Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,753,7440AC39.6% 51.1 / 16.8 48V289G (GTG→GGG) selAL‑seryl‑tRNA(Sec) selenium transferase
Reads supporting (aligned to +/- strand):  ref base A (21/8);  new base C (1/18);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 3.79e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAA  >  NZ_CP009273/3753501‑3753980
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
cGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCCTCTCTTTTTTTCCCACAATAATTCCTGCCTGCGGCCCGCCCAACCACTTTTCGCCGGAGAAACTCCCCAGAc                                                                                                                                                                                                                                         >  2:442732/1‑250 (MQ=255)
cGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGAc                                                                                                                                                                                                                                         >  1:256024/1‑250 (MQ=255)
                     cccTCGGCGGTGGGGGAAAGCGGGCGCGGGGTCGGTAATTTTCCACTCAGAGCTTCAGGGTGAAAAAAAAGACGCACCGTGGCTCCCAGCGCCGCGAGGGTCTTTTTACCCGCGCGTAATGCCCGCTTCAGCGGGGGGTTTGGCAGGCGGGCGATCATCTCTTTTTTCCCAACAAAAATTCCTGCCGGCGCCCCGCCTAACAACTTGTCGCCGGAGAAACCCACCAGACTGACGCCCGCCGCAATCAACt                                                                                                                                                                                                                    <  2:468198/249‑1 (MQ=255)
                           gcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCCAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCg                                                                                                                                                                                                              >  1:315164/1‑250 (MQ=255)
                                 cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCAttt                                                                                                                                                                                                        >  1:41489/1‑249 (MQ=255)
                                      aaGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCCACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTTTTGCCGGGGAAACTCACCAGACTGACGCCCGCCCCAAACAACTCCCGCCGCCTTTGctct                                                                                                                                                                                                   >  2:221271/1‑250 (MQ=255)
                                         cAGGCGCGGGGTCGGTAATTTTTCCCACAGAGCTTCAGGGTGTAAATAAAGACGCAAGGGGGCTTCCAGCGCCGCGAGGGTCTTTTTACCCGCGCGTAATGCACGCTTCGGGGGGTGGTTTTGCGGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTGGTCGCCGGAAAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTc                                                                                                                                                                                                <  2:97651/250‑1 (MQ=255)
                                           ggCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCgg                                                                                                                                                                                              <  1:135072/250‑1 (MQ=255)
                                                   gTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGt                                                                                                                                                                                      <  1:136979/250‑1 (MQ=255)
                                                   gTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCg                                                                                                                                                                                       >  1:309854/1‑249 (MQ=255)
                                                           tttttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCt                                                                                                                                                                               >  1:435239/1‑249 (MQ=255)
                                                            ttttCACACAGAGCTTCAGGGTGTAAAAAAAGACGCAACGGGTCTCCCAGCGCCGGGGGGGTAATTTTACCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTGGCGGGGGGGCGATCATCTCTTTTTTACCAACAAAAATCCCTGCCGGCGGCCCGCCTAACAACTTGTCGCCGGAGAACCCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTa                                                                                                                                                                              <  2:311360/249‑1 (MQ=255)
                                                                  cTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTTTCGCCGGAGAAAATCACCAGACTGCAGCCCGCCGCAATCCAATCCTGCGGCATTGGCTCCTTCGGCAAAACGTCCTGGCCAAGATCGa                                                                                                                                                                       >  2:606558/1‑250 (MQ=255)
                                                                   tCAGAGCTTCAGGGCGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCACCTCTTTTTTTCCAAAAAAAATTCCCGCCTGCGGCCCGCCTAACAACCTGTCGCCCGAGACACTCACCCGACTCACGCCCCCCCCAACCAACTCCTCCCGCATTGGCCCTTTCCGCACACCCGACCGCCTAAGATCAAc                                                                                                                                                                      >  2:278366/1‑250 (MQ=255)
                                                                        gCTTGAGGTTGAAAATAAAGACGCAACGTGGCTCCCAGCGCCGCGGGGTTCTTTTTACCCGCGCGTAATGCCCGCTACGGGGGGTGGCTTTGCAGGCGGGCGATCATCTTTTTTTTACCAACAATAATTCCTGCCGGCGCCCCGCCTAACAACTGGTCGCGGGAAAAACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCg                                                                                                                                                                 <  2:94283/250‑1 (MQ=255)
                                                                         cTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCCCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGGAATGGCCAAGATCGACCAGc                                                                                                                                                                  >  1:188017/1‑248 (MQ=255)
                                                                            cAGGGTGTAAAAAAAGACGCAACGTGGCTCCCAGCGCCGCGGGGGTCTTTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCGGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAg                                                                                                                                                               <  1:357586/248‑1 (MQ=255)
                                                                                 tgtAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACCGc                                                                                                                                                        >  1:358349/1‑250 (MQ=255)
                                                                                       taaaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTTCCTGCGGCCCGCCTAACAACTTTTCGCCGGAGAAAATCACCAGAATGACGCCCGCCCCAATCAACTCCTGCCGCATTTGCTCTTTCTGCCAACCGTACTGGCTAAGATCGACCAGCGCGCCCCCGCCCAAAt                                                                                                                                                  >  2:607503/1‑250 (MQ=255)
                                                                                          aGACGCACCGTGGCTCCCGGCGCCGGGGGTTTCTTTTTCCCCGCGCGTAACCCCCGCTCCGGGGGGGGGTTTTGCAGGCGGGCGCTCCTCTTTTTTTCCCCAAAAATAATCCCTGCCTGCGCCCCCCCAAACAACTTGTCGCGGGAAAACCCCACCAGCCGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAg                                                                                                                                               <  2:144123/250‑1 (MQ=255)
                                                                                                aggggggCTCCCGGCGCGGCGGGGTTCTTTTTACCCGCGCGTAATGCACGCTGCAGCGGGTGGCTTTGCGGGGGGGCGATCATCTCTTTTTTACCAACAATAATCCCTCCCTGCGGCCCCCCTACCAACTGGGCGCGGGAAAACCCCCCCAGCCGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTa                                                                                                                                         <  2:608554/245‑1 (MQ=255)
                                                                                                             gcgcCGCGAGGGTCATTTTACCCGCGCGTAATGCACGCTTCAGCGGGTGGTTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAg                                                                                                                            <  1:565713/250‑1 (MQ=255)
                                                                                                                         tctttttACCCGCGCGAAATGCCCGCTCCAGGGGGGGGCTTGGCGGGCGGGCGTTCATCTTTTTTTTACCAACAATAATCCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAg                                                                                                                <  1:416973/247‑1 (MQ=255)
                                                                                                                            ttttATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGACACCGCCTAAATCAGTCACCACGGGGACATCCAGCTCTTTGCCGAgcgc                                                                                                             >  2:543160/1‑250 (MQ=255)
                                                                                                                                    gctgttattGCCCGTTTCAGCGGGGGGTTTTGCGGCGGGGCGACCTTCTTTTTTTTCCCAACAATATTCCCTGCCTGCGCCCCGCCAAACACCTGGGCGCCGGAAAACCCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGtt                                                                                                     <  2:287562/242‑1 (MQ=255)
                                                                                                                                            cccccGCTTCAGCGGGTGCTTTTGCGGGCGGGCTACCTTCTTTTTTTTACCAACAAAAATCCCTCCCTGCGCCCCCCCAAACAACTTGTCGCCGGAAAACCCCCCCAGCCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCa                                                                                             <  2:561358/246‑1 (MQ=255)
                                                                                                                                                      aGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCtt                                                                                  >  1:150757/1‑250 (MQ=255)
                                                                                                                                                       gggggTGGCTTGGCGGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACACCTTGTCGCGGGAGAAACTCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCttt                                                                                 <  1:413525/248‑1 (MQ=255)
                                                                                                                                                                       gcgggcgTTCATCTCTTTTTCACCAACAATAATCCCTCCCTGCGGCCCGCCTAACATCTTGCCGCCGGAAAAACCCACCAGCCGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATg                                                                 <  1:319298/250‑1 (MQ=255)
                                                                                                                                                                          tgcgATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGCTAGACCAGTCCTCCCATGCCTAAATCAGTCACTACGGCCACATCCAGCTCTTCTCCGAGCGCCACCAGTTCCGC‑TCACCATATCGATTTGGTGAAccccatccttctg                                                              >  2:200353/2‑241 (MQ=255)
                                                                                                                                                                                catcTCTTTTTTCCCAACAATAATCCCTCCCTGCGCCCCGCCAAACAACTTGCCGCCGGAGAAACCCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                         <  1:121247/249‑1 (MQ=255)
                                                                                                                                                                                catcTCTTTTTTACCAACAATAATCCCTCCCTGCGGCCCGCCTAACAACTTGTCGCGGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                         <  1:543160/249‑1 (MQ=255)
                                                                                                                                                                                 ctcttTTTTTCACAAACAATAATTCCTCCCTGCGGCCCCCCTAACAACTTGTCGCCGGAAAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTAc                                                       <  1:75478/249‑1 (MQ=255)
                                                                                                                                                                                 atcTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTAc                                                       >  2:196621/1‑250 (MQ=255)
                                                                                                                                                                                      ttttttACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTa                                                  >  1:381228/1‑250 (MQ=255)
                                                                                                                                                                                          tacccAAAAAAAATCCCTGCCGGCGGCCCGCCTAACAACTTGTCGCGGGAGAAACCCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGt                                               <  1:442732/246‑1 (MQ=255)
                                                                                                                                                                                             ccAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACtt                                           >  1:391698/1‑250 (MQ=255)
                                                                                                                                                                                               aacaaTAATCCCTGCCTGCGGCCCGCCTAACACCTTGTCGCCGGAGAACCCCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtc                                         <  1:162307/250‑1 (MQ=255)
                                                                                                                                                                                                 caaTAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcat                                       >  1:564004/1‑250 (MQ=255)
                                                                                                                                                                                                 caaTAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcat                                       >  1:526286/1‑250 (MQ=255)
                                                                                                                                                                                                        ccctgcgtgCGCCCCCCCAAACACTTTGCCGCCGAAAAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAg                                 <  1:338454/248‑1 (MQ=255)
                                                                                                                                                                                                           tgcctgcGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCg                             >  2:40932/1‑250 (MQ=255)
                                                                                                                                                                                                                     cccccaaaaaccTTGCCCCCGGAGAACCCCCCCAGCCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcat                   <  2:4475/239‑1 (MQ=255)
                                                                                                                                                                                                                       gCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcatt                  >  1:64648/1‑249 (MQ=255)
                                                                                                                                                                                                                       gCCTAACAACTGGTCGCCGGAAAAACCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc                 <  1:273260/250‑1 (MQ=255)
                                                                                                                                                                                                                                   gTCGCGGGAAAACCTCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGAc       <  1:454756/248‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCCGGAAAACCCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATa   <  1:606299/250‑1 (MQ=255)
                                                                                                                                                                                                                                      gCCGGAAAAACCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATaa  <  1:1933/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
CGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATC‑TATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAA  >  NZ_CP009273/3753501‑3753980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑