Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,167	0	G	T	37.7%	67.6 / 13.1	53	intergenic (+40/+25)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base G (20/13);  new base T (6/14);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAA  >  NZ_CP009273/2144920‑2145412                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                         cGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑tt‑gtt                                                                                                                                                                                                                                                        <  1:535132/250‑1 (MQ=255)    cATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTc                                                                                                                                                                                                                                                     <  1:95085/250‑1 (MQ=255)      tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTTATTCTTTTTTTGTTACCTATCCAAAAAAAAAAACCGACGAGCTAAGAGCAAAAATAATTCGCTTATCAACCGGTATATTTATTTTTTTAAC‑TTGCGGGT‑GGA‑tt‑tttttttt                                                                                                                                                                                                                                                   >  1:553788/1‑245 (MQ=255)        tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑Ca                                                                                                                                                                                                                                                <  1:39860/250‑1 (MQ=255)            ttGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAAcc                                                                                                                                                                                                                                             <  1:592979/249‑1 (MQ=255)            ttGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCg                                                                                                                                                                                                                                            <  1:345351/250‑1 (MQ=255)              ggCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGc                                                                                                                                                                                                                                           <  2:95494/249‑1 (MQ=255)                                         gTTAAAAAGGTAAAATAGGGCCAGGAGTGAAAAATAAAGAAAGAAGATATTTCAGACTGGA‑TGTATCCGCGGGGGAAAAAAATTTACGGCGT‑TTATCCCATTGATCTTTTATTGGCCCCCTACCAAAAAAAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCACCCTTTATATTTATTTTTAAACC‑GGGCGGTT‑GAT‑TT‑TTTTTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAgcg                                                                                                                                                                                                                <  1:287994/249‑1 (MQ=255)                                            aaaaaCGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGT‑TTATACCATTGATCCTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAATCAGCCTTTATATTTATTTTTTAAAC‑TGGCGGTT‑GAT‑TT‑GTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                                            <  1:463205/248‑1 (MQ=255)                                            aCAAACGTAGAATAGGGCAAGGAGTGAAAAAACAAGAAAGAAGATATTTCAGACTGGA‑TGTATCCGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCTTTTATGGGTCACCTACCAAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCTTTATATTTATTGTTTAAAC‑GGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCATAGCGCCa                                                                                                                                                                                                             <  1:485845/249‑1 (MQ=255)                                                          gggCAAGGAGTAAAAAAAAAAGAAAAAAATTTTTACAGACGGTA‑TTTATCCGCGGGGGAAAAAAATTAACGGGTT‑TTATCCCTTTGTTCTTTTTTTGGTCCCCTACCAAAAAAAAAAAGCCGACGAGAAAAGAAAAAATTTATTTCCATAACCAGCTTTTTTTTTTTTTGTTAAACC‑GGGGGGTT‑AAT‑TT‑TTTTTCTT‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTat                                                                                                                                                                                             <  2:11494/249‑1 (MQ=255)                                                                ggAGAGAAAAATAAGAAAAAAAGATATTTAAGATTGG‑‑TGTAATCGCGGGGGGAAAAAAATTACGGGGGTTTATACCTTTAATCTTTTTTTGCCCCCCTACCAAAAAAAAGACGCCGAGGAGCAAAAACAAAAATTATTTCGCTAACCAGCCTTTTTTTTTTTTTTTAAACC‑GGGCGTTT‑GAT‑TT‑GTTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTatcatcat                                                                                                                                                                                       <  2:117656/250‑1 (MQ=255)                                                                      aaaaaTAAAAAAAAAAGTTA‑TTCAAGCTGGATTGAATCCGGGGGGGAAAAAAATTTCGGGGGT‑TTATACTTTTTATCTTTTTTTGGCCCCCTATCAAAAAAAAAAACCCGCGGAACTAAGAGAAAATTTATTTCGTTAACCACCTTTTAT‑TTTTTTTTTTAAAC‑CGGGGGTT‑TAATTT‑TTTGTCTC‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTAc                                                                                                                                                                                 <  2:113305/249‑1 (MQ=255)                                                                                aaaaaaGATATTTCAGACGGGA‑TTTATGCGCGGGGGAAAAAAATTTCGGGGGG‑TTATACCTTTTATCTTTTATGGGCCCCCTACCAAAAAAAAGAAGCGGACAAGCAAAGAAAAAAATTAGTCCTCTAATCAGCCGTTATTTTTTTTTTTAAACC‑GGGGGGTT‑GAT‑TT‑GTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTAc                                                                                                                                                                       <  2:373074/250‑1 (MQ=255)                                                                                                                               aCGGGGT‑TTATACATTTGATCTTTTATTGTTCCCTTACCAAAAAAAAAAACCCGACGAGCAAAGAAAAAAATTAGTTCGCAAATCAGCTTTTATATTTATTTTTAAAAC‑GGGCGTTT‑AAT‑TT‑TTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                                                                                                                         <  1:353955/249‑1 (MQ=255)                                                                                                                                          tcccATTGACCTTTTATGGGTCCCCTACCAAAAAGAAAAGGCCGACGAGCTAAGAAAAAAATTTGTTCTCTAATCAGCCTTTATTTTTATTGTTAAACC‑TGGCGTTT‑GAT‑TT‑TTTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCt                                                                                                              <  1:658997/248‑1 (MQ=255)                                                                                                                                          taCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCCGG‑CTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCt                                                                                                              >  1:501860/1‑250 (MQ=255)                                                                                                                                            ccATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTTCTGc                                                                                                            >  1:139391/1‑250 (MQ=255)                                                                                                                                             cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CCACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAATGCC‑CACGTTTTATCATCATCCCTACGCTCGTTTACCGCCCGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg                                                                                                           >  1:72893/1‑250 (MQ=255)                                                                                                                                                 gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑TTA‑TT‑TTTGTTTT‑CTATCCACATGAGAAACTTACTCTTCCCACATCGCCAGGGCCTAACATTTT‑TCATCCTCCCATCGCTTCATTACTCCCCGG‑CCTGTCAAAAACCGCTTTGATAATTTAGGGAATAAATCGTATTGTTCCTGCGTtgc                                                                                                       >  1:663729/1‑250 (MQ=255)                                                                                                                                                          ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCa                                                                                                                                                                                                             >  1:207050/1‑141 (MQ=255)                                                                                                                                                           tgcaccccTACCAAAAAAAAGAACCAGAGGAGCAAAGAGAAAATTTATTTCGTTAACCTGCTTTTATATTTTTTG‑TTAAAC‑CGGGGGTT‑TAATTT‑GTGGTTTC‑CAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg                                                                                              <  2:671503/243‑1 (MQ=255)                                                                                                                                                                aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑TTTGTCTT‑CAACCGCCATGCGAAACTCACTCTGTCCACAGAGCCCGTTCC‑CAAGCTTTATCATCATTCGTACGCACGCTTACCGCCCGG‑CTGGACACAAAACGCCTTAACAACTCAGGTAATAATTCGCGTTATTCCTGCCTTTCTGCCGCCAGTTCAtt                                                                                        >  2:285492/1‑250 (MQ=255)                                                                                                                                                                aCCTACCAAAAAAAAAAAGCCGACGAGCTAAGAAAAAATTTAGTCCGTTAATCAGCCTTTATTTTTTTTTTTAAAAC‑GGGCGTTT‑GAA‑TT‑TTTGTCTT‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCa                                                                                          <  1:461548/248‑1 (MQ=255)                                                                                                                                                                 ccaaccaaaaaaaAGAGGCCGACGAATAAAAAGAAAAATTATTCCGTTAACCAGCTGTTATTTTTATTTTTAAAAC‑GGGGGGTT‑AAT‑TT‑TTTTTCTC‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTg                                                                                       <  1:483127/243‑1 (MQ=255)                                                                                                                                                                  cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑TTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AAAGTTTTATCATCATCCGTACGCCCGGTTACCGCCCGG‑CTGGACCAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCAGTcccatg                                                                                       >  2:636995/1‑245 (MQ=255)                                                                                                                                                                   tATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAAACGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGGGCC‑AACGTTTTTTCATCATCCGTACGCTCGTTTTCCGCCCGG‑CCGGACAAAAAATGCTTTTACAATTTAGGGAATAAAACGAGTTTTTCCTGCGTTTCTGCCGCCAGTCCacttg                                                                                      >  2:617172/1‑245 (MQ=255)                                                                                                                                                                        aaaaGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACg                                                                                >  2:420291/1‑250 (MQ=255)                                                                                                                                                                             aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑TAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑CACGTTTTATCATCATCCGTACGCTCGTTTACCCCCGGG‑CTGGACAAAAACTGCTTTTACAAATCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAGTCCATTGAAAAACGGGGt                                                                            >  1:521298/1‑249 (MQ=255)                                                                                                                                                                                  gCCGAAGAGATAAAAGAAAAATTGTTTCTTTACTCAGCTTTTATTTTTTTTGTTAAAAG‑GGGGGGTA‑GAT‑TT‑GTTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaa                                                                      <  1:330645/250‑1 (MQ=255)                                                                                                                                                                                  cccGACGAGAAAAGAAAAAAATTAGTCCGATAAGCACCCGTTATATTTATTTTTAAAAC‑GGGGGTTT‑GAT‑TT‑TTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaa                                                                      <  1:564977/249‑1 (MQ=255)                                                                                                                                                                                     gcagagaaaaGAGAAAAATTATTCCGCTCACCAGCTGTTTTATTT‑TTTTTTAAACGGGGCGGTT‑AAT‑TT‑GTTTTCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAgg                                                                   <  2:48511/242‑1 (MQ=255)                                                                                                                                                                                        gaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                                                 >  1:651038/1‑249 (MQ=255)                                                                                                                                                                                              agaaaaaaaTTATTTCGAAACTC‑GCCTTTTTTTTTTTTTTTTAAAA‑GGGGGGTTAGAT‑TT‑TTTTTCTA‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                                          <  2:461938/245‑1 (MQ=255)                                                                                                                                                                                              aaaaaaaatTTATTTCTCAAAACACCCTTTATTTTTTTTGTTAAACG‑GGGGGGTT‑AAT‑TT‑TTTGTCTA‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                                          <  1:471750/245‑1 (MQ=255)                                                                                                                                                                                                   aaaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑TTTTTTTT‑CAAACGCCATGAAGAAATCACTCTCCTCACAGCGCCCGATCC‑AACCTTTTTTCATCATTCCTACGCCCGTTTTCCGCCCGG‑CTTGACAAAAACCGCTTTTAAAATTAAGGTAATACATCGAGGTGTTCCTGCGGTTCTTCCGCCCGTTCCTTTGAAAACGGGGGAAAGAAAGGCACATCAGGAGAAt                                                     >  2:268316/1‑250 (MQ=255)                                                                                                                                                                                                       ttAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCCGG‑CTGGACAAAAAATGCTTTAACAAATCAGGTAATAAATCGAGTTGTTCCTGCGGTGCTGCCGCCAGTCCCTTGAAAAACGGGGTAAAGAAAGGCAAATGGGGAGAATTATc                                                 >  1:407606/1‑250 (MQ=255)                                                                                                                                                                                                        tAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑TGA‑TT‑TTTTTCTT‑CAACCGCCCTTAGAAACTCCCTCTGCCCCCCGCGCCCGTGCC‑CAAGGTTTTTCATCATCCCTACGCCCGTTTTCCCCCGGGCCGGGACAAAAA‑TGCTTTTACAATTCAGGTAATAAATCGAGGTTTTTCTTCGTTTCTTCCCCCCGTTCATTGAAAAACGGGGGAAAGAAAGGGAACTgagggaaaatattc                                                >  2:458853/1‑240 (MQ=255)                                                                                                                                                                                                             cGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTTTCTT‑CAACCGCCATGAGGAAATCACTCTGCCCACAGCGCCAGTGCC‑CACGTTTTATCATCATCCGTACGCCCGTTTACCCCCGGG‑CTGGGCAAAAACTGCTTTAACAAATCCGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAAGCACATGAGGAGAATTATCCAGCa                                            >  2:421432/1‑249 (MQ=255)                                                                                                                                                                                                                 aaTCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCCTTTACCGCCGGG‑CTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAAAGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                                        >  2:273429/1‑249 (MQ=255)                                                                                                                                                                                                                 aaTCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGCGTTGCTGCCGCCAGTCCCTTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                                        >  1:301064/1‑249 (MQ=255)                                                                                                                                                                                                                  tccggCCTTTATTTTTTTTTTTAAAAC‑GGGGGTTT‑AAT‑TT‑TTTGTCTA‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTa                                      <  1:427131/246‑1 (MQ=255)                                                                                                                                                                                                                       cttttATATTT‑TTTTTTAAAC‑CGGGGGTT‑GAA‑TT‑TTTTTCTTAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACg                                   <  1:593854/245‑1 (MQ=255)                                                                                                                                                                                                                        cGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑TTTGTTTT‑CAAACTCCATGAGAAAATTACTCTGCCCACAGCGCCAAGGCC‑AACGTTTTTTCATCATCAGTACGCGCGTTTACAGCCGGG‑GTGGAAAAAAACTGCTTTAACAAATCAAGGAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCAGTGAAAAACGGGGTAAAGAAAGGGACAATAGACGAATTATCCAGCATATTTAACGcac                                >  2:133323/1‑248 (MQ=255)                                                                                                                                                                                                                                 tattGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AAAGTTTTATCATCCTCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                       >  1:539782/1‑250 (MQ=255)                                                                                                                                                                                                                                   ttGTTAAAAC‑GGGGGTTT‑GAT‑TT‑TTTCTTTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGAt                      <  1:277904/249‑1 (MQ=255)                                                                                                                                                                                                                                          aaC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCCGCATATTTAACGCCAGTCGCTGGATACCATCCg              >  2:594120/1‑250 (MQ=255)                                                                                                                                                                                                                                            c‑TGGCGTTT‑GAT‑TT‑TTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AAc                                                                                                                                                                                                    <  1:226141/67‑1 (MQ=255)                                                                                                                                                                                                                                              ggggtggt‑GAA‑TTGGTTGTCGC‑CACCCGCCAGAAGAAACTCCCTCGCCCCCCAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAGACGGGGTATAGAAAGGCACATGAGGAGAATTATCCAGCATATGTAACGCCAGTCGCTGGATACCATCCGt             <  2:327867/242‑1 (MQ=255)                                                                                                                                                                                                                                               ggCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTaaa          >  2:598904/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtgct      >  2:260671/1‑250 (MQ=255)                                                                                                                                                                                                                                                    tt‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtgctg     >  1:297268/1‑250 (MQ=255)                                                                                                                                                                                                                                                       gAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtgctgc    >  2:387765/1‑249 (MQ=255)                                                                                                                                                                                                                                                        aa‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAGCGCCAGTCGCTGGATACCATCCGTAAATGCTGCaa  >  1:674715/1‑250 (MQ=255)                                                                                                                                                                                                                                                        aa‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCaa  >  1:402417/1‑250 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                         CGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTT‑GAA‑TT‑GTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC‑AACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGG‑CTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTGCAA  >  NZ_CP009273/2144920‑2145412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑