Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,344,203	0	A	C	40.6%	126.2 / 14.4	69	L728V (TTA→GTA)	arcB	aerobic respiration two‑component sensor histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base A (37/4);  new base C (0/28);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 2.14e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCC  >  NZ_CP009273/3343954‑3344447                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     tGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTaa                                                                                                                                                                                                                                                      >  1:655885/1‑250 (MQ=255)    tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGCTGGCGTAAccc                                                                                                                                                                                                                                                   >  2:427018/1‑250 (MQ=255)         ccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACACTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGTCGTTATCTTCCCAGACCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTg                                                                                                                                                                                                                                              <  1:323188/250‑1 (MQ=255)            tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcc                                                                                                                                                                                                                                           >  1:419669/1‑250 (MQ=255)             gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAccc                                                                                                                                                                                                                                          >  1:452230/1‑250 (MQ=255)              tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                                                                                                         >  1:195827/1‑250 (MQ=255)              tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                                                                                                         <  1:358601/250‑1 (MQ=255)                aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgc                                                                                                                                                                                                                                       >  2:460797/1‑250 (MQ=255)                   catcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCcagccg                                                                                                                                                                                                                                     >  2:28013/1‑249 (MQ=255)                         aatattTTGCGGCCCAGCCAAGTTTTCCCGGGTGTTGGCGCAGTTTCCGCGCCCCCCGTTCTAGCGGGGGTTTTTTTTTAGTGGCTTTTC‑CCCCCCCCGCTTTCACCATTTCTCCGCCGTGCCGCCCCTCTTCTTTCATCTCTCCAATCCATCCCCCGCCGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCttt                                                                                                                                                                                                                              <  2:442332/250‑1 (MQ=255)                               ttACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTAGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAAtttt                                                                                                                                                                                                                        >  2:8430/1‑250 (MQ=255)                                     cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtcc                                                                                                                                                                                                                  >  1:9416/1‑250 (MQ=255)                                        aGCCAAGATTTCCCTGTTTTGGGCGCATTATTCGCGCCCCCCGGTCTAGCCGGGGTTATTTTTTAGTGGCTTTTG‑CCCCCCACGCTTTCACCCTTTCTCCGTCGTGCCCCCCCTCTTCTTTCATCTCTTAAATCCATCCCCCGCCTTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGATTTTCCTCACCCAGTTTTTGCAGATGGCATACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGtccttc                                                                                                                                                                                                               <  2:617321/250‑1 (MQ=255)                                             aGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaa                                                                                                                                                                                                          >  2:675544/1‑250 (MQ=255)                                                      tGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                                                                                                                 >  2:569773/1‑250 (MQ=255)                                                        gtgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                                                                                                               >  1:602004/1‑250 (MQ=255)                                                         tgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGGGGCTTTGG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCCCTCTTCTTTCATCTCTCCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTGGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                                                                                                              <  1:60039/250‑1 (MQ=255)                                                                gcAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTCTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTg                                                                                                                                                                                       >  1:86231/1‑250 (MQ=255)                                                                  aGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcg                                                                                                                                                                                     >  2:88033/1‑250 (MQ=255)                                                                      ttCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                                                                                                                 >  2:430666/1‑250 (MQ=255)                                                                       gcgccccccccGGTCTGGCGGGGTTCTTTTTTTAGTGGTTTTTG‑CCACCCCCGCTTCCACCGCTTCTCCGTCGGGACCCCTCTCTTTTTCCATCTCTTCAACCCTCCCCCCGCCTTTTTCTCCCCGGGCGGGAAGGCCAGGAGCCTAATTTTGCTGCCCCATTTTTTGCAGATGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                                                                                                                 <  2:688018/242‑1 (MQ=255)                                                                                ccGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGAc                                                                                                                                                                       >  2:455423/1‑250 (MQ=255)                                                                                 cGGTCTAGCCGGGGTCTTTTTTTAGTGGCTTTTC‑CCCCCCACGCTTCCAGCACTTCTACGTCGTGACCCCACTCTTCTTCCATCTCTTCAATCCATTCACCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACt                                                                                                                                                                      <  1:388686/250‑1 (MQ=255)                                                                                 cGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACt                                                                                                                                                                      >  1:553081/1‑250 (MQ=255)                                                                                             ggTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGa                                                                                                                                                          >  2:169209/1‑250 (MQ=255)                                                                                                 tttttttTATTGCTTTTGCCCCCCCCCGCTTCCACCTCTCCTCGTTCGTGCCCCCCCTCTTCTTCCTCCTCTTCACTCCATCCCCCGCCTTTTTCTTCCCAGGCCGGAAGGCCGGGACACTGATTTTCCTCCCCCAGCTTTTGCAGATGGCGCACCCCCACCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACa                                                                                                                                                        <  2:8698/248‑1 (MQ=255)                                                                                                   tttttAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGc                                                                                                                                                    >  2:302278/1‑250 (MQ=255)                                                                                                               tttG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtct                                                                                                                                        >  1:4346/1‑250 (MQ=255)                                                                                                                    ccacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                                                                                                                     >  2:355791/1‑249 (MQ=255)                                                                                                                       cccccGTTTACACCACTTCGCCGTCGTACCGCCACTTTCTTTTCACCCCTCCAACCAATACACCGACGTTATCTCCCCGGGCCGGAGGGCCAGGAGACTGATTTGCCTGCCCCAGCTGTTGCAGTTGCCGAACCCCCCCGCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                                                                                                                 <  2:140253/250‑1 (MQ=255)                                                                                                                       cccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                                                                                                                 >  2:518162/1‑250 (MQ=255)                                                                                                                        ccccGCTTTCACCACTTCTCCGTCGTGCCCCCCTTCTTCTTTCACCTCTCCAACCCATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGCTGTTGAAGATGGCTTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAac                                                                                                                                <  1:353903/247‑1 (MQ=255)                                                                                                                         cacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAaca                                                                                                                               >  2:74699/1‑250 (MQ=255)                                                                                                                             cTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGc                                                                                                                           >  2:535037/1‑250 (MQ=255)                                                                                                                             cTTTCACCCCTCTTACCTGGTGCCCCCACTCTTCTTTCCTCTCTACAATCCCTCCCCCCACGTTCTCTTCCCAGGCCGGAAGGTCAGGAGACTGATTTGGCTGACCCAGTTGTTGCAGATGGCAAACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACAcc                                                                                                                             <  2:636702/248‑1 (MQ=255)                                                                                                                                      cTTCTACGTCGTGACCCCACTCTTCTTTCATCTCTTCAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTc                                                                                                                  <  1:460797/250‑1 (MQ=255)                                                                                                                                         cTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGt                                                                                                               >  1:467952/1‑250 (MQ=255)                                                                                                                                             gtctgacccccACTCTTTTTTCCTCTCTCAAACCCATTCCCCGCCTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTCACCCTGTTGTTGCAGATGGCGCACCCCCACCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                                                                                                           <  2:401385/241‑1 (MQ=255)                                                                                                                                             gtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                                                                                                           >  2:431450/1‑250 (MQ=255)                                                                                                                                               cgtGACCCCCCTCTTCTTTCATCTCTTCAATCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTGTTGAAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAg                                                                                                         <  1:184681/250‑1 (MQ=255)                                                                                                                                                         ctctTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTcc                                                                                                >  1:125560/1‑249 (MQ=255)                                                                                                                                                              cttCCACCTCTACAATCCATTCCCCGCCTTTATCTCCCCAGGCCGGAAGGTCAGAAGACTGATTTTGCTGCCCCAGTTGTTGCAGATGGCTTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTAc                                                                                             <  1:94530/247‑1 (MQ=255)                                                                                                                                                                      tctTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCgaga                                                                                   >  1:9432/1‑249 (MQ=255)                                                                                                                                                                          cAACCCATCCACCGCCGTTATCTCCCCAGGCGGAAAGGACGGGAGACTAAATTTCCTGCCCCAGTTGTTGCAGAGGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTg                                                                              <  2:257206/250‑1 (MQ=255)                                                                                                                                                                             tCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTc                                                                           >  2:179979/1‑250 (MQ=255)                                                                                                                                                                               ctttCACCGACGTTATCCGCCCAGGCCGGAAGGCAGGGAGACTGAATTTGCTGACCCGGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCa                                                                         <  1:8430/248‑1 (MQ=255)                                                                                                                                                                                aTTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAg                                                                        >  1:623464/1‑250 (MQ=255)                                                                                                                                                                                 tccACCGACTTTCTTTCCCCGGGCCGGAGGGCCGGGACACTGAGTTTGCGGACCCGGCTTTTGCAGATGGCGTACCCCCCCCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGc                                                                       <  2:404256/248‑1 (MQ=255)                                                                                                                                                                                   ccccGACGTTATCTCCCAGGGGGGGAAGGGGAGAAAATGGATTTTGCCGCCCCTGTTGTTGCAGAGGGCATACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCAt                                                                     <  2:391330/248‑1 (MQ=255)                                                                                                                                                                                   acccGGCGTTACCTACCCGGCGCGGAGGGCCCCGATACGGAATTTCCTGCCCAGGCGGTGGCAGAGGGCCGACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCAt                                                                     <  2:507056/248‑1 (MQ=255)                                                                                                                                                                                       gccGTTATCTCCCCAGGCGGGAAGGTCAGGAGACTGAATTTGCTGACCCAGTTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGa                                                                 <  1:520598/248‑1 (MQ=255)                                                                                                                                                                                        aCGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGaa                                                                >  2:66521/1‑250 (MQ=255)                                                                                                                                                                                            tATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATc                                                            >  2:289313/1‑250 (MQ=255)                                                                                                                                                                                              tCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATc                                                            >  1:550069/1‑248 (MQ=255)                                                                                                                                                                                               ctccccGGGCGGGAGGGCCGGGAGACTGATTTGCCTCCCCCAGCTGTTGAAGATGGCGCACCCCCCCCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGa                                                          <  2:655885/246‑1 (MQ=255)                                                                                                                                                                                                       gCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCtt                                                 >  2:315345/1‑250 (MQ=255)                                                                                                                                                                                                        ccGGAGGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTc                                                <  1:604437/250‑1 (MQ=255)                                                                                                                                                                                                        ccGGAAGGTCAGGAGACTGAATTGCCTGCCCCAGTTTTTGCAAAGGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTc                                                <  1:491053/250‑1 (MQ=255)                                                                                                                                                                                                          gcgagggcgggAGACAGAATTGCCTCCCCCATCTGTTGTAGAGGCAGCACCCCCACCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTg                                              <  2:499796/241‑1 (MQ=255)                                                                                                                                                                                                            aaGGTCAGGAGACTGAATTGGCTGCCCCAGCTGTTGCAGATGGCGAACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGAt                                            <  2:258356/250‑1 (MQ=255)                                                                                                                                                                                                                 cggcagattcaaTTTACTGACCCATCTGTTGCGTATGGCGTCCCCCCCCCCCCTCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACt                                       <  2:586743/240‑1 (MQ=255)                                                                                                                                                                                                                         tGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCtctt                               >  1:599991/1‑250 (MQ=255)                                                                                                                                                                                                                           aaTTCGCCGCCCCAGCTGTGGAAGGAGGCCTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCtcttct                             <  2:493557/250‑1 (MQ=255)                                                                                                                                                                                                                              tgccTGCCCCGGCTGTTGCAGAGGGCGTACCCCCACGACCCCCGCCGCACCTCTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTgt                           <  2:86231/246‑1 (MQ=255)                                                                                                                                                                                                                                    aCCCATCTGTTGCAGATGCCGTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCa                      <  1:325475/248‑1 (MQ=255)                                                                                                                                                                                                                                       cAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTa                 >  1:71680/1‑250 (MQ=255)                                                                                                                                                                                                                                         gCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTAct               >  2:179645/1‑250 (MQ=255)                                                                                                                                                                                                                                          cttttGCAGATGCCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTActc              <  1:675544/247‑1 (MQ=255)                                                                                                                                                                                                                                             ttGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTCCTGTTCTCTTCTGTCGTCACCGTACTctcc           >  2:615683/1‑250 (MQ=255)                                                                                                                                                                                                                                                      gCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATcc  <  1:394574/250‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     TGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCC  >  NZ_CP009273/3343954‑3344447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑