1 (MQ=255)                                                                                                                                                                                                                       aaTTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTGCGGT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTa                              >  1:815528/1‑250 (MQ=255)                                                                                                                                                                                                                         ttAGCTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTGCTGGTCTCC‑AA‑CCCCCA‑GAGTAAATCACTCCGGCCACAACGGCAGGGCCAACGCTTTTTTAACATCCCTGTGGTCCCTTACCGCCGGGGCGGGGAAAAAAAGGTTTTACAACTTAGGGAATAAATAGTGGTGGTCCTTCGTTGGCTG‑CGCCAGGTCCTTGAAAAAAGGGGGAAATAAAGGCATATGAGGGGAAATATc                            >  2:590504/1‑250 (MQ=255)                                                                                                                                                                                                                           attccGCTAAC‑CAGCTG‑TTATATTTATTTTTAAACC‑TGGCG‑TTAGAT‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                           <  1:675982/245‑1 (MQ=255)                                                                                                                                                                                                                                  tAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                   >  1:950077/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaaccacctg‑TTATTTTTTTTTTAAAAAC‑TGGGG‑GTTAAA‑TTT‑TTTTTTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCata                     <  1:127224/239‑1 (MQ=255)                                                                                                                                                                                                                                    aaaccatcttttATATTTTTTGTTAAAAC‑GGGGG‑TTTGAT‑TTG‑TTGTCTAA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                   <  1:92991/240‑1 (MQ=255)                                                                                                                                                                                                                                               tataTTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTTT‑AA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTTGACAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGTTGTTTCTGCGTT‑GCTTCCGCCAGTCCCTTGAAAAAAGGGGTAAAGAAAGGCACATGAGGGGAATTATCCAGCATATTTAAAGCCAggc        >  2:611500/1‑248 (MQ=255)                                                                                                                                                                                                                                                     tattTTTAAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTgg   <  1:93184/249‑1 (MQ=255)                                                                                                                                                                                                                                                      attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTTTTT‑AA‑CCGCCAAGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCCCCCGGCTGGACAAAAAATGCTTTAACAAATCAGGGAATAAAACGAGTTGTTTCTGCGTT‑GCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGAATATTTAACGCCAGTCGCTggg  >  2:352666/1‑248 (MQ=255)                                                                                                                                                                                                                                                      attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa  >  1:918683/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             CGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGA  >  NZ_CP009273/2144901‑2145391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑