Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,089,5710AG41.3% 60.8 / 12.5 47I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base A (6/21);  new base G (19/0);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 5.07e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGG  >  NZ_CP009273/2089331‑2089811
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
gCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc                                                                                                                                                                                                                                             <  1:320833/248‑1 (MQ=255)
   aaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTTAGGCTCTgg                                                                                                                                                                                                                                        >  2:188214/1‑250 (MQ=255)
            aTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTGTTTGGGAggttgcg                                                                                                                                                                                                                               >  2:139103/1‑244 (MQ=255)
            aTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCcg                                                                                                                                                                                                                               <  1:228608/250‑1 (MQ=255)
             tCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCcgc                                                                                                                                                                                                                              <  1:191309/250‑1 (MQ=255)
                                         ggtggtGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGGAAATTCTTTTCTTTTGCGCGGATaa                                                                                                                                                                                                   >  1:423263/1‑249 (MQ=255)
                                            ggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGCGCGGGTAAAAtt                                                                                                                                                                                               >  1:472373/1‑250 (MQ=255)
                                               gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGCGAAAATTCTTTCCTTTTGCGCGGATAAAAttttc                                                                                                                                                                                            >  1:441696/1‑248 (MQ=255)
                                                         cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGGAGATGCCGCGAAAATTCTTTTCTTTGGGGCGGAGAAAATTTACATCAACtctc                                                                                                                                                                                  >  1:377368/1‑250 (MQ=255)
                                                                 cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTGGGAGATGCCGCGCAAAATATTTTCTTTTGGGCGGGGAAAATTTTCCTCAACTACCCTCCGCTg                                                                                                                                                                          >  2:313358/1‑250 (MQ=255)
                                                                   aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTCCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGc                                                                                                                                                                        >  1:37109/1‑250 (MQ=255)
                                                                    ccATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCa                                                                                                                                                                       <  1:524301/250‑1 (MQ=255)
                                                                      aTGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGa                                                                                                                                                                     <  1:462527/250‑1 (MQ=255)
                                                                       tGAAATCGTTGGCGAGATCGTGCCGCTGGGAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAc                                                                                                                                                                    <  2:175094/250‑1 (MQ=255)
                                                                                tGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCGCGAAAATTCTTTCCTTTGTCGCGGGGAAAATTTTCATCAAATCTCCTGCGCTGGCCGAACCCACatt                                                                                                                                                            >  2:156702/1‑249 (MQ=255)
                                                                                     atatCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTAc                                                                                                                                                      <  2:488341/250‑1 (MQ=255)
                                                                                        tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCg                                                                                                                                                   <  2:127401/250‑1 (MQ=255)
                                                                                           tGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTTAGTCTCTGGAAGATGCCGCGGAAATTTTTTTCTTTTTCGCGGGTGAAAATTCCATCAACTCTCCTCCGCCGGCCTACCCCACATTAATTACTCACCt                                                                                                                                                >  2:436879/1‑250 (MQ=255)
                                                                                                cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCg                                                                                                                                           <  2:137297/250‑1 (MQ=255)
                                                                                                   gcaaatctgtacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGGTATCACCGCATCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTAGTCGACATTCCGTTTTGTGTGGCGGGCGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTAGCAGACCCAACATTATTTCCTCGCCTGGCCGATc                                                                                                                                        <  2:553063/241‑1 (MQ=255)
                                                                                                       aaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCGCTGGGAAATGCCGCGGAAAATTTTTTCTTTTGCGCGGGTAAAAATTTCACCAACACTCCTGCGCTGGCGGACCCCACATTAATTACTTGCCTGGcccaaccctt                                                                                                                                    >  2:437644/1‑242 (MQ=255)
                                                                                                         cgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGGCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGTGCTGGGAGACAGCTCGCAAAGACTTTTCTTTGGGGCGGGAAAAATTTTTATCCAGTCCACTGCGCTGGGAAGCCCCACATATATTTATCACCTTGCCGaaccggttg                                                                                                                                  >  2:398559/1‑242 (MQ=255)
                                                                                                                 tGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTGGCGCGGATAAAAATTTCATCCACTCCCCTGCGCTGGCAGACCCCACCATTATTACTCCCCCGGCCCACCGCTTTGGCGTGccg                                                                                                                          >  2:432464/1‑248 (MQ=255)
                                                                                                                   aagaagGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCCCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAATCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAgtg                                                                                                                        >  1:562270/1‑250 (MQ=255)
                                                                                                                                 gAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTa                                                                                                          <  2:570029/250‑1 (MQ=255)
                                                                                                                                    cTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTg                                                                                                       <  2:161509/250‑1 (MQ=255)
                                                                                                                                              aCGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCCCCGGGAAAAGCCCCGCAAATTTTTTTCTTTTGGGCGGGTAAAAATTCCTTCCAACCTCCTTCCCCGGCCCACCCAACATTTATTATTCGCCCGGCCGATCGCCTTGGCGTGCCGAGCATTGTGGTCGGGATTgtttaccggga                                                                                             >  2:140369/1‑240 (MQ=255)
                                                                                                                                               cGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTgg                                                                                               <  2:587297/247‑1 (MQ=255)
                                                                                                                                                 atatCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTTGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGGGCAGGGTATTGTGGTCGGGGTTGATACCTGGTacga                                                                                          >  1:420836/1‑250 (MQ=255)
                                                                                                                                                          cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAAc                                                                                <  2:189730/250‑1 (MQ=255)
                                                                                                                                                               aGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGAACCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGGCGGGATTGATACCTGGTACGACGCCG‑AAACCggg                                                                            >  1:391557/1‑248 (MQ=255)
                                                                                                                                                                      gCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATc                                                                     <  1:38569/249‑1 (MQ=255)
                                                                                                                                                                            tgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTTGAAAATGCCGCGAAAAATCTTTTCTTTTGCGCGGATAAAATTTCCCTCAAATCTCCCGCGCTGGCCGACCCCACATTAATTAATCGCCTGGCCGAACGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTTGGACGGCGCCGCCAACCGG‑AAATATCATGTG‑a                                                              >  2:485822/1‑249 (MQ=255)
                                                                                                                                                                            tgGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑a                                                              <  2:348778/249‑1 (MQ=255)
                                                                                                                                                                                  aTAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAata                                                       <  2:119279/250‑1 (MQ=255)
                                                                                                                                                                                        gCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCgg                                                 <  1:122185/250‑1 (MQ=255)
                                                                                                                                                                                         cTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTTCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGGAAATATCATGTG‑AATCAATATACCCGc                                                >  1:142381/1‑250 (MQ=255)
                                                                                                                                                                                            ggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCCGGGAGATGCCGCGAAAATTCTTTTCTTTGGCGCGGGTAAAATTTCCATCAACTCCCCCGCGCTGGCAGACCCAACATTAATTACTCGCCTTGCCGCTCGATTTTGCGGGCAGTGTATTGTGGTCGGGATTGTTATCCGGTACGACGCCG‑CAAACGGGTAATTTCATGTG‑AATCACTAAATCGGCCAt                                             >  2:479615/1‑250 (MQ=255)
                                                                                                                                                                                                        ggCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGCGTAAGATTCTGGGAAGTGCCGCGAAAAATTTTTCCTTTGGCGGGGATAAAATTTCCCTTCACTCTCCTGCGCTGGCAGAGCCCACATTATTTTCTCCCCTGGCCGATTCATTTTGCGTGCAGCGTATTTTGGGGGGTGTTTAGACCCGGCACGGCGCCG‑AACCCGGGAAATATCATGTGTAATCAA‑ATACCCGCGAGCGAAGCCGCaac                                 >  2:245540/1‑248 (MQ=255)
                                                                                                                                                                                                            gAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGGTAAAGCTCTTGAAGATGCCGCGAAAATTTTTTTCTTTGGGGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCCGACCCAACATTAAATACTCGCCTGGCCGGTCGCCTTGGCGTGGAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATAACGGCGAGGAAAACCGCACCcgcg                             >  2:359347/1‑250 (MQ=255)
                                                                                                                                                                                                                  aTCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGAAGCCGCGAAAATTCTTTTCTTTGGGGCGGATAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTTTGGTCGGTATTGATACCCGGTACGACGCCG‑GAACCGGTAAATATCATGTG‑AATCAATATACCGGCGGTGAAAGCCCCACCCCCGTCACt                        >  2:524617/1‑249 (MQ=255)
                                                                                                                                                                                                                  aTCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACt                        <  2:441696/249‑1 (MQ=255)
                                                                                                                                                                                                                   tCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGAAGCCGCGAAAATTTTTTCCTTTGGGGCGGGTAAAATTTTCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCCCTTTGGCGTGCAGTGTATTGTGGTCGGGATTGATACCTGGTACGACGCCG‑AAACCGGGAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCCCGGCACTc                       >  2:571042/1‑249 (MQ=255)
                                                                                                                                                                                                                                  gtgtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCCCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGGGCAGTGTATTGTGGTCGGTATTGATACCTGGGACGACGCCG‑AAACCGGTAAATATCATGGG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCCTCACTCAATGGGAAAAGCCCGa       >  2:177723/1‑250 (MQ=255)
                                                                                                                                                                                                                                     tgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGCCTg    >  1:294801/1‑250 (MQ=255)
                                                                                                                                                                                                                                       gCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTggg  <  1:466758/250‑1 (MQ=255)
                                                                                                                                                                                                                                       gCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTgg   <  2:499403/249‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
GCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCG‑AAACCGGTAAATATCATGTG‑AATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGG  >  NZ_CP009273/2089331‑2089811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑