Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,036,390	0	A	G	47.2%	53.2 / 17.3	53	N206D (AAT→GAT)	srkA	stress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base A (18/10);  new base G (25/0);  total (43/10)
Fisher's exact test for biased strand distribution p-value = 8.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCG  >  NZ_CP009273/4036145‑4036639                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGGTTTTACCGTTTTGCGGCTACATGGGGAATGCCCCGCCGGGAatat                                                                                                                                                                                                                                                        >  1:616751/1‑250 (MQ=255)    ttGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGTGAGATTTCGTCCTTCTGCGTCGACATCGAGACCACCGCGCCGCGGATATtct                                                                                                                                                                                                                                                     >  2:46959/1‑250 (MQ=255)        gCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTgg                                                                                                                                                                                                                                                 >  1:103404/1‑250 (MQ=255)                              cAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATTGGGACTGCCACGCCGGGAATATTCTCTTGCGCGGT‑GGTCCAATGTttgttg                                                                                                                                                                                                                          >  1:597306/1‑250 (MQ=255)                                gACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGGTACATGGAGAATGCCACGCCGGGGATATTTTCTTGCGCGGT‑GGGTCAATGTttgttgtt                                                                                                                                                                                                                        >  2:162405/1‑248 (MQ=255)                                 aCGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCACATGGAGACTGCCCCGCCGCGGAGATTTTCTGGCGCGCT‑GGTCCCAtgtttgtttttc                                                                                                                                                                                                                       >  2:699873/1‑246 (MQ=255)                                        gcAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATAtt                                                                                                                                                                                                                                                       <  1:106947/212‑1 (MQ=255)                                              aGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCCGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACg                                                                                                                                                                                                          >  1:96277/1‑250 (MQ=255)                                                  ttttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGGGGCTACATGGAGAATGCCCCGCCCGGGGTATTCTCTGGCGCGCT‑GGTGCAATGTTTGTTGTTCTGGATGATGCACGTAAt                                                                                                                                                                                                      >  2:292005/1‑250 (MQ=255)                                                       tCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGGAACTGGCCCGCCGGGGATATTCTCTGGCGCGGT‑GGGCCAATTTTTTTTGGTCTGGGTGGTGCCCGGAATGGTc                                                                                                                                                                                                  >  2:463850/1‑249 (MQ=255)                                                       tCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGAT‑GGGCCAATGTTTTTTTATCTTGATGATGCACGTAATGGTc                                                                                                                                                                                                  >  1:336272/1‑249 (MQ=255)                                                        ccATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCAATGTTTTTTGGTCTTGATGATGCACGTAATGGTc                                                                                                                                                                                                  >  1:37727/1‑248 (MQ=255)                                                         cATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCCTTCTGCGGCCACAAGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGGT‑GGGCCAATGTTGTTTGTTCTTGAAGGTGCACGTAATGGTTCAg                                                                                                                                                                                               >  2:431827/1‑250 (MQ=255)                                                           tCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGcc                                                                                                                                                                                             >  1:25350/1‑250 (MQ=255)                                                             gCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGAGACTGGCCCGCCCGGGATATTCTCTGGCGCGGT‑TGTCCAATGTTTGTTTGTCTTGGTGATGGACGTAATgggtccgccgg                                                                                                                                                                                           >  2:459095/1‑241 (MQ=255)                                                               ccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGCTc                                                                                                                                                                                         <  1:61222/250‑1 (MQ=255)                                                                 gACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTTTGCGGCTACATGGAGACTGCCACCCCGGGGATATTCTCTGGCGCGGT‑GGGCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGGTCAg                                                                                                                                                                                       >  1:873538/1‑250 (MQ=255)                                                                   ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTCTTCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGGCCAGCCGTTCCGGa                                                                                                                                                                                     >  1:355413/1‑250 (MQ=255)                                                                        ggTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGCT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTtgt                                                                                                                                                                                >  1:340526/1‑250 (MQ=255)                                                                                    tatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTTGCGCGGT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTc                                                                                                                                                                     >  1:190104/1‑249 (MQ=255)                                                                                         cATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGCT‑GGTCCAATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGTTCAATggg                                                                                                                                                               >  1:454559/1‑249 (MQ=255)                                                                                                ccACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAAATTTTACCGTTCTGCGGCCACATGGGGACTGCCCCCCCGGGGATATTTTCTTGCGCGGT‑GGTTCCATGTTTGTTTGTTTGGGTGATGGACGTAATGGTTCAGCCGTTCCGGGTTTTTGGGGGGTGGTCAATTGGGGTaaa                                                                                                                                                         >  2:300165/1‑249 (MQ=255)                                                                                                         cTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTCCCGTTCTGCGGGTCCATGGAGACTGCCACGCCCGGGATATTTTCTTGCGCGGT‑GGGCCAATGTTTGTTGATCTGGGTGATGCACGTAAAGGTTCAGCCGGTCTGGGTTTGTGGTTGTTGCGTAATGGCGATAAAAGCGagcaac                                                                                                                                               >  2:915491/1‑248 (MQ=255)                                                                                                            tttGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGCGGCCACACGGGGACTGCCCCCCCGGGGATATTCTCTGGCGCGGT‑TGTCCAATGGTTTTTGGTCTGGATGATGCACGTAATGGTCCACCCGTTCCGGGTTTTTGGATGGTGCTCCATGGCGGTAAAGCCCAGGCGCggg                                                                                                                                            >  2:254162/1‑249 (MQ=255)                                                                                                            tttGAGGACGCTACACTGATACCTCCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCgg                                                                                                                                             <  2:394042/249‑1 (MQ=255)                                                                                                                                 cttcccGGGTGGAAAGCGGCATTCCTGAAGGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTg                                                                                                                       <  2:933732/246‑1 (MQ=255)                                                                                                                                  cTTCCGGGTTGAAAGTGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTTGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTTCTCAATTGCGATAAAACCGAGCAGAGGATGCAACTGGGAACTatttttt                                                                                                                       >  1:222851/1‑248 (MQ=255)                                                                                                                                     ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGGT‑GGTCCCAATTTTTTTGATCTGGGGGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTTCTCCATGGCGATGAAAGCGGGACGAGGGTGGGACTGGGAAATATTTTTGaaac                                                                                                                   >  2:136211/1‑248 (MQ=255)                                                                                                                                     ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGAT‑GGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGGTCAGGATTTGTGGGTGGTGCTCAATGGCGATAAAACCGAGCAGCGGGTGCAACTGGGAACTATTATTGAAGc                                                                                                                   >  1:552530/1‑250 (MQ=255)                                                                                                                                         gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCGACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCCGGATTTTTGGAGGTTGCTCAATGGCGGTAAAAACGAGCAGCGGGTGCAACTGGAAAATATTATTTAAACTTAt                                                                                                               >  2:412158/1‑250 (MQ=255)                                                                                                                                          ttGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGGCACATGGAGACTGCCACGCCGGGGATATTTTTTGGGGCGGTGGGTCCAA‑GTTTGTTTATCTGGGTGGTGCACGGAAAGGGCCAGCCGGTCCGGGTTTGTGGGTGTTGGTCAATGGCGGTAAAACCGGGGAGCGGGAGGCACTGGAAAATTTTTTTTAAGCTTTTg                                                                                                              >  2:443951/1‑250 (MQ=255)                                                                                                                                            gAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                                                             <  2:167912/249‑1 (MQ=255)                                                                                                                                                     aTTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGc                                                                                                   <  1:909349/250‑1 (MQ=255)                                                                                                                                                      ttCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGGTCAGGATTTTTGGATGTTGCTCAATGGCGGTAAAACCGAGCAGCGGGTGCAACTTGAAAATATTATTTAAGCTTATGAAGAATTTAGCg                                                                                                  >  1:231858/1‑250 (MQ=255)                                                                                                                                                               gCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCTGCTACAAGGTGACCGCCACCACCGGGATATTCTTTTGCGCCAT‑GGTCCCATGTTTTTTGAACTGGGTTATTCACGTAAAGGTGCAGCCGTTTAGGGTTTGTGGATGTTGATCAATGGCGGTAAAGCCGAGCAGCCGGTGGAAATGGGAAAGATTATTGGAGGCTATGAAGAAGTTAGAGGGTTCggc                                                                                          >  2:390948/1‑247 (MQ=255)                                                                                                                                                                 gACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCTCCATGGAGACTGCCCCGGCGGGGATATTTTCTGGCGCGGT‑GGTCCCATGTTTTTTTATCTGGGTGATGCACGTAATGGGCCAGCCGGTCCGGGTTTTTGGGTGTTGGTCCATGGCGATAAAGCCCGGCAGCGGGTGCAAATGGAAACTATTATTTAAGCTTATGAAAAATTTATAGGGTTCGaccac                                                                                       >  2:892750/1‑247 (MQ=255)                                                                                                                                                                   cAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGc                                                                                     >  1:476103/1‑250 (MQ=255)                                                                                                                                                                      aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGGT‑GGGCCCATGTTTTTTGATCTGGATGATGCACGGAATGGGCCAGCCGTTCCGGGTTTGTGGGTGTTGCTCCATGGCGATAAAACCGAGCAGCGGGGGGAACTGGGAAATATTATTTAAGCTTTTGAAGAAATTAGCGGGTTCGACACCGccg                                                                                   >  2:528790/1‑247 (MQ=255)                                                                                                                                                                                 gccgccGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGGGAATGCCCCGCCGGGGATATTTTCTGGCGCGAT‑GGGTCCATGTTTGTTGATCTGGATGATGCACGCAATGGGCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGGGATAAAGCCGAGCAGCGGAGGGCACTGGGAACTATTTTTGAAGCTTTTGAAGAAATTTGCGGGTTCGACACCGCGGGAAACGGGCTgg                                                                       >  2:661553/1‑249 (MQ=255)                                                                                                                                                                                      cgTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCGACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGGT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGTTCAATGGCGATAAAGCCGCGCAGCGGATGGAACTGGGAACTATTATTTAAGCTTATTAAGAATTTAGCGAGTTCGACAACCCTGAAATCGGACTGATTGaa                                                                  >  2:360949/1‑250 (MQ=255)                                                                                                                                                                                            aGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTa                                                            <  1:214751/250‑1 (MQ=255)                                                                                                                                                                                                            gATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg                                            >  1:89167/1‑250 (MQ=255)                                                                                                                                                                                                               ttCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGtt                                          >  1:857183/1‑249 (MQ=255)                                                                                                                                                                                                                tCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGttt                                         <  1:76511/249‑1 (MQ=255)                                                                                                                                                                                                                 cACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttat                                       >  2:18911/1‑250 (MQ=255)                                                                                                                                                                                                                   ccGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttatta                                     >  2:382770/1‑250 (MQ=255)                                                                                                                                                                                                                         tGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGc                               >  2:529304/1‑250 (MQ=255)                                                                                                                                                                                                                           cGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCt                             >  2:29796/1‑250 (MQ=255)                                                                                                                                                                                                                              cTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGc                          >  2:880951/1‑250 (MQ=255)                                                                                                                                                                                                                              cTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGc                          >  2:927347/1‑250 (MQ=255)                                                                                                                                                                                                                                 cATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTaa                       >  2:66734/1‑250 (MQ=255)                                                                                                                                                                                                                                                gCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTg        <  1:699873/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ccGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGa       <  2:184097/250‑1 (MQ=255)                                                                                                                                                                                                                                                      aaTATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCcg  >  1:147001/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           CGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCG  >  NZ_CP009273/4036145‑4036639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑