| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,161 | 0 | C | T | 40.0% | 77.2 / 24.8 | 60 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (8/28); new base T (24/0); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAG > NZ_CP009273/4593914‑4594408 | gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt < 1:248655/250‑1 (MQ=255)gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct < 2:15070/249‑1 (MQ=255)gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAGtttttctt > 2:341614/1‑250 (MQ=255) tAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt > 2:384677/1‑250 (MQ=255) aaaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt < 2:121390/250‑1 (MQ=255) gcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga < 1:332015/250‑1 (MQ=255) cAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag < 2:76271/250‑1 (MQ=255) gggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa < 2:97930/250‑1 (MQ=255) cAAGAGAACTTAACTTGCCTTCTGTTTATGGAGAAGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg < 1:515583/250‑1 (MQ=255) aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc < 1:38618/250‑1 (MQ=255) acttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg < 2:493376/249‑1 (MQ=255) cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGGAGGGAGCGCCGGGAGGGGtttttt > 1:453890/1‑246 (MQ=255) tCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTCttt > 1:244136/1‑249 (MQ=255) gTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCCGAGAGGGGTTAtttttctt > 1:235004/1‑246 (MQ=255) tGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTGTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact < 1:525991/249‑1 (MQ=255) agaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGAGCAGAGGGGGGTTATCTTGCCTGATTGaaa > 2:68644/1‑249 (MQ=255) ccGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCg < 1:597096/250‑1 (MQ=255) gCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCGGAGAGGGGTTTTCTTGTCTGAGTGTACT‑GGGGAt > 2:137073/1‑250 (MQ=255) cGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGTCTGAACT‑GGGGATTAAGTTTTCGCGTAc‑cc > 2:260543/1‑250 (MQ=255) aaTTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGGTATCTTTCCTGACTGTACT‑GGCGGTTTAGTTTTCGCGTAC‑CCgg > 2:415182/1‑250 (MQ=255) aaTTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGGGCGCAGAGAGGGATTATTTTGCCCGACTGAACT‑GGCGATTAAGGTTTCGCGTAc‑cccg > 1:247378/1‑248 (MQ=255) tCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCgg < 1:456/249‑1 (MQ=255) cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACT‑GGCGATTAAGGTTTCGCGTAA‑CCGGCGTTTTGTTATTGCGgatgat > 1:385114/1‑249 (MQ=255) tCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGGGCGGAGAGAGGGATTATTTTGCCCGACTGAACT‑TGCGATTTAGTTTTCGCGGAC‑CCGGCGGTTAGTTATTGCGgttgatga > 1:518511/1‑250 (MQ=255) aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCCGGGGGGGGTTATCTTGCCTTGCTTAACT‑TGCGGTTTAGGTTTCGCGGAC‑CCGGGGTTTATTTATTGCGggtggttggattt > 2:128835/1‑240 (MQ=255) aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGGAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACT‑GGCGGTTAAGGTTCCGGGTAC‑CCGGGGTTTAGTTATTGCGGATGATGATAttt > 2:83789/1‑248 (MQ=255) aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGGGAAGTGAGGGGCGCGAGGGGGTATCTTTCCTGACCGGACT‑GGCGGGTTAGGTTCCGCCGTC‑CCGGCGGTTTGTTTTTGCGGGTGATGGTAttta > 2:309063/1‑247 (MQ=255) aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAGCGCCGGGGGGGGTTTTTTTTCCTGGCTGAACT‑GGGGGTTAAGGTTTCGCGTGA‑CCGGCGGTTTGTTTTTGGGGGTGATGAtttttt > 2:71567/1‑247 (MQ=255) aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑Ga < 1:57565/250‑1 (MQ=255) tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GAt < 1:159222/250‑1 (MQ=255) tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGTAGGGGCGAGGGGGGTTATATTATCTGAGTGAACT‑GGGGATTAAGTTTTCGCGTAC‑ACGCGGGTTTGTTTTTGCGGAGGATGATATTGAAGGTCGGCT‑GATTGGt > 2:610390/1‑250 (MQ=255) gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGGGGGGTTATCTTTCCTGACTGAAAT‑GGCGATTAAGTTTTCGCGTAC‑CCGGCGGTTTGTTTTTGTGGGTGATGATTTTTAAGCGTGGCT‑GATTGGttg > 2:241765/1‑249 (MQ=255) taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAAT‑GGCGGTTAAGTTTCCGCGTAC‑CCGGCGGTTAGTTATTGCGGGTGATGATATTGAAGCTCGGCT‑GATTGGTTCa > 1:71192/1‑250 (MQ=255) aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGCTTAGTTATTGTGGATGGTGATATTGAAGCTCGGCT‑GGTTGGTTTAt > 1:31103/1‑250 (MQ=255) aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGTCATGAGAAGTGAGGGCAGAGAGGGATAATCTTGCCTCACCGGACA‑GGCGATTAAGGTTCCTCGTCA‑CCGGGGGTTAGTTATTGCGGATGATGATATTTAAGTTCGGGT‑GAGTgggtttt > 2:130763/1‑245 (MQ=255) tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTTTCATGCGAAGTGAGCGCCGAGAGGGGGTATTTTTCCTGACTGAACT‑GGCGGTTAAGTTTTCGCGTAC‑CCGGCGTTTAGTTTTTTCGGGTGATTATATTTAAGCTCGGCT‑GATTGGTTTCTTGTctgcc > 2:404328/1‑246 (MQ=255) gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCa < 1:542587/250‑1 (MQ=255) ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTCAGTAGTGCGCCCAGAGCGGGGTTATTCTTCCTGACCGACCT‑GGCGCTTTAGTTTTCGCGTACTCGGGCGTTT‑GTTATTGTGGACGATGATCTTTACGCTCCGCC‑GTTTTGTTCATTTTTGCTCTCACCGc > 2:145960/1‑250 (MQ=255) ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTgg < 1:433300/250‑1 (MQ=255) ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTg < 1:560689/249‑1 (MQ=255) gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑a < 1:42394/250‑1 (MQ=255) tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTaaa < 1:179546/250‑1 (MQ=255) ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCa < 1:398643/248‑1 (MQ=255) ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCa < 1:384677/248‑1 (MQ=255) tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGAtt < 1:168484/250‑1 (MQ=255) cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTGCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGAttttt < 2:30828/250‑1 (MQ=255) ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGGTAAGGGAGGGCAGGGAGGGGTTATTTTTGCTTACTGAACT‑GGCGATTAAGGTTTCCCGTAC‑CCGGCGGTTTGTTTTTTCGGGTGGTGAAATTGTAACCCGGCG‑GGTTGGGTTATTGTTGCCCTCTCCGCTTGGCGGTGTGATTAA‑TAAAGCGTCCACGCTGGAGGTTTTttatgc > 2:483193/1‑248 (MQ=255) ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGGTTCCGCGTAC‑CCGGCGGTTAGTTATTTCGGGTGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGGTTTTTTATCAGGAActc > 1:49242/1‑250 (MQ=255) tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCtttt < 1:377838/250‑1 (MQ=255) aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGTCCAGAGAAGAGAGGGGAGAGGGGGGTTATTTTGTCTGACTGAACTGGGGGGTTAAGTTT‑CGCGTAT‑CACGCGGTTAGTTATTGCGGCGGATGGTATTTAAGATCGGGTGGTTTGGTTC‑TTGTCGCCATCACCCCTGGGGGGGGG‑GTTAAGTAAAAGGTATAAGACGGAGATTTTTTATAAGGAGATCtcttttttct > 2:135803/1‑244 (MQ=255) aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCAtt < 1:411776/250‑1 (MQ=255) aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCCGGGAGGGGTTATCTTGCCTGACTGAACT‑GGCGGTTAAGTTTTCCCGTAC‑CCCGCGGTTTGTTTTTTCGGATGATGATATTGAAACTCGGCT‑GGTTGGTTTATTTTCGCCATCACCGCTGGACGGGGG‑TTTTAGTAAAGCGTCAACGCTGGAGATTTTTTTTTAGGAACACTTTTTTTCATTaa > 2:30627/1‑249 (MQ=255) aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAAT‑GGCGATTAAGTTTTCGCGTAC‑CCGGGGTTTTGTTTTTTCGGAGGAGGATATTTAAGCTCCGCT‑GTTTGGTTCATTGTTGCCATCACCGCCGGACGGTGG‑TTTTAGTAAAACGTTTACCCTGGAGGTTTTTCATCAGGGAATCTTTTTTTCTTTata > 2:532249/1‑248 (MQ=255) aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGGGAGGGGTTATCTTTCCTTATTGTACT‑GGCGATTTAGGTTTCCCGGAC‑CCGGCGTTTTGTTATTGCGGGTGATGGTATTTTAACTTGGCT‑GGTTGGTTCATTTTTTCCCTCACCCCTGGGCGGTGG‑ATTTAGTAAAGCGGCAACGCTGGGGATTTTTTATCAGGGACTCTTTTTTTCATTAAATGgtt > 2:174269/1‑249 (MQ=255) aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 2:369878/250‑1 (MQ=255) tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACT‑GGCGGTTAAGTTTCCGCGTAC‑CCGGCGGTTTGTTATTGCGGGTGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGGGT‑ATTAAGTAAAGCGGCAACGCTGGAGATTTTTTATCAGGAACTCTTTTTGTTATTAAATGgtg > 1:187618/1‑249 (MQ=255) gATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAg < 1:182233/250‑1 (MQ=255) gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcg < 2:526945/247‑1 (MQ=255) ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccga > 2:364457/1‑250 (MQ=255) gcggTCATTTTTTCTTTTTCTGCCACGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccgac < 1:243339/250‑1 (MQ=255) tgTCCTTTTTTCTTTTTCTGCCCTGAGACGTGAGCGCCGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGaa > 2:581227/2‑205 (MQ=255) ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTg < 2:71000/250‑1 (MQ=255) gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTg > 1:239083/1‑249 (MQ=255) aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAAc > 1:244679/1‑249 (MQ=255) aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa < 2:518511/250‑1 (MQ=255) tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAAt > 1:162937/1‑248 (MQ=255) ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAg > 2:510312/1‑250 (MQ=255) | GATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACT‑GGCGATTAAGTTTCCGCGTAC‑CCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT‑ATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAG > NZ_CP009273/4593914‑4594408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |